Humic acids can promote the germination of many vegetable seeds,but the key active components remain unclear.This study utilized nutrient content,cross polarization magic angle spin ^(13)C solid magnetic resonance(CPM...Humic acids can promote the germination of many vegetable seeds,but the key active components remain unclear.This study utilized nutrient content,cross polarization magic angle spin ^(13)C solid magnetic resonance(CPMAS-^(13)C-NMR)and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)to characterize the chemical components of humic acids.Tomato seed germination index(GI)was determined with the goal of screening the key active components of humic acids.Humic acids had a significantly higher nutrient content,except for the total nitrogen(TN)and the total phosphorus(TP)contents.Humic acids had a higher content of O-CH_(3)/NCH,aromatic C-O and carbonyl C compared to weathered coal,with significantly lower anomeric C,aromatic C and O-alkyl C/alkyl C.There were 611 different compounds identified among the test materials using UHPLC-MS.Humic acids also had a significantly higher GI(158.0%and 153.1%)than weathered coal(85.5%).The organic matter(OM),TP and available potassium(AK)contents in humic acids were significantly positively correlated with GI,and available phosphorus(AP)was significantly negatively correlated.Among the carbon components,O-CH3/NCH,aromatic C-O and O-alkyl C/alkyl C were significantly positively correlated with GI,while anomeric C was significantly negatively correlated.Furthermore,among the top 10 positive and five negative correlation compounds,lipids and lipid-like molecules[armexifolin,boviquinone 4,3-methyladipic acid,lxocarpalactone A,monic acid,DG(20:1(11Z)/18:4(6Z,9Z,12Z,15Z)/0:0),and brassinolide]and organic acids and derivatives(N-acetylglutamic acid,8-hydroxy-5,6-octadienoic acid,acetyl-L-tyrosine,and hydroxyprolyl-methionine)in humic acids might be crucial active components for improving tomato seed germination.The results provided direct evidence for the identification of bioactive molecules of humic acids,and a scientific basis for the precise utilization of bioactive molecular components of humic acids in sustainable agricultural development.展开更多
To analyze the effect of wood vinegar on seed germination and seedling growth, the seeds of corn are dipped in wood vinegar of different densities. The results showed that significant effects were found through all th...To analyze the effect of wood vinegar on seed germination and seedling growth, the seeds of corn are dipped in wood vinegar of different densities. The results showed that significant effects were found through all the treatments on the seed germination rate, the seed germinating energy and the germinating index. The regress CUBICS curves were developed to describe the relation. The reasonable parameters range was obtained. At the same time, the corn was cultivated by wood vinegar of different densities, aiming to study the effect of wood vinegar on biomass. It showed that all treatments had obvious effects on the seedling length and dry weight aboveground, the chlorophyll and dry weight underground were not included. The research results could be used to direct the wood vinegar deeply refining process and product development.展开更多
To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on ger...To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on germination status, amylase activity and isoenzyme were studied in this paper. The results showed that germination status including germination energy（GE）, germination rate（GR）, relative germination energy（RGE） and relative germination rate（RGR） significantly decreased as the same as the activities of alpha-amylase, beta-amylase and the total amylase under salt stress. Soaking with exogenous proline improved the germination status of rice under salt stress. Moreover, GE and RGE of salt-stressed rice were improved with increasing of proline concentration at the range of 5-45 mmol ·L-1. Soaking with 15 mmol ·L-1 and 30 mmol ·L-1 proline significantly improved the amylase activities（e.g. alpha-amylase, beta-amylase and total amylase） of rice under salt stress. Salt stress inhibited the express of beta-amylase isoenzyme temporarily, but had few impacts on alpha-amylase isozyme. Soaking with 30 mmol ·L-1 proline brightened District I and increased the width of ＇i＇ brand in District II of alpha-amylase isoenzyme, but had few impacts on beta-amylase isoenzyme. In a word, soaking with proline could effectively alleviate the inhibitory effects of salt stress on seed germination.展开更多
