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基于RhoA/ROCK通路研究复方中药对腹水肉鸡心肌损伤的作用
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作者 康杰 王慧敏 +1 位作者 邓瑞强 段智变 《特产研究》 2025年第3期44-48,54,共6页
为分析RhoA/ROCK信号通路在肉鸡腹水综合征(Ascites syndrome, AS)心肌损伤中的作用及复方中药的防治机制,本研究选取100羽健康的罗斯肉鸡(7日龄)并随机分成5组(每组20只),即基础饲粮饲养对照组,多因素法构建的AS模型组,复方中药高、低... 为分析RhoA/ROCK信号通路在肉鸡腹水综合征(Ascites syndrome, AS)心肌损伤中的作用及复方中药的防治机制,本研究选取100羽健康的罗斯肉鸡(7日龄)并随机分成5组(每组20只),即基础饲粮饲养对照组,多因素法构建的AS模型组,复方中药高、低剂量治疗组以及L-精氨酸治疗组。观察临床症状和剖检变化,并通过HE染色和Masson染色分别观察35日龄肉鸡心脏组织结构变化和胶原纤维沉积;计算肉鸡腹水心脏指数(Ascites heart index, AHI);利用实时荧光定量PCR检测心脏组织MYPT1 mRNA的转录情况,ELISA法检测心脏组织RhoA/ROCK信号通路相关蛋白的表达。结果表明,与空白对照组相比,模型组肉鸡腹部膨大,触压有波动感,腹腔内呈现大量淡黄色液体,右心肥大,心脏组织结构排列紊乱,胶原纤维大量沉积,而且M组AHI显著升高(P <0.01);荧光定量PCR结果表明,心脏组织MYPT1 mRNA转录显著升高(P <0.01)。ELISA结果表明,RhoA、ROCK、P-MLC表达显著升高(P <0.01),而MLCP表达显著降低(P <0.01);与AS组相比,复方中药治疗组的肉鸡相关临床症状及上述指标明显改善。本研究发现,RhoA/ROCK通路介导的MLC磷酸化参与了AS中心肌损伤的发生,复方中药水提液可通过调节RhoA/ROCK抑制心肌损伤来有效防治AS。 展开更多
关键词 复方中药 肉鸡腹水综合征 心肌损伤 RhoA/rock通路
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miR-202-5p通过靶向抑制ROCK1表达减轻急性呼吸窘迫综合征新生大鼠肺损伤
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作者 杨英阁 张晋雷 +2 位作者 孙岩 陈升 梁正 《中国医科大学学报》 北大核心 2025年第9期814-820,共7页
目的 探讨miR-202-5p对急性呼吸窘迫综合征(ARDS)新生大鼠肺损伤的保护作用及其机制。方法 60只新生SD大鼠随机分为对照组(腹腔注射生理盐水)、ARDS组[腹腔注射脂多糖(4 mg/kg)建立ARDS模型]、ARDS+miR-NC组[ARDS大鼠尾静脉注射miR-NC(2... 目的 探讨miR-202-5p对急性呼吸窘迫综合征(ARDS)新生大鼠肺损伤的保护作用及其机制。方法 60只新生SD大鼠随机分为对照组(腹腔注射生理盐水)、ARDS组[腹腔注射脂多糖(4 mg/kg)建立ARDS模型]、ARDS+miR-NC组[ARDS大鼠尾静脉注射miR-NC(2 mg/kg),1次/4 h,连续干预12 h]、ARDS+miR-202-5p组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h]、ARDS+miR-202-5p+ov-NC组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h;脂多糖注射前3 d尾静脉注射6×10~8 PFU过表达对照腺病毒]和ARDS+miR-202-5p+ov-ROCK1组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h;脂多糖注射前3 d尾静脉注射6×10^(8) PFU过表达ROCK1腺病毒],每组10只。实时定量PCR检测大鼠肺组织中miR-202-5p和ROCK1 mRNA表达;HE染色观察大鼠肺组织病理损伤;计算大鼠肺组织湿重/干重比值来评价大鼠肺水肿程度;Western blotting检测大鼠肺组织中ROCK1、TLR4、p-NF-κB p65和p-IκBα蛋白表达。双萤光素酶报告基因实验验证miR-202-5p与ROCK1的关系。结果 miR-202-5p在ARDS大鼠肺组织中表达下调,过表达miR-202-5p可减轻ARDS大鼠肺组织病理损伤和肺水肿。ROCK1 mRNA在ARDS大鼠肺组织中表达上调;过表达miR-202-5p降低ARDS大鼠肺组织中ROCK1表达,而过表达ROCK1逆转了过表达miR-202-5p对ARDS新生大鼠肺损伤的保护作用。过表达miR-202-5p抑制ARDS大鼠肺组织中TLR4、p-NF-κB p65和p-IκBα蛋白表达,过表达ROCK1逆转此作用。双萤光素酶报告基因实验结果显示miR-202-5p靶向结合ROCK1 mRNA的3’UTR。结论 miR-202-5p通过靶向抑制ROCK1表达减轻ARDS大鼠肺损伤,其机制可能与抑制ROCK1介导的TLR4/NF-κB信号通路激活有关。 展开更多
关键词 急性呼吸窘迫综合征 miR-202-5p rock1 TLR4/NF-κB信号通路 肺损伤
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毛兰素通过调节HMGB1/RAGE-RhoA/ROCK1信号通路缓解特应性皮炎
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作者 徐可欣 王丹丹 +5 位作者 金泓宇 杜月 李莉 宋艺兰 延光海 李良昌 《中国比较医学杂志》 北大核心 2025年第4期11-20,共10页
目的探究毛兰素(Erianin)在特应性皮炎(atopic dermatitis,AD)中的作用及其在高迁移率族蛋白1(high mobility group box-1,HMGB1)/晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)-Ras同源基因家族成员A(Ra... 目的探究毛兰素(Erianin)在特应性皮炎(atopic dermatitis,AD)中的作用及其在高迁移率族蛋白1(high mobility group box-1,HMGB1)/晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)-Ras同源基因家族成员A(Ras homolog gene family member A,RhoA)/Rho关联含卷曲螺旋结合蛋白激酶1(recombinant Rho associated coiled coil containing protein kinase 1,ROCK1)信号通路中的调控机制。方法1-氯-2,4-二硝基苯(1-Chloro-2,4-dinitrobenzene,DNCB)诱导BALB/c小鼠作为AD的模型,测量小鼠的皮肤厚度、脾和淋巴结的重量。甲苯胺蓝和HE染色检测小鼠的背部皮肤和耳朵的病理改变;ELISA检测炎症因子水平;肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)刺激HaCaT细胞建立AD体外模型;采用流式细胞术检测细胞活性氧(reactive oxygen species,ROS);免疫荧光法检测线粒体活性氧(mitochondrion reactive oxygen species,mtROS);TUNEL检测细胞凋亡情况;免疫蛋白印迹法检测HMGB1、RAGE、RhoA、ROCK1蛋白表达情况。结果在体内实验中毛兰素抑制皮肤厚度的增加,减轻脾和淋巴结重量,改善炎症细胞的浸润和肥大细胞脱颗粒,降低炎症因子水平(P<0.05)。在体外实验中,毛兰素减少TNF-α诱导的HaCaT细胞ROS、mtROS的产生(P<0.01)。毛兰素治疗后HMGB1、RAGE、RhoA及ROCK1的蛋白表达量下降(P<0.01);使用RAGE特异性阻断剂(TFA)处理r-HMGB1刺激的HaCaT细胞后,HMGB1的表达没有发生变化,RAGE、RhoA及ROCK1表达减少(P<0.01);在Rho激酶抑制剂Y-27632+r-HMGB1组中,除RAGE的表达没有降低,其余结果与TFA+r-HMGB1组相近。结论毛兰素可能通过调节HMGB1/RAGE-RhoA/ROCK1信号通路缓解特应性皮炎。 展开更多
