OBJECTIVE To investigate the compatibility of the prescription Rhodiolarosea,Cordycepsmilitaris,and Rhubarb root.METHODS The DIO mice accompanying with insulin resistance,hypercholesterolemia and obesity were induced ...OBJECTIVE To investigate the compatibility of the prescription Rhodiolarosea,Cordycepsmilitaris,and Rhubarb root.METHODS The DIO mice accompanying with insulin resistance,hypercholesterolemia and obesity were induced by high fat diet(HFD)in C57BL/6mice.For evaluating the insulin sensitivity in vivo,the values of serum insulin,HOMA-IR,and the glucose infuse rate(GIR)in hyperinsulinemiceuglycemic clamp test were determined;the time-dependent blood glucose changes were measured after insulin loading in insulin tolerance test(ITT),or after glucose loading in glucose tolerance test(GTT),respectively.Recombinant human protein tyrosine phosphatase 1B(PTP1B)was overexpressed by hGST-PTP1BBL21 E.coli and purified by GST affinity chromatography.The activities of PTP1 Band glutamine:fructose 6-phosphate amidotransferase(GFAT)were measured by the pNPP and GDH method,respectively.The activity ofα-glucosidase was determined with the substrates of p-nitrophenyl-alpha-D-glucopyranoside(pNPG).The IC50 value was calculated using the software GraphPad Prism 5.RESULTS 1st,the standard for the raw materials and for the extraction process of Rhodiolarosea,Cordycepsmilitaris,and Rhubarbroot are formulated according to the inhibition on PTP1 B,α-glucosidase,and GFAT in vitro,and the improvement on impaired glucose tolerance(IGT),hypercholesterolemia,and obesity in vivo,respectively.2nd,the composition was determined by the synergistic effects of Rhodiolarosea,Cordycepsmilitaris,and Rhubarb root on insulin resistance in DIO mice.3rd,in accordance with the IC50 values from the L9(34)and L8(27)orthogonal experiments targeting on PTP1 B and α-glucosidase,respectively,the best rate of Rhodiolarosea∶Cordycepsmilitaris∶Rhubarbroot was selected.4th,the effective dose for the treatment of metabolic syndrome was assessed based on the effects on IGT,hypercholesterolemia,obesity,insulin resistance,respectively,in DIO mice.CONCLUSION The prescription compatibility of Rhodiolarosea,Cordycepsmilitaris,and Rhubarbroot with the rate of 20∶1∶1was formed to treat the metabolic syndrome,such as IGT,hypercholesterolemia,and obesity,with the effective dose of 200 mg·kg-1 by the major mechanism of the improvement on insulin sensitivity.Monarch drug,Rhodiola,can clear away the lung-heat,tonify Qi,resolve stasis and nourish the heart.Adjuvant drug,Cordycepsmilitaris,can tonify the lung Qi and the kidney essence,strong waist and knee,compatibility with Rhodiola to enhance the function of invigorating lung and kidney.Assistant drug,Rhubarb,can clear heat,detoxify,remove blood stasis.These three herbal are compatible to show the effects of tonifying Qi,nourishing essence,cleaning heat,reducing phlegm and resolving masses for the treatment of metabolic syndrome.展开更多
Objective:To describe the prescribing behavior of the physician in Kerala,and to describe types and number of medicine prescribed by doctors so as to rationalize the prescription writing habits of physician.Methods: A...Objective:To describe the prescribing behavior of the physician in Kerala,and to describe types and number of medicine prescribed by doctors so as to rationalize the prescription writing habits of physician.Methods: A six month study was done to define the pattern of drug use in Medical college hospital,Calicut. The data and the other important aspects of study were collected by personal interview,questionnaires meant for doctors and prescription monitoring Performa for the patients issued by WHO. The findings of survey were critically analyzed.Results: The results indicated that majority of doctors prescribed on the basis of patient's history,patient's feed back report,drug availability,brand cost,quality of drug and ADRs. All the interviewed doctors