Background:Cottonseed oil and protein content as well as germination traits are major indicators of seed quality.However,the responses of these indicators to plant density and mepiquat chloride(MC)are still uncertain....Background:Cottonseed oil and protein content as well as germination traits are major indicators of seed quality.However,the responses of these indicators to plant density and mepiquat chloride(MC)are still uncertain.To investigate plant density and MC effects on cotto nseed yield and main quality parameters,we con ducted a twoyear field experiment including four plant densities(1.35,2.55,3.75 and 4.95 plants·m^-2)and two closes of MC(0 and 135g·hm^-2)in Dafeng,Jiangsu Province,in 2013 and 2014.Results:The application of MC reduced plant height,fruit branch length and fruiting branch number under different plant densities,resulting in a lower and more compact plant canopy.Cottonseed yield showed a nonlinear increase as plant density increasing and achieved the highest value at 3.75 plants·m^-2,regardless of MC application.No significant interactio ns were found between plant density and MC for cotton seed yield and quality parameters.The 100-seed weight,cottonseed oil content and vigor index significantly decreased as plant density increased,while these parameters significantly increased with MC applying under different plant densities.Seed vigor index was positively correlated with 100-seed weight and seed oil con tent across different plant densities and MC treatments.Conclusions:Thus,application of MC could realize a win-win situation between cottonseed yield and main quality parameters under various densities;and plant density of 3.75 plants·m^-2 combined with 135 g·hm^-2 of MC applying is optimal for high cottonseed yield and quality in this cotton production area.展开更多
Convolvulus arvensis is a toxic allelopathic weed that suppresses germination and growth of crops. The prime object of present study was to investigate effect of Convolvulus arvensis water extract on germination and p...Convolvulus arvensis is a toxic allelopathic weed that suppresses germination and growth of crops. The prime object of present study was to investigate effect of Convolvulus arvensis water extract on germination and performance of okra with different seed sizes. The seeds of okra variety pusa green were separated into three different sizes, viz large size （4.00-5.00 mm）, medium size （3.00-3.50 mm） and small size （2.00-3.50 mm）, and then soaked in allelopathic plant bindweed water extract and kept in patrisdishes for germination into the germinator at 15℃. The experiment was laid out using Complete Randomized Design （CRD） with three replications. The results showed that after sowing of 12 days the highest germination was observed in non-treated seeds as compared to seeds treated in Convolvulus arvensis water extract for 1 h, further non-treated large seeds produced maximum plants as compared to treated small seeds after sowing of 24 days. Meanwhile, root length, shoot length, root fresh and dry weight, shoot fresh and dry weight were recorded higher in non-treated large seeds as compared to small seeds soaked for 1 h in Convolvulus arvensis allelopthic water extract. It could be found that Convolvulus arvensis affected germination, seed growth and overall performance of okra, further presence Convolvulus arvensis in crops could cause negative impact on germination and integrity of okra crops.展开更多
Bioassay method was used to study the biological activity of different polar solvent extracts of False ragweed seed. Specially, water extracts of False ragweed has an effect on plant germination and root length. The s...Bioassay method was used to study the biological activity of different polar solvent extracts of False ragweed seed. Specially, water extracts of False ragweed has an effect on plant germination and root length. The study found that False ragweed seed extracts had different degrees of inhibition on many plant seed germinations, and inhibited the germination of cucumber seeds best, the maximum inhibition rate was 79.5%, for cabbage, sorghum and maize seed germination inhibition rate was more than 30%. False ragweed seed extracts also had strong inhibition on cucumber and many other plant root length, and inhibited the root length of cucumber best followed by red beans. The result showed that False ragweed seed extracts contained some materials that could inhibit germination and growth of some plants.展开更多
To study the effect of soil water and salt environment factors on the root growth of cotton under different moisture control,three different emergence water volumes(60,105,and 150 m^(3)/hm^(2)),two different frequenci...To study the effect of soil water and salt environment factors on the root growth of cotton under different moisture control,three different emergence water volumes(60,105,and 150 m^(3)/hm^(2)),two different frequencies(high frequency and low frequency)and one double film cover winter irrigation control treatment(CK:2250 m^(3)/hm^(2))were set up to analyze the spatial distribution patterns of soil water and salt environment and root density in dry sown and wet emerged cotton fields under diffe-rent moisture control conditions.The results show that the soil water content and water infiltration range gradually become larger with the increase of seedling water quantity,and the larger the seedling water quantity,the higher the soil water content.With the same seedling water quantity,the soil water content of