Estimate of the Deterministic Neutron RBE for Radiation-induced Pseudo-Pelger Huët Cell Formation R.E.Goans1,2,C.J.Iddins1,R.E.Goans,Jr.3(1.Radiation Emergency Assistance Center/Training Site,Oak Ridge,TN;2.MJW C...Estimate of the Deterministic Neutron RBE for Radiation-induced Pseudo-Pelger Huët Cell Formation R.E.Goans1,2,C.J.Iddins1,R.E.Goans,Jr.3(1.Radiation Emergency Assistance Center/Training Site,Oak Ridge,TN;2.MJW Corporation,Amherst,NY;3.LMU Debusk School of Medicine,Harrogate,TN)Abstract:Using archival peripheral blood slides from radiation accident patients,we have recently described the pseudo-Pelger Huët anomaly(PPHA)in neutrophils as a new radiation-induced biomarker,useful for dosimetry not only immediately after a radiation incident but also potentially helpful as a tool in retrospective dosimetry.展开更多
Heterogeneous structure exhibits superiority in improving mechanical properties,whereas their effects on fatigue damage properties have rarely been studied.In this work,we employed a high-throughput gradient heat trea...Heterogeneous structure exhibits superiority in improving mechanical properties,whereas their effects on fatigue damage properties have rarely been studied.In this work,we employed a high-throughput gradient heat treatment method(757−857℃)to rapidly acquire the solution microstructure of the Ti-6554 alloy with different recrystallization degrees(0%,40%and 100%),followed by the same aging treatment.The results showed that theβ-hetero structure exhibited a yield strength(σ_(YS))of 1403 MPa,an increase of 6.7%,and a remarkable improvement in uniform elongation(UE)of 109.7%,reaching 6.5%,compared to the homogeneous structure.Interestingly,introducing a heterogeneous structure not only overcame the traditional trade-off between strength and ductility but also enhanced fatigue crack propagation(FCP)performance.During FCP process,β-hetero structure,through hetero-deformation induced(HDI)strengthening effects,promoted the accumulation of geometric necessary dislocations(GNDs)within coarseα_(S) phase,enabling faster attainment of the critical shear stress of twinning and increasing twinning density.This facilitated stress relief,improved plastic deformation in the crack tip zone,and increased the critical fast fracture threshold from 30.4 to 36.0 MPa·m^(1/2)showing an enlarged steady state propagation region.This study provides valuable insights on tailoring fatigue damage tolerance through heterogeneous structure for titanium alloys.展开更多
提出了一种基于频繁子树模式的GML文档结构聚类算法GCFS(GML Clustering based on Frequent Subtree patterns),与其他相关算法不同,该算法首先挖掘GML文档集合中的最大与闭合频繁Induced子树,并将其作为聚类特征,根据频繁子树的大小赋...提出了一种基于频繁子树模式的GML文档结构聚类算法GCFS(GML Clustering based on Frequent Subtree patterns),与其他相关算法不同,该算法首先挖掘GML文档集合中的最大与闭合频繁Induced子树,并将其作为聚类特征,根据频繁子树的大小赋予不同的权值,采用余弦函数定义相似度,利用K-Means算法对聚类特征进行聚类。实验结果表明算法GCFS是有效的,具有较高的聚类效率,性能优于其他同类算法。展开更多
Wind erosion is a major cause of land desertification and sandstorm formation in arid and semi-arid areas.The objective of this study was to evaluate the potential of soybeans crude extract induced calcium carbonate p...Wind erosion is a major cause of land desertification and sandstorm formation in arid and semi-arid areas.The objective of this study was to evaluate the potential of soybeans crude extract induced calcium carbonate precipitation(SICP)on reducing wind erosion risk of sandy soil.Field tests were carried out in Ulan Buh Desert,Ningxia Hui Autonomous Region,China.Results showed that the SICP method could significantly enhance the surface strength and wind erosion resistance of the topsoil.The optimal cementation solution(urea-CaCl2)concentration and spraying volume,according to experiments conducted on sandy land,were 0.2 mol/L and 4 L/m^2,respectively.Under this condition,the CaCO3 content was approximately 0.45%,the surface strength of sandy soil could reach 306.2 kPa,and the depth of wind erosion was approximately zero,after 30 d completion of SICP treatment.Soil surface strength declined with the increase of time,and long-term sand fixation effects of SICP treatment varied depending on topography.Whereas wind erosion in the top area of the windward slope was remarkable,sandy soils on the bottom area of the windward slope still maintained a relatively high level of surface strength and a low degree of wind erosion 12 month after SICP treatment.Scanning electron microscopy(SEM)tests with energy dispersive X-ray(EDX)confirmed the precipitation of CaCO3 and its bridge effect.These findings suggested that the SICP method is a promising candidate to protect sandy soil from wind erosion in desert areas.展开更多
