An orthogonal design was used to optimize SSR-PCR amplification system using Panax ginseng genomic DNA as template. Four levels of five factors （DNA template, Taq DNA polymerase, Mg^2＋, primer, and dNTP） and anneal...An orthogonal design was used to optimize SSR-PCR amplification system using Panax ginseng genomic DNA as template. Four levels of five factors （DNA template, Taq DNA polymerase, Mg^2＋, primer, and dNTP） and annealing temperature have been tested separately in this system. The results demonstrated the reaction efficiency was affected by these factors. Based on the results, a stable, productive and reproducible PCR system and cycling program for amplifying a ginseng SSR locus were obtained： 20 μL system containing 1.0 U Taq DNA polymerase, 2.0 mmol·L^-1 Mg^2＋, 0.2 mmol·L^-1 dNTPs, 0.3 μmol·L^-1 SSR primer, 60 ng· μla^-1 DNA template, performed with a program of 94℃ for 5 min, 94℃ for 30 s, annealing at 56.3℃ for 30 s, 72℃ for 1 min, 37 cycles, finishing at 72℃ for 7 min, and storing at 4℃.展开更多
A biconical liner was optimized to improve its penetration ability. Its formation and pene- tration mechanism was studied through numerical simulation and experiments. And the influence of different liner geometry siz...A biconical liner was optimized to improve its penetration ability. Its formation and pene- tration mechanism was studied through numerical simulation and experiments. And the influence of different liner geometry sizes on the jet performance was analyzed using the orthogonal method. The liner formed the high-speed jet with an explosively formed projectile. The small angle 2a remarkably influenced the jet speed which was inversely proportional to 2a. And the liner thickness t＇ and large angle 2β had highly significant effect on the projectile speed. The liner was optimized at t＇ = 0. 14 cm, 2α = 50°, 2β = 135°, N = 0. 4 or 0. 5, when its jet speed respectively is at 6 613 m/s and 6 839 m/s and projectile speed is at 2 247 m/s and 2 095 m/s, steel target penetration is at 8. 24 cm and 8. 31 cm, and aperture is in 2.12 cm and 2.08 cm. The results show that target is penetrated by the high-speed jet and high-speed projectile resulting into double damages. The penetration ability is improved greatly.展开更多
Elastic anisotropy of shales is critical to accurate constraints for rock physical models,quantitative interpretation and hydraulic fracturing.However,the causes of elastic anisotropy of shales are very complicated,an...Elastic anisotropy of shales is critical to accurate constraints for rock physical models,quantitative interpretation and hydraulic fracturing.However,the causes of elastic anisotropy of shales are very complicated,and the understanding of how multiple influence factors affect the elastic anisotropy of shales is still not clear.Hence,the orthogonal experiment,as an effective multiple factors experimental method,is adopted in this study to analyze the effect of multiple factors for shale elastic anisotropy.Three factors,clay content,organic matter(OM)content and compaction stress are selected as independent variables,the orthogonal test table L_(16)(4^(3))with four levels for each factor is adopted.According to the designed orthogonal table,sixteen artificial shales are constructed based on the cold-pressing method,and all the dry artificial shales are measured by the ultrasonic measurements.The influence of each factor on the elastic anisotropy and the sensitivity orders of three factors are obtained using the range analysis.The orders of sensitivity for selected factors follow the sequence clay content>compaction stress>OM content for velocity anisotropy parameters.The compaction mechanism of artificial shales is also discussed by the compaction factor,which are positively correlated with the velocity anisotropy parameters.The clay platelets orientation distribution function(ODF)of samples is evaluated by a theoretical model,the ODF coefficients are significantly affected by the clay content and compaction stress,and W200 are much more sensitive to these factors than W400.The results can provide a critical rock physics basis for quantitative interpretation and reservoir prediction of the low-maturity or maturity shale reservoir.展开更多
