In this study, we report on the degradation of microcystin-LR （MC-LR） by gas- liquid interracial discharge plasma. The influences of operation parameters such as average input voltage, electrode distance and gas flo...In this study, we report on the degradation of microcystin-LR （MC-LR） by gas- liquid interracial discharge plasma. The influences of operation parameters such as average input voltage, electrode distance and gas flow rate are investigated. Experimental results indicate that the input voltage and gas flow rate have positive influences on MC-LR degradation, while the electrode distance has a negative one. After 6 min discharge with 25 kV average input voltage and 60 L/h air aerati by discharge both in on, the degradation rate of MC-LR achieves 75.3%. distilled water and MC-LR solution are measured H202 and 03 generated Moreover, an emission spectroscopy is used as an indicator of the processes that take place on the gas-liquid boundary and inside plasma. Varied types of radicals （O, .OH, CO, 03, etc.） are proved to be present in the gas phase during gas-liquid interfacial discharge.展开更多
为了实现对微囊藻细胞中微囊藻毒素LR(Microcystin LR)的准确定量,建立了铜绿微囊藻中微囊藻毒素LR的超高效液相色谱三重四级杆质谱联用(UPLC-MS/MS)分析方法。将铜绿微囊藻干藻粉复溶在50 m L 0.1%三氟乙酸水溶液中,-55℃/37℃反复冻融...为了实现对微囊藻细胞中微囊藻毒素LR(Microcystin LR)的准确定量,建立了铜绿微囊藻中微囊藻毒素LR的超高效液相色谱三重四级杆质谱联用(UPLC-MS/MS)分析方法。将铜绿微囊藻干藻粉复溶在50 m L 0.1%三氟乙酸水溶液中,-55℃/37℃反复冻融3次,HLB固相萃取小柱浓缩和净化。采用ACQUITY UPLC CSH^(TM)C_(18)色谱柱,以0.1%甲酸-水和0.1%甲酸-乙腈为流动相,梯度洗脱分离,UPLC-MS/MS外标法定量分析。微囊藻毒素LR在5 ng/m L^150 ng/m L范围内线性良好,相关系数大于0.995,方法检出限为0.28 ng/m L,加标回收率在98.5%~104%,日内重复性和日间重复性分别为3.5%和5.6%(n=6)。该方法操作简便,重复性好,对微囊藻细胞中微囊藻毒素MC-LR的定量准确可靠。展开更多
基金supported by National Natural Science Foundation of China(Nos.21006017 and 20906079)Zhejiang Provincial Natural Science Foundation of China(Grant Y5100356)
文摘In this study, we report on the degradation of microcystin-LR （MC-LR） by gas- liquid interracial discharge plasma. The influences of operation parameters such as average input voltage, electrode distance and gas flow rate are investigated. Experimental results indicate that the input voltage and gas flow rate have positive influences on MC-LR degradation, while the electrode distance has a negative one. After 6 min discharge with 25 kV average input voltage and 60 L/h air aerati by discharge both in on, the degradation rate of MC-LR achieves 75.3%. distilled water and MC-LR solution are measured H202 and 03 generated Moreover, an emission spectroscopy is used as an indicator of the processes that take place on the gas-liquid boundary and inside plasma. Varied types of radicals （O, .OH, CO, 03, etc.） are proved to be present in the gas phase during gas-liquid interfacial discharge.
