Tree peony is well known and sought after for its large, colorful flowers. Its propagation is via vegetative methods. Mech- nisms of the adventitious rooting and the regulation of rooting processes are the principles ...Tree peony is well known and sought after for its large, colorful flowers. Its propagation is via vegetative methods. Mech- nisms of the adventitious rooting and the regulation of rooting processes are the principles and techniques of plant propagation and improvement. Microstructures and fluctuations of phytohormones in the adventitious rooting were studied with the etiolated soft- wood shoots of Paeonia suffkuticosa ＇Yinfen Jinlin＇. There are no pre-primordia in the shoots of the cultivar. Adventitious roots are produced in five stages： shoot selection, primordium initiation, primordium growth, conducting tissue differentiation and root protru- sion. Primordia initiated in the cortex. The contents of the endogenous hormones, IAA, ABA and GA, were 5.842, 0.873 and 1.043 nmol·g^-1 FW on the bases of shoots, respectively. CTKs which included isopentenyl adenine （iPA）, zeatin riboside （ZR） and dihy- drozeatin riboside （DHZR） were 0.949, 0.695 and 2.034 nmol·g^-1 FW, respectively. DHZR is active among CTKs. The ratio of IAA to GA, CTK and ABA clearly increased at the stage of primordium initiation, while they showed low levels at the stages of primor- dium growth. The ratios were restored at the shoot levels at the stage of root protrusion. IBA provoked primordia initiation in the cortex, the vascular cambium, the pith and even in the callus induced on the base of shoots. ]AA levels in the treated shoots increased gradually to its highest level （three times of control） at the stage of conducting tissue differentiation. The ratios of IAA to GA, CTK and ABA clearly decreased at the stage of primordium initiation. The ratio of IAA to ABA is regulated at 10：1.展开更多
The objectives of this study were to investigate the effects of successive continuous generation （SCG） cuttings of Buxus sinica var. parvifolia on the rejuvenation of ortets at the hormone level, detect levels of in...The objectives of this study were to investigate the effects of successive continuous generation （SCG） cuttings of Buxus sinica var. parvifolia on the rejuvenation of ortets at the hormone level, detect levels of indoleacetic acid （IAA）, abscisic acid （ABA）, isopentenyladenosine （iPA）, zeatin riboside （ZR） and gibberellin4 （GA4） during the rooting process of different generations by means of enzyme-linked immunosorbent assay （ELISA） technology and statistically analyze the rooting characteristics of cuttings. The results show that, the root systems of subsequent generations （generation 1998, 2000） developed satisfactorily, only a few initial generation （generation 1990, 1994） cuttings rooted and the root growth was inhibited. Around the period of callus formation and root projection through the epidermis, there was a marked, regular variation in the levels of IAA, ABA and GA4 between the initial generations and subsequent generations.展开更多
Plasma membrane vesicles were prepared from the developing cotyledons of common beau (Phaseolus vulgaris L cv Diyundou) by aqueous two-phase partitioning and characterized as to their purity by assaying marker enzymes...Plasma membrane vesicles were prepared from the developing cotyledons of common beau (Phaseolus vulgaris L cv Diyundou) by aqueous two-phase partitioning and characterized as to their purity by assaying marker enzymes for other membranes.The putative plasma membrane fraction was minimally contaminated by membranes Other than plasma membrane and hence was of high purity. It exhibited a Ca2+dependent ATPase activity, which was inhibited by 1μ mol/L EB and promoted by calcium ionophore A23187. Such an activity was responsible for the observed ATPdependent 45Ca2+ uptake into inside-out plasma membrane vesicles. This process was stimulated by 0.6μmol/L CaM and 20μmol/L IAA but inhibited by 2μmol/L ABA and abolished by A23187. Possible role of cytoplasmic Ca2+ in mediating phytohormones activity is discussed.展开更多