Three-factor orthogonal design（OD） of Er3＋/Gd3＋/T（calcination temperature） is used to optimize the luminescent intensity of Na Y（Gd）（MoO4）2：Er3＋phosphor.Firstly,the uniform design（UD） is introduced to ex...Three-factor orthogonal design（OD） of Er3＋/Gd3＋/T（calcination temperature） is used to optimize the luminescent intensity of Na Y（Gd）（MoO4）2：Er3＋phosphor.Firstly,the uniform design（UD） is introduced to explore the doping concentration range of Er3＋/Gd3＋.Then OD and range analysis are performed based on the results of UD to obtain the primary and secondary sequence and the best combination of Er3＋,Gd3＋,and T within the experimental range.The optimum sample is prepared by the high temperature solid state method.Photoluminescence excitation and emission spectra of the optimum sample are detected.The intense green emissions（530 nm and 550 nm） are observed which originate from Er3＋2H11/2→4I15/2and4S3/2→4I15/2,respectively.Thermal effect is investigated in the optimum NaY（Gd3＋）（MoO4）2：Er3＋phosphors,and the green emission intensity decreases as temperature increases.展开更多
Soil samples were taken from depth of 0-12 cm in virgin broad-leaved Korean pine mixed forest in Changbai Moun-tain in July 2000. The effects of temperature, soil water content, pH, NH4+ and NO3- on N2O emission and C...Soil samples were taken from depth of 0-12 cm in virgin broad-leaved Korean pine mixed forest in Changbai Moun-tain in July 2000. The effects of temperature, soil water content, pH, NH4+ and NO3- on N2O emission and CH4 uptake of a for-est soil were studied in laboratory by the method of orthogonal design. It was observed under laboratory conditions in this study that there were significant correlations between N2O emission rate, CH4 oxidation rate, soil pH and temperature. Nevertheless, N2O emission rate also showed a significant positive correlation with CH4 oxidation rate. The results suggested that pH and temperature were important factors controlling N2O emission and CH4 oxidation under this experiment conditions.展开更多
We studied how bioflocculants,produced by white-rot fungi,affect flocculation in slime water.Based on a test in an orthogonal design,flocculation conditions were optimized.The results show that flocculation activity i...We studied how bioflocculants,produced by white-rot fungi,affect flocculation in slime water.Based on a test in an orthogonal design,flocculation conditions were optimized.The results show that flocculation activity is at its highest when the following conditions are met:slime water concentration 27.42 g/L;coagulant aid(CaCl_2) mass concentration 5.0 g/L;two-segment stirrings:the first at a stirring speed of 60 r/min for 180 s and the second 180 r/min for 60 s;a pH of 11 and a flocculant concentration of 15 mL/L.The flocculation activity can be up to 98.71%of bioflocculants at the time.Further experiments indicate that most of the flocculation active material is found outside the mycelium cells.This is the extracellular secretion produced by mycelium cells during the fermentation process.This flocculant has strong thermal stability.Many kinds of cations have a flocculation function to assist bioflocculants.This aid-flocculation effect of the divalent cation Ca^(2+) is obvious in the bioflocculant produced by the white-rot fungus.Therefore,this is of great value when applied to control engineering in the battle against water pollution.展开更多
Urea-formaldehyde （UF） adhesive is the main source of formaldehyde emission from UF-bonded boards. The components in waste tea leaves can react with formaldehyde to serve as a raw material in the production of low f...Urea-formaldehyde （UF） adhesive is the main source of formaldehyde emission from UF-bonded boards. The components in waste tea leaves can react with formaldehyde to serve as a raw material in the production of low formaldehyde emission boards. In our study, waste tea leaves and UF adhesive were employed in the preparation of waste tea leaves particleboard （WTLB）. An orthogonal experimental method was applied to investigate the effects of process parameters on formaldehyde emission and mechanical properties of WTLB. The results indicated that： 1） waste tea leaves had the ability to abate formaldehyde emission from boards; and 2） density of the WTLB was a significant factor affecting its modulus of rupture （MOR）, modulus of elasticity （MOE） and internal bonding （IB）.展开更多
By orthogonal design theory, technological parameters of chrysanthemum-like ZnO particles prepared in a hydrothermal process are optimized. This paper reports a set of technological parameters for growing chrysanthemu...By orthogonal design theory, technological parameters of chrysanthemum-like ZnO particles prepared in a hydrothermal process are optimized. This paper reports a set of technological parameters for growing chrysanthemumlike ZnO particles on a large scale. It investigates the morphologies and crystalline structures of the as-synthesized three-dimensional ZnO particles with a scanning electron microscope, x-ray diffractometer and transmission electron microscope, and the possible growth mechanism on the three-dimensional ZnO particles. The experimental results indicate that the values of ε′ε″ and tan δe gradually increase in the X band with the improvement of the developmental level of chrysanthemum-like ZnO particles, implying that the electromagnetic wave absorbing property depends on the morphologies of three-dimensional ZnO particles.展开更多