With the help of similar material simulation test,time series system for induced caving of roof in continuous mining under complex backfill in ore body No.92 of Tongkeng Tin Mine was studied. According to the similari...With the help of similar material simulation test,time series system for induced caving of roof in continuous mining under complex backfill in ore body No.92 of Tongkeng Tin Mine was studied. According to the similarity theory,a two-dimensional similar simulation test-bed was constructed. The stress and displacement that change along with the advance of mining were acquired and analyzed automatically by data system. The processes of continuous mining of ore-block in 5 intervals and artificial induced caving of roof were simulated. The results of the test show that ore body remained as safety roof in thickness of 15 m guarantees the safe advance of stoping work face. Caving of safety roof puts in practice at the first two mining intervals when the third interval of continuous mining is finished,and one interval as the safety distance should be kept all the time between stopping and caving. While mining in the last interval,pre-slotting should be implemented first of all,and the roof of the last two mining intervals is caved simultaneously. Only this kind of time series system can be an efficient and safe way for induced caving of roof in continuous mining.展开更多
In order to perfectly reflect the dynamic corrosion of reinforced concrete (RC) cover in practical engineering,an analytic model of non-uniform corrosion induced cracking was presented based on the elastic-plastic fra...In order to perfectly reflect the dynamic corrosion of reinforced concrete (RC) cover in practical engineering,an analytic model of non-uniform corrosion induced cracking was presented based on the elastic-plastic fracture mechanics theory.Comparisons with the published experimental data show that the predictions given by the present model are in good agreement with the results both for natural exposed experiments and short-time indoor tests (the best difference is about 2.7%).Also it obviously provides much better precision than those models under the assumption of uniform corrosion (the maximal improved precision is about 48%).Therefore,it is pointed out that the so-called uniform corrosion models to describe the cover cracking of RC should be adopted cautiously.Finally,the influences of thickness of local rusty layer around the reinforcing steel bar on the critical corrosion-induced crack indexes were investigated.It is found that the thickness of local rusty layer has great effect on the critical mass loss of reinforcing steel,threshold expansion pressure,and time to cover cracking.For local rusty layer thickness with a size of a=0.5 mm,the time to cover cracking will increase by about one times when a/b (a,semi-minor axis;b,semi-major axis) changes from 0.1 to 1 mm.展开更多
Background:In our previous study,a strain EBS03 with good biocontrol potential was screened out of 48 strains of cotton endophyte Bacillus subtilis by evaluating the controlling effect against cotton Verticillium wilt...Background:In our previous study,a strain EBS03 with good biocontrol potential was screened out of 48 strains of cotton endophyte Bacillus subtilis by evaluating the controlling effect against cotton Verticillium wilt.However,its mechanism for controlling Verticillium wilt remains unclear.The objective of this study was to further clarify its con-trolling effect and mechanism against cotton Verticillium wilt.Results:The results of confrontation culture test and double buckle culture test showed that the inhibitory effects of EBS03 volatile and nonvolatile metabolite on mycelium growth of Verticillium dahliae were 70.03%and 59.00%,respectively;the inhibitory effects of sporulation and microsclerotia germination were 47.16%and 70.06%,respec-tively.In the greenhouse test,the EBS03 fermentation broth root irrigation had the highest controlling effect at 87.11%on cotton Verticillium wilt,and significantly promoted the growth of cotton seedlings.In the field experi-ment,the controlling effect of EBS03 fermentation broth to cotton Verticillium wilt was 42.54%at 60 days after cotton sowing,and the boll number per plant and boll weight in EBS03 fermentation broth seed soaking,root irrigation,and spraying treatments significantly increased by 19.48%and 7.42%,30.90%and 2.62%,15.99%and 9.20%,respec-tively.Furthermore,EBS03 improved the resistance of cotton leaves against the infection of V.dahliae,and induced the outbreak of reactive oxygen species and accumulation of callose.In addition,the results of real time fluorescent quantitative polymerase chain reaction(RT-qPCR)detection showed that EBS03 significantly induced upregulation expression level of defense-related genes PAL,POD,PPO,and PR10 in cotton leaves,enhanced cotton plant resistance to V.dahliae,and inhibited colonization level of this fungal pathogen in cotton.Conclusion:Bacillus subtilis EBS03 has a good biological defense capability,which can inhibit the growth and coloni-zation level of V.dahliae,and activate the resistance of cotton to Verticillium wilt,thus increase cotton yield.展开更多
