To investigate the frost-heave properties of silty clay under the combination action of seasonal freezing and artificial freezing, and verify the feasibility of combined freezing, eight combined freezing experiments w...To investigate the frost-heave properties of silty clay under the combination action of seasonal freezing and artificial freezing, and verify the feasibility of combined freezing, eight combined freezing experiments were performed on silty clay with water content (mass fraction) of 23.5% and 28.0%, through developed frost-heave test apparatus, in closed or open system. Two sorts of freezing temperature models, namely, constant and sine models, were applied to artificial freezing. The experimental results indicate that the frost-heave degree in seasonal freezing stage accounts for over 90% of the total in open system and it is up to 95% in closed system; the change of artificial sine-freezing temperature has no influence on the frost-heave degree in closed system, however, slight influence in open system. It is found that the variation of temperature gradient of sine-freezing specimen lags behind that of sine-freezing temperature with half phase; sine-freezing temperature model can reduce frost-heave degree of soil. Brand new technology is proposed for the application of artificial ground freezing and new study field of artificial freezing is created.展开更多
Winter wheat （Triticum aestivum） cultivars Dongnongdongmai 1 with strong cold/freezing tolerance and Jimai 22 with weak cold/freezing tolerance were used for investigating the difference of microstructure and ultras...Winter wheat （Triticum aestivum） cultivars Dongnongdongmai 1 with strong cold/freezing tolerance and Jimai 22 with weak cold/freezing tolerance were used for investigating the difference of microstructure and ultrastructure between leaves of two cultivars under low temperature stress （5℃ and -15 ℃） using optical and electron microscope. The results showed that there was no obvious difference between leaves of Dongnongdongmai 1 and Jimai 22 in microstructure. However, the difference between those leaves was distinct in ultrastructure. The grana lamella and stroma lamella were stacked regularly and arranged parallelly along the long axis of chloroplasts in cv. Dongnongdongmai 1, while the arrangement directions of thylakoids in Jimai 22＇s leaves were so irregular as to form various angles with the long axis of chloroplasts. At -15℃, the mitochondrias were swelled to be round and the structure of cristaes became blurry in both cultivars＇ leaves, while some cristaes of Jimai 22 disappeared. These results would provide theoretical evidence for selecting cold/freezing tolerant winter wheat germplasm resources展开更多
A novel vacuum ice slurry producing system with jet-pumps was proposed to deal with the problems of high energy consumption and ice blockage.In this novel system,one steam driven by a jet-pump was used to create vacuu...A novel vacuum ice slurry producing system with jet-pumps was proposed to deal with the problems of high energy consumption and ice blockage.In this novel system,one steam driven by a jet-pump was used to create vacuum in a hermetic vessel where water was sprayed through a nozzle to produce ice slurry,while the other steam was used to provide enough cold energy to make the left vapor in the hermetic vessel condense.Mathematical models of this novel system were established and theoretical simulation on the performance characteristics was also implemented based on the MATLAB program.Results show that the novel system is feasible and practicable,and the system performance is affected by many factors,such as the temperature of the generators,condensing temperature,evaporation temperature,and the cooling load of the refrigerator sub-system.The findings are helpful to improve the performance of ice slurry producing system.展开更多
A two-dimensional model for freezing and thawing phase change heat transfer in biological tissue embedded with two cryoprobes was established.In this model,the blood vessels were considered as tree-like branched fract...A two-dimensional model for freezing and thawing phase change heat transfer in biological tissue embedded with two cryoprobes was established.In this model,the blood vessels were considered as tree-like branched fractal network,and the effective flow rate and effective thermal conductivity of blood were obtained by fractal method.The temperature distribution and ice crystal growth process in biological tissue embedded with two cryoprobes during freezing-thawing process were numerically simulated.The results show that the growth velocity of ice crystal in freezing process from 200 to 400 s is more rapid than that from 400 to 600 s. Thawing process of frozen tissue occurs in the regions around cryoprobes tips and tissue boundary simultaneously,and the phase interfaces are close to each other until ice crystal melts completely.The distance of two cryoprobes has a more profound effect on the temperature distribution in freezing process at 400 s than at 800 s.展开更多
