Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the ...Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.展开更多
Fasciclin-like arabinogalactan proteins(FLAs),a subclass of arabinogalactan proteins(AGPs),are usually involved in cell development in plants.To investigate the expression profiling as well
Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls...Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls and which could play a role in controlling early fiber development.Chief among these was GhMYB25,a Mixta-like MYB gene.Transgenic GhMYB25-silenced cotton展开更多
In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed
The effect of signal sampling depth in SIMS on profile was not reported We have found that the maximum sampling depth in SIMS is considerably bigger than the mean penetration range of primary ions and can skew the pro...The effect of signal sampling depth in SIMS on profile was not reported We have found that the maximum sampling depth in SIMS is considerably bigger than the mean penetration range of primary ions and can skew the profile of secondary ion counts for implanted samples on the more deeper direction from the surface. The effect of maximum sampling depth is true not only for implanted samples but also for the samples in the middle of which there is an other impurity-rich layer. The action principle of signal sampling depth effect and the method of decreasing the error produced by the effect are discussed in this展开更多
To extend our successful Magnetotelluric(MT) experiments in the area from Yadong to Bamocuo in 1995 (Chen et al., 1996), two new Magnetotelluric(MT)experiments have been carried out along two main profiles in the cent...To extend our successful Magnetotelluric(MT) experiments in the area from Yadong to Bamocuo in 1995 (Chen et al., 1996), two new Magnetotelluric(MT)experiments have been carried out along two main profiles in the central and Northern Tibetan Plateau in the summer of 1998 and 1999. While the 1995 MT work mainly focused on the study of the electrical structure of the Yarlung\|Zangbo River Suture , the 1998 and 1999 experiments have been designed with following purpose:(1) Study whether partially molten layer widely exists in the crust of the central and northern Tibet.(2) Study the electrical structures of crust and upper mantle in central and northern Tibet which may relate to the attenuated Shear Seismic waves.(3) Study the detail electrical structures of Bangong\|Nujiang Suture and Jinsha Suture.In 1998, the MT team (China University of Geosciences, University of Washington and Geological Survey of Canada) recorded MT data along the Tibet 500 Line which extends about three hundred and eighty kilometers from Deqing to Longweicuo. We used LIMS system to record the long period(20～20000s) MT data at twenty\|six sites and used MT24 to record broadband(320Hz,2000s) MT data at fifty\|eight sites.展开更多
The first troposphere wind profiling radar in China has been in operation. The paper describes the radar parameters and characteristics with some experimental results presented.
Traditional manufacturing processes for lightweight curved profiles are often associated with lengthy procedures,high costs,low efficiency,and high energy consumption.In order to solve this problem,a new staggered ext...Traditional manufacturing processes for lightweight curved profiles are often associated with lengthy procedures,high costs,low efficiency,and high energy consumption.In order to solve this problem,a new staggered extrusion(SE)process was used to form the curved profile of AZ31 magnesium alloy in this paper.The study investigates the mapping relationship between the curvature,microstructure,and mechanical properties of the formed profiles by using different eccentricities of the die.Scanning electron microscopy(SEM)and electron backscatter diffraction techniques are employed to examine the effects of different eccentricity values(e)on grain morphology,recrystallization mechanisms,texture,and Schmid factors of the products.The results demonstrate that the staggered extrusion method promotes the deep refinement of grain size in the extruded products,with an average grain size of only 15%of the original billet,reaching 12.28μm.The tensile strength and elongation of the curved profiles after extrusion under the eccentricity value of 10 mm,20 mm and 30 mm are significantly higher than those of the billet,with the tensile strength is increased to 250,270,235 MPa,and the engineering strain elongation increased to 10.5%,12.1%,15.9%.This indicates that staggered extrusion enables curvature control of the profiles while improving their strength.展开更多
With the arrival of the big data era,the phenomenon of information overload is becoming increasingly severe.In response to the common issue of sparse user rating matrices in recommendation systems,a collaborative filt...With the arrival of the big data era,the phenomenon of information overload is becoming increasingly severe.In response to the common issue of sparse user rating matrices in recommendation systems,a collaborative filtering recommendation algorithm was proposed based on improved user profiles in this study.Firstly,a profile labeling system was constructed based on user characteristics.This study proposed that user profile labels should be created using basic user information and basic item information,in order to construct multidimensional user profiles.TF-IDF algorithm was used to determine the weights of user-item feature labels.Secondly,user similarity was calculated by weighting both profile-based collaborative filtering and user-based collaborative filtering algorithms,and the final user similarity was obtained by harmonizing these weights.Finally,personalized recommendations were generated using Top-N method.Validation with the MovieLens-1M dataset revealed that this algorithm enhances both recommendation Precision and Recall compared to single-method approaches(recommendation algorithm based on user portrait and user-based collaborative filtering algorithm).展开更多
In the data-driven era of the internet and business environments,constructing accurate user profiles is paramount for personalized user understanding and classification.The traditional TF-IDF algorithm has some limita...In the data-driven era of the internet and business environments,constructing accurate user profiles is paramount for personalized user understanding and classification.The traditional TF-IDF algorithm has some limitations when evaluating the impact of words on classification results.Consequently,an improved TF-IDF-K algorithm was introduced in this study,which included an equalization factor,aimed at constructing user profiles by processing and analyzing user search records.Through the training and prediction capabilities of a Support Vector Machine(SVM),it enabled the prediction of user demographic attributes.The experimental results demonstrated that the TF-IDF-K algorithm has achieved a significant improvement in classification accuracy and reliability.展开更多
文摘Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.