A systematic study of air gap distance effects on the structure and properties of poly(vinyl butyral)hollow fiber membrane via thermally induced phase separation(TIPS)has been carried out.The results show that the hol...A systematic study of air gap distance effects on the structure and properties of poly(vinyl butyral)hollow fiber membrane via thermally induced phase separation(TIPS)has been carried out.The results show that the hollow fiber membrane prepared at air gap zero has no skin layer; the pore size near the outer surface is larger than that near the inner surface; and the special pore channel-like structure near the outer surface is formed,which is quite different with the typical sponge-like structure caused by TIPS and the finger-like structure caused by non-solvent induced phase separation(NIPS),because of the synergistic action of non-solvent induced phase separation at air gap zero.The pore size gradually decreases from outer surface layer to the intermediate layer,but increases gradually from intermediate layer to the inner surface layer.With the increase of air gap distance,the pore size near the outer surface gets smaller and a dense skin layer is formed,and the pore size gradually increases from the outer surface layer to the inner surface layer.Water permeability of the hollow fiber membrane decreases with air gap distance,the water permeability decreases sharply from 45.50×10-7 to 4.52×10-7 m3/(m2·s·kPa)as air gap increases from 0 to 10 mm at take-up speed of 0.236 m/s,further decreases from 4.52×10-7 to 1.00×10-8 m3/(m2·s·kPa)as the air gap increases from 10 to 40 mm.Both the breaking strength and the elongation increase with the increase of air gap distance.The breaking strength increases from 2.25 MPa to 4.19 MPa and the elongation increases from 33.9% to 132.6% as air gap increases from 0 mm to 40 mm at take-up speed 0.236 m/s.展开更多
There are many parameters influencing mining induced surface subsidence. These parameters usually interact with one another and some of them have the characteristic of fuzziness. Current approaches to predicting the s...There are many parameters influencing mining induced surface subsidence. These parameters usually interact with one another and some of them have the characteristic of fuzziness. Current approaches to predicting the subsidence cannot take into account of such interactions and fuzziness. In order to overcome this disadvantage, many mining induced surface subsidence cases were accumulated, and an artificial neuro fuzzy inference system(ANFIS) was used to set up 4 ANFIS models to predict the rise angle, dip angle, center angle and the maximum subsidence, respectively. The fitting and generalization prediction capabilities of the models were tested. The test results show that the models have very good fitting and generalization prediction capabilities and the approach can be applied to predict the mining induced surface subsidence.展开更多
To investigate the relationship between nonlinear parameters and spontaneous combustion tendency of sulfide ores, nine different sulfide ore samples were taken from a pyrite mine in China, and induced spontaneous comb...To investigate the relationship between nonlinear parameters and spontaneous combustion tendency of sulfide ores, nine different sulfide ore samples were taken from a pyrite mine in China, and induced spontaneous combustion experiment was carried out in the laboratory. Different stages of the induced spontaneous combustion process were studied by integrating wavelet technology and nonlinear dynamics theory. The results show that ignition points of all the ore samples are above 330 ℃, indicating that sulfide ores of the pyrite mine are difficult to combust spontaneously under normal mining conditions. Spontaneous combustion process includes three stages: incubation stage, development stage and approaching stage. The average temperature rising rate of the three stages are 1.0 ~C/min, 2.0 ~C/min and 4.2 ~C/min, respectively. During the spontaneous combustion process, mean values of approximate entropy and correlation dimension increase at first, and then decrease in the following stage. The mean value of the maximum Lyapunov exponent increases with the passage of reaction time. In a whole, correlation among the three nonlinear parameters firstly weakens, then enhances, and the best correlation period is at approaching stage. As ignition point increases, the maximum Lyapunov exponent of approaching stage decreases. Therefore, combustible tendency of sulfide ores could be qualitatively evaluated based on the maximum Lyapunov exponent of this stage.展开更多