In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen i...In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 （P0.01）. The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method （P0.01）. The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI.展开更多
As one of the major projects of GAME (GEWEX Asian Monsoon Experiment), the GAME\|Tibet aimed to mainly examine the energy and water cycle in Tibetan Plateau and its effects on Asian monsoon. In this paper, based on th...As one of the major projects of GAME (GEWEX Asian Monsoon Experiment), the GAME\|Tibet aimed to mainly examine the energy and water cycle in Tibetan Plateau and its effects on Asian monsoon. In this paper, based on the in\|situ high\|resolution observation data of GAME\|Tibet, the soil energy\|moisture distribution and the melting\|freezing progresses and their effects on seasonal shift were preliminarily discussed.The soil energy\|water distribution and freezing\|melting processes varied at different sites in northern part of Tibetan Plateau. The temporal and spatial variation of the soil moisture content is more complex than that of temperature. The soil moisture content increased with depth in certain layers but decreased in other layers. The freezing and melting processes and the temperature distribution were largely influenced by the existence of higher soil moisture content layer. During summer monsoon, the soil moisture at 10cm at all sites is relatively high, but the spatial difference existed. Generally speaking, the shallow layers start to freeze in October and to melt from April at all sites, with about 6 months frozen period. However, the beginning time of freezing\|melting and frozen period varied at different sites.展开更多
Probiotics which can provide potential health benefits for consumers and prevent disease, is the most important research field for functional food in the future. It is the key point that how much the count of bacteria...Probiotics which can provide potential health benefits for consumers and prevent disease, is the most important research field for functional food in the future. It is the key point that how much the count of bacteria and how long it can preserve. Freeze-drying skill is one of the best preservation methods, but it＇s defect that damage to biological systems can be attributed to changes in the physical state of membrane lipids or/and changes in the structure of sensitive proteins and the decreased survival rate. This work used pretreatment methods-cold shock and hot shock to reduce the damage to bacteria during freeze-drying. Using unpretreated samples as control, aspects of cold shock or heat shock. The response of Enterococcous faecalis A31 was investigated during lag phase, the middle of exponential growth phase and the terminal of exponential growth phase. The results suggested that when cold shock, the shock protein concentration produced by treated sample within 2 h during the middle of exponential growth phase was higher than 4, 8, 24 h, The concentration of shock protein produced by treated sample at 10 ℃ was higher than 20℃, The concentration of sample protein at the middle of exponential growth phase was higher than that at the terminal of exponential growth phase. After one-month storage, the survival rate at 10℃ was better than 20 ℃ and 4 ℃comparatively. The survival rate at （6 h） 4 ℃/24 h （lag period） was higher than （10 h） 4 ℃/24 h （the middle of exponential growth phase）, and the effect of 10℃/8 h shock treatments was best during the middle of exponential growth phase, when hot shock, The concentration of heat shock proteins produced was not obvious, and survival rate was higher at 45 ℃, 30 min than at 45 ℃, 60 min.展开更多