关键词 毛兰素 特应性皮炎 HMGB1 RAGE RHOA rock1
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Strength criterion for crystalline rocks considering grain size effect and tensile-compressive strength ratio
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作者 ZHANG Cheng-han JI Hong-guang +3 位作者 JIANG Peng YOU Shuang GENG Qian-cheng JIAO Chen-jiang 《Journal of Central South University》 SCIE EI CAS CSCD 2024年第7期2365-2378,共14页
The macroscopic mechanical properties of rocks are significantly influenced by their microstructure.As a material bonded by mineral grains,the grain morphology of crystalline rock is the primary factor influencing the... The macroscopic mechanical properties of rocks are significantly influenced by their microstructure.As a material bonded by mineral grains,the grain morphology of crystalline rock is the primary factor influencing the strength.However,most strength criteria neglect the strength variations caused by different grain characteristics in rocks.Furthermore,the traditional linear criteria tend to overestimate tensile strength and exhibit apex singularity.To address these shortcomings,a piecewise strength criterion that considers the grain size effect has been proposed.A part of an ellipse was employed to construct the envelope of the tensive-shear region on the meridian plane,to accurately reproduce the low tensile-compressive strength ratio.Based on the analysis of experimental data,both linear and exponential modification functions that account for grain size effects were integrated into the proposed criterion.The corresponding finite element algorithm has been implemented.The accuracy and applicability of the proposed criterion were validated by comparing with the experimental data. 展开更多
关键词 crystalline rock grain size effect strength criterion tensile-compressive strength ratio finite element algorithm
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ROCK-siRNA转染对AngⅡ诱导的内皮细胞衰老及内皮细胞微粒的影响
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作者 王恺 王嫣 +3 位作者 陈天琪 彭芳 周慧 史琴 《细胞与分子免疫学杂志》 北大核心 2025年第9期778-783,共6页
目的探讨转染ROCK-siRNA对血管紧张素Ⅱ(AngⅡ)诱导的内皮细胞衰老及内皮细胞微粒(EMP)的影响。方法使用AngⅡ(1.0μmol/L)诱导人脐静脉内皮细胞(HUVEC)制备细胞衰老模型,并用ROCK干扰RNA(ROCK-siRNA)转染细胞,分为对照组、模型组、转... 目的探讨转染ROCK-siRNA对血管紧张素Ⅱ(AngⅡ)诱导的内皮细胞衰老及内皮细胞微粒(EMP)的影响。方法使用AngⅡ(1.0μmol/L)诱导人脐静脉内皮细胞(HUVEC)制备细胞衰老模型,并用ROCK干扰RNA(ROCK-siRNA)转染细胞,分为对照组、模型组、转染阴性对照组(AngⅡ联合NC-siRNA)、ROCK-siRNA转染组(AngⅡ联合ROCK-siRNA)。采用SA-β-Gal法鉴定细胞衰老程度,流式细胞术检测培养液上清中EMP的数量及细胞内活性氧(ROS)水平,Western blot法检测各组细胞中沉默信息调节因子2相关酶1(SIRT1)、p53蛋白的表达。结果ROCK-siRNA转染内皮细胞后,可明显减少AngⅡ诱导的衰老的内皮细胞数量,抑制衰老细胞释放CD31^(+)EMP及ROS生成,同时可上调SIRT1蛋白表达、下调p53蛋白表达水平。结论沉默ROCK可抑制EMP释放,减少ROS生成,调控SIRT1、p53表达,延缓AngⅡ诱导的内皮细胞衰老。 展开更多
关键词 内皮细胞微粒(EMP) 衰老 rock 血管紧张素Ⅱ(AngⅡ)
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基于Rho A/ROCK通路探索鬼箭羽醇提物对肝纤维化小鼠的影响
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作者 马敏 曾华华 +2 位作者 孙宇杰 胡嘉敏 黄德斌 《中华中医药学刊》 北大核心 2025年第8期131-136,I0047,共7页
目的基于Rho A/ROCK信号通路探究鬼箭羽醇提物(Ethanolic extracts of euonymus alatus,EEEA)对四氯化碳(CCl_(4))诱导的肝纤维化(Hepatic fibrosis,HF)模型小鼠的影响。方法将50只C57BL/6小鼠随机分为空白组(Normal group,NG)、模型组(... 目的基于Rho A/ROCK信号通路探究鬼箭羽醇提物(Ethanolic extracts of euonymus alatus,EEEA)对四氯化碳(CCl_(4))诱导的肝纤维化(Hepatic fibrosis,HF)模型小鼠的影响。方法将50只C57BL/6小鼠随机分为空白组(Normal group,NG)、模型组(Model group,MG)、鬼箭羽低剂量组(EA-L)、鬼箭羽中剂量组(EA-M)、鬼箭羽高剂量组(EA-H),每组10只。除NG外,其余各组小鼠腹腔注射含有25%CCl_(4)的橄榄油混悬液造模。造模的同时,NG、MG以双蒸水灌胃,其余3组按对应剂量灌胃:EA-L、EA-M、EA-H 3个剂量分别为2、4、8 g/kg(相当于含生药量)。6周后取材,收集小鼠血清和肝组织,采用谷草转氨酶(Glutamic oxalacetic transaminase,AST)、谷丙转氨酶(Glutamate pyruvate transaminase,ALT)测试盒检测血清中肝功能指标。ELISA法检测血清中肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)和白细胞介素6(Interleukin-6,IL-6)含量,相同方式检测肝组织羟脯氨酸(Hydroxyproline,Hyp)、转换生长因子β1(Transforming growth factor-β1,TGF-β1)的含量。HE和Masson染色分析小鼠肝脏的损伤和胶原沉积情况。Western blot法检测肝组织中α平滑肌动蛋白(α-smooth muscle actin,α-SMA)、Ⅰ型胶原蛋白(TypeⅠCollagen,CollagenⅠ)以及信号通路Rho A/ROCK相关蛋白的相对表达量。结果与MG相比,EA-M和EA-H血清ALT、AST、TNF-α、IL-6呈下降趋势;与MG相比,鬼箭羽药物组肝组织Hyp、TGF-β1的含量不同程度下降(P<0.05,P<0.01)。鬼箭羽药物组的肝形态结构、细胞炎性浸润、纤维变化及胶原沉积呈剂量依赖性优于MG;与MG相比,EA-L、EA-M和EA-H的α-SMA、CollagenⅠ及Rho A/ROCK信号通路相关蛋白表达水平下降(P<0.05,P<0.01)。与MG相比,EA-L、EA-M和EA-H的α-SMA、CollagenⅠ及Rho A/ROCK信号通路相关蛋白mRNA表达下降(P<0.05,P<0.01)。结论鬼箭羽醇提物具有明显的降酶护肝、抗肝纤维化的作用,而抑制Rho A/ROCK信号通路是其抗肝纤维化的作用机制之一。 展开更多