Solar arrays are important and indispensable parts of spacecraft and provide energy support for spacecraft to operate in orbit and complete on-orbit missions.When a spacecraft is in orbit,because the solar array is ex...Solar arrays are important and indispensable parts of spacecraft and provide energy support for spacecraft to operate in orbit and complete on-orbit missions.When a spacecraft is in orbit,because the solar array is exposed to the harsh space environment,with increasing working time,the performance of its internal electronic components gradually degrade until abnormal damage occurs.This damage makes solar array power generation unable to fully meet the energy demand of a spacecraft.Therefore,timely and accurate detection of solar array anomalies is of great significance for the on-orbit operation and maintenance management of spacecraft.In this paper,we propose an anomaly detection method for spacecraft solar arrays based on the integrated least squares support vector machine(ILS-SVM)model:it selects correlated telemetry data from spacecraft solar arrays to form a training set and extracts n groups of training subsets from this set,then gets n corresponding least squares support vector machine(LS-SVM)submodels by training on these training subsets,respectively;after that,the ILS-SVM model is obtained by integrating these submodels through a weighting operation to increase the prediction accuracy and so on;finally,based on the obtained ILS-SVM model,a parameterfree and unsupervised anomaly determination method is proposed to detect the health status of solar arrays.We use the telemetry data set from a satellite in orbit to carry out experimental verification and find that the proposed method can diagnose solar array anomalies in time and can capture the signs before a solar array anomaly occurs,which reflects the applicability of the method.展开更多
The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stre...The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity.展开更多
All underwater drilling and blasting operations generate seismic waves.However,due to a lack of suitable vibration sensing instruments,most studies on the propagation of seismic waves have been limited to shorelines n...All underwater drilling and blasting operations generate seismic waves.However,due to a lack of suitable vibration sensing instruments,most studies on the propagation of seismic waves have been limited to shorelines near construction areas or wharfs,whereas comparatively few studies have beerconducted on the larger seafloor itself.To address this gap,a seafloor vibration sensor system was developed and applied in this study that consists of an autonomous acquisition storage terminal,soft-ware platform,and hole-plugging device that was designed to record the blasting vibration intensities received through submarine rocks at a given measurement point.Additionally,dimensional analyses were used to derive a predictive equation for the strength of blast vibrations that considered the in fluence of the water depth.By combining reliable vibration data obtained using the sensor system in submarine rock and the developed predictive equation,it was determined that the water depth was ar important factor influencing the measured vibration strength.The results using the newly derivedequation were compared to those determined using the Sadowski equation,which is commonly used on land,and it was found that predictions using the derived equation were closer to the experimental values with an average error of less than 10%,representing a significant improvement.Based on these results the developed sensor system and preliminary theoretical basis was deemed suitable for studying the propagation behavior of submarine seismic waves generated by underwater drilling and blasting operations.展开更多
文摘Estimate of the Deterministic Neutron RBE for Radiation-induced Pseudo-Pelger Huët Cell Formation R.E.Goans1,2,C.J.Iddins1,R.E.Goans,Jr.3(1.Radiation Emergency Assistance Center/Training Site,Oak Ridge,TN;2.MJW Corporation,Amherst,NY;3.LMU Debusk School of Medicine,Harrogate,TN)Abstract:Using archival peripheral blood slides from radiation accident patients,we have recently described the pseudo-Pelger Huët anomaly(PPHA)in neutrophils as a new radiation-induced biomarker,useful for dosimetry not only immediately after a radiation incident but also potentially helpful as a tool in retrospective dosimetry.
