Components with strong adsorption capacity for cholates from buckwheat proteins were screened, separated and purified by several methods, and the effects of ultra-high-pressure(UHP) on the structure and function of bu...Components with strong adsorption capacity for cholates from buckwheat proteins were screened, separated and purified by several methods, and the effects of ultra-high-pressure(UHP) on the structure and function of buckwheat 13S globulin(BW13SG) were studied. Samples were treated by UHP at different p H(3.0 and 7.0) value(s) and at 100–500 MPa for 10–30 min. The results showed that the tertiary structure of BW13SG was partially denatured and aggregated. The decrease in the unordered structure indicated that UHP resulted in a looser secondary structure of BW13SG. UHP treatment also increased solubility, emulsion activity and stability, foaming capacity and stability. The samples treated at 500 MPa, p H 3.0 for 30 min had the most enhanced functionality. Moreover, under this condition, the sodium cholate and sodium deoxycholate adsorption capacities of BW13SG were both higher than 98% and the adsorption capacity of sodium taurocholate, which can be difficult to adsorb, was higher than 60%.展开更多
Wheat embryo globulin(WEG)has been proven to possess multiple biological activities,including antioxidative properties,immunomodulatory,and so on.Aged mouse model were established by subcutaneous injection of D-galact...Wheat embryo globulin(WEG)has been proven to possess multiple biological activities,including antioxidative properties,immunomodulatory,and so on.Aged mouse model were established by subcutaneous injection of D-galactose(D-gal),and the effects of WEG on learning,memory,and antioxidant capacity in aging mice were explored through behavioural tests and antioxidant enzyme activities determination.Compared with the Model group,WEG improved the percentage of the platform quadrant,increased the number of crossing platforms,and enhanced the identification indexs.WEG also increased total antioxidant capacity(T-AOC),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)activities in the liver and brains of aging mice,and reduced malondialdehyde(MDA)content.Pathological observations indicated that WEG protected against damage to brain in D-gal-induced aging mice.These results effectively revealed that WEG not only improved the abilities of learning and memory,and the cognitive impairment,but also delayed the aging process of the D-gal-induced mice.展开更多
目的:探讨关节假体周围感染(periprosthetic joint infection,PJI)的新型血液学标志物在术前诊断PJI的应用价值。方法:单中心回顾性分析2016年1月至2022年6月收治的全关节置换术后需全髋或全膝关节翻修的患者149例,男63例,女86例;年龄47...目的:探讨关节假体周围感染(periprosthetic joint infection,PJI)的新型血液学标志物在术前诊断PJI的应用价值。方法:单中心回顾性分析2016年1月至2022年6月收治的全关节置换术后需全髋或全膝关节翻修的患者149例,男63例,女86例;年龄47~93(69.5±11.8)岁。诊断为PJI的患者46例(PJI组),男22例,女24例;年龄(71.3±12.5)岁;身体质量指数(body mass index,BMI)为(26.4±3.1)kg·m^(-2)。诊断为无菌性假体松动的患者103例(无菌性组),男41例,女62例;年龄(68.7±11.4)岁;BMI为(25.8±3.5)kg·m^(-2)。入院时测定C-反应蛋白、红细胞沉降率(erythrocyte sedimentation rate,ESR)、白蛋白、球蛋白、白球比、D-二聚体和纤维蛋白原。通过受试者工作特征(receiver operating characteristic,ROC)曲线、敏感度和特异性分析比较各血液标志物对术前诊断PJI的价值。结果:PJI组的C-反应蛋白16.6(7.6,4.5)mg·L^(-1)、ESR为17.0(12.8,35.5)mm·h^(-1)、D-二聚体1.0(0.5,3.1)μg·L^(-1)、纤维蛋白原4.2(3.2,5.5)g·L^(-1)、球蛋白35.4(31.0,38.9)g·L^(-1)均高于无菌性组4.2(2.6,7.8)mg·L^(-1),12.0(8.0,20.0)mm·h^(-1),0.4(0.2,0.7)μg·L^(-1),2.8(2.4,3.3)g·L^(-1),28.6(24.5,31.9)g·L^(-1)(P<0.05);相反,PJI组的白蛋白35.3(32.3,37.5)g·L^(-1)和白球比1.0(0.9,1.1)均明显低于无菌性组39.8(36.1,41.8)g·L^(-1),1.4(1.3,1.5),P<0.05。相比于其他血液学标志物,只有白球比和纤维蛋白原的曲线下面积(area under the curve,AUC)>0.8。其中纤维蛋白原的最佳截断值为3.4 g·L^(-1),AUC为0.820,敏感度为69.57%,特异性为84.47%。白球比的最佳截断值为1.18,AUC为0.813,敏感度为82.61%,特异性为78.64%。结论:白球比和纤维蛋白原是术前诊断PJI可靠的血液学标志物。展开更多
基金the financial support by the Open Project Program of State Key Laboratory of Dairy Biotechnology (No. SKLDB2018-002)National Natural Science Foundation of China (No. 31871805 & No. 31501437)+1 种基金Shanghai Municipal Education Commission (Plateau Discipline Construction Program)China Agriculture Research System (CARS-08-D2)。
文摘Components with strong adsorption capacity for cholates from buckwheat proteins were screened, separated and purified by several methods, and the effects of ultra-high-pressure(UHP) on the structure and function of buckwheat 13S globulin(BW13SG) were studied. Samples were treated by UHP at different p H(3.0 and 7.0) value(s) and at 100–500 MPa for 10–30 min. The results showed that the tertiary structure of BW13SG was partially denatured and aggregated. The decrease in the unordered structure indicated that UHP resulted in a looser secondary structure of BW13SG. UHP treatment also increased solubility, emulsion activity and stability, foaming capacity and stability. The samples treated at 500 MPa, p H 3.0 for 30 min had the most enhanced functionality. Moreover, under this condition, the sodium cholate and sodium deoxycholate adsorption capacities of BW13SG were both higher than 98% and the adsorption capacity of sodium taurocholate, which can be difficult to adsorb, was higher than 60%.