the high-frequency(HF)treatment becomes obviously larger.The soil conductivity of each treatment tends to decrease gradually with the increase of seedling water and drip frequency,among which the distribution of soil conductivity of S6 treatment is closest to that of CK.With the increase in soil depth,the soil conductivity tends to increase first and then decrease.Compared with the low-frequency(LF)treatment,the high-frequency treatment shows a significantly deeper soil salt accumulation layer.The root length density(RLD)of cotton gradually increases with the amount of seedling water and the frequency of dripping.The soil layer of root distribution gradually deepens with the amount of seedling water in the vertical direction,and the RLD value in the horizontal direction is significantly greater in the mulched area than that in the bare area between films.This research can serve as a solid scientific foundation for the use of dry sowing and wet emergence techniques in cotton fields in southern Xinjiang.展开更多
Marx’s spirit of pursuing truth,his hatred of exploitation system and sympathy for laborers prompted him to move from the young Hegelian school to fehbaism and eventually to communism,and completed the transformation...Marx’s spirit of pursuing truth,his hatred of exploitation system and sympathy for laborers prompted him to move from the young Hegelian school to fehbaism and eventually to communism,and completed the transformation from idealism to thorough dialectical materialism in the world view.To do so,he constructed his own theoretical system based on criticism and targeted the social practice,providing ideological weapon for the transformation of unreasonable social forms.Thereby,the unit of"critical weapon"and"weapon of criticism"was accomplished historically and logically,fulfilling the construction of his critical view.展开更多
In order to enhance the seed storability and supply high-quality seeds for vegetation restoration in the arid and semi-arid regions in Northwest China,the effects of ultra-drying and accelerated aging on the physiolog...In order to enhance the seed storability and supply high-quality seeds for vegetation restoration in the arid and semi-arid regions in Northwest China,the effects of ultra-drying and accelerated aging on the physiological characterstics of Melilotus suaveolens seeds were studied.Melilotus suaveolens seeds were dried in a desiccator containing silica gel to 80,53,42,33,23 and 16 g·kg-1 of moisture contents（MC）,respectively.The parameters of the seed quality including germination energy（GE）,germination percentage（GP）,relative conductivity（RC）,dehydrogenase activity（DA） and α-amylase activity（AA） were determined after ultra-drying and accelerated aging.The results showed that ultra-dried seeds with 42 g·kg-1 of MC showed the minimum changes of GE and GP before and after seed aging.Moreover,ultra-dry seeds with 42 g·kg-1 of MC showed higher DA and AA,and lower RC than non-ultra-drying seeds.Therefore,ultra-drying to 42 g·kg-1 of MC was helpful for M.suaveolens seed storage.展开更多
The metabolism of fats, proteins and carbohydrates and the change of enzyme activity in sceds of Sophora japonica during germination were studied by methods of Soxhlet's extraction, flow injection and colorimctric...The metabolism of fats, proteins and carbohydrates and the change of enzyme activity in sceds of Sophora japonica during germination were studied by methods of Soxhlet's extraction, flow injection and colorimctric analysis of speetrophotometer for providing theoretical basis for germination and storage of seeds of forest trees. The results arc as follows:(1)The activity of enzymes in the seeds does not always coincide with the increase or deercase of their corresponding substances during germination of the seeds of Sophora japonica.(2)Proteins were first utilized during germination of the seeds.(3)The fatty acids reducing sugar and amino acids in the radiele+plumular axis were used pseedcntly over all others when the embryos grew.(4)excessive small-molecular substances produced by hydrolysis would accumulate in tissres and sometimes they were stored temporarily in the form of storage substances and finally used for embryo growth.展开更多
Factors affecting the in vitro germination and growth of Taxus cuspidata embryos were examined. DCR medium was the best among 6 basal media tested; embryos from stage II seeds were the optimal developmental stage for ...Factors affecting the in vitro germination and growth of Taxus cuspidata embryos were examined. DCR medium was the best among 6 basal media tested; embryos from stage II seeds were the optimal developmental stage for in vitro germination; as the seeds approached maturity, germinability of embryos decrease. Embryos cultured in darkness tended to form callus and decrease the frequency of germination; inoculation method obviously affected frequency of embryo germination. Embryos cultured in optimal conditions developed into seedlings in 2 months.展开更多
