Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the ...Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.展开更多
Traditional manufacturing processes for lightweight curved profiles are often associated with lengthy procedures,high costs,low efficiency,and high energy consumption.In order to solve this problem,a new staggered ext...Traditional manufacturing processes for lightweight curved profiles are often associated with lengthy procedures,high costs,low efficiency,and high energy consumption.In order to solve this problem,a new staggered extrusion(SE)process was used to form the curved profile of AZ31 magnesium alloy in this paper.The study investigates the mapping relationship between the curvature,microstructure,and mechanical properties of the formed profiles by using different eccentricities of the die.Scanning electron microscopy(SEM)and electron backscatter diffraction techniques are employed to examine the effects of different eccentricity values(e)on grain morphology,recrystallization mechanisms,texture,and Schmid factors of the products.The results demonstrate that the staggered extrusion method promotes the deep refinement of grain size in the extruded products,with an average grain size of only 15%of the original billet,reaching 12.28μm.The tensile strength and elongation of the curved profiles after extrusion under the eccentricity value of 10 mm,20 mm and 30 mm are significantly higher than those of the billet,with the tensile strength is increased to 250,270,235 MPa,and the engineering strain elongation increased to 10.5%,12.1%,15.9%.This indicates that staggered extrusion enables curvature control of the profiles while improving their strength.展开更多
Many compiler optimization techniques depend on which part code has been executed frequently. Profilingwill trace and record these information that a compiler needs automatically, which is useful to other phases durin...Many compiler optimization techniques depend on which part code has been executed frequently. Profilingwill trace and record these information that a compiler needs automatically, which is useful to other phases duringcompile. Profiling will instrument some internal code that gather these information during a program executes. For ex-ample, edge profiling computes the frequency of basic block and the probability of edge in control flow graph. Valueprofiling determine which variables have invariant behavior.This paper explores the basic block frequency found from edge profiling. We use the edge profiling to performsome optimization for Spec2000 test cases, showing that the optimization base edge profiling can reduce a program'sexecution time up to 5 %.展开更多
To extend our successful Magnetotelluric(MT) experiments in the area from Yadong to Bamocuo in 1995 (Chen et al., 1996), two new Magnetotelluric(MT)experiments have been carried out along two main profiles in the cent...To extend our successful Magnetotelluric(MT) experiments in the area from Yadong to Bamocuo in 1995 (Chen et al., 1996), two new Magnetotelluric(MT)experiments have been carried out along two main profiles in the central and Northern Tibetan Plateau in the summer of 1998 and 1999. While the 1995 MT work mainly focused on the study of the electrical structure of the Yarlung\|Zangbo River Suture , the 1998 and 1999 experiments have been designed with following purpose:(1) Study whether partially molten layer widely exists in the crust of the central and northern Tibet.(2) Study the electrical structures of crust and upper mantle in central and northern Tibet which may relate to the attenuated Shear Seismic waves.(3) Study the detail electrical structures of Bangong\|Nujiang Suture and Jinsha Suture.In 1998, the MT team (China University of Geosciences, University of Washington and Geological Survey of Canada) recorded MT data along the Tibet 500 Line which extends about three hundred and eighty kilometers from Deqing to Longweicuo. We used LIMS system to record the long period(20～20000s) MT data at twenty\|six sites and used MT24 to record broadband(320Hz,2000s) MT data at fifty\|eight sites.展开更多
Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls...Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls and which could play a role in controlling early fiber development.Chief among these was GhMYB25,a Mixta-like MYB gene.Transgenic GhMYB25-silenced cotton展开更多
Fasciclin-like arabinogalactan proteins(FLAs),a subclass of arabinogalactan proteins(AGPs),are usually involved in cell development in plants.To investigate the expression profiling as well
The first troposphere wind profiling radar in China has been in operation. The paper describes the radar parameters and characteristics with some experimental results presented.
