Essential oil, with more than thirty kinds of compounds separated and identified by gas chromatography-mass spectrometry, was extracted from Shatian shaddock peel and Sweet shaddock peel by squeeze-steam distillation ...Essential oil, with more than thirty kinds of compounds separated and identified by gas chromatography-mass spectrometry, was extracted from Shatian shaddock peel and Sweet shaddock peel by squeeze-steam distillation and direct steam distillation method. Among their composition, the main components are terpene compounds, which account for 93.926% (mass fraction, the same below) and 85.843% of essential oils extracted from Shatian shaddock peel and Sweet shaddock peel, respectively. Although nootkatone is the major contributor of shaddock characteristic scent, and its contents are 1.069% and 1.749% of essential oils from Sweet shaddock peel and Shatian shaddock peel, respectively. The results show that squeeze-steam distillation gives higher yield and good quality of essential oil and the compositions of essential oils from two kinds of shaddock peels are different, but the main contributors of the shaddock scent are the same.展开更多
A gas chromatographic method for determining the trace residue of imazethapyr {(±)-2-[4,5-dihydro-4-methtyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-ehtyl-3-pyridinecarboxylic acid} in soil was described in th...A gas chromatographic method for determining the trace residue of imazethapyr {(±)-2-[4,5-dihydro-4-methtyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-ehtyl-3-pyridinecarboxylic acid} in soil was described in this paper. The soil samples were extracted by shaking with 0.5 mol·L -1 sodium hydroxide solution. The aqueous extract was acidified with hydrocholric acid to adjust the pH to 2.0 to precipitate humic acids, then was extracted with dichloromethane. The organic extract was dried with anhydrous sodium sulfate and was concentrated. After florisil cleanup, and imazethapyr is derivatized with trimethylanilinium hydroxide (TMAH). Methylated imazethapyr was analyzed by gas chromatography with a nitrogen-phosphorous dtector. The recovery of imazethapyr from soil samples are over 80%, the experimental detection limit of the method is 5ng·g -1 .展开更多
Three new chiral stationary phases, 2,6 di O heptyl 3 O trimethylsilyl β cyclodextrin(DHTBCD), 2,6 di O pentyl 3 O trimethylsilyl β cyclodextrin (DPTBCD) and 2,6 di O butyl 3 O trimethylsilyl β cyclodextrin (DBTBCD...Three new chiral stationary phases, 2,6 di O heptyl 3 O trimethylsilyl β cyclodextrin(DHTBCD), 2,6 di O pentyl 3 O trimethylsilyl β cyclodextrin (DPTBCD) and 2,6 di O butyl 3 O trimethylsilyl β cyclodextrin (DBTBCD), were synthesized. Chromatographic properties such as column efficiency, thermal stability and column life span, were studied. The separations of enantiomers, such as ketone, esters, alcohols and olefines, were investigated on the alkylated and silylated β cyclodextrin stationary phases. The influence of diluent on chiral separation was studied. The experimental results indicate that the stationary phases show good chromatographic properties in separating enantiomers. It is observed that inclusion complexation and hydrogen bonding interaction hardly play a role in separating enantiomers.展开更多
采用顶空固相微萃取(headspace solid-phase microextraction,HS-SPME)和液液萃取(liquid-liquid extraction,LLE)结合全二维气相色谱飞行时间质谱(comprehensive two-dimensional gas chromatography time of flight mass spectrometry...采用顶空固相微萃取(headspace solid-phase microextraction,HS-SPME)和液液萃取(liquid-liquid extraction,LLE)结合全二维气相色谱飞行时间质谱(comprehensive two-dimensional gas chromatography time of flight mass spectrometry,GC×GC-TOF-MS)以及香气活度值(odour active value,OAV),对红星二锅头白酒的挥发性成分进行全面解析。研究发现,HS-SPME、LLE分别定性出928、802种挥发性化合物,共计定性出1304种挥发性化合物,共同定性出426种挥发性化合物。基于HS-SPME数据,通过香气数据库筛选出具有香气特征的382种香气化合物,其中酯类相对百分含量占比最高,其次是醇类、酸类和醛类。计算得到了49种香气化合物OAV>1,其中酯类(辛酸乙酯、异戊酸乙酯等)和萜烯类(β-大马酮)对白酒风味的贡献最大,醛类(异戊醛、己醛等)和含硫类(二甲基三硫)其次,醇类(1-辛烯-3-醇)和含氮类(2,3,5-三甲基吡嗪)也有一定风味贡献。该研究丰富了红星二锅头白酒的风味研究,也为下一步生产研究及调控提供了理论和数据支撑。展开更多