文摘Fasciclin-like arabinogalactan proteins(FLAs),a subclass of arabinogalactan proteins(AGPs),are usually involved in cell development in plants.To investigate the expression profiling as well
文摘Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls and which could play a role in controlling early fiber development.Chief among these was GhMYB25,a Mixta-like MYB gene.Transgenic GhMYB25-silenced cotton
文摘In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed
基金This work is supported by National Natural Science Fundation.
文摘The effect of signal sampling depth in SIMS on profile was not reported We have found that the maximum sampling depth in SIMS is considerably bigger than the mean penetration range of primary ions and can skew the profile of secondary ion counts for implanted samples on the more deeper direction from the surface. The effect of maximum sampling depth is true not only for implanted samples but also for the samples in the middle of which there is an other impurity-rich layer. The action principle of signal sampling depth effect and the method of decreasing the error produced by the effect are discussed in this
文摘To extend our successful Magnetotelluric(MT) experiments in the area from Yadong to Bamocuo in 1995 (Chen et al., 1996), two new Magnetotelluric(MT)experiments have been carried out along two main profiles in the central and Northern Tibetan Plateau in the summer of 1998 and 1999. While the 1995 MT work mainly focused on the study of the electrical structure of the Yarlung\|Zangbo River Suture , the 1998 and 1999 experiments have been designed with following purpose:(1) Study whether partially molten layer widely exists in the crust of the central and northern Tibet.(2) Study the electrical structures of crust and upper mantle in central and northern Tibet which may relate to the attenuated Shear Seismic waves.(3) Study the detail electrical structures of Bangong\|Nujiang Suture and Jinsha Suture.In 1998, the MT team (China University of Geosciences, University of Washington and Geological Survey of Canada) recorded MT data along the Tibet 500 Line which extends about three hundred and eighty kilometers from Deqing to Longweicuo. We used LIMS system to record the long period(20～20000s) MT data at twenty\|six sites and used MT24 to record broadband(320Hz,2000s) MT data at fifty\|eight sites.
文摘The first troposphere wind profiling radar in China has been in operation. The paper describes the radar parameters and characteristics with some experimental results presented.
基金Project(JQ2022E004)supported by the Natural Science Foundation of Heilongjiang Province,China。
文摘Traditional manufacturing processes for lightweight curved profiles are often associated with lengthy procedures,high costs,low efficiency,and high energy consumption.In order to solve this problem,a new staggered extrusion(SE)process was used to form the curved profile of AZ31 magnesium alloy in this paper.The study investigates the mapping relationship between the curvature,microstructure,and mechanical properties of the formed profiles by using different eccentricities of the die.Scanning electron microscopy(SEM)and electron backscatter diffraction techniques are employed to examine the effects of different eccentricity values(e)on grain morphology,recrystallization mechanisms,texture,and Schmid factors of the products.The results demonstrate that the staggered extrusion method promotes the deep refinement of grain size in the extruded products,with an average grain size of only 15%of the original billet,reaching 12.28μm.The tensile strength and elongation of the curved profiles after extrusion under the eccentricity value of 10 mm,20 mm and 30 mm are significantly higher than those of the billet,with the tensile strength is increased to 250,270,235 MPa,and the engineering strain elongation increased to 10.5%,12.1%,15.9%.This indicates that staggered extrusion enables curvature control of the profiles while improving their strength.
文摘With the arrival of the big data era,the phenomenon of information overload is becoming increasingly severe.In response to the common issue of sparse user rating matrices in recommendation systems,a collaborative filtering recommendation algorithm was proposed based on improved user profiles in this study.Firstly,a profile labeling system was constructed based on user characteristics.This study proposed that user profile labels should be created using basic user information and basic item information,in order to construct multidimensional user profiles.TF-IDF algorithm was used to determine the weights of user-item feature labels.Secondly,user similarity was calculated by weighting both profile-based collaborative filtering and user-based collaborative filtering algorithms,and the final user similarity was obtained by harmonizing these weights.Finally,personalized recommendations were generated using Top-N method.Validation with the MovieLens-1M dataset revealed that this algorithm enhances both recommendation Precision and Recall compared to single-method approaches(recommendation algorithm based on user portrait and user-based collaborative filtering algorithm).
文摘In the data-driven era of the internet and business environments,constructing accurate user profiles is paramount for personalized user understanding and classification.The traditional TF-IDF algorithm has some limitations when evaluating the impact of words on classification results.Consequently,an improved TF-IDF-K algorithm was introduced in this study,which included an equalization factor,aimed at constructing user profiles by processing and analyzing user search records.Through the training and prediction capabilities of a Support Vector Machine(SVM),it enabled the prediction of user demographic attributes.The experimental results demonstrated that the TF-IDF-K algorithm has achieved a significant improvement in classification accuracy and reliability.
