OBJECTIVE To investigate regulatory effects of hyperoside(Hyp)on IP3/PKC/TRPV4 pathway in rat cerebral basilar artery(CBA)subjected to global cerebral ischemia-reperfusion(I/R).METHODS The model of global cerebral I/R...OBJECTIVE To investigate regulatory effects of hyperoside(Hyp)on IP3/PKC/TRPV4 pathway in rat cerebral basilar artery(CBA)subjected to global cerebral ischemia-reperfusion(I/R).METHODS The model of global cerebral I/R in rats was established by four-vessel occlusions methods.The treated rats were administrated with Hyp(50 mg·kg^-1)group,Hyp(50 mg·kg^-1)+HC-067047(10 mg·kg^-1),Hyp(50 mg·kg^-1)+2APB(2 mg·kg^-1),Hyp(50 mg·kg^-1)+BisI(2.5 mg·kg^-1),Hyp(50 mg·kg^-1)+2APB(2 mg·kg^-1)+BisI(2.5 mg·kg^-1).Hematoxylin-eosin(HE)and Nissl staining were performed and the contents of methane dicarboxylic aldehyde(MDA),neuron-specific enolase(NSE),S100β and the activity of lactic dehydrogenase(LDH)in serum were measured by enzyme-linked immunosorbnent assay(ELISA).The specific blocker N-nitro-L-arginine-methyl-ester(L-NAME)and indomethacin(Indo)were used to delete the prostacyclin(PGI2)and nitric oxide(NO)dependent relaxation.The protein expression level of TRPV4 was detected by Western blotting.Ca2+intensity in vascular smooth muscle cells was measured by confocal laser scanning microscope and flow cytometry was performed to observe the apoptosis of CBA endothelial cells after in vivo administration.RESULTS Hyp induced a dose-dependent relaxation of CBA in IR rats via a PGI2 and NO independent manner,as evidenced by alleviated patho⁃logical changes and up-regulated expression of TRPV4 protein in the endothelial cells from cerebral vessels.Hyp signifi⁃cantly reduced the contents of MDA,NSE,S100βand the activity of LDH in serum and decreased the fluorescence intensity of Ca2+in cerebral vascular smooth muscle cells by in vivo administration.The apoptotic rate of endothelial cells in Hyp treated group was significantly less than that in IR group.CONCLUSION Hyp does in fact ameliorate I/R injury by regulatingIP3/PKC/TRPV4 pathway.展开更多
OBJECTIVE The total steroid saponins(TSSN)isolated from Dioscorea zingiberensis C.H.Wright(D.zingiberensis)has shown a variety of beneficial bioactivities.However,there are no reports about the neuroprotective effects...OBJECTIVE The total steroid saponins(TSSN)isolated from Dioscorea zingiberensis C.H.Wright(D.zingiberensis)has shown a variety of beneficial bioactivities.However,there are no reports about the neuroprotective effects of the TSSN until now.Therefore,we explored the neuroprotective effects of TSSN on rats against transient focal cerebral ischemia-reperfusion(I/R)and the underlying mechanisms.METHODS The healthy adult Sprague-Dawley rats were randomly assigned into six groups.After pre-treatment with the TSSN intragastrically for six days,the rats were subjected to the ischemia injury by the surgery of middle cerebral artery occlusion(MCAO)for 90 min.Some indexes were evaluated and detected.RESULTS As compared to the I/R group,TSSN group of rats,especially given the 30 mg·kg^-1 of TSSN,not only marked reduction in the neurological deficit scores,cerebral infarct volume,and brain edema,but also an increase in neuron survival(Nissl bodies)in the hippocampal cornuammons 1(CA1)and cortex hemisphere of the ipsilateral ischemia.At the same time,the inflammatory cytokines in serum induced by MCAO were significantly alleviated by the TSSN pre-administration.What′s more,the increase of caspase-3 was evidently reduced in the CA1 and cortex of the hemisphere injured brain.Finally,the down-regulating anti-apoptotic Bcl-2 and up-regulating pro-apoptotic Bax proteins were obviously suppressed.CONCLUSION TSSN plays a potential neuroprotective role against a severe injury induced by transient focal cerebral ischemic reperfusion in a rat experimental model,and this role may be mediated by its antiinflammatory and anti-apoptotic actions.展开更多
