BACKGROUND: Triggering receptor expressed on myeloid cells-1(TREM-1) is a cell surface receptor expressed on neutrophils and monocytes. TREM-1 acts to amplify infl ammation and serves as a critical mediator of infl am...BACKGROUND: Triggering receptor expressed on myeloid cells-1(TREM-1) is a cell surface receptor expressed on neutrophils and monocytes. TREM-1 acts to amplify infl ammation and serves as a critical mediator of infl ammatory response in the context of sepsis. To date, the predisposition of TREM-1 gene polymorphisms to septic shock has not been reported. This study was designed to investigate whether TREM-1 genomic variations are associated with the development of septic shock.METHODS: We genotyped two TREM-1 single nucleotide polymorphisms(SNPs, rs2234237 and rs2234246) and evaluated the relationships between these SNPs and septic shock on susceptibility and prognosis.RESULTS: TREM-1 rs2234246 A allele in the promoter region was signifi cantly associated with the susceptibility of septic shock in recessive model(AA, OR=3.10, 95%CI 1.15 to 8.32, P=0.02), and in codominant model(AG, OR=0.72, 95%CI 0.43–1.19, P=0.02; AA, OR=2.71, 95%CI 1.00–7.42; P=0.03). However, in three inherited models(dominant model, recessive model, and codominant model), none of the assayed loci was signif icantly associated with the prognosis of septic shock. The nonsurvivor group demonstrated higher plasma IL-6 levels(99.7±34.7 pg/mL vs. 61.2±26.5 pg/mL, P<0.01) than the survivor group. Plasma concentrations of IL-6 among the three genotypes of rs2234246 were AA 99.4±48.9 pg/m L, AG 85.4±43 pg/m L, and GG 65.3±30.7 pg/m L(P<0.01). The plasma concentrations of IL-6 in patients with AA genotypes were signifi cantly higher than those in patients with GG genotypes(P<0.01).CONCLUSION: TREM-1 genetic polymorphisms rs2234246 may be significantly correlated only with susceptibility to septic shock in the Chinese Han population.展开更多
RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holo...RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.展开更多
Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms i...Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms in Zhejiang Province,China,were investigated.A total of 215 isolates were identified as Escherichia coli(64.65%),Klebsiella pneumoniae(12.09%),Proteus mirabilis(10.23%),Salmonella(8.84%),and Enterobacter cloacae(4.19%).Meanwhile,all isolates were resistant to at least two antibiotics.Most isolates carried tet(A)(85.12%),blaTEM(78.60%)and sul1(67.44%)resistance genes.Gene co-occurrence analysis showed that the resistance genes were associated with IS26 and integrons.A conjugative IncFII plasmid pSDM004 containing all the above MGEs was detected in Proteus mirabilis isolate SDM004.This isolate was resistant to 18 antibiotics and carried the blaNDM-5 gene.MGEs,especially plasmids,are the primary antibiotic resistance gene transmission route in duck farms.These findings provide a theoretical basis for the rational use of antibiotics in farms which are substantial for evaluating public health and food safety.展开更多
Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSP...Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.展开更多
Reducing the vulnerability of a platform,i.e.,the risk of being affected by hostile objects,is of paramount importance in the design process of vehicles,especially aircraft.A simple and effective way to decrease vulne...Reducing the vulnerability of a platform,i.e.,the risk of being affected by hostile objects,is of paramount importance in the design process of vehicles,especially aircraft.A simple and effective way to decrease vulnerability is to introduce protective structures to intercept and possibly stop threats.However,this type of solution can lead to a significant increase in weight,affecting the performance of the aircraft.For this reason,it is crucial to study possible solutions that allow reducing the vulnerability of the aircraft while containing the increase in structural weight.One possible strategy is to optimize the topology of protective solutions to find the optimal balance between vulnerability and the weight of the added structures.Among the many optimization techniques available in the literature for this purpose,multiobjective genetic algorithms stand out as promising tools.In this context,this work proposes the use of a in-house software for vulnerability calculation to guide the process of topology optimization through multi-objective genetic algorithms,aiming to simultaneously minimize the weight of protective structures and vulnerability.In addition to the use of the in-house software,which itself represents a novelty in the field of topology optimization of structures,the method incorporates a custom mutation function within the genetic algorithm,specifically developed using a graph-based approach to ensure the continuity of the generated structures.The tool developed for this work is capable of generating protections with optimized layouts considering two different types of impacting objects,namely bullets and fragments from detonating objects.The software outputs a set of non-dominated solutions describing different topologies that the user can choose from.展开更多