基金Project(2021YFB3700801)supported by the National Key Research and Development Program of ChinaProject(2023JJ30683)supported by the Natural Science Foundation of Hunan Province,ChinaProject supported by the State Key Laboratory of Powder Metallurgy(Central South University),China。
文摘Heterogeneous structure exhibits superiority in improving mechanical properties,whereas their effects on fatigue damage properties have rarely been studied.In this work,we employed a high-throughput gradient heat treatment method(757−857℃)to rapidly acquire the solution microstructure of the Ti-6554 alloy with different recrystallization degrees(0%,40%and 100%),followed by the same aging treatment.The results showed that theβ-hetero structure exhibited a yield strength(σ_(YS))of 1403 MPa,an increase of 6.7%,and a remarkable improvement in uniform elongation(UE)of 109.7%,reaching 6.5%,compared to the homogeneous structure.Interestingly,introducing a heterogeneous structure not only overcame the traditional trade-off between strength and ductility but also enhanced fatigue crack propagation(FCP)performance.During FCP process,β-hetero structure,through hetero-deformation induced(HDI)strengthening effects,promoted the accumulation of geometric necessary dislocations(GNDs)within coarseα_(S) phase,enabling faster attainment of the critical shear stress of twinning and increasing twinning density.This facilitated stress relief,improved plastic deformation in the crack tip zone,and increased the critical fast fracture threshold from 30.4 to 36.0 MPa·m^(1/2)showing an enlarged steady state propagation region.This study provides valuable insights on tailoring fatigue damage tolerance through heterogeneous structure for titanium alloys.
文摘提出了一种基于频繁子树模式的GML文档结构聚类算法GCFS(GML Clustering based on Frequent Subtree patterns),与其他相关算法不同,该算法首先挖掘GML文档集合中的最大与闭合频繁Induced子树,并将其作为聚类特征,根据频繁子树的大小赋予不同的权值,采用余弦函数定义相似度,利用K-Means算法对聚类特征进行聚类。实验结果表明算法GCFS是有效的,具有较高的聚类效率,性能优于其他同类算法。
基金Projects(51978244,51979088,51608169)supported by the National Natural Science Foundation of China。
文摘Wind erosion is a major cause of land desertification and sandstorm formation in arid and semi-arid areas.The objective of this study was to evaluate the potential of soybeans crude extract induced calcium carbonate precipitation(SICP)on reducing wind erosion risk of sandy soil.Field tests were carried out in Ulan Buh Desert,Ningxia Hui Autonomous Region,China.Results showed that the SICP method could significantly enhance the surface strength and wind erosion resistance of the topsoil.The optimal cementation solution(urea-CaCl2)concentration and spraying volume,according to experiments conducted on sandy land,were 0.2 mol/L and 4 L/m^2,respectively.Under this condition,the CaCO3 content was approximately 0.45%,the surface strength of sandy soil could reach 306.2 kPa,and the depth of wind erosion was approximately zero,after 30 d completion of SICP treatment.Soil surface strength declined with the increase of time,and long-term sand fixation effects of SICP treatment varied depending on topography.Whereas wind erosion in the top area of the windward slope was remarkable,sandy soils on the bottom area of the windward slope still maintained a relatively high level of surface strength and a low degree of wind erosion 12 month after SICP treatment.Scanning electron microscopy(SEM)tests with energy dispersive X-ray(EDX)confirmed the precipitation of CaCO3 and its bridge effect.These findings suggested that the SICP method is a promising candidate to protect sandy soil from wind erosion in desert areas.