looked upon the side effects. It was found that individual drugs were prescribed more than fixed dose combinations (85.6%). Out of these 71.5% were prescribed as branded and 28.5% as generic. The demographic data,like name,age,sex,disease diagnosis were available on all the patient health care cards and these constituted an essential part of the prescription particularly age and sex being an important in deciding the dose preference for patients. Socio economically most patients were found to be in the grade ⅡI (Population below poverty line). During the study of drug related parameters,doses were mentioned only in 80% of the prescription. But duration of the drug therapy was found to be only 30%.These indicated the irrational use of prescription. Branded drugs prescribed were found to be 71.5%,which showed a trend towards a good prescription habit. The results indicated the rational use of drug.Conclusion: The survey reveales that most of the doctors in Medical college hospital,Calicut are maintaining rational use of drugs and their prescription writing habits was found to be good.展开更多
针对目前植保无人机处方作业任务规划优化方法的空缺,提出了基于改进蛛蜂算法(improved spider wasp optimizer,ISWO)的植保无人机任务规划方法。在预生成的全覆盖作业路径基础上,以电池里程与药箱容量为约束,考虑处方变量施药,以植保...针对目前植保无人机处方作业任务规划优化方法的空缺,提出了基于改进蛛蜂算法(improved spider wasp optimizer,ISWO)的植保无人机任务规划方法。在预生成的全覆盖作业路径基础上,以电池里程与药箱容量为约束,考虑处方变量施药,以植保无人机任务总体时间最短与非作业路程总距离最小为目标建立了任务规划模型,并且采用ISWO算法对模型进行求解。ISWO在蛛蜂算法的基础上融入了学习因子的正余弦自适应收缩策略与贪婪均值思想的种群初始化办法。经算法有效性分析与案例分析,相较于传统的最大作业距离模式,ISWO能够大幅度降低作业总体时间与非作业路程长度。相较于四个启发式算法WOA、GWO、PSO、SWO,ISWO在寻优性能与稳定性上有突出表现,可为植保无人机处方作业规划提供一定的参考。展开更多
目的:探究芪黄固肾通络方对糖尿病肾病(DKD)小鼠的治疗效果,及其通过过氧化酶增殖激活受体γ(PPAR-γ)调控脂代谢和自噬对肾脏纤维化和足细胞的影响。方法:将C57BL6/J小鼠分为正常组、模型组和芪黄固肾通络方低、中、高剂量组,用链脲佐...目的:探究芪黄固肾通络方对糖尿病肾病(DKD)小鼠的治疗效果,及其通过过氧化酶增殖激活受体γ(PPAR-γ)调控脂代谢和自噬对肾脏纤维化和足细胞的影响。方法:将C57BL6/J小鼠分为正常组、模型组和芪黄固肾通络方低、中、高剂量组,用链脲佐菌素(STZ)诱导DKD模型。检测血糖、血肌酐(Scr)、尿素氮(BUN)、24 h尿蛋白(24 h pro)等指标,观察肾脏超微结构和病理变化,检测PPAR-γ、微管相关蛋白1A/1B-轻链3(LC-3)、p62蛋白(SQSTM-1/p62)等蛋白表达。体外培养小鼠肾足细胞(MPC-5),用含药血清干预,检测相关蛋白表达和脂质积累。结果:模型组小鼠表现出明显的肾脏损伤,足突蛋白(Podocin)、肾病蛋白(Nephrin)表达下降,血糖、尿蛋白、Scr、BUN上升,PPAR-γ、p62等蛋白表达增加,LC3、苄氯素1(Beclin-1)下降,脂质沉积增多。芪黄固肾通络方能显著改善上述指标,降低PPAR-γ、p62表达,增加LC-3、Beclin-1表达,减少脂质沉积。体外实验显示,芪黄固肾通络方含药血清上调Beclin-1、LC3表达,下调p62、PPAR-γ表达,减轻脂质积累。结论:芪黄固肾通络方可能通过下调PPAR-γ,增强自噬,减轻DKD小鼠肾脏纤维化,保护足细胞,对DKD具有治疗作用。展开更多
目的系统评价中医经典名方及中成药治疗肺纤维化的临床疗效与安全性,为中医药在该病治疗中的应用提供循证医学依据。方法检索CNKI、WanFang、VIP、PubMed、Web of Science数据库自建库至2023年4月以来公开发表的经典名方和中成药治疗肺...目的系统评价中医经典名方及中成药治疗肺纤维化的临床疗效与安全性,为中医药在该病治疗中的应用提供循证医学依据。方法检索CNKI、WanFang、VIP、PubMed、Web of Science数据库自建库至2023年4月以来公开发表的经典名方和中成药治疗肺纤维化的随机对照试验(RCTs),采用Cochrane偏倚风险工具评估文献质量,使用RevMan 5.4进行Meta分析。结果纳入35篇RCTs(共计2440例病例)。Meta分析显示,经典名方及中成药联合西药治疗较单纯西药治疗:①提高临床总有效率(RR=1.28,95%CI[1.21,1.35],P<0.00001);②改善患者用力肺活量(L:SMD=1.47,95%CI[0.79,2.16],P<0.00001),(%:SMD=0.92,95%CI[0.45,1.40],P<0.0001)、一氧化碳弥散量(mL:SMD=0.95,95%CI[0.69,1.20],P<0.00001),(%:SMD=0.44,95%CI[0.15,0.74],P=0.003)和动脉血氧分压(SMD=2.39,95%CI[1.76,3.02],P<0.00001);③未增加不良反应发生率(RR=0.93,95%CI[0.60,1.44],P=0.75)。结论中西药联合治疗肺纤维化在提高临床总有效率、改善肺功能及动脉血氧分压方面优于单纯西药治疗,且安全性良好。未来需开展更多大样本、高质量RCTs,进一步提供科学严谨的循证医学证据。展开更多
基金The project supported by National Major Special Project on New Drug Innovation of China(2009ZX09103-4322012ZX09103-101-063+1 种基金2012ZX09301002-004)We also appreciate the support of Novo Nordisk Union Diabetes Research Talent Fund
文摘OBJECTIVE To investigate the compatibility of the prescription Rhodiolarosea,Cordycepsmilitaris,and Rhubarb root.METHODS The DIO mice accompanying with insulin resistance,hypercholesterolemia and obesity were induced by high fat diet(HFD)in C57BL/6mice.For evaluating the insulin sensitivity in vivo,the values of serum insulin,HOMA-IR,and the glucose infuse rate(GIR)in hyperinsulinemiceuglycemic clamp test were determined;the time-dependent blood glucose changes were measured after insulin loading in insulin tolerance test(ITT),or after glucose loading in glucose tolerance test(GTT),respectively.Recombinant human protein tyrosine phosphatase 1B(PTP1B)was