An infinite family of is obtained, that is,(ν)≠￠ forυ ∈ N=N1UN2 U N3 where Using(υ),we give 2-(2υ;k1,k2;k1 + k2 -υ} supplementary difference sets withυ=(k1-υ)2 + (k2 - υ)2. Finally. we prove that if there ...An infinite family of is obtained, that is,(ν)≠￠ forυ ∈ N=N1UN2 U N3 where Using(υ),we give 2-(2υ;k1,k2;k1 + k2 -υ} supplementary difference sets withυ=(k1-υ)2 + (k2 - υ)2. Finally. we prove that if there exists an orthogonal design OD(4t;t,t,t,t) and(υ) ≠￠ then a Hadamard rnatrix of order 4tυ can be constructed.展开更多
Boiling heat transfer and the controllability of the thermal load of the cylinder head were studied.The thermodynamic phase change characteristics of the cylinder head coolant were considered and the mass,momentumand ...Boiling heat transfer and the controllability of the thermal load of the cylinder head were studied.The thermodynamic phase change characteristics of the cylinder head coolant were considered and the mass,momentumand energy transfers between two phases were calculated with the interface transfer submodels by using the computational fluid dynamics software CFX. Results showed that compared with the single-phase flow without considering the boiling heat transfer,the sub-cooled boiling heat transfer of the cylinder head was greatly increased. According to the results of the numerical simulation,an optimized structure of the water jacket was proposed. Finally,temperature and velocity of coolant,diameter of flow passage and mean bubble diameter that influences sub-cooled boiling were studied using the orthogonal experiment method.展开更多
Aiming at the independent development of tracked vehicles,it is urgent to improve its mobility,passability and ride comfort,a new type of flexible road wheel with a“wheel-hinge-hub”combined structure is proposed in ...Aiming at the independent development of tracked vehicles,it is urgent to improve its mobility,passability and ride comfort,a new type of flexible road wheel with a“wheel-hinge-hub”combined structure is proposed in this study.The vibration model characteristics of the flexible road wheel were studied by the combination of numerical simulation and experiments.The superelasticity of rubber is obtained through uniaxial tensile experiment of the material and a detail three-dimensional nolinear finite element model of the flexible road wheel is established through finite element software ABAQUS.The free vibration equation of the flexible road wheel is solved by Lanczos vector direct superposition method,and its predicted modes and natural frequencies are compared with experimental results,which verifies the accuracy and reliability of the established finite element model.On this basis,the effects of various key structural or material factors on the natural frequencies of the flexible road wheel are studied using orthogonal experimental design method.Besides,the vibration modal characteristics of the flexible road wheel are also compared with those of the rigid road wheel.The research results provide a theoretical basis for the vibration and noise reduction of flexible road wheel.展开更多
A new process for preparing poly(phenylene sulfide amide, PPSA), which is by reaction of sulfur instead of sodium sulfide as S source with dichlorobenzamide (DCBA) and alkali in polar orga...A new process for preparing poly(phenylene sulfide amide, PPSA), which is by reaction of sulfur instead of sodium sulfide as S source with dichlorobenzamide (DCBA) and alkali in polar organic solvent at the atmospheric pressure (called sulfur solution route), is reported in the present paper. The influences of polymerization time, molar ratio of precursors, catalyst and solvent upon the polymer were investigated. To seek the best parameters of polymerization, orthogonal design was employed in the experiments. The results indicate that the molar ratio of precursors is the most significant effect on both of viscosity and yield of the polymer. The suitable parameters for preparing the related polymer are presented. The polymer was characterized by IRspectrum, 1HNMRspectrum and Raman spectrum, etc.展开更多
基金This research was supported by Department of Wildlife Conservation, State Forestry Administration, P. R. China.
