目的:第10号染色体同源丢失性磷酸酶-张力蛋白基因(Phosphatase and tension homology deleted on chromosometen,PTEN)、基质金属蛋白酶(Matrix metalloproteinase9,MMP-9)、激酶抑制蛋白(Kinase inhibited protein1,P27kip1)在乳腺癌...目的:第10号染色体同源丢失性磷酸酶-张力蛋白基因(Phosphatase and tension homology deleted on chromosometen,PTEN)、基质金属蛋白酶(Matrix metalloproteinase9,MMP-9)、激酶抑制蛋白(Kinase inhibited protein1,P27kip1)在乳腺癌中的表达及其相关性,探讨三者的表达与临床分期、淋巴结转移及预后的关系。方法:选取50例有完整临床及病理资料的乳腺癌病例,采用SP免疫组织化学技术检测乳腺癌中PTEN、MMP-9、P27kip1蛋白的表达情况。结果:PTEN、MMP-9、P27kip1在乳腺癌中高表达率分别为54%、74%、62%。结论:PTEN、MMP-9、P27kip1在乳腺癌中的表达与患者的临床分期及淋巴结转移有关,可作为乳腺癌预后的参考指标。展开更多
目的探讨视网膜母细胞瘤结合蛋白4(retinoblastoma binding protein 4,RBBP4)和BCL-2在胃癌中的表达及其与临床病理特征的关系。方法采用免疫组化SP法检测72例胃癌及对应癌旁组织中RBBP4、BCL-2蛋白表达,分析RBBP4与胃癌临床病理特征的...目的探讨视网膜母细胞瘤结合蛋白4(retinoblastoma binding protein 4,RBBP4)和BCL-2在胃癌中的表达及其与临床病理特征的关系。方法采用免疫组化SP法检测72例胃癌及对应癌旁组织中RBBP4、BCL-2蛋白表达,分析RBBP4与胃癌临床病理特征的关系及与BCL-2表达的相关性。实时荧光定量PCR法检测20例胃癌及癌旁组织中RBBP4 mRNA的表达。结果 RBBP4、BCL-2蛋白在癌组织中的阳性率分别为55.6%(40/72)、50%(36/72),显著高于癌旁组织13.9%、16.7%。RBBP4和BCL-2高表达均与肿瘤分化、浸润深度及淋巴结转移明显相关(P<0.05),且RBBP4和BCL-2蛋白的表达呈正相关(r=0.559,P<0.05)。RBBP4 mRNA在胃癌组织中的相对表达水平(9.988±1.948)显著高于癌旁组织(1.458±0.489)。结论RBBP4在胃癌中的表达升高,可能和胃癌的发生、发展及侵袭转移有关,可能成为胃癌诊断和预后评估的新指标。展开更多
The work addresses to the study of the molecular and crystal structure and properties of a new energyintensive compound 3,6-bis(2,2,2-trinitroethylnitramino)-1,2,4,5-tetrazine(NBTAT),first obtained by the authors in 2...The work addresses to the study of the molecular and crystal structure and properties of a new energyintensive compound 3,6-bis(2,2,2-trinitroethylnitramino)-1,2,4,5-tetrazine(NBTAT),first obtained by the authors in 2020.NBTAT compound crystallizes in the monoclinic system,space group P2(1)/n,density at room temperature 1.939 g/cm^(3).The energies of crystal packing and pairwise intermolecular interactions in NBTAT and its unnitrated analogue BTAT were calculated,and their comparative analysis was carried out.The enthalpy of formation of NBTAT molecules was calculated by quantum-chemical methods using Gaussian 09,and the enthalpy of formation of NBTAT in the solid phase(618 kJ/mol)was estimated.The energy capabilities of NBTAT as an oxidizer of solid composite propellants are estimated.It is shown that in metal-free compositions NBTAT is significantly superior to ammonium perchlorate(AP),dinitramide ammonium salt(ADN),HMX,BTAT at all stages of rocket systems,and is comparable to the superdense CL-20 yielding to the latter at the lower stages and slightly winning at the upper stages.展开更多
文摘目的:第10号染色体同源丢失性磷酸酶-张力蛋白基因(Phosphatase and tension homology deleted on chromosometen,PTEN)、基质金属蛋白酶(Matrix metalloproteinase9,MMP-9)、激酶抑制蛋白(Kinase inhibited protein1,P27kip1)在乳腺癌中的表达及其相关性,探讨三者的表达与临床分期、淋巴结转移及预后的关系。方法:选取50例有完整临床及病理资料的乳腺癌病例,采用SP免疫组织化学技术检测乳腺癌中PTEN、MMP-9、P27kip1蛋白的表达情况。结果:PTEN、MMP-9、P27kip1在乳腺癌中高表达率分别为54%、74%、62%。结论:PTEN、MMP-9、P27kip1在乳腺癌中的表达与患者的临床分期及淋巴结转移有关,可作为乳腺癌预后的参考指标。
基金The Ministry of Science and Higher Education of the Russian Federation(Agreement with Zelinsky Institute of Organic Chemistry RAS No 075-15-2020-803).
文摘The work addresses to the study of the molecular and crystal structure and properties of a new energyintensive compound 3,6-bis(2,2,2-trinitroethylnitramino)-1,2,4,5-tetrazine(NBTAT),first obtained by the authors in 2020.NBTAT compound crystallizes in the monoclinic system,space group P2(1)/n,density at room temperature 1.939 g/cm^(3).The energies of crystal packing and pairwise intermolecular interactions in NBTAT and its unnitrated analogue BTAT were calculated,and their comparative analysis was carried out.The enthalpy of formation of NBTAT molecules was calculated by quantum-chemical methods using Gaussian 09,and the enthalpy of formation of NBTAT in the solid phase(618 kJ/mol)was estimated.The energy capabilities of NBTAT as an oxidizer of solid composite propellants are estimated.It is shown that in metal-free compositions NBTAT is significantly superior to ammonium perchlorate(AP),dinitramide ammonium salt(ADN),HMX,BTAT at all stages of rocket systems,and is comparable to the superdense CL-20 yielding to the latter at the lower stages and slightly winning at the upper stages.