基金Supported by the National Natural Science Foundation of China(42207371)the Technological Project of Jiangsu Vocational College of Agriculture and Forestry(2021kj17)+1 种基金Yafu Technology Innovation and Service Major Project of Jiangsu Vocational College of Agriculture and Forestry(2024kj01)Key Research Projects of Jiangsu Vocational College of Agriculture and Forestry(2023kj14)。
文摘Humic acids can promote the germination of many vegetable seeds,but the key active components remain unclear.This study utilized nutrient content,cross polarization magic angle spin ^(13)C solid magnetic resonance(CPMAS-^(13)C-NMR)and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)to characterize the chemical components of humic acids.Tomato seed germination index(GI)was determined with the goal of screening the key active components of humic acids.Humic acids had a significantly higher nutrient content,except for the total nitrogen(TN)and the total phosphorus(TP)contents.Humic acids had a higher content of O-CH_(3)/NCH,aromatic C-O and carbonyl C compared to weathered coal,with significantly lower anomeric C,aromatic C and O-alkyl C/alkyl C.There were 611 different compounds identified among the test materials using UHPLC-MS.Humic acids also had a significantly higher GI(158.0%and 153.1%)than weathered coal(85.5%).The organic matter(OM),TP and available potassium(AK)contents in humic acids were significantly positively correlated with GI,and available phosphorus(AP)was significantly negatively correlated.Among the carbon components,O-CH3/NCH,aromatic C-O and O-alkyl C/alkyl C were significantly positively correlated with GI,while anomeric C was significantly negatively correlated.Furthermore,among the top 10 positive and five negative correlation compounds,lipids and lipid-like molecules[armexifolin,boviquinone 4,3-methyladipic acid,lxocarpalactone A,monic acid,DG(20:1(11Z)/18:4(6Z,9Z,12Z,15Z)/0:0),and brassinolide]and organic acids and derivatives(N-acetylglutamic acid,8-hydroxy-5,6-octadienoic acid,acetyl-L-tyrosine,and hydroxyprolyl-methionine)in humic acids might be crucial active components for improving tomato seed germination.The results provided direct evidence for the identification of bioactive molecules of humic acids,and a scientific basis for the precise utilization of bioactive molecular components of humic acids in sustainable agricultural development.
基金Supported by Xiniang Production And Construction Corps Industrial Research Project(2008GG27)Study on Agricultural residue making wood vinegar Pyrolysis Process and DeviceGuangdong Nature Science Project:A new type of clean and effi cient agriculture and forestry biomass pyrolysis conversion technology research
文摘To analyze the effect of wood vinegar on seed germination and seedling growth, the seeds of corn are dipped in wood vinegar of different densities. The results showed that significant effects were found through all the treatments on the seed germination rate, the seed germinating energy and the germinating index. The regress CUBICS curves were developed to describe the relation. The reasonable parameters range was obtained. At the same time, the corn was cultivated by wood vinegar of different densities, aiming to study the effect of wood vinegar on biomass. It showed that all treatments had obvious effects on the seedling length and dry weight aboveground, the chlorophyll and dry weight underground were not included. The research results could be used to direct the wood vinegar deeply refining process and product development.
基金Supported by the Project of Twelfth Five-year Plan for Sci & Tech Research of China in Rural Areas(2011BAD35B02-01)Program of Sci & Tech Research of China(2011BAD16B11)
文摘To explore the germination mechanism of salt-stressed rice improved by exogenous proline, and provide a theoretical basis to rice direct sowing technology for salinized soil, the effects of soaking with proline on germination status, amylase activity and isoenzyme were studied in this paper. The results showed that germination status including germination energy（GE）, germination rate（GR）, relative germination energy（RGE） and relative germination rate（RGR） significantly decreased as the same as the activities of alpha-amylase, beta-amylase and the total amylase under salt stress. Soaking with exogenous proline improved the germination status of rice under salt stress. Moreover, GE and RGE of salt-stressed rice were improved with increasing of proline concentration at the range of 5-45 mmol ·L-1. Soaking with 15 mmol ·L-1 and 30 mmol ·L-1 proline significantly improved the amylase activities（e.g. alpha-amylase, beta-amylase and total amylase） of rice under salt stress. Salt stress inhibited the express of beta-amylase isoenzyme temporarily, but had few impacts on alpha-amylase isozyme. Soaking with 30 mmol ·L-1 proline brightened District I and increased the width of ＇i＇ brand in District II of alpha-amylase isoenzyme, but had few impacts on beta-amylase isoenzyme. In a word, soaking with proline could effectively alleviate the inhibitory effects of salt stress on seed germination.