Currently,deep drilling operates under extreme conditions of high temperature and high pressure,demanding more from subterranean power motors.The all-metal positive displacement motor,known for its robust performance,...Currently,deep drilling operates under extreme conditions of high temperature and high pressure,demanding more from subterranean power motors.The all-metal positive displacement motor,known for its robust performance,is a critical choice for such drilling.The dimensions of the PDM are crucial for its performance output.To enhance this,optimization of the motor's profile using a genetic algorithm has been undertaken.The design process begins with the computation of the initial stator and rotor curves based on the equations for a screw cycloid.These curves are then refined using the least squares method for a precise fit.Following this,the PDM's mathematical model is optimized,and motor friction is assessed.The genetic algorithm process involves encoding variations and managing crossovers to optimize objective functions,including the isometric radius coefficient,eccentricity distance parameter,overflow area,and maximum slip speed.This optimization yields the ideal profile parameters that enhance the motor's output.Comparative analyses of the initial and optimized output characteristics were conducted,focusing on the effects of the isometric radius coefficient and overflow area on the motor's performance.Results indicate that the optimized motor's overflow area increased by 6.9%,while its rotational speed reduced by 6.58%.The torque,as tested by Infocus,saw substantial improvements of38.8%.This optimization provides a theoretical foundation for improving the output characteristics of allmetal PDMs and supports the ongoing development and research of PDM technology.展开更多
The global prevalence rate for congenital hydrocephalus(CH)is approximately one out of every five hundred births with multifaceted predisposing factors at play.Genetic influences stand as a major contributor to CH pat...The global prevalence rate for congenital hydrocephalus(CH)is approximately one out of every five hundred births with multifaceted predisposing factors at play.Genetic influences stand as a major contributor to CH pathogenesis,and epidemiological evidence suggests their involvement in up to 40%of all cases observed globally.Knowledge about an individual’s genetic susceptibility can significantly improve prognostic precision while aiding clinical decision-making processes.However,the precise genetic etiology has only been pinpointed in fewer than 5%of human instances.More occurrences of CH cases are required for comprehensive gene sequencing aimed at uncovering additional potential genetic loci.A deeper comprehension of its underlying genetics may offer invaluable insights into the molecular and cellular basis of this brain disorder.This review provides a summary of pertinent genes identified through gene sequencing technologies in humans,in addition to the 4 genes currently associated with CH(2 X-linked genes L1CAM and AP1S2,2 autosomal recessive MPDZ and CCDC88C).Others predominantly participate in aqueduct abnormalities,ciliary movement,and nervous system development.The prospective CH-related genes revealed through animal model gene-editing techniques are further outlined,focusing mainly on 4 pathways,namely cilia synthesis and movement,ion channels and transportation,Reissner’s fiber(RF)synthesis,cell apoptosis,and neurogenesis.Notably,the proper functioning of motile cilia provides significant impulsion for cerebrospinal fluid(CSF)circulation within the brain ventricles while mutations in cilia-related genes constitute a primary cause underlying this condition.So far,only a limited number of CH-associated genes have been identified in humans.The integration of genotype and phenotype for disease diagnosis represents a new trend in the medical field.Animal models provide insights into the pathogenesis of CH and contribute to our understanding of its association with related complications,such as renal cysts,scoliosis,and cardiomyopathy,as these genes may also play a role in the development of these diseases.Genes discovered in animals present potential targets for new treatments but require further validation through future human studies.展开更多
Magnetic field design is essential for the operation of Hall thrusters.This study focuses on utilizing a genetic algorithm to optimize the magnetic field configuration of SPT70.A 2D hybrid PIC-DSMC and channel-wall er...Magnetic field design is essential for the operation of Hall thrusters.This study focuses on utilizing a genetic algorithm to optimize the magnetic field configuration of SPT70.A 2D hybrid PIC-DSMC and channel-wall erosion model are employed to analyze the plume divergence angle and wall erosion rate,while a Farady probe measurement and laser profilometry system are set up to verify the simulation results.The results demonstrate that the genetic algorithm contributes to reducing the divergence angle of the thruster plumes and alleviating the impact of high-energy particles on the discharge channel wall,reducing the erosion by 5.5%and 2.7%,respectively.Further analysis indicates that the change from a divergent magnetic field to a convergent magnetic field,combined with the upstream shift of the ionization region,contributes to the improving the operation of the Hall thruster.展开更多