Identifying the origin of body fluids left at a crime scene can give a significant insight into crime scene reconstruction by supporting a link between sample donors and actual criminal acts.However,the conventional b...Identifying the origin of body fluids left at a crime scene can give a significant insight into crime scene reconstruction by supporting a link between sample donors and actual criminal acts.However,the conventional body fluid identification methods are prone to various limitations,such as time consumption,intensive labor,nonparallel manner,varying degrees of sensitivity and limited specificity.Recently,the analysis of cell-specific messenger RNA expression(mRNA profiling)has been proposed to supplant conventional methods for body fluid identification.Since 2011,the collaborative exercises have been organized by the European DNA Profiling Group(EDNAP)in order to evaluate the robustness and reproducibility of mRNA profiling for body fluid identification.The major advantages of mRNA profiling,compared to the conventional methods,include higher sensitivity,greater specificity,the ability of detecting several body fluids in one multiplex reaction,and compatibility with current DNA extraction and analysis procedure.In the current review,we provided an overview of the present knowledge and detection methodologies of mRNA profiling for forensic body fluid identification and discussed its possible practical application to forensic casework.展开更多
In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed
The effect of signal sampling depth in SIMS on profile was not reported We have found that the maximum sampling depth in SIMS is considerably bigger than the mean penetration range of primary ions and can skew the pro...The effect of signal sampling depth in SIMS on profile was not reported We have found that the maximum sampling depth in SIMS is considerably bigger than the mean penetration range of primary ions and can skew the profile of secondary ion counts for implanted samples on the more deeper direction from the surface. The effect of maximum sampling depth is true not only for implanted samples but also for the samples in the middle of which there is an other impurity-rich layer. The action principle of signal sampling depth effect and the method of decreasing the error produced by the effect are discussed in this展开更多
This article investigates a multi-circular path-following formation control with reinforced transient profiles for nonholonomic vehicles connected by a digraph.A multi-circular formation controller endowed with the fe...This article investigates a multi-circular path-following formation control with reinforced transient profiles for nonholonomic vehicles connected by a digraph.A multi-circular formation controller endowed with the feature of spatial-temporal decoupling is devised for a group of vehicles guided by a virtual leader evolving along an implicit path,which allows for a circumnavigation on multiple circles with an anticipant angular spacing.In addition,notice that it typically imposes a stringent time constraint on time-sensitive enclosing scenarios,hence an improved prescribed performance control(IPPC)using novel tighter behavior boundaries is presented to enhance transient capabilities with an ensured appointed-time convergence free from any overshoots.The significant merits are that coordinated circumnavigation along different circles can be realized via executing geometric and dynamic assignments independently with modified transient profiles.Furthermore,all variables existing in the entire system are analyzed to be convergent.Simulation and experimental results are provided to validate the utility of suggested solution.展开更多
In the data-driven era of the internet and business environments,constructing accurate user profiles is paramount for personalized user understanding and classification.The traditional TF-IDF algorithm has some limita...In the data-driven era of the internet and business environments,constructing accurate user profiles is paramount for personalized user understanding and classification.The traditional TF-IDF algorithm has some limitations when evaluating the impact of words on classification results.Consequently,an improved TF-IDF-K algorithm was introduced in this study,which included an equalization factor,aimed at constructing user profiles by processing and analyzing user search records.Through the training and prediction capabilities of a Support Vector Machine(SVM),it enabled the prediction of user demographic attributes.The experimental results demonstrated that the TF-IDF-K algorithm has achieved a significant improvement in classification accuracy and reliability.展开更多
文摘Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.
基金Project(JQ2022E004)supported by the Natural Science Foundation of Heilongjiang Province,China。
文摘Traditional manufacturing processes for lightweight curved profiles are often associated with lengthy procedures,high costs,low efficiency,and high energy consumption.In order to solve this problem,a new staggered extrusion(SE)process was used to form the curved profile of AZ31 magnesium alloy in this paper.The study investigates the mapping relationship between the curvature,microstructure,and mechanical properties of the formed profiles by using different eccentricities of the die.Scanning electron microscopy(SEM)and electron backscatter diffraction techniques are employed to examine the effects of different eccentricity values(e)on grain morphology,recrystallization mechanisms,texture,and Schmid factors of the products.The results demonstrate that the staggered extrusion method promotes the deep refinement of grain size in the extruded products,with an average grain size of only 15%of the original billet,reaching 12.28μm.The tensile strength and elongation of the curved profiles after extrusion under the eccentricity value of 10 mm,20 mm and 30 mm are significantly higher than those of the billet,with the tensile strength is increased to 250,270,235 MPa,and the engineering strain elongation increased to 10.5%,12.1%,15.9%.This indicates that staggered extrusion enables curvature control of the profiles while improving their strength.
文摘Many compiler optimization techniques depend on which part code has been executed frequently. Profilingwill trace and record these information that a compiler needs automatically, which is useful to other phases duringcompile. Profiling will instrument some internal code that gather these information during a program executes. For ex-ample, edge profiling computes the frequency of basic block and the probability of edge in control flow graph. Valueprofiling determine which variables have invariant behavior.This paper explores the basic block frequency found from edge profiling. We use the edge profiling to performsome optimization for Spec2000 test cases, showing that the optimization base edge profiling can reduce a program'sexecution time up to 5 %.