基金financial support from the Special Fund for Agro-scientific Research in the Public Interest(201503109)the Fundamental Research Funds for the Central Universities(KYYJ201802)+1 种基金Jiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP)Jiangsu Overseas Research and Training Program for University Prominent Young and Middleaged Teachers and President(2016),China
文摘Background:Cottonseed oil and protein content as well as germination traits are major indicators of seed quality.However,the responses of these indicators to plant density and mepiquat chloride(MC)are still uncertain.To investigate plant density and MC effects on cotto nseed yield and main quality parameters,we con ducted a twoyear field experiment including four plant densities(1.35,2.55,3.75 and 4.95 plants·m^-2)and two closes of MC(0 and 135g·hm^-2)in Dafeng,Jiangsu Province,in 2013 and 2014.Results:The application of MC reduced plant height,fruit branch length and fruiting branch number under different plant densities,resulting in a lower and more compact plant canopy.Cottonseed yield showed a nonlinear increase as plant density increasing and achieved the highest value at 3.75 plants·m^-2,regardless of MC application.No significant interactio ns were found between plant density and MC for cotton seed yield and quality parameters.The 100-seed weight,cottonseed oil content and vigor index significantly decreased as plant density increased,while these parameters significantly increased with MC applying under different plant densities.Seed vigor index was positively correlated with 100-seed weight and seed oil con tent across different plant densities and MC treatments.Conclusions:Thus,application of MC could realize a win-win situation between cottonseed yield and main quality parameters under various densities;and plant density of 3.75 plants·m^-2 combined with 135 g·hm^-2 of MC applying is optimal for high cottonseed yield and quality in this cotton production area.
文摘Convolvulus arvensis is a toxic allelopathic weed that suppresses germination and growth of crops. The prime object of present study was to investigate effect of Convolvulus arvensis water extract on germination and performance of okra with different seed sizes. The seeds of okra variety pusa green were separated into three different sizes, viz large size （4.00-5.00 mm）, medium size （3.00-3.50 mm） and small size （2.00-3.50 mm）, and then soaked in allelopathic plant bindweed water extract and kept in patrisdishes for germination into the germinator at 15℃. The experiment was laid out using Complete Randomized Design （CRD） with three replications. The results showed that after sowing of 12 days the highest germination was observed in non-treated seeds as compared to seeds treated in Convolvulus arvensis water extract for 1 h, further non-treated large seeds produced maximum plants as compared to treated small seeds after sowing of 24 days. Meanwhile, root length, shoot length, root fresh and dry weight, shoot fresh and dry weight were recorded higher in non-treated large seeds as compared to small seeds soaked for 1 h in Convolvulus arvensis allelopthic water extract. It could be found that Convolvulus arvensis affected germination, seed growth and overall performance of okra, further presence Convolvulus arvensis in crops could cause negative impact on germination and integrity of okra crops.
基金Supported by Study of Biological,Ecological Environment Security of Herbicide-resistant Genetically Modified Soybeans (LBH-Q05034)Youth Science Foundation of Heilongjiang Province of China (QC2010123)
文摘Bioassay method was used to study the biological activity of different polar solvent extracts of False ragweed seed. Specially, water extracts of False ragweed has an effect on plant germination and root length. The study found that False ragweed seed extracts had different degrees of inhibition on many plant seed germinations, and inhibited the germination of cucumber seeds best, the maximum inhibition rate was 79.5%, for cabbage, sorghum and maize seed germination inhibition rate was more than 30%. False ragweed seed extracts also had strong inhibition on cucumber and many other plant root length, and inhibited the root length of cucumber best followed by red beans. The result showed that False ragweed seed extracts contained some materials that could inhibit germination and growth of some plants.