基金funded by Zhongyuan Scholars in Henan Province(192101510004)Major Science and Technology Projects for Public Welfare of Henan Province(201300110300)+2 种基金Innovation Demonstration Special Project of Henan Province(201111110100)financially supported by Key Project Foundation of Natural Science Research of Universities of Henan Province in China(20A550004)Fundamental Research Funds for the Henan Provincial Colleges and Universities in Henan University of Technology(HAUT)and High-Level Talents Research Fund of HAUT(2018QNJH13,and 2018BS012)。
文摘Wheat embryo globulin(WEG)has been proven to possess multiple biological activities,including antioxidative properties,immunomodulatory,and so on.Aged mouse model were established by subcutaneous injection of D-galactose(D-gal),and the effects of WEG on learning,memory,and antioxidant capacity in aging mice were explored through behavioural tests and antioxidant enzyme activities determination.Compared with the Model group,WEG improved the percentage of the platform quadrant,increased the number of crossing platforms,and enhanced the identification indexs.WEG also increased total antioxidant capacity(T-AOC),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)activities in the liver and brains of aging mice,and reduced malondialdehyde(MDA)content.Pathological observations indicated that WEG protected against damage to brain in D-gal-induced aging mice.These results effectively revealed that WEG not only improved the abilities of learning and memory,and the cognitive impairment,but also delayed the aging process of the D-gal-induced mice.
文摘目的:探讨关节假体周围感染(periprosthetic joint infection,PJI)的新型血液学标志物在术前诊断PJI的应用价值。方法:单中心回顾性分析2016年1月至2022年6月收治的全关节置换术后需全髋或全膝关节翻修的患者149例,男63例,女86例;年龄47~93(69.5±11.8)岁。诊断为PJI的患者46例(PJI组),男22例,女24例;年龄(71.3±12.5)岁;身体质量指数(body mass index,BMI)为(26.4±3.1)kg·m^(-2)。诊断为无菌性假体松动的患者103例(无菌性组),男41例,女62例;年龄(68.7±11.4)岁;BMI为(25.8±3.5)kg·m^(-2)。入院时测定C-反应蛋白、红细胞沉降率(erythrocyte sedimentation rate,ESR)、白蛋白、球蛋白、白球比、D-二聚体和纤维蛋白原。通过受试者工作特征(receiver operating characteristic,ROC)曲线、敏感度和特异性分析比较各血液标志物对术前诊断PJI的价值。结果:PJI组的C-反应蛋白16.6(7.6,4.5)mg·L^(-1)、ESR为17.0(12.8,35.5)mm·h^(-1)、D-二聚体1.0(0.5,3.1)μg·L^(-1)、纤维蛋白原4.2(3.2,5.5)g·L^(-1)、球蛋白35.4(31.0,38.9)g·L^(-1)均高于无菌性组4.2(2.6,7.8)mg·L^(-1),12.0(8.0,20.0)mm·h^(-1),0.4(0.2,0.7)μg·L^(-1),2.8(2.4,3.3)g·L^(-1),28.6(24.5,31.9)g·L^(-1)(P<0.05);相反,PJI组的白蛋白35.3(32.3,37.5)g·L^(-1)和白球比1.0(0.9,1.1)均明显低于无菌性组39.8(36.1,41.8)g·L^(-1),1.4(1.3,1.5),P<0.05。相比于其他血液学标志物,只有白球比和纤维蛋白原的曲线下面积(area under the curve,AUC)>0.8。其中纤维蛋白原的最佳截断值为3.4 g·L^(-1),AUC为0.820,敏感度为69.57%,特异性为84.47%。白球比的最佳截断值为1.18,AUC为0.813,敏感度为82.61%,特异性为78.64%。结论:白球比和纤维蛋白原是术前诊断PJI可靠的血液学标志物。