文摘To extend our successful Magnetotelluric(MT) experiments in the area from Yadong to Bamocuo in 1995 (Chen et al., 1996), two new Magnetotelluric(MT)experiments have been carried out along two main profiles in the central and Northern Tibetan Plateau in the summer of 1998 and 1999. While the 1995 MT work mainly focused on the study of the electrical structure of the Yarlung\|Zangbo River Suture , the 1998 and 1999 experiments have been designed with following purpose:(1) Study whether partially molten layer widely exists in the crust of the central and northern Tibet.(2) Study the electrical structures of crust and upper mantle in central and northern Tibet which may relate to the attenuated Shear Seismic waves.(3) Study the detail electrical structures of Bangong\|Nujiang Suture and Jinsha Suture.In 1998, the MT team (China University of Geosciences, University of Washington and Geological Survey of Canada) recorded MT data along the Tibet 500 Line which extends about three hundred and eighty kilometers from Deqing to Longweicuo. We used LIMS system to record the long period(20～20000s) MT data at twenty\|six sites and used MT24 to record broadband(320Hz,2000s) MT data at fifty\|eight sites.
文摘Gene expression profiling at early stages(0～2 DPA) of fiber development in Gossypium hirsutum identified a number of transcription factors which were down regulated in fiberless mutants relative to wild type controls and which could play a role in controlling early fiber development.Chief among these was GhMYB25,a Mixta-like MYB gene.Transgenic GhMYB25-silenced cotton
文摘Fasciclin-like arabinogalactan proteins(FLAs),a subclass of arabinogalactan proteins(AGPs),are usually involved in cell development in plants.To investigate the expression profiling as well
文摘The first troposphere wind profiling radar in China has been in operation. The paper describes the radar parameters and characteristics with some experimental results presented.
基金supported by the grants from the National Key Technology Research&Development Program of the Ministry of Science and Technology of People’s Republic of China(2012BAK16B01)the National Natural Science Foundation of Peo-ple’s Republic of China(No.81222041,81172908)
文摘Identifying the origin of body fluids left at a crime scene can give a significant insight into crime scene reconstruction by supporting a link between sample donors and actual criminal acts.However,the conventional body fluid identification methods are prone to various limitations,such as time consumption,intensive labor,nonparallel manner,varying degrees of sensitivity and limited specificity.Recently,the analysis of cell-specific messenger RNA expression(mRNA profiling)has been proposed to supplant conventional methods for body fluid identification.Since 2011,the collaborative exercises have been organized by the European DNA Profiling Group(EDNAP)in order to evaluate the robustness and reproducibility of mRNA profiling for body fluid identification.The major advantages of mRNA profiling,compared to the conventional methods,include higher sensitivity,greater specificity,the ability of detecting several body fluids in one multiplex reaction,and compatibility with current DNA extraction and analysis procedure.In the current review,we provided an overview of the present knowledge and detection methodologies of mRNA profiling for forensic body fluid identification and discussed its possible practical application to forensic casework.
文摘In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed
基金This work is supported by National Natural Science Fundation.
文摘The effect of signal sampling depth in SIMS on profile was not reported We have found that the maximum sampling depth in SIMS is considerably bigger than the mean penetration range of primary ions and can skew the profile of secondary ion counts for implanted samples on the more deeper direction from the surface. The effect of maximum sampling depth is true not only for implanted samples but also for the samples in the middle of which there is an other impurity-rich layer. The action principle of signal sampling depth effect and the method of decreasing the error produced by the effect are discussed in this
基金supported in part by the National Natural Science Foundation of China under Grant Nos.62173312 and 61803348in part by the National Major Scientific Instruments Development Project under Grant No.61927807+3 种基金in part by the Program for the Innovative Talents of Higher Education Institutions of ShanxiShanxi Province Science Foundation for Excellent Youthsin part by the Shanxi"1331 Project"Key Subjects Construction(1331KSC)in part by Graduate Innovation Project of Shanxi Province under Grant No.2021Y617。
文摘This article investigates a multi-circular path-following formation control with reinforced transient profiles for nonholonomic vehicles connected by a digraph.A multi-circular formation controller endowed with the feature of spatial-temporal decoupling is devised for a group of vehicles guided by a virtual leader evolving along an implicit path,which allows for a circumnavigation on multiple circles with an anticipant angular spacing.In addition,notice that it typically imposes a stringent time constraint on time-sensitive enclosing scenarios,hence an improved prescribed performance control(IPPC)using novel tighter behavior boundaries is presented to enhance transient capabilities with an ensured appointed-time convergence free from any overshoots.The significant merits are that coordinated circumnavigation along different circles can be realized via executing geometric and dynamic assignments independently with modified transient profiles.Furthermore,all variables existing in the entire system are analyzed to be convergent.Simulation and experimental results are provided to validate the utility of suggested solution.
文摘In the data-driven era of the internet and business environments,constructing accurate user profiles is paramount for personalized user understanding and classification.The traditional TF-IDF algorithm has some limitations when evaluating the impact of words on classification results.Consequently,an improved TF-IDF-K algorithm was introduced in this study,which included an equalization factor,aimed at constructing user profiles by processing and analyzing user search records.Through the training and prediction capabilities of a Support Vector Machine(SVM),it enabled the prediction of user demographic attributes.The experimental results demonstrated that the TF-IDF-K algorithm has achieved a significant improvement in classification accuracy and reliability.
