A simple method for the determination of putrescine,spermidine and spermine in hair sample was developed by gas chromatography-mass spectrometry(GC-MS).After alkaline digestion,hair samples were extracted by solid pha...A simple method for the determination of putrescine,spermidine and spermine in hair sample was developed by gas chromatography-mass spectrometry(GC-MS).After alkaline digestion,hair samples were extracted by solid phase extraction(SPE) with diatomaceous earth column,and then derivatized with heptafluorobutyryl chloride(HFB-Cl) in n-hexane.The analytes were determined by a mass spectrometer detector in selected ion monitoring(SIM) mode.A good reproducibility and accuracy were attained.The method was applied to the analysis of the hairs of 14 healthy men and 22 healthy women.Results showed that the most abundant polyamine was spermidine,followed by putrescine and spermine,and the levels of three hair polyamines in men were all found to be higher than in women,respectively.展开更多
The endogenous polyamine levels were tested at every developmental stage in different ecotypes of the Chinese cabbage. The results showed that polyamincs (PAs) were decreased in the process of flower bud initiation an...The endogenous polyamine levels were tested at every developmental stage in different ecotypes of the Chinese cabbage. The results showed that polyamincs (PAs) were decreased in the process of flower bud initiation and fornfation, but the floral stem formation and elongation were accompied by PAs increase. The level of Spm was related to the bolting date of Chinese cabbage. During the inducing of flower bud initiation and elongation of floral stem, and the changes of Spm were reversed between early bolting date and late bolting cultivars, but they have the same requirment for Spd for the starting of flower bud initiation and the starting of bolting. The level of Spd changed little during entire developmental stage for early bolting cultivars, but a lot for late bolting cultivars. The time that the highest level of Put appeared is related to the bolting date of Chinese cabbage.展开更多
文摘A simple method for the determination of putrescine,spermidine and spermine in hair sample was developed by gas chromatography-mass spectrometry(GC-MS).After alkaline digestion,hair samples were extracted by solid phase extraction(SPE) with diatomaceous earth column,and then derivatized with heptafluorobutyryl chloride(HFB-Cl) in n-hexane.The analytes were determined by a mass spectrometer detector in selected ion monitoring(SIM) mode.A good reproducibility and accuracy were attained.The method was applied to the analysis of the hairs of 14 healthy men and 22 healthy women.Results showed that the most abundant polyamine was spermidine,followed by putrescine and spermine,and the levels of three hair polyamines in men were all found to be higher than in women,respectively.
基金The results is supported by Chinese natural science foundation
文摘The endogenous polyamine levels were tested at every developmental stage in different ecotypes of the Chinese cabbage. The results showed that polyamincs (PAs) were decreased in the process of flower bud initiation and fornfation, but the floral stem formation and elongation were accompied by PAs increase. The level of Spm was related to the bolting date of Chinese cabbage. During the inducing of flower bud initiation and elongation of floral stem, and the changes of Spm were reversed between early bolting date and late bolting cultivars, but they have the same requirment for Spd for the starting of flower bud initiation and the starting of bolting. The level of Spd changed little during entire developmental stage for early bolting cultivars, but a lot for late bolting cultivars. The time that the highest level of Put appeared is related to the bolting date of Chinese cabbage.
