The changes of carbamyl phosphate synthetase I(CPS 1)in diethylnitrosamine-(DEN)-inducedenzyme-altered liver cells were studied by means of immunohistochemical(PAP)and in situcDNA-mRNA hybridization methods.The experi...The changes of carbamyl phosphate synthetase I(CPS 1)in diethylnitrosamine-(DEN)-inducedenzyme-altered liver cells were studied by means of immunohistochemical(PAP)and in situcDNA-mRNA hybridization methods.The experimental rats were treated with DEN,2-acetylaminofluorene(2-AAF)and 2/3 hepatectomy according to Solt-Farber’s protocol andwere further promoted by oral daily administration of 0.05% phenobarbital in drinking water.The results showed that the average number of lesions showing abnormal expression of CPS1 was relatively constant over the course of the experiment(8 months),while the numberof normally expressing lesions gradually decreased.The former lesions were also largerin volume than the latter ones.We conclude that in DEN-initiated lesions the abnormallyexpressed CPS 1 lesions may grow continuously,thus leading to the formation of largernodules.We also suspect that some of these lesions have increased tendencies to developinto tumors.展开更多
One hundred and sixty-eight specimens of intestinal metaplasia(IM)with variousaccompanying lesions in gastric mucosa were studied with mucohistochemical and ABCimmunohistochemical staining,The quantitative analysis of...One hundred and sixty-eight specimens of intestinal metaplasia(IM)with variousaccompanying lesions in gastric mucosa were studied with mucohistochemical and ABCimmunohistochemical staining,The quantitative analysis of cell DNA was done withflowcytometry for 36 specimens.The results indicated that the incidence of type Ⅱb IM wassignificantly higher in the groups of dysplasia(34.6％)and mucosa adjacent to gastric cancer(GC)(51.7％)than in the chronic atrophic gastritis(CAG)group(16.0％)(P【0.01).The expres-sion rate of monoclonal antibody MG7 related antigen(MG7-Ag)in type Ⅱb IM(473％)wasalso significantly higher than those in type la(29.7％),Ib(26.1％)and Ⅱa IM(28.3％)(P【0.05).Expression rate of MG7-Ag,DNA aneuploid rate and percentage of S phase cell werestatistically higher in the type Ⅱb IM with dysphsia(62.5％,62.5％ and 143±32)than in typeⅡb 1M without dysplasia(47.3％,12.5％and12.7±2.9)(P【0.05 and P【0.01).These findingssuppor the supposed progressive process:CAG→type Ⅱb IM→dysplasia→GC,andtype Ⅱb IM with dysplasia is closely associated with GC.展开更多
Background The well-known ‘pyrotherapy’ of Julius Wagner-Jauregg might be the beginning of the study on the immunological concepts of schizophrenia. As the primary immune effector cells in the brain, microglia play...Background The well-known ‘pyrotherapy’ of Julius Wagner-Jauregg might be the beginning of the study on the immunological concepts of schizophrenia. As the primary immune effector cells in the brain, microglia play a pivotal role in neuroinfammatory processes. Maternal viral infection during pregnancy is associated with an increased risk for psychiatric disorders with presumed neurodevelopmental origin, including autism spectrum disorders and schizophrenia. The present study was to quantify microglia activation in vivo in the mature offspring of rats exposed to polyriboinosinic–polyribocytidilicacid （Poly I：C） during pregnancy using ^11C-PK11195 positron emission tomography （PET） and immunohistochemistry.Objective The study aimed to quantify microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly I：C exposed rats.Methods Offspring of Poly I：C-treated dams were the model group, offspring of saline-treated dams were the control group. Behavioural test for two groups was taken by spontaneous activity, prepulse inhibition （PPI） and latent inhibition （LI） test （including active avoidance conditioning task and passive avoidance conditioning task）. Randomly selected successful model rats were assessed by behavioural test in the model group and control group rats. 11C-PK11195 micro-PET/CT and immunohistochemistry were performed on the selected rats to measure microglia activation.Results The treatment group showed hyperlocomotion and defcits in PPI and LI compared with the control group. The treatment group also showed an increased 11C-PK11195 uptake ratio in the prefrontal cortex （t=-3.990, p=0.003） and hippocampus （t=-4.462, p=0.001）. The number of activated microglia cells was signifcantly higher in the treatment group than in the control group （hippocampus： t=8.204, p〈0.001; prefrontal： t=6.995, p〈0.001）. Within the treatment group, there were signifcant correlations between the behavioural parameters and the activation of microglia as measured by PET and immunohistochemistry.Conclusions The present study demonstrated microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly I：C exposed rats. This study suggests that microglia activation may play a possible or potential role in the pathogenesis of schizophrenia.展开更多