To establish a new GC-MS/MS method for quantitative detection of 22 kinds of pesticide in fruit and vegetable including organic phosphorus,carbamate & pyrethrin etc.Working was performed by Xcalibur software with ...To establish a new GC-MS/MS method for quantitative detection of 22 kinds of pesticide in fruit and vegetable including organic phosphorus,carbamate & pyrethrin etc.Working was performed by Xcalibur software with multitask mode.We Carried on qualitative and quantitative analysis to mass spectrum of MS/MS in order to obtained a credible experiment result.This method is sensitive and accurate,it is suitable for determination of multifarious pesticide residues.展开更多
Poly brominated biphenyl(PBBs) and poly brominated diphenyl ether(PBDEs) in polymers of electrotechnical products have been extracted with toluene and analyzed by gas chromatography-mass spectrometry (GC-MS).The resul...Poly brominated biphenyl(PBBs) and poly brominated diphenyl ether(PBDEs) in polymers of electrotechnical products have been extracted with toluene and analyzed by gas chromatography-mass spectrometry (GC-MS).The result has more credibility when the nature of the sample was determined by retention time and the proportion of characteristic ions.PBBs and PBDEs were quantified by internal standard method and 2′,3,3′,4,4′,5,5′,6,6′-decachlorobiphenyl was used as internal standard.The GC-MS experimental results show,that the average spiked recoveries ranged from 81% to 110%,that the relative standard deviations(RSD) ranged from 1.54% to 8.65%.The research results also showed that the method presented simplicity-operating,excellent linearity and reproducibility.展开更多
Labeled polychlorinated dibenzo-p-dioxins and dibenzofurans(PCDD/Fs)were extracted by three different methods,i.e.,soxhlet extraction,hot extraction and accelerated solvent extraction(ASE).The PCDD/Fs were detected by...Labeled polychlorinated dibenzo-p-dioxins and dibenzofurans(PCDD/Fs)were extracted by three different methods,i.e.,soxhlet extraction,hot extraction and accelerated solvent extraction(ASE).The PCDD/Fs were detected by high resolution gas chromatography-high resolution mass spectrometry.Comparisons of the three methods were carried out by recovery of PCDD/Fs,solvent consumption and extraction time.The results showed that all of the method could extract labeled PCDD/Fs efficiently.ASE was a time saving procedure with lowest consumption of solvents compared with the other two methods.展开更多
To study the detection method of trimethyltin chloride in urine.Extraction trimethyltin chloride in urine by ethyl acetate,and detection by capillary gas chromatography and mass spectrometry method (GC-MS).The linear ...To study the detection method of trimethyltin chloride in urine.Extraction trimethyltin chloride in urine by ethyl acetate,and detection by capillary gas chromatography and mass spectrometry method (GC-MS).The linear range of trimethyltin chloride in the method was 0.776-19.4 μg/mL,the correlation coefficient was 0.999 9,the lowest detection concentration was 0.01 mg/L.Relative standard deviation(RSD) was 4.4%-8.6%,the recovery rates were 99.0%-110.0%,sample could be save in -20℃ at least one half year.The method was applicable to detect trimethyltin chloride in urine.展开更多
Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volum...Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volume integration.By means of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.In total,97,62,and 78 volatile chemical components in volatile oil of HE,RC,and DP HE-RC,were respectively determined qualitatively and quantitatively,accounting for 90.08%,91.62%,and 89.76% total contents of volatile oil of HE,RC,and DP HE-RC respectively.It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo[4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol and(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.展开更多
