A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, ba...A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, basal medium formula and carbohydrate levels were examined to determine their various effects on suspension culture cell growth and development. The best selection of plant growth regulator, hasal medium and carbohydrate level is 2 mg / L 2,4-D: 0.5 mg / L KT,MS and 3% sucrose respectively.展开更多
为探究不同植物生长调节剂处理对辣椒挥发性香气物质的影响,采用顶空固相微萃取-气相色谱-质谱(headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry,HS-SPME-GC-MS)联用技术,通过化学计量学分...为探究不同植物生长调节剂处理对辣椒挥发性香气物质的影响,采用顶空固相微萃取-气相色谱-质谱(headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry,HS-SPME-GC-MS)联用技术,通过化学计量学分析对复硝酚钠、赤霉素、芸苔素内酯处理的辣椒以及对照辣椒挥发性香气物质进行综合评价。结果表明,在4种处理中共检测出43种挥发性香气物质,其中对照组38种、复硝酚钠组40种、芸苔素内酯和赤霉素组43种。芸苔素内酯和赤霉素处理提高了辣椒挥发性香气物质的总含量,复硝酚钠处理的总含量无差异。以PLS-DA模型的变量重要性投影VIP值>1.0为条件,共筛选出12种差异挥发物,对挥发性香气成分进行主成分分析并构建主成分综合评价函数,香气品质综合得分最高为芸苔素内酯组,其次为赤霉素组。研究结果为植物生长调节剂的合理、高效利用提供了理论依据,有助于促进辣椒优质产品的生产。展开更多
文摘A cell suspension culture of Panax ginseng which may be continuously subcultured has been established. Embryogenic callus derived from clutured young leaves was used to initiate the culture.Plant growth regulators, basal medium formula and carbohydrate levels were examined to determine their various effects on suspension culture cell growth and development. The best selection of plant growth regulator, hasal medium and carbohydrate level is 2 mg / L 2,4-D: 0.5 mg / L KT,MS and 3% sucrose respectively.
文摘为探究不同植物生长调节剂处理对辣椒挥发性香气物质的影响,采用顶空固相微萃取-气相色谱-质谱(headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry,HS-SPME-GC-MS)联用技术,通过化学计量学分析对复硝酚钠、赤霉素、芸苔素内酯处理的辣椒以及对照辣椒挥发性香气物质进行综合评价。结果表明,在4种处理中共检测出43种挥发性香气物质,其中对照组38种、复硝酚钠组40种、芸苔素内酯和赤霉素组43种。芸苔素内酯和赤霉素处理提高了辣椒挥发性香气物质的总含量,复硝酚钠处理的总含量无差异。以PLS-DA模型的变量重要性投影VIP值>1.0为条件,共筛选出12种差异挥发物,对挥发性香气成分进行主成分分析并构建主成分综合评价函数,香气品质综合得分最高为芸苔素内酯组,其次为赤霉素组。研究结果为植物生长调节剂的合理、高效利用提供了理论依据,有助于促进辣椒优质产品的生产。