overexpressed by hGST-PTP1BBL21 E.coli and purified by GST affinity chromatography.The activities of PTP1 Band glutamine:fructose 6-phosphate amidotransferase(GFAT)were measured by the pNPP and GDH method,respectively.The activity ofα-glucosidase was determined with the substrates of p-nitrophenyl-alpha-D-glucopyranoside(pNPG).The IC50 value was calculated using the software GraphPad Prism 5.RESULTS 1st,the standard for the raw materials and for the extraction process of Rhodiolarosea,Cordycepsmilitaris,and Rhubarbroot are formulated according to the inhibition on PTP1 B,α-glucosidase,and GFAT in vitro,and the improvement on impaired glucose tolerance(IGT),hypercholesterolemia,and obesity in vivo,respectively.2nd,the composition was determined by the synergistic effects of Rhodiolarosea,Cordycepsmilitaris,and Rhubarb root on insulin resistance in DIO mice.3rd,in accordance with the IC50 values from the L9(34)and L8(27)orthogonal experiments targeting on PTP1 B and α-glucosidase,respectively,the best rate of Rhodiolarosea∶Cordycepsmilitaris∶Rhubarbroot was selected.4th,the effective dose for the treatment of metabolic syndrome was assessed based on the effects on IGT,hypercholesterolemia,obesity,insulin resistance,respectively,in DIO mice.CONCLUSION The prescription compatibility of Rhodiolarosea,Cordycepsmilitaris,and Rhubarbroot with the rate of 20∶1∶1was formed to treat the metabolic syndrome,such as IGT,hypercholesterolemia,and obesity,with the effective dose of 200 mg·kg-1 by the major mechanism of the improvement on insulin sensitivity.Monarch drug,Rhodiola,can clear away the lung-heat,tonify Qi,resolve stasis and nourish the heart.Adjuvant drug,Cordycepsmilitaris,can tonify the lung Qi and the kidney essence,strong waist and knee,compatibility with Rhodiola to enhance the function of invigorating lung and kidney.Assistant drug,Rhubarb,can clear heat,detoxify,remove blood stasis.These three herbal are compatible to show the effects of tonifying Qi,nourishing essence,cleaning heat,reducing phlegm and resolving masses for the treatment of metabolic syndrome.
文摘Objective:To describe the prescribing behavior of the physician in Kerala,and to describe types and number of medicine prescribed by doctors so as to rationalize the prescription writing habits of physician.Methods: A six month study was done to define the pattern of drug use in Medical college hospital,Calicut. The data and the other important aspects of study were collected by personal interview,questionnaires meant for doctors and prescription monitoring Performa for the patients issued by WHO. The findings of survey were critically analyzed.Results: The results indicated that majority of doctors prescribed on the basis of patient's history,patient's feed back report,drug availability,brand cost,quality of drug and ADRs. All the interviewed doctors looked upon the side effects. It was found that individual drugs were prescribed more than fixed dose combinations (85.6%). Out of these 71.5% were prescribed as branded and 28.5% as generic. The demographic data,like name,age,sex,disease diagnosis were available on all the patient health care cards and these constituted an essential part of the prescription particularly age and sex being an important in deciding the dose preference for patients. Socio economically most patients were found to be in the grade ⅡI (Population below poverty line). During the study of drug related parameters,doses were mentioned only in 80% of the prescription. But duration of the drug therapy was found to be only 30%.These indicated the irrational use of prescription. Branded drugs prescribed were found to be 71.5%,which showed a trend towards a good prescription habit. The results indicated the rational use of drug.Conclusion: The survey reveales that most of the doctors in Medical college hospital,Calicut are maintaining rational use of drugs and their prescription writing habits was found to be good.