The iron concentrations of snows,semi-melting snows,snowmelts,and ditch waters were observed in four typical microhabitats,Carex lasiocarpa marsh(Ⅰ), Calamagrostis angustifolia wet meadow(Ⅱ),dry land (Ⅲ)and paddy f...The iron concentrations of snows,semi-melting snows,snowmelts,and ditch waters were observed in four typical microhabitats,Carex lasiocarpa marsh(Ⅰ), Calamagrostis angustifolia wet meadow(Ⅱ),dry land (Ⅲ)and paddy field(Ⅳ),of Sanjiang Plain Wetland, Northeast China.Each sample was collected from three sites of one microhabitat,mixed together, filtrated with 0.45μm membrane,and tested using atomic absorption spectrometry(AAS)for iron measurement.The iron concentrations of soil solutions were investigated as well.Each soil solution was in-situ extracted by negative pressure,filtrated with 0.45μm membrane,and tested using AAS,too. The results showed that the wet precipitation of iron from snow were little to detect.The loss of iron was attributed to the interaction of water and soil surface. The iron concentrations of snowmelts were 7.4,15,展开更多
基金Project(40571032) supported by the National Natural Science Foundation of ChinaProject(2006G011-B-3) supported by Science Studies and Development Plan Foundation of Railway Ministry
文摘To investigate the frost-heave properties of silty clay under the combination action of seasonal freezing and artificial freezing, and verify the feasibility of combined freezing, eight combined freezing experiments were performed on silty clay with water content (mass fraction) of 23.5% and 28.0%, through developed frost-heave test apparatus, in closed or open system. Two sorts of freezing temperature models, namely, constant and sine models, were applied to artificial freezing. The experimental results indicate that the frost-heave degree in seasonal freezing stage accounts for over 90% of the total in open system and it is up to 95% in closed system; the change of artificial sine-freezing temperature has no influence on the frost-heave degree in closed system, however, slight influence in open system. It is found that the variation of temperature gradient of sine-freezing specimen lags behind that of sine-freezing temperature with half phase; sine-freezing temperature model can reduce frost-heave degree of soil. Brand new technology is proposed for the application of artificial ground freezing and new study field of artificial freezing is created.
基金Supported by Doctoral Scientific Research Project of Northeast Agricultural University (20082010)Innovative Research Team Project of Northeast Agricultural University (CXZ003)
文摘Winter wheat （Triticum aestivum） cultivars Dongnongdongmai 1 with strong cold/freezing tolerance and Jimai 22 with weak cold/freezing tolerance were used for investigating the difference of microstructure and ultrastructure between leaves of two cultivars under low temperature stress （5℃ and -15 ℃） using optical and electron microscope. The results showed that there was no obvious difference between leaves of Dongnongdongmai 1 and Jimai 22 in microstructure. However, the difference between those leaves was distinct in ultrastructure. The grana lamella and stroma lamella were stacked regularly and arranged parallelly along the long axis of chloroplasts in cv. Dongnongdongmai 1, while the arrangement directions of thylakoids in Jimai 22＇s leaves were so irregular as to form various angles with the long axis of chloroplasts. At -15℃, the mitochondrias were swelled to be round and the structure of cristaes became blurry in both cultivars＇ leaves, while some cristaes of Jimai 22 disappeared. These results would provide theoretical evidence for selecting cold/freezing tolerant winter wheat germplasm resources
基金Project(51376198)supported by the National Natural Science Foundation of ChinaProject(11JJ22029)supported by the Hunan Provincial Natural Science Foundation of China
文摘A novel vacuum ice slurry producing system with jet-pumps was proposed to deal with the problems of high energy consumption and ice blockage.In this novel system,one steam driven by a jet-pump was used to create vacuum in a hermetic vessel where water was sprayed through a nozzle to produce ice slurry,while the other steam was used to provide enough cold energy to make the left vapor in the hermetic vessel condense.Mathematical models of this novel system were established and theoretical simulation on the performance characteristics was also implemented based on the MATLAB program.Results show that the novel system is feasible and practicable,and the system performance is affected by many factors,such as the temperature of the generators,condensing temperature,evaporation temperature,and the cooling load of the refrigerator sub-system.The findings are helpful to improve the performance of ice slurry producing system.