文摘An orthogonal design was used to optimize SSR-PCR amplification system using Panax ginseng genomic DNA as template. Four levels of five factors （DNA template, Taq DNA polymerase, Mg^2＋, primer, and dNTP） and annealing temperature have been tested separately in this system. The results demonstrated the reaction efficiency was affected by these factors. Based on the results, a stable, productive and reproducible PCR system and cycling program for amplifying a ginseng SSR locus were obtained： 20 μL system containing 1.0 U Taq DNA polymerase, 2.0 mmol·L^-1 Mg^2＋, 0.2 mmol·L^-1 dNTPs, 0.3 μmol·L^-1 SSR primer, 60 ng· μla^-1 DNA template, performed with a program of 94℃ for 5 min, 94℃ for 30 s, annealing at 56.3℃ for 30 s, 72℃ for 1 min, 37 cycles, finishing at 72℃ for 7 min, and storing at 4℃.
基金Supported by the National Natural Science Foundation of China(51174183)the Anhui Natural Science Foundation of China(1508085ME85)
文摘A biconical liner was optimized to improve its penetration ability. Its formation and pene- tration mechanism was studied through numerical simulation and experiments. And the influence of different liner geometry sizes on the jet performance was analyzed using the orthogonal method. The liner formed the high-speed jet with an explosively formed projectile. The small angle 2a remarkably influenced the jet speed which was inversely proportional to 2a. And the liner thickness t＇ and large angle 2β had highly significant effect on the projectile speed. The liner was optimized at t＇ = 0. 14 cm, 2α = 50°, 2β = 135°, N = 0. 4 or 0. 5, when its jet speed respectively is at 6 613 m/s and 6 839 m/s and projectile speed is at 2 247 m/s and 2 095 m/s, steel target penetration is at 8. 24 cm and 8. 31 cm, and aperture is in 2.12 cm and 2.08 cm. The results show that target is penetrated by the high-speed jet and high-speed projectile resulting into double damages. The penetration ability is improved greatly.
基金supported by the National Natural Science Fund Projects(42104107)the Fundamental Research Funds for the Central Universities(2022XJDC06).
文摘Elastic anisotropy of shales is critical to accurate constraints for rock physical models,quantitative interpretation and hydraulic fracturing.However,the causes of elastic anisotropy of shales are very complicated,and the understanding of how multiple influence factors affect the elastic anisotropy of shales is still not clear.Hence,the orthogonal experiment,as an effective multiple factors experimental method,is adopted in this study to analyze the effect of multiple factors for shale elastic anisotropy.Three factors,clay content,organic matter(OM)content and compaction stress are selected as independent variables,the orthogonal test table L_(16)(4^(3))with four levels for each factor is adopted.According to the designed orthogonal table,sixteen artificial shales are constructed based on the cold-pressing method,and all the dry artificial shales are measured by the ultrasonic measurements.The influence of each factor on the elastic anisotropy and the sensitivity orders of three factors are obtained using the range analysis.The orders of sensitivity for selected factors follow the sequence clay content>compaction stress>OM content for velocity anisotropy parameters.The compaction mechanism of artificial shales is also discussed by the compaction factor,which are positively correlated with the velocity anisotropy parameters.The clay platelets orientation distribution function(ODF)of samples is evaluated by a theoretical model,the ODF coefficients are significantly affected by the clay content and compaction stress,and W200 are much more sensitive to these factors than W400.The results can provide a critical rock physics basis for quantitative interpretation and reservoir prediction of the low-maturity or maturity shale reservoir.