基金Project(50490274) supported by the National Natural Science Foundation of ChinaProject(20050533035) supported by the Specialized Research Fund for the Doctoral Program of Higher Education of ChinaProject(1343-77236) supported by the Doctor Degree Paper Innovation Engineering of Central South University, China
文摘With the help of similar material simulation test,time series system for induced caving of roof in continuous mining under complex backfill in ore body No.92 of Tongkeng Tin Mine was studied. According to the similarity theory,a two-dimensional similar simulation test-bed was constructed. The stress and displacement that change along with the advance of mining were acquired and analyzed automatically by data system. The processes of continuous mining of ore-block in 5 intervals and artificial induced caving of roof were simulated. The results of the test show that ore body remained as safety roof in thickness of 15 m guarantees the safe advance of stoping work face. Caving of safety roof puts in practice at the first two mining intervals when the third interval of continuous mining is finished,and one interval as the safety distance should be kept all the time between stopping and caving. While mining in the last interval,pre-slotting should be implemented first of all,and the roof of the last two mining intervals is caved simultaneously. Only this kind of time series system can be an efficient and safe way for induced caving of roof in continuous mining.
基金Project(50925829) supported by the National Science Fund for Distinguished Young Scholars of ChinaProject(50908148) supported by the National Natural Science Foundation of ChinaProjects(2009-K4-23,2010-11-33) supported by the Research of Ministry of Housing and Urban Rural Development of China
文摘In order to perfectly reflect the dynamic corrosion of reinforced concrete (RC) cover in practical engineering,an analytic model of non-uniform corrosion induced cracking was presented based on the elastic-plastic fracture mechanics theory.Comparisons with the published experimental data show that the predictions given by the present model are in good agreement with the results both for natural exposed experiments and short-time indoor tests (the best difference is about 2.7%).Also it obviously provides much better precision than those models under the assumption of uniform corrosion (the maximal improved precision is about 48%).Therefore,it is pointed out that the so-called uniform corrosion models to describe the cover cracking of RC should be adopted cautiously.Finally,the influences of thickness of local rusty layer around the reinforcing steel bar on the critical corrosion-induced crack indexes were investigated.It is found that the thickness of local rusty layer has great effect on the critical mass loss of reinforcing steel,threshold expansion pressure,and time to cover cracking.For local rusty layer thickness with a size of a=0.5 mm,the time to cover cracking will increase by about one times when a/b (a,semi-minor axis;b,semi-major axis) changes from 0.1 to 1 mm.
基金This work was supported by the National Natural Science Foundation of China(No.32201752)the Central Public-interest Scientific Institution Basal Research Fund(No.1610162022018),Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences.