文摘针对目前植保无人机处方作业任务规划优化方法的空缺,提出了基于改进蛛蜂算法(improved spider wasp optimizer,ISWO)的植保无人机任务规划方法。在预生成的全覆盖作业路径基础上,以电池里程与药箱容量为约束,考虑处方变量施药,以植保无人机任务总体时间最短与非作业路程总距离最小为目标建立了任务规划模型,并且采用ISWO算法对模型进行求解。ISWO在蛛蜂算法的基础上融入了学习因子的正余弦自适应收缩策略与贪婪均值思想的种群初始化办法。经算法有效性分析与案例分析,相较于传统的最大作业距离模式,ISWO能够大幅度降低作业总体时间与非作业路程长度。相较于四个启发式算法WOA、GWO、PSO、SWO,ISWO在寻优性能与稳定性上有突出表现,可为植保无人机处方作业规划提供一定的参考。
文摘目的:探究芪黄固肾通络方对糖尿病肾病(DKD)小鼠的治疗效果,及其通过过氧化酶增殖激活受体γ(PPAR-γ)调控脂代谢和自噬对肾脏纤维化和足细胞的影响。方法:将C57BL6/J小鼠分为正常组、模型组和芪黄固肾通络方低、中、高剂量组,用链脲佐菌素(STZ)诱导DKD模型。检测血糖、血肌酐(Scr)、尿素氮(BUN)、24 h尿蛋白(24 h pro)等指标,观察肾脏超微结构和病理变化,检测PPAR-γ、微管相关蛋白1A/1B-轻链3(LC-3)、p62蛋白(SQSTM-1/p62)等蛋白表达。体外培养小鼠肾足细胞(MPC-5),用含药血清干预,检测相关蛋白表达和脂质积累。结果:模型组小鼠表现出明显的肾脏损伤,足突蛋白(Podocin)、肾病蛋白(Nephrin)表达下降,血糖、尿蛋白、Scr、BUN上升,PPAR-γ、p62等蛋白表达增加,LC3、苄氯素1(Beclin-1)下降,脂质沉积增多。芪黄固肾通络方能显著改善上述指标,降低PPAR-γ、p62表达,增加LC-3、Beclin-1表达,减少脂质沉积。体外实验显示,芪黄固肾通络方含药血清上调Beclin-1、LC3表达,下调p62、PPAR-γ表达,减轻脂质积累。结论:芪黄固肾通络方可能通过下调PPAR-γ,增强自噬,减轻DKD小鼠肾脏纤维化,保护足细胞,对DKD具有治疗作用。
文摘目的系统评价中医经典名方及中成药治疗肺纤维化的临床疗效与安全性,为中医药在该病治疗中的应用提供循证医学依据。方法检索CNKI、WanFang、VIP、PubMed、Web of Science数据库自建库至2023年4月以来公开发表的经典名方和中成药治疗肺纤维化的随机对照试验(RCTs),采用Cochrane偏倚风险工具评估文献质量,使用RevMan 5.4进行Meta分析。结果纳入35篇RCTs(共计2440例病例)。Meta分析显示,经典名方及中成药联合西药治疗较单纯西药治疗:①提高临床总有效率(RR=1.28,95%CI[1.21,1.35],P<0.00001);②改善患者用力肺活量(L:SMD=1.47,95%CI[0.79,2.16],P<0.00001),(%:SMD=0.92,95%CI[0.45,1.40],P<0.0001)、一氧化碳弥散量(mL:SMD=0.95,95%CI[0.69,1.20],P<0.00001),(%:SMD=0.44,95%CI[0.15,0.74],P=0.003)和动脉血氧分压(SMD=2.39,95%CI[1.76,3.02],P<0.00001);③未增加不良反应发生率(RR=0.93,95%CI[0.60,1.44],P=0.75)。结论中西药联合治疗肺纤维化在提高临床总有效率、改善肺功能及动脉血氧分压方面优于单纯西药治疗,且安全性良好。未来需开展更多大样本、高质量RCTs,进一步提供科学严谨的循证医学证据。
