The changes of carbamyl phosphate synthetase I(CPS 1)in diethylnitrosamine-(DEN)-inducedenzyme-altered liver cells were studied by means of immunohistochemical(PAP)and in situcDNA-mRNA hybridization methods.The experi...The changes of carbamyl phosphate synthetase I(CPS 1)in diethylnitrosamine-(DEN)-inducedenzyme-altered liver cells were studied by means of immunohistochemical(PAP)and in situcDNA-mRNA hybridization methods.The experimental rats were treated with DEN,2-acetylaminofluorene(2-AAF)and 2/3 hepatectomy according to Solt-Farber’s protocol andwere further promoted by oral daily administration of 0.05% phenobarbital in drinking water.The results showed that the average number of lesions showing abnormal expression of CPS1 was relatively constant over the course of the experiment(8 months),while the numberof normally expressing lesions gradually decreased.The former lesions were also largerin volume than the latter ones.We conclude that in DEN-initiated lesions the abnormallyexpressed CPS 1 lesions may grow continuously,thus leading to the formation of largernodules.We also suspect that some of these lesions have increased tendencies to developinto tumors.展开更多
HUMAN herpes simplex virus esophagitis （HSVE） was first reported in 1940 by Johnson. ^1HSVE usually occurs in immunocompromised patients,such as those with acquired immunodeficiency syndrome （AIDS）, 2-4 malignanc...HUMAN herpes simplex virus esophagitis （HSVE） was first reported in 1940 by Johnson. ^1HSVE usually occurs in immunocompromised patients,such as those with acquired immunodeficiency syndrome （AIDS）, 2-4 malignancies, cutaneous burns, connective tissue diseases, inflammatory bowel disease, those taking immuno-suppressive therapy, and those undergoing organ transplantation,5 etc. In the immunocompetent individuals, HSVE is rare, having been reported in 39 cases and mainly affecting young males^6,7 The aim of this study was to delineate the clinical experience in the diagnosis of HSVE using rapid in situ hybridization and assess the various detection methods.展开更多
Objective: To establish a method of non-isotope double in situ hybridization in order to detect the expression of two kinds of oncogenes at single cell level simultaneously, and confirm the hypothesis of 'model of...Objective: To establish a method of non-isotope double in situ hybridization in order to detect the expression of two kinds of oncogenes at single cell level simultaneously, and confirm the hypothesis of 'model of stepwise carcinogenesis'. Methods: The method of non-isotope double in situ hybridization was established with the digoxigenin (Dig) and biotin(Bio) labelled probes. The expression of two members of oncogenes of the myc and/or ras gene families (myc and N-ras, myc and K-ras, myc and H-ras, N-ras and K-ras , N-ras and H-ras, K-ras and H-ras) was further studied with the method in 10 cases of Chinese Hepatocellular Carcinomas (HCC). Results : Co-expression of two kinds of oncogenes was detected only in a few cases, reflected by coshowing two different hybridization signals ,i. e. , co-showing of Dig-myc and Bio-H-ras, Dig-myc and Bio-N-ras, Dig-myc and Bio-K-ras was observed in 2 cases, 2 cases and one case, respectively. The common characteristics were that positive cells of myc distributed diffusely, while positive cells of ras diatributed sporadically or locally among the positive cells of myc, and only a few cells exhibited co-showing of two oncogenes at single