关键词 鬼箭羽醇提物 肝纤维化 Rho A/rock信号通路
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HMGB1对复发性流产模型子宫内膜容受性及RhoA/ROCK2信号通路的影响
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作者 王娇 李素叶 +4 位作者 焦艳 王磊 崔家璐 冀雪霞 张聪祎 《医学研究与战创伤救治》 北大核心 2025年第6期575-581,共7页
目的探讨高迁移率群盒蛋白1(HMGB1)在复发性流产(RSA)模型中对子宫内膜容受性的影响,以及其与RhoA/ROCK2信号通路之间的关系。方法雌性CBA/J小鼠按照随机数字表法分成5组:Con组(正常妊娠小鼠),RSA组(复发性流产模型小鼠)、sh-HMGB1组(RS... 目的探讨高迁移率群盒蛋白1(HMGB1)在复发性流产(RSA)模型中对子宫内膜容受性的影响,以及其与RhoA/ROCK2信号通路之间的关系。方法雌性CBA/J小鼠按照随机数字表法分成5组:Con组(正常妊娠小鼠),RSA组(复发性流产模型小鼠)、sh-HMGB1组(RSA小鼠体内转染sh-HMGB1组)、sh-HMGB1+LPA组(RSA小鼠体内转染sh-HMGB1组并注射RhoA激活剂溶血磷脂酸)、C3组(RSA小鼠注射Rho A抑制剂C3外酶)。HE染色进行组织病理学分析。ELISA试剂盒检测子宫内膜组织炎症因子水平。检测子宫内膜厚度与腺体数目。TUNEL检测组织细胞凋亡。qRT-PCR和蛋白免疫印迹检测HMGB1、HOXa10、VEGF、Caspase-9、Caspase-3、RhoA和ROCK2的表达水平。结果与Con组相比,RSA组的TNF-α、IFN-γ、IL-6水平、TUNEL阳性细胞减少(P<0.05),RhoA、ROCK2的m RNA和蛋白表达水平显著增加(P<0.05),平均胚胎着床点、子宫内膜厚度、腺体数目数、HOXa10、VEGF、Caspase-9和Caspase-3的mRNA和蛋白表达水平显著降低(P<0.05)。与RSA组相比,sh-HMGB1组和C3组的TNF-α、IFN-γ、IL-6水平、TUNEL阳性细胞减少(P<0.05),RhoA、ROCK2的m RNA和蛋白表达水平显著降低(P<0.05),平均胚胎着床点、子宫内膜厚度、腺体数目数、HOXa10、VEGF、Caspase-9和Caspase-3的m RNA和蛋白表达水平显著增加(P<0.05)。与sh-HMGB1组相比,sh-HMGB1+LPA组TNF-α、IFN-γ、IL-6水平、TUNEL阳性细胞减少(P<0.05),RhoA、ROCK2的m RNA和蛋白表达水平显著增加(P<0.05),平均胚胎着床点、子宫内膜厚度、腺体数目数、HOXa10、VEGF、Caspase-9和Caspase-3的m RNA和蛋白表达水平显著降低(P<0.05)。结论HMGB1在RSA模型中通过促进炎症反应,抑制子宫内膜的细胞凋亡对子宫内膜容受性产生负面影响,其机制可能与Rho A/ROCK2信号通路的异常激活有关。 展开更多
关键词 高迁移率群盒蛋白1 复发性流产 子宫内膜容受性 RhoA/rock2
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Strength and failure characteristics of hard rock containing a single structural plane under varied loading angles : A true triaxial investigation
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作者 XU Huai-sheng LI Shao-jun +3 位作者 XU Ding-ping LIU Xu-feng FENG Guang-liang WANG Zhao-feng 《Journal of Central South University》 2025年第5期1903-1921,共19页
The spatial relationship between structural planes and principal stresses significantly affects the mechanical properties of deep hard rock.This paper examines the effect of the loading angle under true triaxial compr... The spatial relationship between structural planes and principal stresses significantly affects the mechanical properties of deep hard rock.This paper examines the effect of the loading angle under true triaxial compression.While previous studies focused on the angleβbetween the maximum principal stress and the structural plane,the role of angleω,between the intermediate principal stress and the structural plane,is often overlooked.Utilizing artificially prefabricated granite specimens with a single non-penetrating structural plane,we set the loading angleβto range from 0°to 90°across seven groups,and assignedωvalues of 0°and 90°in two separate groups.The results show that the peak strength is negatively correlated withβup to 45°,beyond which it tends to stabilize.The angleωexerts a strengthening effect on the peak strength.Deformation mainly occurs post-peak,with the strain values ε_(1) and ε_(3) reaching levels 2−3 times higher than those in intact rock.The structural plane significantly influences failure mode whenω=0°,while failure localizes near the σ_(3) surface of the specimens whenω=90°.The findings enhance data on structural plane rocks under triaxial compression and inform theoretical research,excavation,and support design of rock structures. 展开更多