基金Project supported by Education Reform Fund of Dalian Maritime University,China(Grant No.2015Y37)the Natural Science Foundation of Liaoning Province,China(Grant Nos.2015020190 and 2014025010)+1 种基金the Open Fund of the State Key Laboratory on Integrated Optoelectronics,China(Grant No.IOSKL2015KF27)the Fundamental Research Funds for the Central Universities,China(Grant No.3132016121)
文摘Three-factor orthogonal design（OD） of Er3＋/Gd3＋/T（calcination temperature） is used to optimize the luminescent intensity of Na Y（Gd）（MoO4）2：Er3＋phosphor.Firstly,the uniform design（UD） is introduced to explore the doping concentration range of Er3＋/Gd3＋.Then OD and range analysis are performed based on the results of UD to obtain the primary and secondary sequence and the best combination of Er3＋,Gd3＋,and T within the experimental range.The optimum sample is prepared by the high temperature solid state method.Photoluminescence excitation and emission spectra of the optimum sample are detected.The intense green emissions（530 nm and 550 nm） are observed which originate from Er3＋2H11/2→4I15/2and4S3/2→4I15/2,respectively.Thermal effect is investigated in the optimum NaY（Gd3＋）（MoO4）2：Er3＋phosphors,and the green emission intensity decreases as temperature increases.
基金This paper was supported by Chinese Academy of Sciences.
文摘Soil samples were taken from depth of 0-12 cm in virgin broad-leaved Korean pine mixed forest in Changbai Moun-tain in July 2000. The effects of temperature, soil water content, pH, NH4+ and NO3- on N2O emission and CH4 uptake of a for-est soil were studied in laboratory by the method of orthogonal design. It was observed under laboratory conditions in this study that there were significant correlations between N2O emission rate, CH4 oxidation rate, soil pH and temperature. Nevertheless, N2O emission rate also showed a significant positive correlation with CH4 oxidation rate. The results suggested that pH and temperature were important factors controlling N2O emission and CH4 oxidation under this experiment conditions.
基金the Shenhuo Mining Group Co.Ltd.,China for its financial support.At the same time,we also thank the National Natural Science Foundation of China(No.40373044)the Natural Science Foundation of Jiangsu Province (No.05KJD610209) for their supportthe Jiangsu Key Laboratory of Resources and Environmental Information Engineering for its technical support.
文摘We studied how bioflocculants,produced by white-rot fungi,affect flocculation in slime water.Based on a test in an orthogonal design,flocculation conditions were optimized.The results show that flocculation activity is at its highest when the following conditions are met:slime water concentration 27.42 g/L;coagulant aid(CaCl_2) mass concentration 5.0 g/L;two-segment stirrings:the first at a stirring speed of 60 r/min for 180 s and the second 180 r/min for 60 s;a pH of 11 and a flocculant concentration of 15 mL/L.The flocculation activity can be up to 98.71%of bioflocculants at the time.Further experiments indicate that most of the flocculation active material is found outside the mycelium cells.This is the extracellular secretion produced by mycelium cells during the fermentation process.This flocculant has strong thermal stability.Many kinds of cations have a flocculation function to assist bioflocculants.This aid-flocculation effect of the divalent cation Ca^(2+) is obvious in the bioflocculant produced by the white-rot fungus.Therefore,this is of great value when applied to control engineering in the battle against water pollution.
文摘Urea-formaldehyde （UF） adhesive is the main source of formaldehyde emission from UF-bonded boards. The components in waste tea leaves can react with formaldehyde to serve as a raw material in the production of low formaldehyde emission boards. In our study, waste tea leaves and UF adhesive were employed in the preparation of waste tea leaves particleboard （WTLB）. An orthogonal experimental method was applied to investigate the effects of process parameters on formaldehyde emission and mechanical properties of WTLB. The results indicated that： 1） waste tea leaves had the ability to abate formaldehyde emission from boards; and 2） density of the WTLB was a significant factor affecting its modulus of rupture （MOR）, modulus of elasticity （MOE） and internal bonding （IB）.
