Chinese herbal compound is playing an important role on curing human diseases.And it has been a trend that Chinese herbal compound is being used all over the world in 21 century.However,our Chinese herbal compound is ...Chinese herbal compound is playing an important role on curing human diseases.And it has been a trend that Chinese herbal compound is being used all over the world in 21 century.However,our Chinese herbal compound is facing serious challenge for the lack of canonical system of quality criterion for Chinese herbal compound so it has been a urgent problem to set up the quality control standards and reveal therapeutic basis of Chinese herbal compound.In order to give full play to the advantages of Chinese herbal compound,modern scientific and technological is used to research of Chinese herbal compound,especially the high performance liquid chromatography tandem mass spectrometry(HPLC-MS),because it is high sensitive,rapid,and obtain more information.It is very necessary that HPLC-MS is uesed to elucidate the effective components of basic substances of Chinese Herbal Compound,and endow traditional Chinese medicine with modern scientific connotation.展开更多
建立高效液相色谱检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮3种化合物含量的方法,对比评价超声辅助提取法、回流提取法、微波辅助提取法、酶解提取法4种不同提取方法对桦褐孔菌粗提物中活性成分含量的影响。结果表明,该检测方法...建立高效液相色谱检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮3种化合物含量的方法,对比评价超声辅助提取法、回流提取法、微波辅助提取法、酶解提取法4种不同提取方法对桦褐孔菌粗提物中活性成分含量的影响。结果表明,该检测方法精密度较高,3种化合物的精密度的相对标准偏差(relative standard deviation,RSD)分别为0.14%、0.06%、0.20%;重复性RSD分别为1.67%、1.98%、2.22%;在24 h内稳定性较好,其RSD分别为0.20%、0.61%、0.25%。对比不同提取方法对3种化合物的影响,发现采用超声辅助提取法得到的目标化合物产量较高,适用于桦褐孔菌中主要活性成分的提取。该研究建立的HPLC法检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮含量的结果准确。展开更多
Lemon oils are broadly used as flavoring agents in beverages,foods,cosmetics and pharmaceuticals,yet the adulteration of natural,particularly cold pressed lemon oils is very common in the industry due to its unmet dem...Lemon oils are broadly used as flavoring agents in beverages,foods,cosmetics and pharmaceuticals,yet the adulteration of natural,particularly cold pressed lemon oils is very common in the industry due to its unmet demand and high cost.Nowadays,most quality control(QC)analysis of lemon oils is conducted by gas chromatography(GC)analysis,which is far from a reliable method.Oxygen heterocyclic compounds(OHCs)in non-volatile fraction are gaining increasing attention in authentication process because of the nearly finger-printing profiles of OHCs in cold pressed citrus essential oils.Our goal in this study was to identify OHCs using high performance liquid chromatography(HPLC)in lemon oils,establish OHC profiles,perform stepwise logistic regression analysis(SLRA)and build effective predicting model and further determine adulterated lemon oils by referencing the OHC profiles and established models.After HPLC analyses,profiling and SLRA modeling of 154 OHCs samples of industrial lemon oils,we found that the combination of isopimpinellin and total OHC concentration are essential and robust predictors to differentiate authentic samples from adulterated lemon oils with a success rate of 98%from the 5-fold cross validation.This study provided a reliable and efficient method in determining the authenticity of lemon oils.展开更多
文摘Chinese herbal compound is playing an important role on curing human diseases.And it has been a trend that Chinese herbal compound is being used all over the world in 21 century.However,our Chinese herbal compound is facing serious challenge for the lack of canonical system of quality criterion for Chinese herbal compound so it has been a urgent problem to set up the quality control standards and reveal therapeutic basis of Chinese herbal compound.In order to give full play to the advantages of Chinese herbal compound,modern scientific and technological is used to research of Chinese herbal compound,especially the high performance liquid chromatography tandem mass spectrometry(HPLC-MS),because it is high sensitive,rapid,and obtain more information.It is very necessary that HPLC-MS is uesed to elucidate the effective components of basic substances of Chinese Herbal Compound,and endow traditional Chinese medicine with modern scientific connotation.
文摘建立高效液相色谱检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮3种化合物含量的方法,对比评价超声辅助提取法、回流提取法、微波辅助提取法、酶解提取法4种不同提取方法对桦褐孔菌粗提物中活性成分含量的影响。结果表明,该检测方法精密度较高,3种化合物的精密度的相对标准偏差(relative standard deviation,RSD)分别为0.14%、0.06%、0.20%;重复性RSD分别为1.67%、1.98%、2.22%;在24 h内稳定性较好,其RSD分别为0.20%、0.61%、0.25%。对比不同提取方法对3种化合物的影响,发现采用超声辅助提取法得到的目标化合物产量较高,适用于桦褐孔菌中主要活性成分的提取。该研究建立的HPLC法检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮含量的结果准确。
文摘Lemon oils are broadly used as flavoring agents in beverages,foods,cosmetics and pharmaceuticals,yet the adulteration of natural,particularly cold pressed lemon oils is very common in the industry due to its unmet demand and high cost.Nowadays,most quality control(QC)analysis of lemon oils is conducted by gas chromatography(GC)analysis,which is far from a reliable method.Oxygen heterocyclic compounds(OHCs)in non-volatile fraction are gaining increasing attention in authentication process because of the nearly finger-printing profiles of OHCs in cold pressed citrus essential oils.Our goal in this study was to identify OHCs using high performance liquid chromatography(HPLC)in lemon oils,establish OHC profiles,perform stepwise logistic regression analysis(SLRA)and build effective predicting model and further determine adulterated lemon oils by referencing the OHC profiles and established models.After HPLC analyses,profiling and SLRA modeling of 154 OHCs samples of industrial lemon oils,we found that the combination of isopimpinellin and total OHC concentration are essential and robust predictors to differentiate authentic samples from adulterated lemon oils with a success rate of 98%from the 5-fold cross validation.This study provided a reliable and efficient method in determining the authenticity of lemon oils.
