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Potential Application of Pseudomonas hunanensis MGJ-2 for Biodegradation of Nicotine in Tobacco Plants
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作者 Yu Tao Li Xu +5 位作者 Liu Xinbo Huang Yaning Wang Jiaqi Yu Qingyue Liu Rongmei Li Haitao 《Journal of Northeast Agricultural University(English Edition)》 2025年第3期45-53,共9页
Nicotine,also known as nicotinic norephedrine,is one of the main alkaloids present in tobacco plants.In recent years,due to the increase in tobacco production and smoking population,the environmental and health issues... Nicotine,also known as nicotinic norephedrine,is one of the main alkaloids present in tobacco plants.In recent years,due to the increase in tobacco production and smoking population,the environmental and health issues caused by nicotine have become increasingly severe.Traditional methods have proven ineffective in efficiently degrading residual nicotine.To address this issue,scientists both domestically and internationally have turned to biodegradation methods to tackle the environmental and health problems caused by residual nicotine.In this study,an enrichment method was used to screen bacteria with nicotine-degrading capabilities from the soil of tobacco planting sites at the Tobacco Research Institute of Heilongjiang in Bin County,Harbin City.Through phenotypic observations and 16S rDNA identification,a bacterial strain identified as Pseudomonas hunanensis MGJ-2 was isolated,capable of utilizing nicotine as a carbon and nitrogen source for growth.High-performance liquid chromatography(HPLC)-1 analysis revealed that within 25 h,strain MGJ-2 could degrade nicotine 500 mg·L^(-1) with an efficiency exceeding 99.9%.Strain MGJ-2 was applied to tobacco,and after 15 days of incubation and fermentation,it degraded 10.57%of nicotine in tobacco.Overall,the discovery of strain MGJ-2 enriched the resources of nicotine-degrading strains.Its remarkable biodegradation performance held immense potential for future biodegradation of nicotine in tobacco. 展开更多
关键词 Pseudomonas hunanensis MGJ-2 high-performance liquid chromatography(HPLC) degradation characteristic nicotine degradation
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Improved determination of salicylaldoxime in water samples by liquid-liquid extraction followed by high performance liquid chromatographic analysis 被引量:3
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作者 KONG Qian BAI Xiu-zhi +2 位作者 LIN Hui-ju LI Hai-pu YANG Zhao-guang 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第4期701-708,共8页
For the determination of salicylaldoxime in environmental water samples,a stable and rapid method with low detection was proposed and established,based on the liquid-liquid extraction-high performance liquid chromatog... For the determination of salicylaldoxime in environmental water samples,a stable and rapid method with low detection was proposed and established,based on the liquid-liquid extraction-high performance liquid chromatography with ultraviolet detector.Parameters including extraction solvent,ionic strength,solution pH,and extraction pattern were discussed for the optimal quantification of salicylaldoxime-spiked water.When the described method was applied to four spiked water samples,the obtained average extraction recovery rate was found between 87%–107%and relative standard deviation was below 6%.At the same time,good linear relationships were observed for spiked water samples from 0.01 to 10μg/mL(R2=0.9993).In addition,the detection limit of salicylaldoxime was revealed between 0.003–0.008μg/mL,which is two orders of magnitude lower than previously reported results.Thus,the presented method may be advantageous for the high-efficiency determination of salicylaldoxime in water samples. 展开更多
关键词 high-performance liquid chromatography liquid-liquid extraction SALICYLALDOXIME
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Determination of urinary 3-methylhistidine by highperformance liquid chromatography with o-phthaldialdehyde pre-column derivatization
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作者 沈敏 袁倚盛 尹路 《医学研究生学报》 CAS 1992年第3期271-275,共5页
A high-performance liquid chromatography was describ-ed to measure the concentration of human 3-methylhistidine in urine witho-phthaldialdehyde pre-column derivatization.This assay system resultedin a clear separation... A high-performance liquid chromatography was describ-ed to measure the concentration of human 3-methylhistidine in urine witho-phthaldialdehyde pre-column derivatization.This assay system resultedin a clear separation of 3-methylhistidine derivative from other derivativ-es in urine within 25 min.Linearity,recovery and coefficients of vari-ation were studied.The method has been successfully applied to routineanalysis for clinical studies and diagnosis. 展开更多
关键词 3-methylhistidine high-performance liquid CHROMATOGRAPHY
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反相高效液相色谱法测定青荚叶中表儿茶素含量 被引量:8
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作者 刘利娥 李红萍 +3 位作者 吴予明 刘洁 于斐 张洪权 《郑州大学学报(医学版)》 CAS 北大核心 2010年第1期132-134,共3页
目的:采用反相高效液相色谱法建立青荚叶中表儿茶素含量的测定方法。方法:用甲醇-水(体积比1:1)为溶剂,超声波法提取青荚叶中的表儿茶素。采用反相高效液相色谱法。色谱条件为:色谱柱:ZorbaxXDB-C18柱(4.6mm×250mm,5μm);流动相组... 目的:采用反相高效液相色谱法建立青荚叶中表儿茶素含量的测定方法。方法:用甲醇-水(体积比1:1)为溶剂,超声波法提取青荚叶中的表儿茶素。采用反相高效液相色谱法。色谱条件为:色谱柱:ZorbaxXDB-C18柱(4.6mm×250mm,5μm);流动相组成:流动相A,400mL0.04mol/L柠檬酸+80mLN,N-二甲基甲酰胺+20mL四氢呋喃;流动相B,甲醇;流动相的洗脱方式为等强度洗脱:流动相A+B(体积比85:15);流速1.0mL/min;柱温:35℃;检测器为二极管阵列检测器,检测波长280nm。采用外标法定量。结果:表儿茶素线性范围为0~364.00mg/L(r=0.9999),平均回收率为98.4%,RSD为2.08%(n=3)。青荚叶中表儿茶素含量为(5.334±0.111)mg/g。结论:该方法简便、准确且灵敏,适用于青荚叶中表儿茶素含量测定。 展开更多