关键词 true triaxial compression hard rock structural plane loading angle STRENGTH failure characteristics
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Stress gradient analytic solution and reasonable support prestress of roadway surrounding rock based on unified strength criterion:A case study
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作者 JING Suo-lin WEN Zhi-jie +2 位作者 ZUO Yu-jun LI Qiu-ju HAO Peng 《Journal of Central South University》 2025年第2期449-468,共20页
The stress gradient of surrounding rock and reasonable prestress of support are the keys to ensuring the stability of roadways.The elastic-plastic analytical solution for surrounding rock was derived based on unified ... The stress gradient of surrounding rock and reasonable prestress of support are the keys to ensuring the stability of roadways.The elastic-plastic analytical solution for surrounding rock was derived based on unified strength theory.A model for solving the stress gradient of the surrounding rock with the intermediate principal stress parameter b was established.The correctness and applicability of the solution for the stress gradient in the roadway surrounding rock was verified via multiple methods.Furthermore,the laws of stress,displacement,and the plastic zone of the surrounding rock with different b values and prestresses were revealed.As b increases,the stress gradient in the plastic zone increases,and the displacement and plastic zone radius decrease.As the prestress increases,the peak stress shifts toward the sidewalls,and the stress and stress gradient increments decrease.In addition,the displacement increment and plastic zone increment were proposed to characterize the support effect.The balance point of the plastic zone area appears before that of the displacement zone.The relationship between the stress gradient compensation coefficient and the prestress is obtained.This study provides a research method and idea for determining the reasonable prestress of support in roadways. 展开更多
关键词 PRESTRESS support compensation surrounding rock damage stress gradient analytic solution unified strength theory
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Research on damage characteristics and constitutive model of rock mass under true triaxial cyclic loading based on acoustic emission
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作者 LI Ying-ming FAN Chao-tao +6 位作者 DONG Chun-liang ZHAO Guang-ming MENG Xiang-rui WANG Xiang-jun SHI Wen-qiu WU Xin-wen GAO Jiang-huai 《Journal of Central South University》 2025年第5期1938-1954,共17页
Aiming at the problem of deep surrounding rock instability induced by roadway excavation or mining disturbance,the true triaxial loading system was used to conduct graded cyclic maximum principal stress σ_(1) and int... Aiming at the problem of deep surrounding rock instability induced by roadway excavation or mining disturbance,the true triaxial loading system was used to conduct graded cyclic maximum principal stress σ_(1) and intermediate principal stress σ_(2) tests on sandstone to simulate the effect of mining stress in actual underground engineering.The influences of each principal stress cycle on the mechanical properties,acoustic emission(AE)characteristics,and fracture characteristics of sandstone were analyzed.The damage characteristics of sandstone under true triaxial cyclic loading were studied.Furthermore,the damage constitutive model of rock mass under true triaxial cyclic loading was established based on AE cumulative ringing count.The quantitative investigation was conducted on cumulative-damage changes in circulating sandstone,which elucidated the mechanism of damage deterioration in sandstone subjected to true triaxial cyclic loading.The results show that the influence