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无底物情况下来自Rhodococcus zopfii的腈水解酶中亲核进攻试剂CYS165的活性状态的探究(英文)
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作者 张慧珠 高旭丹 梅晔 《南京大学学报(自然科学版)》 北大核心 2025年第1期129-139,共11页
腈水解酶作为一类在工业应用中具有巨大价值的绿色生物催化剂,能够高效地催化腈基化合物转化为羧酸.尽管其应用广泛,但腈水解酶的具体催化机制仍然不明确.先前的研究揭示了腈水解酶活性中心的GLU-LYS-GLU-CYS四联体在催化中起到关键作用... 腈水解酶作为一类在工业应用中具有巨大价值的绿色生物催化剂,能够高效地催化腈基化合物转化为羧酸.尽管其应用广泛,但腈水解酶的具体催化机制仍然不明确.先前的研究揭示了腈水解酶活性中心的GLU-LYS-GLU-CYS四联体在催化中起到关键作用,其中CYS残基作为亲核试剂攻击腈基,其硫醇基团的电离是反应中的一个关键步骤,然而,CYS的去质子化过程尚未被清晰揭示.研究聚焦于来自Rhodococcus zopfii(RzNIT)的腈水解酶,并研究了底物尚未进入酶活性位点时CYS165的质子化状态.通过对CYS165去质子化可能路径的详细分析,确认了在无底物状态下RzNIT中的CYS165处于中性状态.这一发现为进一步研究RzNIT的催化机制奠定了基础. 展开更多
关键词 ONIOM(the Own n-Layered Integrated Molecular Orbital and Molecular Mechanics) 腈水解酶 能垒 催化机制
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Study on a Bowl-based Mechanism for Transplanting Potted Strawberry Seedlings
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作者 Yin Da-qing Yang Yu-chao +2 位作者 Zhou Mai-le Wei Ming-xu Wang Jin-wu 《Journal of Northeast Agricultural University(English Edition)》 CAS 2023年第1期56-68,共13页
To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to ac... To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism. 展开更多
关键词 potted strawberry seedlings potted seedling transplanting integrated planting mechanism protruding and pushing bowl type parameter optimization
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