基金supported by the National High Technology Research and Development Program of China (Grant No 2005A000200)the Xi’an Applied Materials Innovation Fund (Grant No XA-AM-200712)the Innovative Experiment Program for Chinese students (Grant No State 200721)
文摘By orthogonal design theory, technological parameters of chrysanthemum-like ZnO particles prepared in a hydrothermal process are optimized. This paper reports a set of technological parameters for growing chrysanthemumlike ZnO particles on a large scale. It investigates the morphologies and crystalline structures of the as-synthesized three-dimensional ZnO particles with a scanning electron microscope, x-ray diffractometer and transmission electron microscope, and the possible growth mechanism on the three-dimensional ZnO particles. The experimental results indicate that the values of ε′ε″ and tan δe gradually increase in the X band with the improvement of the developmental level of chrysanthemum-like ZnO particles, implying that the electromagnetic wave absorbing property depends on the morphologies of three-dimensional ZnO particles.
文摘An infinite family of is obtained, that is,(ν)≠￠ forυ ∈ N=N1UN2 U N3 where Using(υ),we give 2-(2υ;k1,k2;k1 + k2 -υ} supplementary difference sets withυ=(k1-υ)2 + (k2 - υ)2. Finally. we prove that if there exists an orthogonal design OD(4t;t,t,t,t) and(υ) ≠￠ then a Hadamard rnatrix of order 4tυ can be constructed.
基金Supported by the National Key Basic Research Program of China(1030021210710)
文摘Boiling heat transfer and the controllability of the thermal load of the cylinder head were studied.The thermodynamic phase change characteristics of the cylinder head coolant were considered and the mass,momentumand energy transfers between two phases were calculated with the interface transfer submodels by using the computational fluid dynamics software CFX. Results showed that compared with the single-phase flow without considering the boiling heat transfer,the sub-cooled boiling heat transfer of the cylinder head was greatly increased. According to the results of the numerical simulation,an optimized structure of the water jacket was proposed. Finally,temperature and velocity of coolant,diameter of flow passage and mean bubble diameter that influences sub-cooled boiling were studied using the orthogonal experiment method.
基金the National Natural Science Foundation of China[grant numbers 11672127,51605215]the Advance Research Special Technology Project of Army Equipment[grant number AGA19001]+2 种基金the Army Research and Technology Project[grant number AQA19001],the Innovation Fund Project of China Aerospace 1st Academy[grant number CHC20001]the Special funded project of China Postdoctoral Science Foundation[grant number 2019T120450]the Fundamental Research Funds for the Central Universities[grant number NP2020407].
文摘Aiming at the independent development of tracked vehicles,it is urgent to improve its mobility,passability and ride comfort,a new type of flexible road wheel with a“wheel-hinge-hub”combined structure is proposed in this study.The vibration model characteristics of the flexible road wheel were studied by the combination of numerical simulation and experiments.The superelasticity of rubber is obtained through uniaxial tensile experiment of the material and a detail three-dimensional nolinear finite element model of the flexible road wheel is established through finite element software ABAQUS.The free vibration equation of the flexible road wheel is solved by Lanczos vector direct superposition method,and its predicted modes and natural frequencies are compared with experimental results,which verifies the accuracy and reliability of the established finite element model.On this basis,the effects of various key structural or material factors on the natural frequencies of the flexible road wheel are studied using orthogonal experimental design method.Besides,the vibration modal characteristics of the flexible road wheel are also compared with those of the rigid road wheel.The research results provide a theoretical basis for the vibration and noise reduction of flexible road wheel.
文摘A new process for preparing poly(phenylene sulfide amide, PPSA), which is by reaction of sulfur instead of sodium sulfide as S source with dichlorobenzamide (DCBA) and alkali in polar organic solvent at the atmospheric pressure (called sulfur solution route), is reported in the present paper. The influences of polymerization time, molar ratio of precursors, catalyst and solvent upon the polymer were investigated. To seek the best parameters of polymerization, orthogonal design was employed in the experiments. The results indicate that the molar ratio of precursors is the most significant effect on both of viscosity and yield of the polymer. The suitable parameters for preparing the related polymer are presented. The polymer was characterized by IRspectrum, 1HNMRspectrum and Raman spectrum, etc.