of the graded cycleσ_(1) on limit maximum principal strain ɛ_(1max) and limit minimum principal strainɛ_(3max) was significantly greater than that of the limit intermediate principal strain ɛ_(2max).Graded cycleσ_(2) had a greater impact onɛ_(2max) and a smaller impact onɛ_(3max).The elasticity modulus of sandstone decreased exponentially with the increased cyclic load amplitude,while the Poisson ratio increased linearly.b of AE showed a trend of increasing,decreasing,slightly fluctuating,and finally decreasing during cyclingσ_(1).b showed a trend of slight fluctuation,large fluctuation,and finally increase during cyclingσ_(2).Sandstone specimens experienced mainly tensile failure,tensile-shear composite failure,and mainly shear failure with increased initialσ_(2) orσ_(3).This was determined by analyzing the rise angle-average frequency of the AE parameter,corresponding to the rock specimens from splitting failure to shear failure.Besides,the mechanical damage behavior of sandstone under true triaxial cyclic loading could be well described by the established constitutive model.At the same time,it was found that the sandstone damage variable decreased with increasedσ_(2) during cyclingσ_(1).The damage variable decreased first and then increased with increasedσ_(3) during cyclingσ_(2). 展开更多
关键词 rock mechanics true triaxial cyclic principal stress acoustic emission constitutive model damage variable
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Anisotropic strength and deformation of irregular columnar jointed rock masses under triaxial stress
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作者 QUE Xiang-cheng ZHU Zhen-de +1 位作者 NIU Zi-hao ZHU Shu 《Journal of Central South University》 2025年第2期643-655,共13页
The special columnar jointed structure endows rocks with significant anisotropy,accurately grasping the strength and deformation properties of a columnar jointed rock mass(CJRM)under complex geological conditions is c... The special columnar jointed structure endows rocks with significant anisotropy,accurately grasping the strength and deformation properties of a columnar jointed rock mass(CJRM)under complex geological conditions is crucial for related engineering safety.Combined with the irregular jointed networks observed in the field,artificial irregular CJRM(ICJRM)samples with various inclination angles were prepared for triaxial tests.The results showed that the increase in confining pressure can enhance the ability of the ICJRM to resist deformation and failure,and reduce the deformation and strength anisotropic degrees.Considering the field stress situation,the engineering parts with an inclination angle of 30°−45°need to be taken seriously.Four typical failure modes were identified,and the sample with an inclination angle of 15°showed the same failure behavior as the field CJRM.Traditional and improved joint factor methods were used to establish empirical relationships for predicting the strength and deformation of CJRM under triaxial stress.Since the improved joint factor method can reflect the unique structure of CJRM,the predictive ability of the empirical relationship based on the improved method is better than that based on the traditional joint factor method. 展开更多
关键词 irregular columnar jointed rock mass triaxial stress STRENGTH DEFORMATION anisotropic mechanical property empirical relation
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高寒草甸有机碳结构和成分组成及Rock-Eval热解研究
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作者 张永利 冉勇 《地球化学》 北大核心 2025年第3期426-435,共10页
