In response to the rapid increase in world population and subsequent demands for food,edible insects represent an alternative food source for humans that is rich in proteins,amino acids and minerals.Entomophagy is a t...In response to the rapid increase in world population and subsequent demands for food,edible insects represent an alternative food source for humans that is rich in proteins,amino acids and minerals.Entomophagy is a tradition in many countries including China and Thailand,and edible insects have attracted a lot of attention in Western World due to their suitable nutrient composition,high mineral content(e.g.,Fe,Zn,Ca,Mg)and potential use as a supplement in human diet.In this study,we surveyed mineral content in seven insect orders and 67 species of mass produced and wild-harvested edible insects.The total content of essential elements in edible insects was very high in Tenebrio molitor,Bombyx mori,and Zonocerus variegatus.The heavy metal content(summarized for eight species)was below the maximum limit allowed for safe consumption.Sustainable supply of minerals derived from insect biomass is complicated due to the high variations of mineral content in insects and the potential of its change due to processing.展开更多
Annual tree rings are widely recognized as valuable tools for quantifying and reconstructing historical forest disturbances.However,the influence of climate can complicate the detection of disturbance signals,leading ...Annual tree rings are widely recognized as valuable tools for quantifying and reconstructing historical forest disturbances.However,the influence of climate can complicate the detection of disturbance signals,leading to limited accuracy in existing methods.In this study,we propose a random under-sampling boosting(RUB)classifier that integrates both tree-ring and climate variables to enhance the detection of forest insect outbreaks.The study focused on 32 sites in Alberta,Canada,which documented insect outbreaks from 1939 to 2010.Through thorough feature engineering,model development,and tenfold cross-validation,multiple machine learning(ML)models were constructed.These models used ring width indices(RWIs)and climate variables within an 11-year window as input features,with outbreak and non-outbreak occurrences as the corresponding output variables.Our results reveal that the RUB model consistently demonstrated superior overall performance and stability,with an accuracy of 88.1%,which surpassed that of the other ML models.In addition,the relative importance of the feature variables followed the order RWIs>mean maximum temperature(Tmax)from May to July>mean total precipita-tion(Pmean)in July>mean minimum temperature(Tmin)in October.More importantly,the dfoliatR(an R package for detecting insect defoliation)and curve intervention detec-tion methods were inferior to the RUB model.Our findings underscore that integrating tree-ring width and climate vari-ables as predictors in machine learning offers a promising avenue for enhancing the accuracy of detecting forest insect outbreaks.展开更多
Both non-transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] and transgenic ones expressing cowpea trypsin inhibitor were cut at the base of the stem to produce auxoblasts, an...Both non-transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] and transgenic ones expressing cowpea trypsin inhibitor were cut at the base of the stem to produce auxoblasts, and used as source of leaves for insect feeding trials performed on 3 major insect species of poplar, the forest tent caterpillar ( Malacosoma disstria L.), gypsy moth ( Lymantria dispar L.) and willow moth ( Stilpnotia candida Staudinger). The height and basal diameter of trees were measured by the end of that year (2000). The results indicated that the growth elements of transgenic poplars were not interfered by the incorporation of the CpTI gene. Intriguingly, the height and basal diameter of the clone TG04 were much greater than that of the control. The transgenic foliage consumed by insects induced the increase of larval mortality, and decrease of larval wet weight gain, faecal output, pupal weight and egg deposition. Among them 3 transgenic clones, TG04, TG07 and TG71 received special attention for their outstanding insect resistance compared with other transgenic clones, which showed that the CpTI gene in them was expressed more actively and stably than in others.展开更多
Insects respond to changes in microhabitat caused by canopy disturbance, and thus can be used to examine the ecological impacts of harvesting. Single-tree selection harvesting is the most common silvicultural system u...Insects respond to changes in microhabitat caused by canopy disturbance, and thus can be used to examine the ecological impacts of harvesting. Single-tree selection harvesting is the most common silvicultural system used to emulate local small-scale natural disturbance and maintain uneven-aged forest structure in temperate forests. Here, we test for differences in richness, abundance, and composition of hymenopteran and saproxylic insect assemblages at four different taxon levels （selected insect orders; and all hymenopteran families, and braconid subfamilies and morphospecies） between the canopy and understory of unharvested and single-tree selection harvested sites in a northern temperate forest from central Canada. Harvesting had no effect on insect assemblage richness, composition or abundance at the three highest taxon levels （order, family and subfamily）. Similarly, richness and abundance at the lowest-taxon level （braconid morphospecies） were similar, although composition differed slightly between unharvested and harvested stands. Insect assemblages were vertically stratified, with generally higher abundance （for Diptera, Hymenoptera, some hymenopteran families and braconid subfamilies） and richness （for braconid morphospecies） in the understory than the canopy. In particular, composition of the braconid morphospecies assemblage showed relatively low similarity between the understory and canopy. Single-tree selection harvesting appears to influence wood-associated insect taxa only subtly through small changes in community composition at the lowest taxon level, and thus is recommended as a conservative approach for managing these northern temperate forests.展开更多