基金Project(50436030)supported by the National Natural Science Foundation of China
文摘A two-dimensional model for freezing and thawing phase change heat transfer in biological tissue embedded with two cryoprobes was established.In this model,the blood vessels were considered as tree-like branched fractal network,and the effective flow rate and effective thermal conductivity of blood were obtained by fractal method.The temperature distribution and ice crystal growth process in biological tissue embedded with two cryoprobes during freezing-thawing process were numerically simulated.The results show that the growth velocity of ice crystal in freezing process from 200 to 400 s is more rapid than that from 400 to 600 s. Thawing process of frozen tissue occurs in the regions around cryoprobes tips and tissue boundary simultaneously,and the phase interfaces are close to each other until ice crystal melts completely.The distance of two cryoprobes has a more profound effect on the temperature distribution in freezing process at 400 s than at 800 s.
文摘In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 （P0.01）. The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method （P0.01）. The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI.
文摘As one of the major projects of GAME (GEWEX Asian Monsoon Experiment), the GAME\|Tibet aimed to mainly examine the energy and water cycle in Tibetan Plateau and its effects on Asian monsoon. In this paper, based on the in\|situ high\|resolution observation data of GAME\|Tibet, the soil energy\|moisture distribution and the melting\|freezing progresses and their effects on seasonal shift were preliminarily discussed.The soil energy\|water distribution and freezing\|melting processes varied at different sites in northern part of Tibetan Plateau. The temporal and spatial variation of the soil moisture content is more complex than that of temperature. The soil moisture content increased with depth in certain layers but decreased in other layers. The freezing and melting processes and the temperature distribution were largely influenced by the existence of higher soil moisture content layer. During summer monsoon, the soil moisture at 10cm at all sites is relatively high, but the spatial difference existed. Generally speaking, the shallow layers start to freeze in October and to melt from April at all sites, with about 6 months frozen period. However, the beginning time of freezing\|melting and frozen period varied at different sites.
基金Supported by National Agricultural Translation Fund (02EFN212301073)
文摘Probiotics which can provide potential health benefits for consumers and prevent disease, is the most important research field for functional food in the future. It is the key point that how much the count of bacteria and how long it can preserve. Freeze-drying skill is one of the best preservation methods, but it＇s defect that damage to biological systems can be attributed to changes in the physical state of membrane lipids or/and changes in the structure of sensitive proteins and the decreased survival rate. This work used pretreatment methods-cold shock and hot shock to reduce the damage to bacteria during freeze-drying. Using unpretreated samples as control, aspects of cold shock or heat shock. The response of Enterococcous faecalis A31 was investigated during lag phase, the middle of exponential growth phase and the terminal of exponential growth phase. The results suggested that when cold shock, the shock protein concentration produced by treated sample within 2 h during the middle of exponential growth phase was higher than 4, 8, 24 h, The concentration of shock protein produced by treated sample at 10 ℃ was higher than 20℃, The concentration of sample protein at the middle of exponential growth phase was higher than that at the terminal of exponential growth phase. After one-month storage, the survival rate at 10℃ was better than 20 ℃ and 4 ℃comparatively. The survival rate at （6 h） 4 ℃/24 h （lag period） was higher than （10 h） 4 ℃/24 h （the middle of exponential growth phase）, and the effect of 10℃/8 h shock treatments was best during the middle of exponential growth phase, when hot shock, The concentration of heat shock proteins produced was not obvious, and survival rate was higher at 45 ℃, 30 min than at 45 ℃, 60 min.
文摘The iron concentrations of snows,semi-melting snows,snowmelts,and ditch waters were observed in four typical microhabitats,Carex lasiocarpa marsh(Ⅰ), Calamagrostis angustifolia wet meadow(Ⅱ),dry land (Ⅲ)and paddy field(Ⅳ),of Sanjiang Plain Wetland, Northeast China.Each sample was collected from three sites of one microhabitat,mixed together, filtrated with 0.45μm membrane,and tested using atomic absorption spectrometry(AAS)for iron measurement.The iron concentrations of soil solutions were investigated as well.Each soil solution was in-situ extracted by negative pressure,filtrated with 0.45μm membrane,and tested using AAS,too. The results showed that the wet precipitation of iron from snow were little to detect.The loss of iron was attributed to the interaction of water and soil surface. The iron concentrations of snowmelts were 7.4,15,