As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus s...As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus sp. B-8, a newly isolated strain, was studied. This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source, including guaiacol, veratric acid, vanillic acid, cinnamic acid, p-coumaric acid, ferulic acid, and sinapic acid. In addition, the effects of different concentrations of ferulic acid on growth of Cupriavidus sp. B-8 were studied. The growth of Cupriavidus sp. B-8 is better under the condition of lower concentration. High-performance liquid chromatography （HPLC） analysis reveals that above 95% of ferulic acid is degraded within 12 h by Cupriavidus sp. B-8. Based on identification of biodegradation intermediates and further metabolites, the biodegradation pathway of ferulic acid by Cupriavidus sp. B-8 was proposed. Ferulic acid is initially converted to 4-vinylguaiacol, and further oxidized to vanillic acid and protocatechuic acid.展开更多
Propamocarb （PM） residue in cucumber （Cucumis sativus L.） receives little attention. As is well known to all, high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and...Propamocarb （PM） residue in cucumber （Cucumis sativus L.） receives little attention. As is well known to all, high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and ultimately cause harm to human health. Knowledge of PM residue could help cucumber breeders in developing cultivars with low PM residue and improving cucumber quality. In this study, 32 representative cucumber accessions （26 breeding lines and six cultivars） from different regions of China were evaluated for their PM residue in fruit and leaf to provide meaning to the subjective rating, which was highly correlated with PM residual content of fruit （r=0.97） and leaf （r=0.94）. In addition, PM residual content of North China ecotype was the highest and Pickling ecotype was the lowest in fruit and leaf of cucumber. The leaf had significantly higher （P〈0.01） PM residual content than the fruit, and poor correlation between leaf and fruit was represented. This study verified PM residual relationship between fruit and leaf, and laid the foundation for further identification of germplasm resources and breeding of new varieties for low PM residue of cucumber.展开更多
An insoluble β-cyclodextrin polymer cross-linked with epichlorohydrin was prepared, and its structure was identified with infrared spectrum. Colloid stationary phase was prepared by dissolving the polymer in the mixe...An insoluble β-cyclodextrin polymer cross-linked with epichlorohydrin was prepared, and its structure was identified with infrared spectrum. Colloid stationary phase was prepared by dissolving the polymer in the mixed solvent of diisopropyl ether, methylene dichloride and benzene and treated for 0.5 h by ultrasonication, and then was coated on a fused silica capillary column. The optimun reaction conditions are as follows: the mole ratio of epichlohydrin to β-cyclodextrin is 12.1:1, reacting at 65 °C for 24 h. The Chromatographic performance such as column efficiency, thermal stabilities and polarity were studied, two kinds of disubstituted benzene isomers and eight pairs of enantiomers were separated on the capillary column. The results show that the β-cyclodextrin polymer is suitable for use as a capillary gas chromatographic stationary phase, and possess excellent chromatographic properties in separating enantiomers and position isomers.展开更多
The detector,as well as being an essential supporting device for the gas chromatography(GC) has also played a critical role in the development of the technique as a whole.The mass spectrometer(MS) is still the commonl...The detector,as well as being an essential supporting device for the gas chromatography(GC) has also played a critical role in the development of the technique as a whole.The mass spectrometer(MS) is still the commonly praised detector as before.In fact,the information of fragmentation patterns is seldom used in practice,and the GC-MS instrument is even more expensive.For today's analytical problems,it seems that element specific detectors can and should be used for many applications rather than GC-MS.展开更多
