Expression of proliferating cell nuclear antigen (PCNA) in human colorectal lesions was studied immunohistologically and compared with the results of silver stained nucleolar organized regions (AgNOR)counting for eval...Expression of proliferating cell nuclear antigen (PCNA) in human colorectal lesions was studied immunohistologically and compared with the results of silver stained nucleolar organized regions (AgNOR)counting for evaluating the changes of proliferative kinetics in adenoma-carcinoma sequence of the large intestine.Ninety-six formalin-fixed paraffin-embedded specimens were used, which consisted of 21 normal colon mucosa,23 adenocarcinomas and 52 adenomas (34 cases with mild atypia, 12 morderate and 6 severe). Three types of PCNA distribution pattern were identified. 73. 9% normal mucosa was type A pattern. Type C pattern was characteristic of adenocarcinoma, and adenoma was of type B or C pattern. Labeling index (LI) of PCNA was an important parameter to evaluate the proliferation activity. In normal mucosa, LI of PCNA was 0. 34±0. 10, while in malignant lesions, it was 0. 71±0. 09. Furthermore, the LI had a tendency to elevate with the degree of atypia in adenoma from 0. 52±0. 10 in mild atypia to 0. 68±0. 07 in severe atypia. The pattern of AgNOR counting changes, which were 1. 97±0. 36 and 4. 16±1. 24 in the normal mucosa and malignant mucosa respectively, was found to be similar to Ll changes of the PCNA in different lesions of the same section. A good correlation was found between the mean number of AgNOR per nucleus and the LI of PCNA in 23 adenocarcinomas (r=0. 59, P <0. 05). These results suggested that proliferation kinetics could be comprehensively evaluated by combining immunohistological staining for PCNA and a simple silver staining for NOR, which would be advantageous for the diagnosis of colorectal lesions and for evaluating the degree of histopathological atypia of adenomas.展开更多
Objective：To evaluate the effects of curcumin on regulating the proliferation,cell cycle distribution,apoptosis and relevant mechanisms in keratinocyte cell lines.Methods：The human immortalized human keratinocyte li...Objective：To evaluate the effects of curcumin on regulating the proliferation,cell cycle distribution,apoptosis and relevant mechanisms in keratinocyte cell lines.Methods：The human immortalized human keratinocyte lines（HaCaT cells） were treated with different doses of curcumin.The effects of curcumin on cell viability were measured by MTT assay,and the cell cycle distribution and apoptosis determined by flow cytometry.The mRNA expression changes of proliferating cell nuclear antigen（PCNA）,cyclin D1 and Bcl-xL were from real-time PCR analysis and the protein levels were detected by Western blotting.Results：Data obtained in the study showed that curcumin could cause significantly inhibitory effect on proliferation in HaCaT cells in a time- and dose-dependent manner.Cell arrest at G1/S phase and significant apoptosis were observed after being treated with curcumin for 24 h.In association with these,the expression of PCNA,cyclin D1 and Bcl-xL were decreased both at mRNA and protein levels for the same treatment.Conclusion：Curcumin can inhibit proliferation,induce cell arrest at G1/S phase and cause apoptosis in HaCaT cells.The decreased expression of PCNA,cyclin D1 and Bcl-xL induced by curcumin contributes to the above effects in vitro.展开更多
文摘Expression of proliferating cell nuclear antigen (PCNA) in human colorectal lesions was studied immunohistologically and compared with the results of silver stained nucleolar organized regions (AgNOR)counting for evaluating the changes of proliferative kinetics in adenoma-carcinoma sequence of the large intestine.Ninety-six formalin-fixed paraffin-embedded specimens were used, which consisted of 21 normal colon mucosa,23 adenocarcinomas and 52 adenomas (34 cases with mild atypia, 12 morderate and 6 severe). Three types of PCNA distribution pattern were identified. 73. 9% normal mucosa was type A pattern. Type C pattern was characteristic of adenocarcinoma, and adenoma was of type B or C pattern. Labeling index (LI) of PCNA was an important parameter to evaluate the proliferation activity. In normal mucosa, LI of PCNA was 0. 34±0. 10, while in malignant lesions, it was 0. 71±0. 09. Furthermore, the LI had a tendency to elevate with the degree of atypia in adenoma from 0. 52±0. 10 in mild atypia to 0. 68±0. 07 in severe atypia. The pattern of AgNOR counting changes, which were 1. 97±0. 36 and 4. 16±1. 24 in the normal mucosa and malignant mucosa respectively, was found to be similar to Ll changes of the PCNA in different lesions of the same section. A good correlation was found between the mean number of AgNOR per nucleus and the LI of PCNA in 23 adenocarcinomas (r=0. 59, P <0. 05). These results suggested that proliferation kinetics could be comprehensively evaluated by combining immunohistological staining for PCNA and a simple silver staining for NOR, which would be advantageous for the diagnosis of colorectal lesions and for evaluating the degree of histopathological atypia of adenomas.
文摘Objective：To evaluate the effects of curcumin on regulating the proliferation,cell cycle distribution,apoptosis and relevant mechanisms in keratinocyte cell lines.Methods：The human immortalized human keratinocyte lines（HaCaT cells） were treated with different doses of curcumin.The effects of curcumin on cell viability were measured by MTT assay,and the cell cycle distribution and apoptosis determined by flow cytometry.The mRNA expression changes of proliferating cell nuclear antigen（PCNA）,cyclin D1 and Bcl-xL were from real-time PCR analysis and the protein levels were detected by Western blotting.Results：Data obtained in the study showed that curcumin could cause significantly inhibitory effect on proliferation in HaCaT cells in a time- and dose-dependent manner.Cell arrest at G1/S phase and significant apoptosis were observed after being treated with curcumin for 24 h.In association with these,the expression of PCNA,cyclin D1 and Bcl-xL were decreased both at mRNA and protein levels for the same treatment.Conclusion：Curcumin can inhibit proliferation,induce cell arrest at G1/S phase and cause apoptosis in HaCaT cells.The decreased expression of PCNA,cyclin D1 and Bcl-xL induced by curcumin contributes to the above effects in vitro.