文摘Background:In our previous study,a strain EBS03 with good biocontrol potential was screened out of 48 strains of cotton endophyte Bacillus subtilis by evaluating the controlling effect against cotton Verticillium wilt.However,its mechanism for controlling Verticillium wilt remains unclear.The objective of this study was to further clarify its con-trolling effect and mechanism against cotton Verticillium wilt.Results:The results of confrontation culture test and double buckle culture test showed that the inhibitory effects of EBS03 volatile and nonvolatile metabolite on mycelium growth of Verticillium dahliae were 70.03%and 59.00%,respectively;the inhibitory effects of sporulation and microsclerotia germination were 47.16%and 70.06%,respec-tively.In the greenhouse test,the EBS03 fermentation broth root irrigation had the highest controlling effect at 87.11%on cotton Verticillium wilt,and significantly promoted the growth of cotton seedlings.In the field experi-ment,the controlling effect of EBS03 fermentation broth to cotton Verticillium wilt was 42.54%at 60 days after cotton sowing,and the boll number per plant and boll weight in EBS03 fermentation broth seed soaking,root irrigation,and spraying treatments significantly increased by 19.48%and 7.42%,30.90%and 2.62%,15.99%and 9.20%,respec-tively.Furthermore,EBS03 improved the resistance of cotton leaves against the infection of V.dahliae,and induced the outbreak of reactive oxygen species and accumulation of callose.In addition,the results of real time fluorescent quantitative polymerase chain reaction(RT-qPCR)detection showed that EBS03 significantly induced upregulation expression level of defense-related genes PAL,POD,PPO,and PR10 in cotton leaves,enhanced cotton plant resistance to V.dahliae,and inhibited colonization level of this fungal pathogen in cotton.Conclusion:Bacillus subtilis EBS03 has a good biological defense capability,which can inhibit the growth and coloni-zation level of V.dahliae,and activate the resistance of cotton to Verticillium wilt,thus increase cotton yield.
基金Project(21176264)supported by the National Natural Science Foundation of ChinaProject(11JJ2010)supported by the Natural Science Foundation of Hunan Province,China
文摘A systematic study of air gap distance effects on the structure and properties of poly(vinyl butyral)hollow fiber membrane via thermally induced phase separation(TIPS)has been carried out.The results show that the hollow fiber membrane prepared at air gap zero has no skin layer; the pore size near the outer surface is larger than that near the inner surface; and the special pore channel-like structure near the outer surface is formed,which is quite different with the typical sponge-like structure caused by TIPS and the finger-like structure caused by non-solvent induced phase separation(NIPS),because of the synergistic action of non-solvent induced phase separation at air gap zero.The pore size gradually decreases from outer surface layer to the intermediate layer,but increases gradually from intermediate layer to the inner surface layer.With the increase of air gap distance,the pore size near the outer surface gets smaller and a dense skin layer is formed,and the pore size gradually increases from the outer surface layer to the inner surface layer.Water permeability of the hollow fiber membrane decreases with air gap distance,the water permeability decreases sharply from 45.50×10-7 to 4.52×10-7 m3/(m2·s·kPa)as air gap increases from 0 to 10 mm at take-up speed of 0.236 m/s,further decreases from 4.52×10-7 to 1.00×10-8 m3/(m2·s·kPa)as the air gap increases from 10 to 40 mm.Both the breaking strength and the elongation increase with the increase of air gap distance.The breaking strength increases from 2.25 MPa to 4.19 MPa and the elongation increases from 33.9% to 132.6% as air gap increases from 0 mm to 40 mm at take-up speed 0.236 m/s.
基金Project(50274043) supported by the National Natural Science Foundation of China project (01JJY1004) supported bythe Natural Science Foundation of Hunan Province project (01A015) supported by the Natural Science Foundation of Hunan ProvincialEducation Committee
文摘There are many parameters influencing mining induced surface subsidence. These parameters usually interact with one another and some of them have the characteristic of fuzziness. Current approaches to predicting the subsidence cannot take into account of such interactions and fuzziness. In order to overcome this disadvantage, many mining induced surface subsidence cases were accumulated, and an artificial neuro fuzzy inference system(ANFIS) was used to set up 4 ANFIS models to predict the rise angle, dip angle, center angle and the maximum subsidence, respectively. The fitting and generalization prediction capabilities of the models were tested. The test results show that the models have very good fitting and generalization prediction capabilities and the approach can be applied to predict the mining induced surface subsidence.