关键词 青荚叶 高效液相色谱法 表儿茶素
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降钙素基因相关肽的降解 被引量:4
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作者 李春英 李粤 汤健 《北京医科大学学报》 CSCD 1989年第4期286-288,共3页
本研究应用^(125)Ⅰ-CGRP体外实验结合高效液相分析的方法,探讨了CGRP在血浆及肝、肺、肾匀浆上清液中的降解。结果表明CGRP在肝匀浆上清液中降解最快,在血浆中代谢缓慢,肽酶抑制剂可以部分抑制CGRP在肺匀浆上清液中的降解。
关键词 降钙素 基因相关肽 色谱法
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Chromatographic fingerprint analysis of Fructus Aurantii Immaturus by HPLC-DAD and chemometric methods 被引量:4
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作者 徐小娜 蒋军辉 +3 位作者 梁逸曾 李晓如 易伦朝 成金乐 《Journal of Central South University》 SCIE EI CAS 2011年第2期353-360,共8页
An efficient method for quality control of Fructus Aurantii Immaturus (FAI),a famous traditional Chinese medicine (TCM) was established. A simple and reliable high-performance liquid chromatography-photodiode array de... An efficient method for quality control of Fructus Aurantii Immaturus (FAI),a famous traditional Chinese medicine (TCM) was established. A simple and reliable high-performance liquid chromatography-photodiode array detector (HPLC-DAD) procedure coupled with chemometric methods was developed for fingerprint analysis,qualitative analysis and quantitative determination of this herb. In qualitative and quantitative analyses,heuristic evolving latent projection (HELP) method was employed to resolve the overlapping peaks of the tested samples. Two bioactive components,namely hesperidin and naringin,are confirmed and determined,together with four flavonoids compounds tentatively identified including two new ones. From fingerprint analysis,the fingerprint data were processed with correlation coefficients for quantitative expression of their similarity and dissimilarity. The developed method based on an integration of chromatographic fingerprint and quantitative analysis is scientific,and the obtained results can be applied to the quality control of herb medicine. 展开更多
关键词 FINGERPRINT quality control Fructus Aurantii Immaturus high-performance liquid chromatography heuristic evolvinglatent projection method HESPERIDIN NARINGIN
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Cloud-point extraction and preconcentration of bisphenol A from water samples 被引量:2
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作者 谭志坚 李芬芳 《Journal of Central South University》 SCIE EI CAS 2012年第8期2136-2141,共6页
A novel cloud-point extraction (CPE) was successfully used in preconcentration of biphenol A (BPA) from aqueous solutions. Majority of BPA is extracted into the surfactant-rich phase. The parameters affecting the ... A novel cloud-point extraction (CPE) was successfully used in preconcentration of biphenol A (BPA) from aqueous solutions. Majority of BPA is extracted into the surfactant-rich phase. The parameters affecting the CPE such as concentration of surfactant and electrolyte, equilibration temperature and time and pH of sample solution were investigated. The samples were analyzed by high-performance liquid chromatography with ultraviolet detection. Under the optimized conditions, preconcentration of 10 mL sample gives a preconcentration factor of 11. The limit of detection (LOD) and limit of quantification (LOQ) are 0.1 μg/L and 0.33 μg/L, respectively. The linear range of the proposed method is 0.2-20 μg/L with correlation coefficients greater than 0.998 7 and the spiking recove6es are 97.96%-100.42%. The interference factor was tested and the extraction mechanism was also investigated. Thus, the developed CPE has proven to be an efficient, green, rapid and inexpensive approach for extraction and preconcentration of BPA from water samples. 展开更多
关键词 biphenol A cloud-point extraction extraction mechanism high-performance liquid chromatography water samples
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新型吡咯烷键合反相高效液相色谱固定相的制备与研究(英文)
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作者 Elahe NAGHDI Kourosh TABAR HEYDAR +1 位作者 Ali SHARIFI Seyyed Hamid AHMADI 《色谱》 CAS CSCD 北大核心 2017年第11期1120-1128,共9页
A new ionic liquid-based high-performance liquid chromatography stationary phase is reported.A derivative of N-methyl pyrrolidinium tetrafluoroborate was covalently immobilized on the surface of silica particles to pr... A new ionic liquid-based high-performance liquid chromatography stationary phase is reported.A derivative of N-methyl pyrrolidinium tetrafluoroborate was covalently immobilized on the surface of silica particles to prepare silica-based N-methyl pyrrolidinium tetrafluoroborate(SilprMP BF4)stationary phase.The obtained ionic liquid-modified silica was evaluated and confirmed by elemental analysis,infrared spectroscopy,and thermogravimetric analysis.A column was packed with the modified particles.The retention behavior of aromatic compounds,alkyl benzenes,and acidic and basic compounds on the SilprMP BF4 stationary phase was studied under reversed-phase liquid chromatography conditions.The effect of the eluent pH on the separation of the acidic and basic compounds was also studied.The new stationary phase involves multiple retention mechanisms,such as electrostatic,hydrophobic,ion-dipole,and anion-exchange interactions,which might lead to multipurpose separation media. 展开更多
关键词 REVERSED-PHASE high-performance liquid CHROMATOGRAPHY stationary phase IONIC liquids (ILs) PYRROLIDINIUM
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