近年来对高寒草甸有机碳结构和成分的研究开始增多,但未见将先进13C固态核磁共振(SS-NMR)技术和Rock-Eval热解技术同时应用于高寒草甸有机碳表征的研究。本研究采用化学分离法将高寒草甸样品连续分为原始(OS)级分、去碳酸盐(DC)级分、去... 近年来对高寒草甸有机碳结构和成分的研究开始增多,但未见将先进13C固态核磁共振(SS-NMR)技术和Rock-Eval热解技术同时应用于高寒草甸有机碳表征的研究。本研究采用化学分离法将高寒草甸样品连续分为原始(OS)级分、去碳酸盐(DC)级分、去矿(DM)级分、去类脂(DF)级分和酸不可水解(NHC)级分。元素分析结果显示,OS、DC、DM、DF和NHC级分的有机碳含量分别为7.7%、8.8%、41.6%、38.4%和54.2%,稳定C同位素(δ^(13)C)值分别为−23.7‰、−25.0‰、−25.4‰、−25.1‰和−26.5‰。稳定C、N同位素和贝叶斯混合模型分析结果表明,高寒草甸有机碳的C3植物源和微生物源分别为46.5%和53.5%。13C SS-NMR分析结果表明,高寒草甸有机碳的结构组成中,脂肪碳和芳香碳分别占总有机碳的55.4%和34.5%。其中,脂肪碳主要包括氧烷基碳(O-alkyl C,23.8%)、烷基碳(Alkyl C,19.0%)和甲氧基碳/N-烷基碳(OCH3/NCH,12.6%);芳香碳主要包括质子化芳香碳(Arom C-H,15.6%)和非质子化芳香碳(Arom C-C,13.1%)。生物大分子成分组成分析结果表明,不稳定的碳水化合物和蛋白质是高寒草甸的主要成分,非质子化木炭和具有Alkyl C结构的孢粉素是稳定有机碳的重要成分。Rock-Eval热解分析结果表明,氧指数(OI)与极性碳(Polar C)含量、碳水化合物+蛋白质+木质素、Alkyl C/O-alkyl C值呈显著相关关系(R^(2)≥0.96,P<0.01),说明OI可表征高寒草甸不稳定有机碳的分解潜力和腐殖化状态;S2与稳定生物源的Alkyl C和孢粉素呈显著正相关关系(R^(2)≥0.96,P<0.01),残留有机碳(RC)与非质子化木炭呈显著正相关关系(R^(2)=0.91,P<0.05),表明S2和RC可代表高寒草甸稳定有机碳的结构和成分组成。 展开更多
关键词 脂肪碳 芳香碳 同位素 固态核磁共振(SS-NMR) rock-EVAL
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An improved limit equilibrium method for rock slope stability analysis under stress-based calculation mode for slip surface
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作者 DENG Dong-ping ZHANG Dian +1 位作者 PENG Yi-hang CHEN Hao-yu 《Journal of Central South University》 2025年第1期262-287,共26页
This study proposes an alternative calculation mode for stresses on the slip surface(SS).The calculation of the normal stress(NS)on the SS involves examining its composition and expanding its unknown using the Taylor ... This study proposes an alternative calculation mode for stresses on the slip surface(SS).The calculation of the normal stress(NS)on the SS involves examining its composition and expanding its unknown using the Taylor series.This expansion enables the reasonable construction of a function describing the NS on the SS.Additionally,by directly incorporating the nonlinear Generalized Hoke-Brown(GHB)strength criterion and utilizing the slope factor of safety(FOS)definition,a function of the shear stress on the SS is derived.This function considers the mutual feedback mechanism between the NS and strength parameters of the SS.The stress constraints conditions are then introduced at both ends of the SS based on the spatial stress relation of one point.Determining the slope FOS and stress solution for the SS involves considering the mechanical equilibrium conditions and the stress constraint conditions satisfied by the sliding body.The proposed approach successfully simulates the tension-shear stress zone near the slope top and provides an intuitive description of the concentration effect of compression-shear stress of the SS near the slope toe.Furthermore,compared to other methods,the present method demonstrates superior processing capabilities for the embedded nonlinear GHB strength criterion. 展开更多
关键词 stability of rock slope nonlinear GHB strength criterion limit equilibrium method stress function on slip surface stress constraint conditions at both ends of slip surface
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Rocks高性能计算集群的建立和管理 被引量:4
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作者 张予倩 周健 +1 位作者 翁红明 韩静 《实验室研究与探索》 CAS 2006年第4期450-453,共4页
论述了用Rocks集群操作系统来搭建和管理具有高性能计算能力的PC集群的方法和过程,并以目前流行的几种支持并行计算的第一性原理软件包ABINIT及VASP为例,详细说明了Sun Grid Engine(SGE)对用户计算任务管理的方法和因此而显示出的优越性... 论述了用Rocks集群操作系统来搭建和管理具有高性能计算能力的PC集群的方法和过程,并以目前流行的几种支持并行计算的第一性原理软件包ABINIT及VASP为例,详细说明了Sun Grid Engine(SGE)对用户计算任务管理的方法和因此而显示出的优越性,同时还探讨了各种软件在集群上的加速比问题。 展开更多
关键词 高性能计算集群 MPI并行程序 rocks SUN GRID ENGINE
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基于Rocks的高性能集群平台搭建与应用 被引量:4
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作者 张洋 陈文波 李廉 《计算机工程与科学》 CSCD 2008年第11期137-139,143,共4页
高性能计算是实现大规模并行计算的有效途径,本文论述了如何构建一个高性能集群,介绍了集群安装工具——Rocks ,分析Rocks安装机制、特点以及Rocks如何管理集群,利用Rocks搭建了一个基于Linux的高性能集群平台,并在该平台上实现了量子... 高性能计算是实现大规模并行计算的有效途径,本文论述了如何构建一个高性能集群,介绍了集群安装工具——Rocks ,分析Rocks安装机制、特点以及Rocks如何管理集群,利用Rocks搭建了一个基于Linux的高性能集群平台,并在该平台上实现了量子化学软件Gaussian的应用,进一步测试了Rocks系统的性能。 展开更多