目的探讨远隔缺血适应(RIC)相关领域的国际研究热点和趋势。方法选取Web of Science数据库核心集合为数据来源,检索2000年1月1日至2024年12月12日发表的RIC相关英文文献。通过Microsoft Excel、CiteSpace、VOSviewer软件对检索的文献进...目的探讨远隔缺血适应(RIC)相关领域的国际研究热点和趋势。方法选取Web of Science数据库核心集合为数据来源,检索2000年1月1日至2024年12月12日发表的RIC相关英文文献。通过Microsoft Excel、CiteSpace、VOSviewer软件对检索的文献进行文献计量学及可视化分析,对年发表文献数量、国家、机构、出版期刊、作者、被引参考文献、关键词及关键词突现等信息进行分析。结果(1)2000年1月1日至2024年12月12日发表的RIC相关论著和综述共1316篇,由来自59个国家1597个机构的6688名作者发表在456种期刊上,引用了来自3860种期刊的32064篇文献。近10年RIC相关文献发表量均较高,其中2017年发表文献数量最多,为145篇。(2)发表RIC相关文献数量最多的国家为中国(448篇),其次为美国(240篇),各国间合作广泛。发表RIC相关文献最多的机构为中国首都医科大学(103篇),其次为丹麦奥胡斯大学(63篇),各发文机构间合作紧密。发表RIC相关文献数量排名前3位的期刊为Plos One(37篇)、Basic Research in Cardiology(32篇)和Journal of Surgical Research(26篇)。发表RIC相关文献数量前3位的作者为吉训明(60篇)、Heusch G(30篇)和任长虹(29篇)。(3)RIC相关文献的参考文献中被引次数最多的文献为Preconditioning with ischemia:a delay of lethal cell injury in ischemic myocardium(414次)。被引参考文献发表于3860种期刊,被引次数最多的期刊为Circulation(3933次引用)。(4)共提取4089个关键词,出现次数前3位的关键词分别为“reperfusion injury(再灌注损伤)”“remote ischemic preconditioning(远隔缺血预适应)”和“cardioprotection(心脏保护)”。突现强度前3位的关键词分别为“remote ischemic conditioning(远隔缺血预适应)”“ischemic stroke(缺血性卒中)”和“thrombolysis(溶栓)”。近5年突现强度较高的关键词为“thrombolysis(溶栓)”“international clinical recommendations(国际临床建议)”“safety(安全性)”“ischemic stroke(缺血性卒中)”“recovery(恢复)”和“efficacy(疗效)”。结论近10年RIC相关研究的文献保持较高发文量,各国家、机构、学者间合作紧密,中国首都医科大学在国际RIC相关研究中处于重要地位。RIC相关研究主要关注缺血-再灌注保护的作用机制、心脏保护和缺血性卒中等方面,RIC在缺血性卒中方面的临床应用、疗效及安全性为国际RIC研究的趋势和热点。展开更多
基金National Natural Science Foundation of China(81173596)Natural Science Foundation of the Department of Education of Anhui Province(KJ2015A157)
文摘OBJECTIVE To investigate regulatory effects of hyperoside(Hyp)on IP3/PKC/TRPV4 pathway in rat cerebral basilar artery(CBA)subjected to global cerebral ischemia-reperfusion(I/R).METHODS The model of global cerebral I/R in rats was established by four-vessel occlusions methods.The treated rats were administrated with Hyp(50 mg·kg^-1)group,Hyp(50 mg·kg^-1)+HC-067047(10 mg·kg^-1),Hyp(50 mg·kg^-1)+2APB(2 mg·kg^-1),Hyp(50 mg·kg^-1)+BisI(2.5 mg·kg^-1),Hyp(50 mg·kg^-1)+2APB(2 mg·kg^-1)+BisI(2.5 mg·kg^-1).Hematoxylin-eosin(HE)and Nissl staining were performed and the contents of methane dicarboxylic aldehyde(MDA),neuron-specific enolase(NSE),S100β and the activity of lactic dehydrogenase(LDH)in serum were measured by enzyme-linked immunosorbnent assay(ELISA).The specific blocker N-nitro-L-arginine-methyl-ester(L-NAME)and indomethacin(Indo)were used to delete the prostacyclin(PGI2)and nitric oxide(NO)dependent relaxation.The protein expression level of TRPV4 was detected by Western blotting.Ca2+intensity in vascular smooth muscle cells was measured by confocal laser scanning microscope and flow cytometry was performed to observe the apoptosis of CBA endothelial cells after in vivo administration.RESULTS Hyp induced a dose-dependent relaxation of CBA in IR rats via a PGI2 and NO independent manner,as evidenced by alleviated patho⁃logical changes and up-regulated expression of TRPV4 protein in the endothelial cells from cerebral vessels.Hyp signifi⁃cantly reduced the contents of MDA,NSE,S100βand the activity of LDH in serum and decreased the fluorescence intensity of Ca2+in cerebral vascular smooth muscle cells by in vivo administration.The apoptotic rate of endothelial cells in Hyp treated group was significantly less than that in IR group.CONCLUSION Hyp does in fact ameliorate I/R injury by regulatingIP3/PKC/TRPV4 pathway.