Tissue culture of Antirrhium majus was experimented by using the tip of shoots as the explants, and comparing the effects of NAA, IAA and 2, 4-D on the formation of adventitious buds. The results indicated that the ef...Tissue culture of Antirrhium majus was experimented by using the tip of shoots as the explants, and comparing the effects of NAA, IAA and 2, 4-D on the formation of adventitious buds. The results indicated that the effect of NAA was the best for the formation of adventitious buds, 5.0, 7.0, 9.0, 11.0, 13.0 mg/L BA combinatcd with 0.1, 0.5, 1.0, 1.5, 2.0 mg/L NAA respectively. The results show that combinations of the concentrations of BA 7.0-9.0 mg/L and NAA 0.1-0.5 mg/L were advantageous to the regeneration of buds and NAA was very important for callus growth.展开更多
文摘Tree peony is well known and sought after for its large, colorful flowers. Its propagation is via vegetative methods. Mech- nisms of the adventitious rooting and the regulation of rooting processes are the principles and techniques of plant propagation and improvement. Microstructures and fluctuations of phytohormones in the adventitious rooting were studied with the etiolated soft- wood shoots of Paeonia suffkuticosa ＇Yinfen Jinlin＇. There are no pre-primordia in the shoots of the cultivar. Adventitious roots are produced in five stages： shoot selection, primordium initiation, primordium growth, conducting tissue differentiation and root protru- sion. Primordia initiated in the cortex. The contents of the endogenous hormones, IAA, ABA and GA, were 5.842, 0.873 and 1.043 nmol·g^-1 FW on the bases of shoots, respectively. CTKs which included isopentenyl adenine （iPA）, zeatin riboside （ZR） and dihy- drozeatin riboside （DHZR） were 0.949, 0.695 and 2.034 nmol·g^-1 FW, respectively. DHZR is active among CTKs. The ratio of IAA to GA, CTK and ABA clearly increased at the stage of primordium initiation, while they showed low levels at the stages of primor- dium growth. The ratios were restored at the shoot levels at the stage of root protrusion. IBA provoked primordia initiation in the cortex, the vascular cambium, the pith and even in the callus induced on the base of shoots. ]AA levels in the treated shoots increased gradually to its highest level （three times of control） at the stage of conducting tissue differentiation. The ratios of IAA to GA, CTK and ABA clearly decreased at the stage of primordium initiation. The ratio of IAA to ABA is regulated at 10：1.
基金the National Natural Science Foundation of China （Grant No. 30070633）.
文摘The objectives of this study were to investigate the effects of successive continuous generation （SCG） cuttings of Buxus sinica var. parvifolia on the rejuvenation of ortets at the hormone level, detect levels of indoleacetic acid （IAA）, abscisic acid （ABA）, isopentenyladenosine （iPA）, zeatin riboside （ZR） and gibberellin4 （GA4） during the rooting process of different generations by means of enzyme-linked immunosorbent assay （ELISA） technology and statistically analyze the rooting characteristics of cuttings. The results show that, the root systems of subsequent generations （generation 1998, 2000） developed satisfactorily, only a few initial generation （generation 1990, 1994） cuttings rooted and the root growth was inhibited. Around the period of callus formation and root projection through the epidermis, there was a marked, regular variation in the levels of IAA, ABA and GA4 between the initial generations and subsequent generations.
文摘Plasma membrane vesicles were prepared from the developing cotyledons of common beau (Phaseolus vulgaris L cv Diyundou) by aqueous two-phase partitioning and characterized as to their purity by assaying marker enzymes for other membranes.The putative plasma membrane fraction was minimally contaminated by membranes Other than plasma membrane and hence was of high purity. It exhibited a Ca2+dependent ATPase activity, which was inhibited by 1μ mol/L EB and promoted by calcium ionophore A23187. Such an activity was responsible for the observed ATPdependent 45Ca2+ uptake into inside-out plasma membrane vesicles. This process was stimulated by 0.6μmol/L CaM and 20μmol/L IAA but inhibited by 2μmol/L ABA and abolished by A23187. Possible role of cytoplasmic Ca2+ in mediating phytohormones activity is discussed.
文摘Tissue culture of Antirrhium majus was experimented by using the tip of shoots as the explants, and comparing the effects of NAA, IAA and 2, 4-D on the formation of adventitious buds. The results indicated that the effect of NAA was the best for the formation of adventitious buds, 5.0, 7.0, 9.0, 11.0, 13.0 mg/L BA combinatcd with 0.1, 0.5, 1.0, 1.5, 2.0 mg/L NAA respectively. The results show that combinations of the concentrations of BA 7.0-9.0 mg/L and NAA 0.1-0.5 mg/L were advantageous to the regeneration of buds and NAA was very important for callus growth.