The Random Amplified Polymorphism DNA (RAPD) markers were used to stu dy the intra-specific diversity and regional differentiation of the Chinese fore st frogs (Rana chensinensis), which were sampled from the fields o...The Random Amplified Polymorphism DNA (RAPD) markers were used to stu dy the intra-specific diversity and regional differentiation of the Chinese fore st frogs (Rana chensinensis), which were sampled from the fields of 8 regions in Heilongjiang Province. Totally 78 polymorphic DNA loci were amplified by 10 RAP D primers. By genetic distance analysis and phylogenetic tree reconstruction wit h the Neighbor-Joining (NJ) method the results showed that the populations of Ch inese forest frogs distributed in 8 regions existed great differentiation (Avera ge Fst=0.347, SD=0.235) while there was the paradox between geographic distances and genetic distances. Based on geographic and geological data, a hypothesis wa s posed that it is very possible that the hilly lands in the downstream of the S onghua River and the Heilong River were the center of the origin of the Chinese forest frogs in Heilongjiang Province. And mainly through the Songhua River syst em, the Chinese forest frogs dispersed into the Songneng Plain from the Sanjiang Plain whereas the Fangzheng region became a sub-center for the western dispersi on.展开更多
The performance of genetic algorithm(GA) is determined by the capability of search and optimization for satisfactory solutions. The new adaptive genetic algorithm(AGA) is built for inducing suitable search and optimiz...The performance of genetic algorithm(GA) is determined by the capability of search and optimization for satisfactory solutions. The new adaptive genetic algorithm(AGA) is built for inducing suitable search and optimization relationship. The use of six fuzzy logic controllers(6FLCs) is proposed for dynamic control genetic operating parameters of a symbolic-coded GA. This paper uses AGA based on 6FLCs to deal with the travelling salesman problem (TSP). Experimental results show that AGA based on 6FLCs is more efficient than a standard GA in solving combinatorial optimization problems similar to TSP.展开更多
In the flexible job-shop scheduling problem （FJSP）, each operation has to be assigned to a machine from a set of capable machines before alocating the assigned operations on all machines. To solve the multi-objectiv...In the flexible job-shop scheduling problem （FJSP）, each operation has to be assigned to a machine from a set of capable machines before alocating the assigned operations on all machines. To solve the multi-objective FJSP, the Grantt graph oriented string representation （GOSR） and the basic manipulation of the genetic algorithm operator are presented. An integrated operator genetic algorithm （IOGA） and its process are described. Comparison between computational results and the latest research shows that the proposed algorithm is effective in reducing the total workload of all machines, the makespan and the critical machine workload.展开更多
The history of medical genetics is briefly reviewed. It is evident that medical genetics with its inseparable part, clinical genetics, started out as a cfinical science from the very beginning. Its robust development ...The history of medical genetics is briefly reviewed. It is evident that medical genetics with its inseparable part, clinical genetics, started out as a cfinical science from the very beginning. Its robust development in the developed countries is the result of a close interaction between the basic sciences and clinical genetics. In China, however, clinical genetics has not received due emphasis and medical genetics is still not recognized as one of the medical specialties. This is in marked contrast to the situation in the West. It is high time to acknowledge that medical genetics is a medical specialty and to promote clinical genetics service in qualified hospitals in our country.展开更多
Genetic diversity and genetic differentiation of natural populations of Pinus kesiya var. langbinanensis were examined by means of electrophoresis technique. Analysis of 9 enzyme systems including 16 loci showed that ...Genetic diversity and genetic differentiation of natural populations of Pinus kesiya var. langbinanensis were examined by means of electrophoresis technique. Analysis of 9 enzyme systems including 16 loci showed that all the three natural popu-lations of the pine were high in genetic diversity but low in inter-population genetic differentiation. The proportion of polymorphic loci is 0.667, with each locus holding 2.13 alleles, averagely. The average expected and observed heterozygosity was 0.288 and 0.197, respectively. The gene differentiation among populations was 0.052, but the mean genetic distance was only 0.015.展开更多
Polymorphism of nine strains （CF05, CF09, 29, 916, AU9, Chang10, Chang7, 8808 and AU. Japanese） of A. auricular cultivated in Heilongjiang Province were analyzed by RAPD （Random Amplication polymorphic DNA）. Thirt...Polymorphism of nine strains （CF05, CF09, 29, 916, AU9, Chang10, Chang7, 8808 and AU. Japanese） of A. auricular cultivated in Heilongjiang Province were analyzed by RAPD （Random Amplication polymorphic DNA）. Thirteen primers were selected from forty PCR primers with 10bp long random primer. The results showed that nine strains of A. auricular have a high level of genetic diversity and the percentage of DNA polymorphic was 96.05. The genotypes of 9 strains of Auricularia auricular were identified by the fingerprints from primer 27 and primer 46 by RAPD analysis. The results are helpful for quickly identifying strains of A. auricular in its early breeding time, and also provides a powerful theoretic basis to differentiate strains （Auricularia auricular） whose morphology is very similar in breeding programs of edible fungus.展开更多
Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many use...Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many uses, easiness of tissue culture and small gene group that make them well suited as a model system for the application of genetic engineering in forest trees. In the last decade, much progress has been made in genetic improvement of poplar species in China. Modern biotechnology is an important tool for genetic improvement in forest trees, and its applications to genetic improvement in poplars, which covers genetic transformation, gene expression, construction of genetic linkage map, QTLs (quantitative trait loci) identification and molecular assisted selection are reviewed in this paper. At the same time, the existing problems and outlook about the application of modern biotechnology to genetic improvement in forest trees are also discussed.展开更多
Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine ...Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine to cervids, a s well as a few loci derived directly from deer microsatellite library. These lo ci have been used in parentage determination, genetic diversity and population s tructure, population introgression, as genetic marker gestation length and winte ring survival et al. However, microsatellite loci presently found are untouchabl e to the demand of application. Future work should include: 1) isolating a large number of cervine microsatellite loci, 2) constructing genetic and physical map s of microsatellite loci. So that microsatelites have a strong base for advanced applications in deer.展开更多
Proper fixture design is crucial to obtain the better product quality according to the design specification during the workpiece fabrication. Locator layout planning is one of the most important tasks in the fixture ...Proper fixture design is crucial to obtain the better product quality according to the design specification during the workpiece fabrication. Locator layout planning is one of the most important tasks in the fixture design process. However, the design of a fixture relies heavily on the designerts expertise and experience up to now. Therefore, a new approach to loeator layout determination for workpieces with arbitrary complex surfaces is pro- posed for the first time. Firstly, based on the fuzzy judgment method, the proper locating reference and locator - numbers are determined with consideration of surface type, surface area and position tolerance. Secondly, the lo- cator positions are optimized by genetic algorithm（GA）. Finally, a typical example shows that the approach is su- perior to the experiential method and can improve positioning accuracy effectively.展开更多
Two dimensional(2 D) entropy method has to pay the price of time when applied to image segmentation. So the genetic algorithm is introduced to improve the computational efficiency of the 2 D entropy method. The pro...Two dimensional(2 D) entropy method has to pay the price of time when applied to image segmentation. So the genetic algorithm is introduced to improve the computational efficiency of the 2 D entropy method. The proposed method uses both the gray value of a pixel and the local average gray value of an image. At the same time, the simple genetic algorithm is improved by using better reproduction and crossover operators. Thus the proposed method makes up the 2 D entropy method’s drawback of being time consuming, and yields satisfactory segmentation results. Experimental results show that the proposed method can save computational time when it provides good quality segmentation.展开更多
With completion of the Populus genome sequencing project and the availability of many expressed sequence tags （ESTs） databases in forest trees, attention is now rapidly shifting towards the study of individual genet...With completion of the Populus genome sequencing project and the availability of many expressed sequence tags （ESTs） databases in forest trees, attention is now rapidly shifting towards the study of individual genetic variation in natural populations. The most abundant form of genetic variation in many eukaryotic species is represented by single nucleotide polymorphisms （SNPs）, which can account for heritable inter-individual differences in complex phenotypes. Unlike humans, the linkage disequilibrium （LD） rapidly decays within candidate genes in forest trees. Thus, SNPs-based candidate gene association studies are considered to be a most effective approach to dissect the complex quantitative traits in forest trees. The present study demonstrates that LD mapping can be used to identify alleles associated with quantitative traits and suggests that this new approach could be particularly useful for performing breeding programs in forest trees. In this review, we will describe the fundamentals, patterns of SNPs distribution and frequency, summarize recent advances in SNPs discovery and LD and comment on the application of LD in the dissection of complex quantitative traits in forest tress. We also put forward the outlook for future SNPs-based association analysis of quantitative traits in forest trees.展开更多