By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina (Arthrospira) maxima that can be bio functionally healthy components were fractionated in four parts including the c...By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina (Arthrospira) maxima that can be bio functionally healthy components were fractionated in four parts including the culture medium (PSCM), the external layers of the cell (PSEL), the cell wall (PSCW) and storage granules (PSSG). The four fractionated parts of polysaccharides were characterized by gas liquid chromatography (GLC) equipped with FID and fused silica capillary column (15 m×0.53 mm i. d.). The contents of PSCM, PSEL, PSCW and PSSG were about 2.0%, 2.6%, 10.0% and 52.0% to cell dry matter, respectively. Glucose was almost the only monosaccharide in PSSG and PSCW and most predominant in PSEL, while in PSCM, xylose, rhamnose and glucose were the main ones. Two uronic acids represented by glucuronic acid and galacturoic acid, six neutral monosaccharides including fucose, rhamnose, xylose, mannose, galactose and glucose, and two possible unidentified sugars were found in PSEL and PSCM. These data are valuable for the selective productions of high added value sugars from Spirulina .展开更多
文摘Essential oil, with more than thirty kinds of compounds separated and identified by gas chromatography-mass spectrometry, was extracted from Shatian shaddock peel and Sweet shaddock peel by squeeze-steam distillation and direct steam distillation method. Among their composition, the main components are terpene compounds, which account for 93.926% (mass fraction, the same below) and 85.843% of essential oils extracted from Shatian shaddock peel and Sweet shaddock peel, respectively. Although nootkatone is the major contributor of shaddock characteristic scent, and its contents are 1.069% and 1.749% of essential oils from Sweet shaddock peel and Shatian shaddock peel, respectively. The results show that squeeze-steam distillation gives higher yield and good quality of essential oil and the compositions of essential oils from two kinds of shaddock peels are different, but the main contributors of the shaddock scent are the same.
文摘A gas chromatographic method for determining the trace residue of imazethapyr {(±)-2-[4,5-dihydro-4-methtyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-ehtyl-3-pyridinecarboxylic acid} in soil was described in this paper. The soil samples were extracted by shaking with 0.5 mol·L -1 sodium hydroxide solution. The aqueous extract was acidified with hydrocholric acid to adjust the pH to 2.0 to precipitate humic acids, then was extracted with dichloromethane. The organic extract was dried with anhydrous sodium sulfate and was concentrated. After florisil cleanup, and imazethapyr is derivatized with trimethylanilinium hydroxide (TMAH). Methylated imazethapyr was analyzed by gas chromatography with a nitrogen-phosphorous dtector. The recovery of imazethapyr from soil samples are over 80%, the experimental detection limit of the method is 5ng·g -1 .
文摘Three new chiral stationary phases, 2,6 di O heptyl 3 O trimethylsilyl β cyclodextrin(DHTBCD), 2,6 di O pentyl 3 O trimethylsilyl β cyclodextrin (DPTBCD) and 2,6 di O butyl 3 O trimethylsilyl β cyclodextrin (DBTBCD), were synthesized. Chromatographic properties such as column efficiency, thermal stability and column life span, were studied. The separations of enantiomers, such as ketone, esters, alcohols and olefines, were investigated on the alkylated and silylated β cyclodextrin stationary phases. The influence of diluent on chiral separation was studied. The experimental results indicate that the stationary phases show good chromatographic properties in separating enantiomers. It is observed that inclusion complexation and hydrogen bonding interaction hardly play a role in separating enantiomers.