基金National Key Research and Development Plan(2021YFD1900805)Funded Project of Basic Scientific Research Business of Public Welfare Research Institutes in Autonomous Region(KY2022127)。
文摘To study the effect of soil water and salt environment factors on the root growth of cotton under different moisture control,three different emergence water volumes(60,105,and 150 m^(3)/hm^(2)),two different frequencies(high frequency and low frequency)and one double film cover winter irrigation control treatment(CK:2250 m^(3)/hm^(2))were set up to analyze the spatial distribution patterns of soil water and salt environment and root density in dry sown and wet emerged cotton fields under diffe-rent moisture control conditions.The results show that the soil water content and water infiltration range gradually become larger with the increase of seedling water quantity,and the larger the seedling water quantity,the higher the soil water content.With the same seedling water quantity,the soil water content of the high-frequency(HF)treatment becomes obviously larger.The soil conductivity of each treatment tends to decrease gradually with the increase of seedling water and drip frequency,among which the distribution of soil conductivity of S6 treatment is closest to that of CK.With the increase in soil depth,the soil conductivity tends to increase first and then decrease.Compared with the low-frequency(LF)treatment,the high-frequency treatment shows a significantly deeper soil salt accumulation layer.The root length density(RLD)of cotton gradually increases with the amount of seedling water and the frequency of dripping.The soil layer of root distribution gradually deepens with the amount of seedling water in the vertical direction,and the RLD value in the horizontal direction is significantly greater in the mulched area than that in the bare area between films.This research can serve as a solid scientific foundation for the use of dry sowing and wet emergence techniques in cotton fields in southern Xinjiang.
基金a stage achievement of the project of philosophy and social science research foundation of Jiangsu province——“Research on Innovation of Ideological and Political Work in Colleges and Universities from the Perspective of Cultural Self-confidence”(No.2017SJBFDY115)。
文摘Marx’s spirit of pursuing truth,his hatred of exploitation system and sympathy for laborers prompted him to move from the young Hegelian school to fehbaism and eventually to communism,and completed the transformation from idealism to thorough dialectical materialism in the world view.To do so,he constructed his own theoretical system based on criticism and targeted the social practice,providing ideological weapon for the transformation of unreasonable social forms.Thereby,the unit of"critical weapon"and"weapon of criticism"was accomplished historically and logically,fulfilling the construction of his critical view.
基金Supported by National Key Project of Scientifi c and Technical Supporting Programs of China (2006BAD09B06, 2006BAD03A0308)948 Program of Ministry of Water Resources of China (200207)
文摘In order to enhance the seed storability and supply high-quality seeds for vegetation restoration in the arid and semi-arid regions in Northwest China,the effects of ultra-drying and accelerated aging on the physiological characterstics of Melilotus suaveolens seeds were studied.Melilotus suaveolens seeds were dried in a desiccator containing silica gel to 80,53,42,33,23 and 16 g·kg-1 of moisture contents（MC）,respectively.The parameters of the seed quality including germination energy（GE）,germination percentage（GP）,relative conductivity（RC）,dehydrogenase activity（DA） and α-amylase activity（AA） were determined after ultra-drying and accelerated aging.The results showed that ultra-dried seeds with 42 g·kg-1 of MC showed the minimum changes of GE and GP before and after seed aging.Moreover,ultra-dry seeds with 42 g·kg-1 of MC showed higher DA and AA,and lower RC than non-ultra-drying seeds.Therefore,ultra-drying to 42 g·kg-1 of MC was helpful for M.suaveolens seed storage.
文摘The metabolism of fats, proteins and carbohydrates and the change of enzyme activity in sceds of Sophora japonica during germination were studied by methods of Soxhlet's extraction, flow injection and colorimctric analysis of speetrophotometer for providing theoretical basis for germination and storage of seeds of forest trees. The results arc as follows:(1)The activity of enzymes in the seeds does not always coincide with the increase or deercase of their corresponding substances during germination of the seeds of Sophora japonica.(2)Proteins were first utilized during germination of the seeds.(3)The fatty acids reducing sugar and amino acids in the radiele+plumular axis were used pseedcntly over all others when the embryos grew.(4)excessive small-molecular substances produced by hydrolysis would accumulate in tissres and sometimes they were stored temporarily in the form of storage substances and finally used for embryo growth.
文摘Factors affecting the in vitro germination and growth of Taxus cuspidata embryos were examined. DCR medium was the best among 6 basal media tested; embryos from stage II seeds were the optimal developmental stage for in vitro germination; as the seeds approached maturity, germinability of embryos decrease. Embryos cultured in darkness tended to form callus and decrease the frequency of germination; inoculation method obviously affected frequency of embryo germination. Embryos cultured in optimal conditions developed into seedlings in 2 months.