Using a modification of the very sensitive glucose-DAB-nickel(GDN)immunohistochemistry method,FOS(the expression protein of the oncogene c-fos)-,neuropeptide Y(NPY)-,somatostatin(SOM)-,leu-enkephalin(L-Enk),cholecysto...Using a modification of the very sensitive glucose-DAB-nickel(GDN)immunohistochemistry method,FOS(the expression protein of the oncogene c-fos)-,neuropeptide Y(NPY)-,somatostatin(SOM)-,leu-enkephalin(L-Enk),cholecystokinin(CCK)-,neurotensin(NT)-,and tyrosine hydroxylase(TH)-immunoreactivities were firstdemonstrated in the protozoan,Stylonychia mytilus.The GDN method and expression ofFOS,neuropeptides and TH in the Stylonychia mytilus were discussed.展开更多
The proper establishment of loop-vessel countercurrent arrangement in medulla provides the basis for formation of the concentrated urine.To date,the morphogenesis of it has not been fully discovered.The present study ...The proper establishment of loop-vessel countercurrent arrangement in medulla provides the basis for formation of the concentrated urine.To date,the morphogenesis of it has not been fully discovered.The present study investigated the spatial arrangement of nephron loop and vessel of developing kidneys using 3D visualization technique and immunohistochemistry for AQP-1 and NKCC2.Five-mm-thick serial paraffin sections were obtained from E14.5 and P5 kidneys and stained with hematoxylin,while 2.5-mm-thick serial epoxy sections were obtained from E17.5 and P56 kidneys and stained with toluidine blue.展开更多
现代肿瘤学认为肿瘤的发病与细胞凋亡数量的减少密切相关,第二个线粒体衍生的半胱氨酸蛋白酶激动剂（second mitochondria-derived activator of caspase,Smac）、X连锁凋亡抑制蛋白（X-linked inhibitor of apoptosis protein,XIAP）...现代肿瘤学认为肿瘤的发病与细胞凋亡数量的减少密切相关,第二个线粒体衍生的半胱氨酸蛋白酶激动剂（second mitochondria-derived activator of caspase,Smac）、X连锁凋亡抑制蛋白（X-linked inhibitor of apoptosis protein,XIAP）、半胱氨酸天冬氨酸蛋白酶3（cysteine containing aspartate specific protease,caspase-3）在细胞凋亡过程中起着重要的调控及效应作用。本研究采用免疫组化方法观察三者在鼻腔鼻窦鳞状细胞癌（nasal squamous cellcarcinoma,NSCC）及不同病理分期中的表达情况。展开更多
文摘The changes of carbamyl phosphate synthetase I(CPS 1)in diethylnitrosamine-(DEN)-inducedenzyme-altered liver cells were studied by means of immunohistochemical(PAP)and in situcDNA-mRNA hybridization methods.The experimental rats were treated with DEN,2-acetylaminofluorene(2-AAF)and 2/3 hepatectomy according to Solt-Farber’s protocol andwere further promoted by oral daily administration of 0.05% phenobarbital in drinking water.The results showed that the average number of lesions showing abnormal expression of CPS1 was relatively constant over the course of the experiment(8 months),while the numberof normally expressing lesions gradually decreased.The former lesions were also largerin volume than the latter ones.We conclude that in DEN-initiated lesions the abnormallyexpressed CPS 1 lesions may grow continuously,thus leading to the formation of largernodules.We also suspect that some of these lesions have increased tendencies to developinto tumors.
文摘One hundred and sixty-eight specimens of intestinal metaplasia(IM)with variousaccompanying lesions in gastric mucosa were studied with mucohistochemical and ABCimmunohistochemical staining,The quantitative analysis of cell DNA was done withflowcytometry for 36 specimens.The results indicated that the incidence of type Ⅱb IM wassignificantly higher in the groups of dysplasia(34.6％)and mucosa adjacent to gastric cancer(GC)(51.7％)than in the chronic atrophic gastritis(CAG)group(16.0％)(P【0.01).The expres-sion rate of monoclonal antibody MG7 related antigen(MG7-Ag)in type Ⅱb IM(473％)wasalso significantly higher than those in type la(29.7％),Ib(26.1％)and Ⅱa IM(28.3％)(P【0.05).Expression rate of MG7-Ag,DNA aneuploid rate and percentage of S phase cell werestatistically higher in the type Ⅱb IM with dysphsia(62.5％,62.5％ and 143±32)than in typeⅡb 1M without dysplasia(47.3％,12.5％and12.7±2.9)(P【0.05 and P【0.01).These findingssuppor the supposed progressive process:CAG→type Ⅱb IM→dysplasia→GC,andtype Ⅱb IM with dysplasia is closely associated with GC.