BACKGROUND： The tumor necrosis factor recepter associated factor （TRAF） 6 is an important intracellular adapter protein that plays a pivotal role in activating multiple inflammatory and immune related processes ind...BACKGROUND： The tumor necrosis factor recepter associated factor （TRAF） 6 is an important intracellular adapter protein that plays a pivotal role in activating multiple inflammatory and immune related processes induced by cytokines. TRAF6 represents a strong candidate susceptibility factor for sepsis. We investigated whether polymorphisms at the TRAF6 gene are associated with the susceptibility to and severity of sepsis.METHODS： A hospital-based case-control study was conducted with 255 patients with sepsis and 260 controls who were recruited from Zhengzhou, China. Haplotype tagging single nucleotide polymorphisms （htSNPs） were selected from the HapMap database and genotyped using the SNPstream genotyping platform. The associations with the susceptibility and disease severity of sepsis were estimated by logistic regression, and adjusted for age, sex, smoking, drinking, chronic diseases status, APACHEII score and critical illness status.RESULTS： A total of 13 TRAF6 SNPs were tagged by 7 htSNPs. Five htSNPs （rs5030490, rs5030411, rs5030416, rs5030445 and rs3740961） were genotyped in the case control study. Genotype frequencies of the htSNPs were conformed to the Hardy-Weinberg equilibrium in both patients and controls. No significant association was found between the 5 htSNPs and the susceptibility to and severity of sepsis. Compared with the main haplotype -11120A/-10688T/-9423A/805G/12967G, no certain haplotype was associated with the signi？ cantly susceptibility to or severity of sepsis.CONCLUSION： TRAF6 gene polymorphisms might not play a major role in mediating the susceptibility to and severity of sepsis in the Chinese population. A larger population-based case-control study is warranted.展开更多
基金supported by Science&Technology Pillar Program of Guangdong Province(2009BAI86B03)
文摘BACKGROUND: Triggering receptor expressed on myeloid cells-1(TREM-1) is a cell surface receptor expressed on neutrophils and monocytes. TREM-1 acts to amplify infl ammation and serves as a critical mediator of infl ammatory response in the context of sepsis. To date, the predisposition of TREM-1 gene polymorphisms to septic shock has not been reported. This study was designed to investigate whether TREM-1 genomic variations are associated with the development of septic shock.METHODS: We genotyped two TREM-1 single nucleotide polymorphisms(SNPs, rs2234237 and rs2234246) and evaluated the relationships between these SNPs and septic shock on susceptibility and prognosis.RESULTS: TREM-1 rs2234246 A allele in the promoter region was signifi cantly associated with the susceptibility of septic shock in recessive model(AA, OR=3.10, 95%CI 1.15 to 8.32, P=0.02), and in codominant model(AG, OR=0.72, 95%CI 0.43–1.19, P=0.02; AA, OR=2.71, 95%CI 1.00–7.42; P=0.03). However, in three inherited models(dominant model, recessive model, and codominant model), none of the assayed loci was signif icantly associated with the prognosis of septic shock. The nonsurvivor group demonstrated higher plasma IL-6 levels(99.7±34.7 pg/mL vs. 61.2±26.5 pg/mL, P<0.01) than the survivor group. Plasma concentrations of IL-6 among the three genotypes of rs2234246 were AA 99.4±48.9 pg/m L, AG 85.4±43 pg/m L, and GG 65.3±30.7 pg/m L(P<0.01). The plasma concentrations of IL-6 in patients with AA genotypes were signifi cantly higher than those in patients with GG genotypes(P<0.01).CONCLUSION: TREM-1 genetic polymorphisms rs2234246 may be significantly correlated only with susceptibility to septic shock in the Chinese Han population.
基金supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry and Fisheries(IPET)through the AgriBioindustry Technology Development Programfunded by the Ministry of Agriculture,Food and Rural Affairs(MAFRA)(No.314009-3)
文摘RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.
基金supported by the National Natural Science Foundation of China(32172188)Science and Technology Cooperation Project of ZheJiang Province(2023SNJF058-3)。
文摘Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms in Zhejiang Province,China,were investigated.A total of 215 isolates were identified as Escherichia coli(64.65%),Klebsiella pneumoniae(12.09%),Proteus mirabilis(10.23%),Salmonella(8.84%),and Enterobacter cloacae(4.19%).Meanwhile,all isolates were resistant to at least two antibiotics.Most isolates carried tet(A)(85.12%),blaTEM(78.60%)and sul1(67.44%)resistance genes.Gene co-occurrence analysis showed that the resistance genes were associated with IS26 and integrons.A conjugative IncFII plasmid pSDM004 containing all the above MGEs was detected in Proteus mirabilis isolate SDM004.This isolate was resistant to 18 antibiotics and carried the blaNDM-5 gene.MGEs,especially plasmids,are the primary antibiotic resistance gene transmission route in duck farms.These findings provide a theoretical basis for the rational use of antibiotics in farms which are substantial for evaluating public health and food safety.
基金supported by the Scientific and Innovative Action Plan of Shanghai(21N31900800)Shanghai Rising-Star Program(23QB1403500)+4 种基金the Shanghai Sailing Program(20YF1443000)Shanghai Science and Technology Commission,the Belt and Road Project(20310750500)Talent Project of SAAS(2023-2025)Runup Plan of SAAS(ZP22211)the SAAS Program for Excellent Research Team(2022(B-16))。
文摘Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.
