A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented.Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels ]via] photopolymer...A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented.Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels ]via] photopolymerization in a polyacrylamide matrix.The resulting polymeric,hydrogel plugs are porous under electrophoretic conditions,and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA.The total analysis process can be completed within 5 min,and the limit of detection is 0.1 μmol/L.This method is simple,rapid and feasible.The double-stranded DNA can be chemically denatured,and the chip is reusable.The conditions for photopolymerization,hybridization,and denaturation were discussed as well.展开更多
DNA水凝胶是具有三维聚合物网络的高保水性材料。研究人员设计了多种DNA水凝胶交联制备方法,并通过向其中引入其他功能分子或与其他类型的功能材料相互结合,构建了具有优异性能的DNA水凝胶,受到了广泛关注。适配体是基于指数富集的配体...DNA水凝胶是具有三维聚合物网络的高保水性材料。研究人员设计了多种DNA水凝胶交联制备方法,并通过向其中引入其他功能分子或与其他类型的功能材料相互结合,构建了具有优异性能的DNA水凝胶,受到了广泛关注。适配体是基于指数富集的配体系统进化(systematic evolution of ligands by exponential enrichment,SELEX)技术从随机寡核苷酸文库中筛选获得的对目标物质具有良好特异性与亲和力的寡核苷酸序列。适配体功能化的DNA水凝胶具有靶向范围广、稳定性好、易于修饰、操作简单和成本低等优点,得到了广泛应用。本文概述了构建适配体功能化的DNA水凝胶的基本设计原则与分类,重点介绍了适配体功能化的DNA水凝胶在食品安全检测中的最新策略,最后,讨论了适配体功能化的DNA水凝胶面临的挑战以及对未来的展望,旨在为其在食品安全领域的应用提供参考。展开更多
文摘A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented.Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels ]via] photopolymerization in a polyacrylamide matrix.The resulting polymeric,hydrogel plugs are porous under electrophoretic conditions,and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA.The total analysis process can be completed within 5 min,and the limit of detection is 0.1 μmol/L.This method is simple,rapid and feasible.The double-stranded DNA can be chemically denatured,and the chip is reusable.The conditions for photopolymerization,hybridization,and denaturation were discussed as well.
文摘DNA水凝胶是具有三维聚合物网络的高保水性材料。研究人员设计了多种DNA水凝胶交联制备方法,并通过向其中引入其他功能分子或与其他类型的功能材料相互结合,构建了具有优异性能的DNA水凝胶,受到了广泛关注。适配体是基于指数富集的配体系统进化(systematic evolution of ligands by exponential enrichment,SELEX)技术从随机寡核苷酸文库中筛选获得的对目标物质具有良好特异性与亲和力的寡核苷酸序列。适配体功能化的DNA水凝胶具有靶向范围广、稳定性好、易于修饰、操作简单和成本低等优点,得到了广泛应用。本文概述了构建适配体功能化的DNA水凝胶的基本设计原则与分类,重点介绍了适配体功能化的DNA水凝胶在食品安全检测中的最新策略,最后,讨论了适配体功能化的DNA水凝胶面临的挑战以及对未来的展望,旨在为其在食品安全领域的应用提供参考。