基金Projects(51304238,51534008)supported by the National Natural Science Foundation of ChinaProject(2015CX005)supported by Innovation Driven Plan of Central South University,China
文摘To investigate the relationship between nonlinear parameters and spontaneous combustion tendency of sulfide ores, nine different sulfide ore samples were taken from a pyrite mine in China, and induced spontaneous combustion experiment was carried out in the laboratory. Different stages of the induced spontaneous combustion process were studied by integrating wavelet technology and nonlinear dynamics theory. The results show that ignition points of all the ore samples are above 330 ℃, indicating that sulfide ores of the pyrite mine are difficult to combust spontaneously under normal mining conditions. Spontaneous combustion process includes three stages: incubation stage, development stage and approaching stage. The average temperature rising rate of the three stages are 1.0 ~C/min, 2.0 ~C/min and 4.2 ~C/min, respectively. During the spontaneous combustion process, mean values of approximate entropy and correlation dimension increase at first, and then decrease in the following stage. The mean value of the maximum Lyapunov exponent increases with the passage of reaction time. In a whole, correlation among the three nonlinear parameters firstly weakens, then enhances, and the best correlation period is at approaching stage. As ignition point increases, the maximum Lyapunov exponent of approaching stage decreases. Therefore, combustible tendency of sulfide ores could be qualitatively evaluated based on the maximum Lyapunov exponent of this stage.
基金supported by the National Natural Science Foundation of China(7190121061973310).
文摘Solar arrays are important and indispensable parts of spacecraft and provide energy support for spacecraft to operate in orbit and complete on-orbit missions.When a spacecraft is in orbit,because the solar array is exposed to the harsh space environment,with increasing working time,the performance of its internal electronic components gradually degrade until abnormal damage occurs.This damage makes solar array power generation unable to fully meet the energy demand of a spacecraft.Therefore,timely and accurate detection of solar array anomalies is of great significance for the on-orbit operation and maintenance management of spacecraft.In this paper,we propose an anomaly detection method for spacecraft solar arrays based on the integrated least squares support vector machine(ILS-SVM)model:it selects correlated telemetry data from spacecraft solar arrays to form a training set and extracts n groups of training subsets from this set,then gets n corresponding least squares support vector machine(LS-SVM)submodels by training on these training subsets,respectively;after that,the ILS-SVM model is obtained by integrating these submodels through a weighting operation to increase the prediction accuracy and so on;finally,based on the obtained ILS-SVM model,a parameterfree and unsupervised anomaly determination method is proposed to detect the health status of solar arrays.We use the telemetry data set from a satellite in orbit to carry out experimental verification and find that the proposed method can diagnose solar array anomalies in time and can capture the signs before a solar array anomaly occurs,which reflects the applicability of the method.
文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity.
文摘All underwater drilling and blasting operations generate seismic waves.However,due to a lack of suitable vibration sensing instruments,most studies on the propagation of seismic waves have been limited to shorelines near construction areas or wharfs,whereas comparatively few studies have beerconducted on the larger seafloor itself.To address this gap,a seafloor vibration sensor system was developed and applied in this study that consists of an autonomous acquisition storage terminal,soft-ware platform,and hole-plugging device that was designed to record the blasting vibration intensities received through submarine rocks at a given measurement point.Additionally,dimensional analyses were used to derive a predictive equation for the strength of blast vibrations that considered the in fluence of the water depth.By combining reliable vibration data obtained using the sensor system in submarine rock and the developed predictive equation,it was determined that the water depth was ar important factor influencing the measured vibration strength.The results using the newly derivedequation were compared to those determined using the Sadowski equation,which is commonly used on land,and it was found that predictions using the derived equation were closer to the experimental values with an average error of less than 10%,representing a significant improvement.Based on these results the developed sensor system and preliminary theoretical basis was deemed suitable for studying the propagation behavior of submarine seismic waves generated by underwater drilling and blasting operations.