cell level. There were only 2 cases representing co-showing of two oncogenes in ras gene family (Dig-N-ras and bio-H-ras, Dig-N-ras and Bio-K-ras , respectively). The two kinds of positive cells of different ras gene represented mixed local and sporadical distribution, and co-showing of two signals was found in a few cells at single cell level. Conclusion: There are multiple oncogenes involving in tumorigenesis by their ordered activation. The activation of the ras gene family plays a role in promotion, while the activation of myc is an important event in late stage of tumorigenesis展开更多
Digoxigenin-labelled Hantaan specific cDNA probes were used in the present study for in situ hybridization detection of hemorrhagic fever with renal syndrome virus(HFRSV) persistent infection and viral RNA distributio...Digoxigenin-labelled Hantaan specific cDNA probes were used in the present study for in situ hybridization detection of hemorrhagic fever with renal syndrome virus(HFRSV) persistent infection and viral RNA distribution in naturally infected laboratory rat展开更多
线纹尖塘鳢(Oxyeleotris lineolata)具有典型性别生长二态性,雄鱼生长优势显著,doublesex and mab-3 related transcription factor(DMRT)家族是一个与性别决定相关的转录因子家族。基于线纹尖塘鳢性腺转录组数据,共获得2个dmrt基因的c...线纹尖塘鳢(Oxyeleotris lineolata)具有典型性别生长二态性,雄鱼生长优势显著,doublesex and mab-3 related transcription factor(DMRT)家族是一个与性别决定相关的转录因子家族。基于线纹尖塘鳢性腺转录组数据,共获得2个dmrt基因的cDNA序列,分别命名为Oxldmrt1和Oxldmrt3,并采用PCR技术扩增验证2个基因的cDNA序列。运用生物信息学方法分析2个基因序列结构特征,结果显示,Oxldmrt1和Oxldmrt3开放阅读框分别为903 bp和1363 bp,分别编码300个氨基酸和453个氨基酸;OxlDMRT1属于碱性蛋白,而OxlDMRT3属于酸性蛋白;2个基因均含有高度保守的DM结构域,OxlDMRT3还存在DMA结构域。氨基酸聚类分析显示,脊椎动物不同DMRT家族都是独立聚类,OxlDMRT1属于DMRT1家族,OxlDMRT3属于DMRT3家族,DMRT1家族最先聚类,再和DMRT3家族聚类。利用实时荧光定量PCR技术(real-time quantitative PCR,RT-qPCR)分析2个dmrt基因在雌鱼和雄鱼8个组织的表达水平,结果显示,2个基因在精巢中的表达量均显著高于其他组织(P<0.01),Oxldmrt3在脑中也有少量表达;利用RT-qPCR分析2个dmrt基因在早期不同发育时期的表达谱,显示2个基因在受精卵中的表达量均最高,Oxldmrt1在眼囊期的表达量最低,而Oxldmrt3在出膜7 d时的表达量最低。利用荧光原位杂交(fluorescencein situhybridization,FISH)对2个基因在精巢中的表达进行定位,显示2个基因在精巢中表达部位一致,均在精原细胞中有较强的表达信号。综上所述,Oxldmrt1和Oxldmrt3均在线纹尖塘鳢性腺胚胎发育阶段和精巢发育过程中起调节作用,而Oxldmrt1还可能参与胚胎后期性别决定和性别分化调控过程,Oxldmrt3还可能参与神经系统发育。本研究为线纹尖塘鳢性别决定与性别分化相关的分子机制研究提供了参考。展开更多
自然流产病因复杂,包括胚胎因素、母体因素、环境因素和免疫功能异常等,约50%~60%的流产与胚胎染色体异常有关。报告1例因2次自然流产行夫妇外周血染色体核型分析和荧光原位杂交(fluorescence in situ hybridization,FISH)检测的情况。...自然流产病因复杂,包括胚胎因素、母体因素、环境因素和免疫功能异常等,约50%~60%的流产与胚胎染色体异常有关。报告1例因2次自然流产行夫妇外周血染色体核型分析和荧光原位杂交(fluorescence in situ hybridization,FISH)检测的情况。妻子染色体核型未见明显异常,丈夫染色体核型46,XY,t(1;11)(p36.2;q24)。丈夫携带的t(1;11)平衡易位可能是导致该家庭反复流产的原因。该案例验证了FISH检测对于可疑亚端粒结构重排的应用价值。展开更多
文摘The changes of carbamyl phosphate synthetase I(CPS 1)in diethylnitrosamine-(DEN)-inducedenzyme-altered liver cells were studied by means of immunohistochemical(PAP)and in situcDNA-mRNA hybridization methods.The experimental rats were treated with DEN,2-acetylaminofluorene(2-AAF)and 2/3 hepatectomy according to Solt-Farber’s protocol andwere further promoted by oral daily administration of 0.05% phenobarbital in drinking water.The results showed that the average number of lesions showing abnormal expression of CPS1 was relatively constant over the course of the experiment(8 months),while the numberof normally expressing lesions gradually decreased.The former lesions were also largerin volume than the latter ones.We conclude that in DEN-initiated lesions the abnormallyexpressed CPS 1 lesions may grow continuously,thus leading to the formation of largernodules.We also suspect that some of these lesions have increased tendencies to developinto tumors.