关键词 高性能集群rocks ROLL
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贝母素乙调节RhoA/ROCK信号通路对膝骨关节炎大鼠软骨损伤的影响 被引量:1
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作者 彭博文 冯立 覃剑 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第7期980-984,共5页
目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组... 目的 探讨贝母素乙调节Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)信号通路对膝骨关节炎(knee osteoarthritis, KOA)大鼠软骨损伤的影响。方法 以碘乙酸钠(MIA)法制备KOA大鼠模型,随机分为模型组、贝母素乙(3.5 mg/kg)组、贝母素乙(3.5 mg/kg)+LPA(RhoA激活剂,1 mg/kg)组,每组10只,另选10只大鼠为对照组。机械刺激法和热辐射法检测大鼠膝关节疼痛症状;检测大鼠关节肿胀度、膝关节宽度并以步态评分评测其关节功能;透射电镜检测大鼠膝关节软骨细胞超微结构;TUNEL染色检测大鼠关节软骨细胞凋亡比;酶联免疫吸附(ELISA)法检测大鼠血清和关节液促炎因子白细胞介素(IL)-18、单核细胞趋化蛋白1(MCP-1)水平;免疫印迹检测各组大鼠关节软骨组织凋亡(Cleaved caspase-3、Bax)和RhoA/ROCK通路相关蛋白表达。结果 贝母素乙可降低KOA大鼠关节肿胀度、膝关节宽度、步态评分、软骨细胞凋亡比、血清及关节液IL-18、MCP-1水平、软骨组织Cleaved caspase-3、Bax、RhoA、ROCK1、ROCK1蛋白表达,升高机械痛阈值、热痛阈值(P<0.05);LPA可减弱贝母素乙对KOA大鼠关节软骨损伤的改善作用。结论 贝母素乙可通过抑制RhoA/ROCK信号激活而抑制KOA大鼠关节炎症反应和关节软骨细胞凋亡,减轻软骨损伤。 展开更多
关键词 贝母素乙 RhoA/rock 膝骨关节炎 软骨损伤 信号通路
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SLC9A3-AS1调控miR-148a-3p/ROCK1信号轴影响肾癌细胞生物学功能 被引量:4
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作者 向威 吕磊 +2 位作者 郑福鑫 章传华 袁敬东 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第2期161-167,共7页
目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9... 目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9A3-AS1在ccRCC组织中的表达水平;采用实时荧光定量聚合酶链反应(qRT-PCR)检测SLC9A3-AS1在不同肾癌细胞系、24例ccRCC组织与癌旁正常肾脏组织中的表达水平;应用细胞增殖/毒性检测试剂盒(cell counting kit-8,CCK-8)和Transwell小室迁移实验检测敲低SLC9A3-AS1表达对肾癌细胞增殖与迁移的影响;应用蛋白质免疫印迹法检测增殖与迁移相关信号通路蛋白的表达水平;采用双荧光素酶报告基因实验验证SLC9A3-AS1与miR-148a-3p/ROCK1轴的靶向调控关系。结果GEPIA2软件分析结果显示,相较正常肾脏组织,SLC9A3-AS1在肾透明细胞癌组织中表达显著上调(P<0.01)。qRT-PCR结果显示,相较癌旁正常肾脏组织,SLC9A3-AS1在24例ccRCC组织中表达显著上调(P<0.01);相较永生化肾小管上皮细胞,SLC9A3-AS1在4种肾癌细胞系中表达均显著上调,以786-O细胞最为显著(P<0.01)。干扰SLC9A3-AS1表达,可显著抑制786-O细胞的增殖与迁移能力,上调E-cadherin的蛋白表达水平,而下调N-cadherin、MMP2的蛋白表达水平(均P<0.05);过表达miR-148a-3p可显著抑制786-O细胞的增殖与迁移能力(P<0.01)。双荧光素酶报告基因检测结果表明,SLC9A3-AS1可特异性结合miR-148a-3p,后者进一步靶向结合ROCK1 mRNA的3′UTR区域;过表达miR-148a-3p可明显下调ROCK1 mRNA的表达水平,敲低miR-148a-3p表达则产生相反的效应;敲低SLC9A3-AS1表达可显著下调786-O细胞中ROCK1 mRNA与蛋白的表达水平(P<0.01);下调miR-148a-3p表达可部分逆转SLC9A3-AS1沉默对786-O细胞增殖与迁移的抑制作用(P<0.05)。结论SLC9A3-AS1在ccRCC中通过调控miR-148a-3p/ROCK1轴发挥促癌作用,有望成为ccRCC的一个新的分子标志物。 展开更多
关键词 肾透明细胞癌 SLC9A3-AS1 miR-148a-3p rock1
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川芎嗪抑制ROCK的表达降低模拟失重大鼠血管Ca^(2+)敏感性 被引量:1
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作者 王慧平 白晓镯 +4 位作者 赵晶 赵省心 刘朕印 党凯 高云芳 《西北大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第5期919-928,共10页
研究失重条件下血管平滑肌收缩性、Ca^(2+)敏感性及其调控通路RhoA-ROCK蛋白表达的变化,以及川芎嗪干预对其的影响。大鼠尾部悬吊模拟失重,在机体前、后部分别选取颈总动脉和肠系膜上动脉。在模拟失重大鼠颈总动脉中,由苯肾上腺素(PHE)... 研究失重条件下血管平滑肌收缩性、Ca^(2+)敏感性及其调控通路RhoA-ROCK蛋白表达的变化,以及川芎嗪干预对其的影响。大鼠尾部悬吊模拟失重,在机体前、后部分别选取颈总动脉和肠系膜上动脉。在模拟失重大鼠颈总动脉中,由苯肾上腺素(PHE)或KCl诱发的血管收缩性和Ca^(2+)敏感性增强,RhoA激酶2(ROCK II)的表达、肌球蛋白磷酸酶靶亚基1(MYPT1)和肌球蛋白调节轻链(MLC)的磷酸化水平均上升,血管孵育Y-27632(ROCK特异性抑制剂)后可降低以上变化。模拟失重大鼠灌饲川芎嗪亦可降低以上变化。模拟失重后,大鼠肠系膜上动脉的收缩性和Ca^(2+)敏感性、ROCK II的表达、MYPT1和MLC的磷酸化水平降低,血管孵育Y-27632对以上变化无明显作用。模拟失重大鼠灌饲川芎嗪亦对以上变化无明显作用。结果表明,由RhoA-ROCK调控的血管平滑肌Ca^(2+)敏感性的变化可能是失重条件下机体前后部血管收缩性发生区域性重塑的关键因素。川芎嗪可抑制ROCK蛋白的表达,降低血管平滑肌升高的Ca^(2+)敏感性,从而纠正失重条件下机体前部血管收缩性的增强,但对失重条件下机体后部血管收缩性的减弱无恢复作用。 展开更多
关键词 Ca^(2+)敏感性 RhoA-rock 血管收缩性 模拟失重 川芎嗪
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GEOCHEMISTRY OF THE PLIOCENE SHOSHONITIC ROCKS FROM OIYUG BASIN, CENTRAL TIBET 被引量:2
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作者 Zhao Zhidan, Mo Xuanxue, Zhang Shuangquan, Guo Tieying, Zhou Su 《地学前缘》 EI CAS CSCD 2000年第S1期107-108,共2页
