Due to technical limitations,existing vibration isolation and energy harvesting(VIEH)devices have poor performance at low frequency.This paper proposes a new multilink-spring mechanism(MLSM)that can be used to solve t...Due to technical limitations,existing vibration isolation and energy harvesting(VIEH)devices have poor performance at low frequency.This paper proposes a new multilink-spring mechanism(MLSM)that can be used to solve this problem.The VIEH performance of the MLSM under harmonic excitation and Gaussian white noise was analyzed.It was found that the MLSM has good vibration isolation performance for low-frequency isolation and the frequency band can be widened by adjusting parameters to achieve a higher energy harvesting power.By comparison with two special cases,the results show that the MLSM is basically the same as the other two oscillators in terms of vibration isolation but has better energy harvesting performance under multistable characteristics.The MLSM is expected to reduce the impact of vibration on high-precision sensitive equipment in some special sites such as subways and mines,and at the same time supply power to structural health monitoring devices.展开更多
Optical isolators,the photonic analogs of electronic diodes,are essential for ensuring the unidirectional flow of light in optical systems,thereby mitigating the destabilizing effects of back reflections.Thin-film lit...Optical isolators,the photonic analogs of electronic diodes,are essential for ensuring the unidirectional flow of light in optical systems,thereby mitigating the destabilizing effects of back reflections.Thin-film lithium niobate(TFLN),hailed as“the silicon of photonics,”has emerged as a pivotal material in the realm of chip-scale nonlinear optics,propelling the demand for compact optical isolators.We report a breakthrough in the development of a fully passive,integrated optical isolator on the TFLN platform,leveraging the Kerr effect to achieve an impressive 10.3 dB of isolation with a minimal insertion loss of 1.87 dB.Further theoretical simulations have demonstrated that our design,when applied to a microring resonator with a Q factor of 5×10^(6),can achieve 20 dB of isolation with an input power of merely 8 mW.This advancement underscores the immense potential of lithium niobate-based Kerr-effect isolators in propelling the integration and application of high-performance on-chip lasers,heralding a new era in integrated photonics.展开更多
A modified guanidinium isothiocyanate method was used to extract total RNA from two forest insect species Clostera anastomosis and Saperda populnea. The integrity of RNA was demonstrated by the methods of gel electrop...A modified guanidinium isothiocyanate method was used to extract total RNA from two forest insect species Clostera anastomosis and Saperda populnea. The integrity of RNA was demonstrated by the methods of gel electrophoresis and cDNA analysis. Typical A260/ A280 absorbance ratio of the total RNA was in range of 1.8 to 2.0. The size of double strand cDNAs obtained by RT-PCR was more than 2 kb, which indicated that intact mRNA was obtained. The fragments of β-actin and chitinase gene from the RNA of C. anastomosis were obtained by RT-PCR, which indicated that the RNA could be used for other molecular operation. By this procedure, RNAs could be extracted and analyzed by electrophoresis from at least 8 samples within 4 hours. These results showed that this method was time- and cost-saving and effective.展开更多
The relation of mass, stiffness and rate of damping is obtained by using the mechanical analysis of the obstructive vibration system of two dimensions for the design of the obstructive vibration system of more freedom...The relation of mass, stiffness and rate of damping is obtained by using the mechanical analysis of the obstructive vibration system of two dimensions for the design of the obstructive vibration system of more freedom and the micro vibration test bed. The result of stimulational experiment indicates that the isolation of vibration of this system is satisfactory. The design method of vibration can be used as the reference to ultra precision machine tool, super micro orientation machanism and so on.展开更多
A direct synthesis of methyl levulinate from cellulose alcoholysis in methanol medium under mild condition(180 210 C)catalyzed by extremely low concentration sulfuric acid(0.01 mol/L)and the product isolation were dev...A direct synthesis of methyl levulinate from cellulose alcoholysis in methanol medium under mild condition(180 210 C)catalyzed by extremely low concentration sulfuric acid(0.01 mol/L)and the product isolation were developed in this study.Effects of different process variables towards the catalytic performance were performed as a function of reaction time.The results indicated that sulfuric acid concentration,temperature and initial cellulose concentration had significant effects on the synthesis of methyl levulinate.An optimized yield of around 50%was achieved at 210 C for 120 min with sulfuric acid concentration of 0.01 mol/L and initial cellulose concentration below 100 g/L.The resulting product mixture was isolated by a distillation technique that combines an atmospheric distillation with a vacuum distillation where n-dodecane was added to help distill the heavy fraction.The light fraction including mainly methanol could be reused as the reaction medium without any substantial change in the yield of methyl levulinate.The chemical composition and structural of lower heavy fraction were characterized by GC/MS,FTIR,1H-NMR and13C-NMR techniques.Methyl levulinate was found to be a major ingredient of lower heavy fraction with the content over 96%.This pathway is efficient,environmentally benign and economical for the production of pure levulinate esters from cellulose.展开更多