基金National Natural Science Foundation of China(81603267)Fundamental Research Funds for the Central UniversitiesShaanxi Key Laboratory of "Qiyao"Resources and Anti-tumor Activities
文摘OBJECTIVE The total steroid saponins(TSSN)isolated from Dioscorea zingiberensis C.H.Wright(D.zingiberensis)has shown a variety of beneficial bioactivities.However,there are no reports about the neuroprotective effects of the TSSN until now.Therefore,we explored the neuroprotective effects of TSSN on rats against transient focal cerebral ischemia-reperfusion(I/R)and the underlying mechanisms.METHODS The healthy adult Sprague-Dawley rats were randomly assigned into six groups.After pre-treatment with the TSSN intragastrically for six days,the rats were subjected to the ischemia injury by the surgery of middle cerebral artery occlusion(MCAO)for 90 min.Some indexes were evaluated and detected.RESULTS As compared to the I/R group,TSSN group of rats,especially given the 30 mg·kg^-1 of TSSN,not only marked reduction in the neurological deficit scores,cerebral infarct volume,and brain edema,but also an increase in neuron survival(Nissl bodies)in the hippocampal cornuammons 1(CA1)and cortex hemisphere of the ipsilateral ischemia.At the same time,the inflammatory cytokines in serum induced by MCAO were significantly alleviated by the TSSN pre-administration.What′s more,the increase of caspase-3 was evidently reduced in the CA1 and cortex of the hemisphere injured brain.Finally,the down-regulating anti-apoptotic Bcl-2 and up-regulating pro-apoptotic Bax proteins were obviously suppressed.CONCLUSION TSSN plays a potential neuroprotective role against a severe injury induced by transient focal cerebral ischemic reperfusion in a rat experimental model,and this role may be mediated by its antiinflammatory and anti-apoptotic actions.
文摘目的探讨远隔缺血适应(RIC)相关领域的国际研究热点和趋势。方法选取Web of Science数据库核心集合为数据来源,检索2000年1月1日至2024年12月12日发表的RIC相关英文文献。通过Microsoft Excel、CiteSpace、VOSviewer软件对检索的文献进行文献计量学及可视化分析,对年发表文献数量、国家、机构、出版期刊、作者、被引参考文献、关键词及关键词突现等信息进行分析。结果(1)2000年1月1日至2024年12月12日发表的RIC相关论著和综述共1316篇,由来自59个国家1597个机构的6688名作者发表在456种期刊上,引用了来自3860种期刊的32064篇文献。近10年RIC相关文献发表量均较高,其中2017年发表文献数量最多,为145篇。(2)发表RIC相关文献数量最多的国家为中国(448篇),其次为美国(240篇),各国间合作广泛。发表RIC相关文献最多的机构为中国首都医科大学(103篇),其次为丹麦奥胡斯大学(63篇),各发文机构间合作紧密。发表RIC相关文献数量排名前3位的期刊为Plos One(37篇)、Basic Research in Cardiology(32篇)和Journal of Surgical Research(26篇)。发表RIC相关文献数量前3位的作者为吉训明(60篇)、Heusch G(30篇)和任长虹(29篇)。(3)RIC相关文献的参考文献中被引次数最多的文献为Preconditioning with ischemia:a delay of lethal cell injury in ischemic myocardium(414次)。被引参考文献发表于3860种期刊,被引次数最多的期刊为Circulation(3933次引用)。(4)共提取4089个关键词,出现次数前3位的关键词分别为“reperfusion injury(再灌注损伤)”“remote ischemic preconditioning(远隔缺血预适应)”和“cardioprotection(心脏保护)”。突现强度前3位的关键词分别为“remote ischemic conditioning(远隔缺血预适应)”“ischemic stroke(缺血性卒中)”和“thrombolysis(溶栓)”。近5年突现强度较高的关键词为“thrombolysis(溶栓)”“international clinical recommendations(国际临床建议)”“safety(安全性)”“ischemic stroke(缺血性卒中)”“recovery(恢复)”和“efficacy(疗效)”。结论近10年RIC相关研究的文献保持较高发文量,各国家、机构、学者间合作紧密,中国首都医科大学在国际RIC相关研究中处于重要地位。RIC相关研究主要关注缺血-再灌注保护的作用机制、心脏保护和缺血性卒中等方面,RIC在缺血性卒中方面的临床应用、疗效及安全性为国际RIC研究的趋势和热点。