文摘采用顶空固相微萃取(headspace solid-phase microextraction,HS-SPME)和液液萃取(liquid-liquid extraction,LLE)结合全二维气相色谱飞行时间质谱(comprehensive two-dimensional gas chromatography time of flight mass spectrometry,GC×GC-TOF-MS)以及香气活度值(odour active value,OAV),对红星二锅头白酒的挥发性成分进行全面解析。研究发现,HS-SPME、LLE分别定性出928、802种挥发性化合物,共计定性出1304种挥发性化合物,共同定性出426种挥发性化合物。基于HS-SPME数据,通过香气数据库筛选出具有香气特征的382种香气化合物,其中酯类相对百分含量占比最高,其次是醇类、酸类和醛类。计算得到了49种香气化合物OAV>1,其中酯类(辛酸乙酯、异戊酸乙酯等)和萜烯类(β-大马酮)对白酒风味的贡献最大,醛类(异戊醛、己醛等)和含硫类(二甲基三硫)其次,醇类(1-辛烯-3-醇)和含氮类(2,3,5-三甲基吡嗪)也有一定风味贡献。该研究丰富了红星二锅头白酒的风味研究,也为下一步生产研究及调控提供了理论和数据支撑。
文摘To establish a new GC-MS/MS method for quantitative detection of 22 kinds of pesticide in fruit and vegetable including organic phosphorus,carbamate & pyrethrin etc.Working was performed by Xcalibur software with multitask mode.We Carried on qualitative and quantitative analysis to mass spectrum of MS/MS in order to obtained a credible experiment result.This method is sensitive and accurate,it is suitable for determination of multifarious pesticide residues.
文摘Poly brominated biphenyl(PBBs) and poly brominated diphenyl ether(PBDEs) in polymers of electrotechnical products have been extracted with toluene and analyzed by gas chromatography-mass spectrometry (GC-MS).The result has more credibility when the nature of the sample was determined by retention time and the proportion of characteristic ions.PBBs and PBDEs were quantified by internal standard method and 2′,3,3′,4,4′,5,5′,6,6′-decachlorobiphenyl was used as internal standard.The GC-MS experimental results show,that the average spiked recoveries ranged from 81% to 110%,that the relative standard deviations(RSD) ranged from 1.54% to 8.65%.The research results also showed that the method presented simplicity-operating,excellent linearity and reproducibility.
文摘Labeled polychlorinated dibenzo-p-dioxins and dibenzofurans(PCDD/Fs)were extracted by three different methods,i.e.,soxhlet extraction,hot extraction and accelerated solvent extraction(ASE).The PCDD/Fs were detected by high resolution gas chromatography-high resolution mass spectrometry.Comparisons of the three methods were carried out by recovery of PCDD/Fs,solvent consumption and extraction time.The results showed that all of the method could extract labeled PCDD/Fs efficiently.ASE was a time saving procedure with lowest consumption of solvents compared with the other two methods.
文摘To study the detection method of trimethyltin chloride in urine.Extraction trimethyltin chloride in urine by ethyl acetate,and detection by capillary gas chromatography and mass spectrometry method (GC-MS).The linear range of trimethyltin chloride in the method was 0.776-19.4 μg/mL,the correlation coefficient was 0.999 9,the lowest detection concentration was 0.01 mg/L.Relative standard deviation(RSD) was 4.4%-8.6%,the recovery rates were 99.0%-110.0%,sample could be save in -20℃ at least one half year.The method was applicable to detect trimethyltin chloride in urine.
基金Project (01962502) supported by the Natural Science Foundation of Hunan Province, China
文摘Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volume integration.By means of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.In total,97,62,and 78 volatile chemical components in volatile oil of HE,RC,and DP HE-RC,were respectively determined qualitatively and quantitatively,accounting for 90.08%,91.62%,and 89.76% total contents of volatile oil of HE,RC,and DP HE-RC respectively.It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo[4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol and(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.