A remote-control tether-less isolation tool is a mechanical device that is normally used in pipelines to block the flow at a given position by transforming a blocking module. In this study, the interactions between th...A remote-control tether-less isolation tool is a mechanical device that is normally used in pipelines to block the flow at a given position by transforming a blocking module. In this study, the interactions between the fluid and the plug module of the isolation tool were investigated. Simulations of the plug process and particle image velocimetry measurements were performed to study the flow characteristics. Numerical solutions for the continuity, momentum, and energy equations were obtained by using commercial software based on finite-volume techniques. Box–Behnken design was applied, and response surface methodology（RSM）-based CFD simulation analysis was conducted. The dynamic model in the plug process was built by RSM and used to evaluate the influences of the main mechanical parameters on the pressure during the plug process. The diameter of the isolation tool and the diameter of the plug module have strong influences on the process, and the length of the isolation tool has only a little influence on the plug process.展开更多
Adopting a soft site model built on soft interlayer soil foundation,a shaking table test for soft interlayer soil-isolated structure interaction is conducted to investigate the seismic response of isolated structure o...Adopting a soft site model built on soft interlayer soil foundation,a shaking table test for soft interlayer soil-isolated structure interaction is conducted to investigate the seismic response of isolated structure on soft site,and analyze its isolation effect.Test results show that the test can reflect the earthquake response characteristics of isolated structure on soft site.It is on soft site that the dynamic characteristics of isolated structure,acceleration magnification factor(AMF)of isolated structure and isolation efficiency of the isolation layer differ from those on rigid foundation with an soil-structure interaction(SSI)effect,represented by the reduction in fundamental vibration frequency of isolated structure and the increase of damping ratio with changes of the SSI effect.SSI can either increase or decrease AMF of isolated structure on soft site,depending on the characteristics of earthquake motion input.Furthermore,the isolation efficiency of isolation layer on soft site is decreased with the SSI effect,which is related to the peak ground acceleration(PGA)and the characteristics of earthquake motion input.展开更多
Total RNA was isolated from shoots regenerated in vitro of Populus suaveolens by the modified method of CTAB, and two clear bands of rRNA (28S and 18S) were observed in agarose electrophoresis. In addition, the values...Total RNA was isolated from shoots regenerated in vitro of Populus suaveolens by the modified method of CTAB, and two clear bands of rRNA (28S and 18S) were observed in agarose electrophoresis. In addition, the values of OD260/OD280 and OD260/OD230 of extracted RNA were 2.12 and 2.23 respectively. The results show that RNA is little decomposed and the purity of RNA is high. Moreover, RNA isolated by the modified method of CTAB reagent had been successfully used for reverse transcription of P. suaveolens cDNAs and ideal special band was observed.展开更多
We used callus of Populus euphratica Olive to isolate protoplasts, and IT fluxes across plasma membrane were investigated. The concentration of enzymes for protoplast isolation, e.g. cellulase, pectolyase, macerozyme,...We used callus of Populus euphratica Olive to isolate protoplasts, and IT fluxes across plasma membrane were investigated. The concentration of enzymes for protoplast isolation, e.g. cellulase, pectolyase, macerozyme, hemicellulase, and sorbitol content, incubation time were systemically studied. High yield and viability of protoplast was achieved after 6-8 hours incubation of P. euphratica callus in enzyme solution containing 1.5% （w：v） cellulase R-10, 0.1% （w：v） pectolyase Y-23, 0.2% （w：v） macerozyme R-10, 0.05% （w：v） hemicellulase and 0.75M）.80 mol·L^-1 sorbitol. Non-invasively ion selective microelectrode technique was used to access proton fluxes in the absence and presence of NaCl （20 mmol.L-1）. Salt-induced transient net IT effiux was observed in the plasma membrane ofP. euphratica cells. The shift of IT flux response to NaC1 shock and the relevance to salt tolerance were discussed.展开更多
This paper is devoted to develop an expert system to manage the fault isolation and maintenance knowledge of the engine indication and crew alerting system (EICAS). The object oriented programming (OOP) technique and...This paper is devoted to develop an expert system to manage the fault isolation and maintenance knowledge of the engine indication and crew alerting system (EICAS). The object oriented programming (OOP) technique and the microsoft foundation class (MFC) are applied to set up a frame decision tree (FDT) which incorporates the expert system′s knowledge base, inference engine and user interface. Once a fault symptom indicated by the EICAS is input, by inferring step by step, the expert system can locate it in the engine and provide some homologous constructive maintenance advice.展开更多