文摘Reducing the vulnerability of a platform,i.e.,the risk of being affected by hostile objects,is of paramount importance in the design process of vehicles,especially aircraft.A simple and effective way to decrease vulnerability is to introduce protective structures to intercept and possibly stop threats.However,this type of solution can lead to a significant increase in weight,affecting the performance of the aircraft.For this reason,it is crucial to study possible solutions that allow reducing the vulnerability of the aircraft while containing the increase in structural weight.One possible strategy is to optimize the topology of protective solutions to find the optimal balance between vulnerability and the weight of the added structures.Among the many optimization techniques available in the literature for this purpose,multiobjective genetic algorithms stand out as promising tools.In this context,this work proposes the use of a in-house software for vulnerability calculation to guide the process of topology optimization through multi-objective genetic algorithms,aiming to simultaneously minimize the weight of protective structures and vulnerability.In addition to the use of the in-house software,which itself represents a novelty in the field of topology optimization of structures,the method incorporates a custom mutation function within the genetic algorithm,specifically developed using a graph-based approach to ensure the continuity of the generated structures.The tool developed for this work is capable of generating protections with optimized layouts considering two different types of impacting objects,namely bullets and fragments from detonating objects.The software outputs a set of non-dominated solutions describing different topologies that the user can choose from.
基金supported by the National Natural Science Foundation of China (No.42172343)。
文摘Currently,deep drilling operates under extreme conditions of high temperature and high pressure,demanding more from subterranean power motors.The all-metal positive displacement motor,known for its robust performance,is a critical choice for such drilling.The dimensions of the PDM are crucial for its performance output.To enhance this,optimization of the motor's profile using a genetic algorithm has been undertaken.The design process begins with the computation of the initial stator and rotor curves based on the equations for a screw cycloid.These curves are then refined using the least squares method for a precise fit.Following this,the PDM's mathematical model is optimized,and motor friction is assessed.The genetic algorithm process involves encoding variations and managing crossovers to optimize objective functions,including the isometric radius coefficient,eccentricity distance parameter,overflow area,and maximum slip speed.This optimization yields the ideal profile parameters that enhance the motor's output.Comparative analyses of the initial and optimized output characteristics were conducted,focusing on the effects of the isometric radius coefficient and overflow area on the motor's performance.Results indicate that the optimized motor's overflow area increased by 6.9%,while its rotational speed reduced by 6.58%.The torque,as tested by Infocus,saw substantial improvements of38.8%.This optimization provides a theoretical foundation for improving the output characteristics of allmetal PDMs and supports the ongoing development and research of PDM technology.
基金supported by the National Key Research and Development Program of China(2021YFF1200602).
文摘The global prevalence rate for congenital hydrocephalus(CH)is approximately one out of every five hundred births with multifaceted predisposing factors at play.Genetic influences stand as a major contributor to CH pathogenesis,and epidemiological evidence suggests their involvement in up to 40%of all cases observed globally.Knowledge about an individual’s genetic susceptibility can significantly improve prognostic precision while aiding clinical decision-making processes.However,the precise genetic etiology has only been pinpointed in fewer than 5%of human instances.More occurrences of CH cases are required for comprehensive gene sequencing aimed at uncovering additional potential genetic loci.A deeper comprehension of its underlying genetics may offer invaluable insights into the molecular and cellular basis of this brain disorder.This review provides a summary of pertinent genes identified through gene sequencing technologies in humans,in addition to the 4 genes currently associated with CH(2 X-linked genes L1CAM and AP1S2,2 autosomal recessive MPDZ and CCDC88C).Others predominantly participate in aqueduct abnormalities,ciliary movement,and nervous system development.The prospective CH-related genes revealed through animal model gene-editing techniques are further outlined,focusing mainly on 4 pathways,namely cilia synthesis and movement,ion channels and transportation,Reissner’s fiber(RF)synthesis,cell apoptosis,and neurogenesis.Notably,the proper functioning of motile cilia provides significant impulsion for cerebrospinal fluid(CSF)circulation within the brain ventricles while mutations in cilia-related genes constitute a primary cause underlying this condition.So far,only a limited number of CH-associated genes have been identified in humans.The integration of genotype and phenotype for disease diagnosis represents a new trend in the medical field.Animal models provide insights into the pathogenesis of CH and contribute to our understanding of its association with related complications,such as renal cysts,scoliosis,and cardiomyopathy,as these genes may also play a role in the development of these diseases.Genes discovered in animals present potential targets for new treatments but require further validation through future human studies.
基金funded by Shanghai Natural Science Foundation(No.12ZR1414700)。
文摘Magnetic field design is essential for the operation of Hall thrusters.This study focuses on utilizing a genetic algorithm to optimize the magnetic field configuration of SPT70.A 2D hybrid PIC-DSMC and channel-wall erosion model are employed to analyze the plume divergence angle and wall erosion rate,while a Farady probe measurement and laser profilometry system are set up to verify the simulation results.The results demonstrate that the genetic algorithm contributes to reducing the divergence angle of the thruster plumes and alleviating the impact of high-energy particles on the discharge channel wall,reducing the erosion by 5.5%and 2.7%,respectively.Further analysis indicates that the change from a divergent magnetic field to a convergent magnetic field,combined with the upstream shift of the ionization region,contributes to the improving the operation of the Hall thruster.