文摘HUMAN herpes simplex virus esophagitis （HSVE） was first reported in 1940 by Johnson. ^1HSVE usually occurs in immunocompromised patients,such as those with acquired immunodeficiency syndrome （AIDS）, 2-4 malignancies, cutaneous burns, connective tissue diseases, inflammatory bowel disease, those taking immuno-suppressive therapy, and those undergoing organ transplantation,5 etc. In the immunocompetent individuals, HSVE is rare, having been reported in 39 cases and mainly affecting young males^6,7 The aim of this study was to delineate the clinical experience in the diagnosis of HSVE using rapid in situ hybridization and assess the various detection methods.
文摘Objective: To establish a method of non-isotope double in situ hybridization in order to detect the expression of two kinds of oncogenes at single cell level simultaneously, and confirm the hypothesis of 'model of stepwise carcinogenesis'. Methods: The method of non-isotope double in situ hybridization was established with the digoxigenin (Dig) and biotin(Bio) labelled probes. The expression of two members of oncogenes of the myc and/or ras gene families (myc and N-ras, myc and K-ras, myc and H-ras, N-ras and K-ras , N-ras and H-ras, K-ras and H-ras) was further studied with the method in 10 cases of Chinese Hepatocellular Carcinomas (HCC). Results : Co-expression of two kinds of oncogenes was detected only in a few cases, reflected by coshowing two different hybridization signals ,i. e. , co-showing of Dig-myc and Bio-H-ras, Dig-myc and Bio-N-ras, Dig-myc and Bio-K-ras was observed in 2 cases, 2 cases and one case, respectively. The common characteristics were that positive cells of myc distributed diffusely, while positive cells of ras diatributed sporadically or locally among the positive cells of myc, and only a few cells exhibited co-showing of two oncogenes at single cell level. There were only 2 cases representing co-showing of two oncogenes in ras gene family (Dig-N-ras and bio-H-ras, Dig-N-ras and Bio-K-ras , respectively). The two kinds of positive cells of different ras gene represented mixed local and sporadical distribution, and co-showing of two signals was found in a few cells at single cell level. Conclusion: There are multiple oncogenes involving in tumorigenesis by their ordered activation. The activation of the ras gene family plays a role in promotion, while the activation of myc is an important event in late stage of tumorigenesis
文摘Digoxigenin-labelled Hantaan specific cDNA probes were used in the present study for in situ hybridization detection of hemorrhagic fever with renal syndrome virus(HFRSV) persistent infection and viral RNA distribution in naturally infected laboratory rat
文摘自然流产病因复杂,包括胚胎因素、母体因素、环境因素和免疫功能异常等,约50%~60%的流产与胚胎染色体异常有关。报告1例因2次自然流产行夫妇外周血染色体核型分析和荧光原位杂交(fluorescence in situ hybridization,FISH)检测的情况。妻子染色体核型未见明显异常,丈夫染色体核型46,XY,t(1;11)(p36.2;q24)。丈夫携带的t(1;11)平衡易位可能是导致该家庭反复流产的原因。该案例验证了FISH检测对于可疑亚端粒结构重排的应用价值。