Insect communities along three elevation gra- dients on Mt. Seongak-san, South Korea, were investigated from May to September 2013 using traps in order to collect basic data for distributional monitoring of species in...Insect communities along three elevation gra- dients on Mt. Seongak-san, South Korea, were investigated from May to September 2013 using traps in order to collect basic data for distributional monitoring of species in a forest ecosystem. A total of 2698 individuals of 309 moth species of 18 families were collected in bucket-light traps, along with 196 individuals of 26 ground-beetle species and 11,276 individuals of 14 ant species in pit-fall traps. The insect community at each site was analyzed using analysisof variance and non-metric multidimensional scaling （NMS）. The ground-beetle and ant abundances varied with elevation. The NMS showed distinct clusters of moths between the ground-beetle and ant distributions with ele- vation and month. The community-level responses of these insect groups to those gradients were analyzed.展开更多
Extensive planting of Bacillus thuringiensis （Bt）-transgenic plants economically benefits society; how-ever, the potential risk they pose is receiving increasing attention. This study used enzyme-linked immunosorben...Extensive planting of Bacillus thuringiensis （Bt）-transgenic plants economically benefits society; how-ever, the potential risk they pose is receiving increasing attention. This study used enzyme-linked immunosorbent assay and fluorescence quantitative PCR （RT-PCR） to monitor the temporal and spatial dynamics of the expression of Bt toxic protein in a forest of 6- to 8-year-old trees of transgenic insect-resistant poplar 741 for three consec- utive years. The enrichment, distribution, and degradation of Bt toxic protein and the influence of transgenic poplars on the targeted insect population, Hyphantria cunea, were investigated. The content of CrylAc toxic protein dynamically changed in transgenic poplar. During the annual growth cycle, the content initially increased, then decreased in the long and the short branches of the crown and in the root system, peaking in August. During the study, the protein did not accumulate overtime. The mRNA transcription of gene CrylAc was almost consistent with the level of the protein, but transcription peaked in July. In the transgenic and control forestland, microscale levels of the CrylAc toxic protein were detected from the soil, but increased accumulation was not observed with the planting year of transgenic poplar. Meanwhile, Bt was isolated and detected molecularly from the soil in the experimental forestland. A systematic investigation of the density of H. cunea in the experimental transgenic poplar forest indi- cated that transgenic Pb29 poplar could resist insects to a certain degree. At peak occurrence of the targeted insects, the density of H. cunea in the experimental forest was significantly lower than in the nontransgenic poplar forest.展开更多
基金founded by Jiangsu Agricultural Science and Technology Innovation Fund(CX(20)3179)Dongminghuanghetan Ecological Agriculture Co.,Ltd(204032897)+1 种基金partially funded funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no.861976,project SUSINCHAINthe German Federal Ministry of Education and Research(BMBF),in the frame of FACCE-SURPLUS/FACCE-JPI project UpWaste,grant number 031B0934A。
文摘In response to the rapid increase in world population and subsequent demands for food,edible insects represent an alternative food source for humans that is rich in proteins,amino acids and minerals.Entomophagy is a tradition in many countries including China and Thailand,and edible insects have attracted a lot of attention in Western World due to their suitable nutrient composition,high mineral content(e.g.,Fe,Zn,Ca,Mg)and potential use as a supplement in human diet.In this study,we surveyed mineral content in seven insect orders and 67 species of mass produced and wild-harvested edible insects.The total content of essential elements in edible insects was very high in Tenebrio molitor,Bombyx mori,and Zonocerus variegatus.The heavy metal content(summarized for eight species)was below the maximum limit allowed for safe consumption.Sustainable supply of minerals derived from insect biomass is complicated due to the high variations of mineral content in insects and the potential of its change due to processing.
基金supported by the Xinjiang Regional Collaborative Innovation Project(2022E01045)Zhejiang University(108000*1942222R1).
文摘Annual tree rings are widely recognized as valuable tools for quantifying and reconstructing historical forest disturbances.However,the influence of climate can complicate the detection of disturbance signals,leading to limited accuracy in existing methods.In this study,we propose a random under-sampling boosting(RUB)classifier that integrates both tree-ring and climate variables to enhance the detection of forest insect outbreaks.The study focused on 32 sites in Alberta,Canada,which documented insect outbreaks from 1939 to 2010.Through thorough feature engineering,model development,and tenfold cross-validation,multiple machine learning(ML)models were constructed.These models used ring width indices(RWIs)and climate variables within an 11-year window as input features,with outbreak and non-outbreak occurrences as the corresponding output variables.Our results reveal that the RUB model consistently demonstrated superior overall performance and stability,with an accuracy of 88.1%,which surpassed that of the other ML models.In addition,the relative importance of the feature variables followed the order RWIs>mean maximum temperature(Tmax)from May to July>mean total precipita-tion(Pmean)in July>mean minimum temperature(Tmin)in October.More importantly,the dfoliatR(an R package for detecting insect defoliation)and curve intervention detec-tion methods were inferior to the RUB model.Our findings underscore that integrating tree-ring width and climate vari-ables as predictors in machine learning offers a promising avenue for enhancing the accuracy of detecting forest insect outbreaks.