基金the Natural Science Foundation of Heilongjiang Province (C9726).
文摘The Random Amplified Polymorphism DNA (RAPD) markers were used to stu dy the intra-specific diversity and regional differentiation of the Chinese fore st frogs (Rana chensinensis), which were sampled from the fields of 8 regions in Heilongjiang Province. Totally 78 polymorphic DNA loci were amplified by 10 RAP D primers. By genetic distance analysis and phylogenetic tree reconstruction wit h the Neighbor-Joining (NJ) method the results showed that the populations of Ch inese forest frogs distributed in 8 regions existed great differentiation (Avera ge Fst=0.347, SD=0.235) while there was the paradox between geographic distances and genetic distances. Based on geographic and geological data, a hypothesis wa s posed that it is very possible that the hilly lands in the downstream of the S onghua River and the Heilong River were the center of the origin of the Chinese forest frogs in Heilongjiang Province. And mainly through the Songhua River syst em, the Chinese forest frogs dispersed into the Songneng Plain from the Sanjiang Plain whereas the Fangzheng region became a sub-center for the western dispersi on.
文摘The performance of genetic algorithm(GA) is determined by the capability of search and optimization for satisfactory solutions. The new adaptive genetic algorithm(AGA) is built for inducing suitable search and optimization relationship. The use of six fuzzy logic controllers(6FLCs) is proposed for dynamic control genetic operating parameters of a symbolic-coded GA. This paper uses AGA based on 6FLCs to deal with the travelling salesman problem (TSP). Experimental results show that AGA based on 6FLCs is more efficient than a standard GA in solving combinatorial optimization problems similar to TSP.
文摘In the flexible job-shop scheduling problem （FJSP）, each operation has to be assigned to a machine from a set of capable machines before alocating the assigned operations on all machines. To solve the multi-objective FJSP, the Grantt graph oriented string representation （GOSR） and the basic manipulation of the genetic algorithm operator are presented. An integrated operator genetic algorithm （IOGA） and its process are described. Comparison between computational results and the latest research shows that the proposed algorithm is effective in reducing the total workload of all machines, the makespan and the critical machine workload.
文摘The history of medical genetics is briefly reviewed. It is evident that medical genetics with its inseparable part, clinical genetics, started out as a cfinical science from the very beginning. Its robust development in the developed countries is the result of a close interaction between the basic sciences and clinical genetics. In China, however, clinical genetics has not received due emphasis and medical genetics is still not recognized as one of the medical specialties. This is in marked contrast to the situation in the West. It is high time to acknowledge that medical genetics is a medical specialty and to promote clinical genetics service in qualified hospitals in our country.
基金his article was supported by the Natural Science Founda-tion of Yunnan Province (No.98C017Q).
文摘Genetic diversity and genetic differentiation of natural populations of Pinus kesiya var. langbinanensis were examined by means of electrophoresis technique. Analysis of 9 enzyme systems including 16 loci showed that all the three natural popu-lations of the pine were high in genetic diversity but low in inter-population genetic differentiation. The proportion of polymorphic loci is 0.667, with each locus holding 2.13 alleles, averagely. The average expected and observed heterozygosity was 0.288 and 0.197, respectively. The gene differentiation among populations was 0.052, but the mean genetic distance was only 0.015.
基金The research was supported by Science Foundation of Northeast Forestry University (2004)
文摘Polymorphism of nine strains （CF05, CF09, 29, 916, AU9, Chang10, Chang7, 8808 and AU. Japanese） of A. auricular cultivated in Heilongjiang Province were analyzed by RAPD （Random Amplication polymorphic DNA）. Thirteen primers were selected from forty PCR primers with 10bp long random primer. The results showed that nine strains of A. auricular have a high level of genetic diversity and the percentage of DNA polymorphic was 96.05. The genotypes of 9 strains of Auricularia auricular were identified by the fingerprints from primer 27 and primer 46 by RAPD analysis. The results are helpful for quickly identifying strains of A. auricular in its early breeding time, and also provides a powerful theoretic basis to differentiate strains （Auricularia auricular） whose morphology is very similar in breeding programs of edible fungus.
文摘Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many uses, easiness of tissue culture and small gene group that make them well suited as a model system for the application of genetic engineering in forest trees. In the last decade, much progress has been made in genetic improvement of poplar species in China. Modern biotechnology is an important tool for genetic improvement in forest trees, and its applications to genetic improvement in poplars, which covers genetic transformation, gene expression, construction of genetic linkage map, QTLs (quantitative trait loci) identification and molecular assisted selection are reviewed in this paper. At the same time, the existing problems and outlook about the application of modern biotechnology to genetic improvement in forest trees are also discussed.