基金provided by the National Natural Science Foundation of China(No 81571318 to XQSNo 81371472 to LXL+5 种基金No 81401110 to XL)the Science and Technology Planning Project of Health and Family Planning Commission(No 201501015 to XQS)the International Science and Technology Cooperation Program of Henan(No 162102410061 to XQS)the Henan Province Union Fund Project(162300410275)the Zhengzhou University doctor team projectthe Youth Fund of the First Affiliated Hospital of Zhengzhou University(to XL and LJP)
文摘Background The well-known ‘pyrotherapy’ of Julius Wagner-Jauregg might be the beginning of the study on the immunological concepts of schizophrenia. As the primary immune effector cells in the brain, microglia play a pivotal role in neuroinfammatory processes. Maternal viral infection during pregnancy is associated with an increased risk for psychiatric disorders with presumed neurodevelopmental origin, including autism spectrum disorders and schizophrenia. The present study was to quantify microglia activation in vivo in the mature offspring of rats exposed to polyriboinosinic–polyribocytidilicacid （Poly I：C） during pregnancy using ^11C-PK11195 positron emission tomography （PET） and immunohistochemistry.Objective The study aimed to quantify microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly I：C exposed rats.Methods Offspring of Poly I：C-treated dams were the model group, offspring of saline-treated dams were the control group. Behavioural test for two groups was taken by spontaneous activity, prepulse inhibition （PPI） and latent inhibition （LI） test （including active avoidance conditioning task and passive avoidance conditioning task）. Randomly selected successful model rats were assessed by behavioural test in the model group and control group rats. 11C-PK11195 micro-PET/CT and immunohistochemistry were performed on the selected rats to measure microglia activation.Results The treatment group showed hyperlocomotion and defcits in PPI and LI compared with the control group. The treatment group also showed an increased 11C-PK11195 uptake ratio in the prefrontal cortex （t=-3.990, p=0.003） and hippocampus （t=-4.462, p=0.001）. The number of activated microglia cells was signifcantly higher in the treatment group than in the control group （hippocampus： t=8.204, p〈0.001; prefrontal： t=6.995, p〈0.001）. Within the treatment group, there were signifcant correlations between the behavioural parameters and the activation of microglia as measured by PET and immunohistochemistry.Conclusions The present study demonstrated microglia activation in vivo in the prefrontal cortex and hippocampus in mature offspring of prenatal Poly I：C exposed rats. This study suggests that microglia activation may play a possible or potential role in the pathogenesis of schizophrenia.
文摘Using a modification of the very sensitive glucose-DAB-nickel(GDN)immunohistochemistry method,FOS(the expression protein of the oncogene c-fos)-,neuropeptide Y(NPY)-,somatostatin(SOM)-,leu-enkephalin(L-Enk),cholecystokinin(CCK)-,neurotensin(NT)-,and tyrosine hydroxylase(TH)-immunoreactivities were firstdemonstrated in the protozoan,Stylonychia mytilus.The GDN method and expression ofFOS,neuropeptides and TH in the Stylonychia mytilus were discussed.
文摘The proper establishment of loop-vessel countercurrent arrangement in medulla provides the basis for formation of the concentrated urine.To date,the morphogenesis of it has not been fully discovered.The present study investigated the spatial arrangement of nephron loop and vessel of developing kidneys using 3D visualization technique and immunohistochemistry for AQP-1 and NKCC2.Five-mm-thick serial paraffin sections were obtained from E14.5 and P5 kidneys and stained with hematoxylin,while 2.5-mm-thick serial epoxy sections were obtained from E17.5 and P56 kidneys and stained with toluidine blue.
文摘现代肿瘤学认为肿瘤的发病与细胞凋亡数量的减少密切相关,第二个线粒体衍生的半胱氨酸蛋白酶激动剂（second mitochondria-derived activator of caspase,Smac）、X连锁凋亡抑制蛋白（X-linked inhibitor of apoptosis protein,XIAP）、半胱氨酸天冬氨酸蛋白酶3（cysteine containing aspartate specific protease,caspase-3）在细胞凋亡过程中起着重要的调控及效应作用。本研究采用免疫组化方法观察三者在鼻腔鼻窦鳞状细胞癌（nasal squamous cellcarcinoma,NSCC）及不同病理分期中的表达情况。