基金Foundation item： Project（50925417） supported by the National Science Fund for Distinguished Young Scholars of China Project（50830301） supported by the National Natural Science Foundation of China Project（2011467062） supported by National Research Fund for Public Benefit （Environmental Protection） Industries, China
文摘As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus sp. B-8, a newly isolated strain, was studied. This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source, including guaiacol, veratric acid, vanillic acid, cinnamic acid, p-coumaric acid, ferulic acid, and sinapic acid. In addition, the effects of different concentrations of ferulic acid on growth of Cupriavidus sp. B-8 were studied. The growth of Cupriavidus sp. B-8 is better under the condition of lower concentration. High-performance liquid chromatography （HPLC） analysis reveals that above 95% of ferulic acid is degraded within 12 h by Cupriavidus sp. B-8. Based on identification of biodegradation intermediates and further metabolites, the biodegradation pathway of ferulic acid by Cupriavidus sp. B-8 was proposed. Ferulic acid is initially converted to 4-vinylguaiacol, and further oxidized to vanillic acid and protocatechuic acid.
基金Supported by the National Natural Science Foundation of China(31272158)863 Program of the National Science and Technology of China(2012AA100105)
文摘Propamocarb （PM） residue in cucumber （Cucumis sativus L.） receives little attention. As is well known to all, high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and ultimately cause harm to human health. Knowledge of PM residue could help cucumber breeders in developing cultivars with low PM residue and improving cucumber quality. In this study, 32 representative cucumber accessions （26 breeding lines and six cultivars） from different regions of China were evaluated for their PM residue in fruit and leaf to provide meaning to the subjective rating, which was highly correlated with PM residual content of fruit （r=0.97） and leaf （r=0.94）. In addition, PM residual content of North China ecotype was the highest and Pickling ecotype was the lowest in fruit and leaf of cucumber. The leaf had significantly higher （P〈0.01） PM residual content than the fruit, and poor correlation between leaf and fruit was represented. This study verified PM residual relationship between fruit and leaf, and laid the foundation for further identification of germplasm resources and breeding of new varieties for low PM residue of cucumber.
文摘An insoluble β-cyclodextrin polymer cross-linked with epichlorohydrin was prepared, and its structure was identified with infrared spectrum. Colloid stationary phase was prepared by dissolving the polymer in the mixed solvent of diisopropyl ether, methylene dichloride and benzene and treated for 0.5 h by ultrasonication, and then was coated on a fused silica capillary column. The optimun reaction conditions are as follows: the mole ratio of epichlohydrin to β-cyclodextrin is 12.1:1, reacting at 65 °C for 24 h. The Chromatographic performance such as column efficiency, thermal stabilities and polarity were studied, two kinds of disubstituted benzene isomers and eight pairs of enantiomers were separated on the capillary column. The results show that the β-cyclodextrin polymer is suitable for use as a capillary gas chromatographic stationary phase, and possess excellent chromatographic properties in separating enantiomers and position isomers.
文摘The detector,as well as being an essential supporting device for the gas chromatography(GC) has also played a critical role in the development of the technique as a whole.The mass spectrometer(MS) is still the commonly praised detector as before.In fact,the information of fragmentation patterns is seldom used in practice,and the GC-MS instrument is even more expensive.For today's analytical problems,it seems that element specific detectors can and should be used for many applications rather than GC-MS.
文摘By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina (Arthrospira) maxima that can be bio functionally healthy components were fractionated in four parts including the culture medium (PSCM), the external layers of the cell (PSEL), the cell wall (PSCW) and storage granules (PSSG). The four fractionated parts of polysaccharides were characterized by gas liquid chromatography (GLC) equipped with FID and fused silica capillary column (15 m×0.53 mm i. d.). The contents of PSCM, PSEL, PSCW and PSSG were about 2.0%, 2.6%, 10.0% and 52.0% to cell dry matter, respectively. Glucose was almost the only monosaccharide in PSSG and PSCW and most predominant in PSEL, while in PSCM, xylose, rhamnose and glucose were the main ones. Two uronic acids represented by glucuronic acid and galacturoic acid, six neutral monosaccharides including fucose, rhamnose, xylose, mannose, galactose and glucose, and two possible unidentified sugars were found in PSEL and PSCM. These data are valuable for the selective productions of high added value sugars from Spirulina .