文摘Microsatellite loci distributing on genome randomly act as effective genetic markers. To date, about 200 microsatellite loci were found in cervids b y transferring microsatellite PCR primers derived in bovine, ovine to cervids, a s well as a few loci derived directly from deer microsatellite library. These lo ci have been used in parentage determination, genetic diversity and population s tructure, population introgression, as genetic marker gestation length and winte ring survival et al. However, microsatellite loci presently found are untouchabl e to the demand of application. Future work should include: 1) isolating a large number of cervine microsatellite loci, 2) constructing genetic and physical map s of microsatellite loci. So that microsatelites have a strong base for advanced applications in deer.
基金Supported by the Natural Science Foundation of Jiangxi Province(2009GZC0104)the Science and Technology Research Project of Jiangxi Provincial Department of Education(GJJ10521)~~
文摘Proper fixture design is crucial to obtain the better product quality according to the design specification during the workpiece fabrication. Locator layout planning is one of the most important tasks in the fixture design process. However, the design of a fixture relies heavily on the designerts expertise and experience up to now. Therefore, a new approach to loeator layout determination for workpieces with arbitrary complex surfaces is pro- posed for the first time. Firstly, based on the fuzzy judgment method, the proper locating reference and locator - numbers are determined with consideration of surface type, surface area and position tolerance. Secondly, the lo- cator positions are optimized by genetic algorithm（GA）. Finally, a typical example shows that the approach is su- perior to the experiential method and can improve positioning accuracy effectively.
文摘Two dimensional(2 D) entropy method has to pay the price of time when applied to image segmentation. So the genetic algorithm is introduced to improve the computational efficiency of the 2 D entropy method. The proposed method uses both the gray value of a pixel and the local average gray value of an image. At the same time, the simple genetic algorithm is improved by using better reproduction and crossover operators. Thus the proposed method makes up the 2 D entropy method’s drawback of being time consuming, and yields satisfactory segmentation results. Experimental results show that the proposed method can save computational time when it provides good quality segmentation.
文摘With completion of the Populus genome sequencing project and the availability of many expressed sequence tags （ESTs） databases in forest trees, attention is now rapidly shifting towards the study of individual genetic variation in natural populations. The most abundant form of genetic variation in many eukaryotic species is represented by single nucleotide polymorphisms （SNPs）, which can account for heritable inter-individual differences in complex phenotypes. Unlike humans, the linkage disequilibrium （LD） rapidly decays within candidate genes in forest trees. Thus, SNPs-based candidate gene association studies are considered to be a most effective approach to dissect the complex quantitative traits in forest trees. The present study demonstrates that LD mapping can be used to identify alleles associated with quantitative traits and suggests that this new approach could be particularly useful for performing breeding programs in forest trees. In this review, we will describe the fundamentals, patterns of SNPs distribution and frequency, summarize recent advances in SNPs discovery and LD and comment on the application of LD in the dissection of complex quantitative traits in forest tress. We also put forward the outlook for future SNPs-based association analysis of quantitative traits in forest trees.
文摘BACKGROUND： The tumor necrosis factor recepter associated factor （TRAF） 6 is an important intracellular adapter protein that plays a pivotal role in activating multiple inflammatory and immune related processes induced by cytokines. TRAF6 represents a strong candidate susceptibility factor for sepsis. We investigated whether polymorphisms at the TRAF6 gene are associated with the susceptibility to and severity of sepsis.METHODS： A hospital-based case-control study was conducted with 255 patients with sepsis and 260 controls who were recruited from Zhengzhou, China. Haplotype tagging single nucleotide polymorphisms （htSNPs） were selected from the HapMap database and genotyped using the SNPstream genotyping platform. The associations with the susceptibility and disease severity of sepsis were estimated by logistic regression, and adjusted for age, sex, smoking, drinking, chronic diseases status, APACHEII score and critical illness status.RESULTS： A total of 13 TRAF6 SNPs were tagged by 7 htSNPs. Five htSNPs （rs5030490, rs5030411, rs5030416, rs5030445 and rs3740961） were genotyped in the case control study. Genotype frequencies of the htSNPs were conformed to the Hardy-Weinberg equilibrium in both patients and controls. No significant association was found between the 5 htSNPs and the susceptibility to and severity of sepsis. Compared with the main haplotype -11120A/-10688T/-9423A/805G/12967G, no certain haplotype was associated with the signi？ cantly susceptibility to or severity of sepsis.CONCLUSION： TRAF6 gene polymorphisms might not play a major role in mediating the susceptibility to and severity of sepsis in the Chinese population. A larger population-based case-control study is warranted.