Cenozoic volcanism has been well studied in northern, eastern and southwestern Tibet (Coulon et al., 1986; Arnund et al., 1992; Turner et al., 1993, 1996; Deng, 1978, 1998; Miller et al., 1999, Wang et al, 2000). But ... Cenozoic volcanism has been well studied in northern, eastern and southwestern Tibet (Coulon et al., 1986; Arnund et al., 1992; Turner et al., 1993, 1996; Deng, 1978, 1998; Miller et al., 1999, Wang et al, 2000). But the data of the Cenozoic post\|collisional volcanism in central and southern Tibet is limited (Coulon et al., 1986; Turner et al., 1996; Zhang, 1998). These potassic and ultrapotassic intrusive and extrusive rocks are regarded to be a key clue for the deep lithospheric process of the plateau after Indo\|Asian collision. Present here is the preliminary results of the rocks from Oiyug (Wuyu) basin, about 150km northeast to Shigatse. Major and trace elements of the rocks are studied and compared with the ultrapotassic and potassic (shoshonitic) rocks exposed in Lhasa block and Qiangtang terrane.(1) Geologic setting. The magmatic rocks studied are Gazacun formation of the lower part of the Pliocene Oiyug group (N 2 oy ). The cross\|section is situated in Gazacun village of Namling. Gazacun formation is underlain unconformable by the andesitic rocks of Linzizong Group [(K 2—E 2) ln ], and covered by the coarse\|grain layered sandstone and conglomerate of Zongdangcun formation. The Gazacun formation consists of mediate\|acidic volcanics, granite\|porphyry, coal\|bearing clastics, plants and sporo\|pollen. 展开更多
关键词 shoshonitic rocks GEOCHEMISTRY Oiyug BASIN TIBET
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Viscoelasto-plastic rheological experiment under circular increment step load and unload and nonlinear creep model of soft rocks 被引量:23
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作者 赵延林 曹平 +2 位作者 王卫军 万文 刘业科 《Journal of Central South University》 SCIE EI CAS 2009年第3期488-494,共7页
The viscoelastic-plastic creep experiments on soft ore-rock in Jinchuan Mine III were performed under circular increment step load and unload. The experimental data were analyzed according to instantaneous elastic str... The viscoelastic-plastic creep experiments on soft ore-rock in Jinchuan Mine III were performed under circular increment step load and unload. The experimental data were analyzed according to instantaneous elastic strain, visco-elastic strain, instantaneous plastic strain and visco-plastic strain. The result shows that instantaneous deformation modulus tends to increase with the increase of creep stress; soft rocks enhance the ability to resist instantaneous elastic deformation and instantaneous plastic deformation during the multi-level of load and unload in the cyclic process. In respect of specimen JC1099, the ratio of visco-elastic strain to visco-plastic strain varies from 3.15 to 6.58, and the ratio has decreasing tendency with stress increase as a whole; creep deformation tends to be a steady state at low stress level; soft rocks creep usually embodies accelerated creep properties at high stress level. With the damaging variable and the hardening function introduced, a nonlinear creep model of soft rocks is established, in which the decay creep is described by the nonlinear hardening function H of viscidity coefficient. The model can describe the accelerated creep of soft rocks since the nonlinear damaging evolvement variable D of deformation parameter of rocks is introduced. Three stages of soft rocks creep can be described with the uniform creep equation in the nonlinear creep model. With this nonlinear creep model applied to the creep experiments of the ore-rock of Jinchuan Mine III, the nonlinear creep model's curves are in good agreement with experimental data. 展开更多
关键词 rheological experiment nonlinear creep damaging HARDENING soft rocks
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