文摘Both non-transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] and transgenic ones expressing cowpea trypsin inhibitor were cut at the base of the stem to produce auxoblasts, and used as source of leaves for insect feeding trials performed on 3 major insect species of poplar, the forest tent caterpillar ( Malacosoma disstria L.), gypsy moth ( Lymantria dispar L.) and willow moth ( Stilpnotia candida Staudinger). The height and basal diameter of trees were measured by the end of that year (2000). The results indicated that the growth elements of transgenic poplars were not interfered by the incorporation of the CpTI gene. Intriguingly, the height and basal diameter of the clone TG04 were much greater than that of the control. The transgenic foliage consumed by insects induced the increase of larval mortality, and decrease of larval wet weight gain, faecal output, pupal weight and egg deposition. Among them 3 transgenic clones, TG04, TG07 and TG71 received special attention for their outstanding insect resistance compared with other transgenic clones, which showed that the CpTI gene in them was expressed more actively and stably than in others.
基金funded by the Richard Ivey Foundationthe Haliburton ForestWild Life Reserve
文摘Insects respond to changes in microhabitat caused by canopy disturbance, and thus can be used to examine the ecological impacts of harvesting. Single-tree selection harvesting is the most common silvicultural system used to emulate local small-scale natural disturbance and maintain uneven-aged forest structure in temperate forests. Here, we test for differences in richness, abundance, and composition of hymenopteran and saproxylic insect assemblages at four different taxon levels （selected insect orders; and all hymenopteran families, and braconid subfamilies and morphospecies） between the canopy and understory of unharvested and single-tree selection harvested sites in a northern temperate forest from central Canada. Harvesting had no effect on insect assemblage richness, composition or abundance at the three highest taxon levels （order, family and subfamily）. Similarly, richness and abundance at the lowest-taxon level （braconid morphospecies） were similar, although composition differed slightly between unharvested and harvested stands. Insect assemblages were vertically stratified, with generally higher abundance （for Diptera, Hymenoptera, some hymenopteran families and braconid subfamilies） and richness （for braconid morphospecies） in the understory than the canopy. In particular, composition of the braconid morphospecies assemblage showed relatively low similarity between the understory and canopy. Single-tree selection harvesting appears to influence wood-associated insect taxa only subtly through small changes in community composition at the lowest taxon level, and thus is recommended as a conservative approach for managing these northern temperate forests.
基金supported by ‘‘Study on impacts of the climate changes on climate-sensitive insects in forests’’(Project No.KNA-1-2-11,11-3) of Korea National Arboretum
文摘Insect communities along three elevation gra- dients on Mt. Seongak-san, South Korea, were investigated from May to September 2013 using traps in order to collect basic data for distributional monitoring of species in a forest ecosystem. A total of 2698 individuals of 309 moth species of 18 families were collected in bucket-light traps, along with 196 individuals of 26 ground-beetle species and 11,276 individuals of 14 ant species in pit-fall traps. The insect community at each site was analyzed using analysisof variance and non-metric multidimensional scaling （NMS）. The ground-beetle and ant abundances varied with elevation. The NMS showed distinct clusters of moths between the ground-beetle and ant distributions with ele- vation and month. The community-level responses of these insect groups to those gradients were analyzed.
基金supported by the National High Technology Research and Development Program of China(863 Program)(Grant No.2013AA102703)
文摘Extensive planting of Bacillus thuringiensis （Bt）-transgenic plants economically benefits society; how-ever, the potential risk they pose is receiving increasing attention. This study used enzyme-linked immunosorbent assay and fluorescence quantitative PCR （RT-PCR） to monitor the temporal and spatial dynamics of the expression of Bt toxic protein in a forest of 6- to 8-year-old trees of transgenic insect-resistant poplar 741 for three consec- utive years. The enrichment, distribution, and degradation of Bt toxic protein and the influence of transgenic poplars on the targeted insect population, Hyphantria cunea, were investigated. The content of CrylAc toxic protein dynamically changed in transgenic poplar. During the annual growth cycle, the content initially increased, then decreased in the long and the short branches of the crown and in the root system, peaking in August. During the study, the protein did not accumulate overtime. The mRNA transcription of gene CrylAc was almost consistent with the level of the protein, but transcription peaked in July. In the transgenic and control forestland, microscale levels of the CrylAc toxic protein were detected from the soil, but increased accumulation was not observed with the planting year of transgenic poplar. Meanwhile, Bt was isolated and detected molecularly from the soil in the experimental forestland. A systematic investigation of the density of H. cunea in the experimental transgenic poplar forest indi- cated that transgenic Pb29 poplar could resist insects to a certain degree. At peak occurrence of the targeted insects, the density of H. cunea in the experimental forest was significantly lower than in the nontransgenic poplar forest.