we investigate the effects of 60^Co γ-ray irradiation on the 130 nm partially-depleted silicon-on-isolator （PDSOI） input/output （I/O） n-MOSFETs. A shallow trench isolation （STI） parasitic transistor is responsi...we investigate the effects of 60^Co γ-ray irradiation on the 130 nm partially-depleted silicon-on-isolator （PDSOI） input/output （I/O） n-MOSFETs. A shallow trench isolation （STI） parasitic transistor is responsible for the observed hump in the back-gate transfer characteristic curve. The STI parasitic transistor, in which the trench oxide acts as the gate oxide, is sensitive to the radiation, and it introduces a new way to characterize the total ionizing dose （TID） responses in the STI oxide. A radiation enhanced drain induced barrier lower （DIBL） effect is observed in the STI parasitic transistor. It is manifested as the drain bias dependence of the radiation-induced off-state leakage and the increase of the DIBL parameter in the STI parasitic transistor after irradiation. Increasing the doping concentration in the whole body region or just near the STI sidewall can increase the threshold voltage of the STI parasitic transistor, and further reduce the radiation-induced off-state leakage. Moreover, we find that the radiation-induced trapped charge in the buried oxide leads to an obvious front-gate threshold voltage shift through the coupling effect. The high doping concentration in the body can effectively suppress the radiation-induced coupling effect.展开更多
Background Atrial fibrillation(AF)is a generally acknowledged turning-point of the natural history of hypertrophic cardiomyopathy(HCM);however,data from the cryoballoon ablation(CBA)for AF in HCM patients are relative...Background Atrial fibrillation(AF)is a generally acknowledged turning-point of the natural history of hypertrophic cardiomyopathy(HCM);however,data from the cryoballoon ablation(CBA)for AF in HCM patients are relatively scarce.The study aimed to evaluate the efficacy and safety of CBA in HCM patients with AF.Methods We retrospectively analyzed HCM patients among 1253 patients with symptomatic AF who underwent CBA for pulmonary vein isolation in a single center.The study analyzed the AF recurrence and assessed the CBA indexes,including nadir temperature,time-to-isolation,CBA failure,pulmonary vein potentials(PVPs),and redo procedure.Results A total of 108 patients were included(mean age:59.0±6.9 years),27 patients(25%)had HCM,with the median follow-up duration of 25.5 months.The one-year AF-free rates were 79.0%vs.63.0%(non-HCM vs.HCM),while the two-year AF-free rates were 77.8%vs.55.1%[hazard ratio(HR)=2.758,log-rank P=0.024].Patients with persistent AF had poor AF-free rates compared to those with paroxysmal AF(P<0.001).The CBA failure was the most common in the right inferior pulmonary veins,which had the lowest PVPs.Multivariate Cox regression analysis indicated that HCM and persistent AF were risk factors for AF recurrence(HR=2.74,95%CI:1.29–5.79,P=0.008;and HR=3.97,95%CI:1.85–8.54,P<0.001,respectively).Conclusions The CBA can be effectively and safely used to treat HCM patients with symptomatic AF.The freedom from AF for HCM patients after CBA is relatively low compared to that for non-HCM patients.展开更多
Rigid blocking masses are located in the typical base structure of a power cabin based on the impedance mismatch principle.By combining the acoustic-structural coupling method and statistical energy analysis,the full-...Rigid blocking masses are located in the typical base structure of a power cabin based on the impedance mismatch principle.By combining the acoustic-structural coupling method and statistical energy analysis,the full-band vibration and sound radiation reduction effect of vibration isolation masses located in a base structure was researched.The influence of the blocking mass’ cross-section size and shape parameters and the layout location of the base isolation performance was discussed.Furthermore,the effectiveness of rigid vibration isolation design of the base structure was validated.The results show that the medium and high frequency vibration and sound radiation of a power cabin are effectively reduced by a blocking mass.Concerning weight increment and section requirement,suitably increasing the blocking mass size and section height and reducing section width can result in an efficiency-cost ratio.展开更多
Objective: To isolate neural progenitors from midbrain of new born rats. Methods: Cell groups with cloning capability were obtained from midbrain of new-born rats by using serum-free and floating cell culture. Immunoc...Objective: To isolate neural progenitors from midbrain of new born rats. Methods: Cell groups with cloning capability were obtained from midbrain of new-born rats by using serum-free and floating cell culture. Immunocytochemistry was applied to detect antigens such as BrdU, Nestin and specific markers for mature neural cells. Results: Cell groups isolated from midbrain propagated in succession and shaped 'neuro-spheres'. Nestin antigen was expressed in the course of proliferation. After differentiation, epitopes of neuron , astrocyte and oligodendrocyte were all found in the neural progenitors-derived cells, the most majority were astrocyte-epitoped cells. Conclusion: Cell groups expressing Nestin isolated from midbrain of new-born rats have properties of propagating, multi-potency differentiating and self-renewal. It is demonstrated that the cell groups are stem-like neural progenitors in the central nervous system, which are qualified for cell therapy of diseases in central nervous system.展开更多
During the pipeline plugging process,both the pipeline and the pipe isolation tool(PIT)will be greatly damaged,due to the violent vibration of the flow field.In this study,it was proposed for the first time to reduce ...During the pipeline plugging process,both the pipeline and the pipe isolation tool(PIT)will be greatly damaged,due to the violent vibration of the flow field.In this study,it was proposed for the first time to reduce the vibration of the flow field during the plugging process by optimizing the surface structure of the PIT.Firstly,the central composite design(CCD)was used to obtain the optimization schemes,and the drag coefficient and pressure coefficient were proposed to evaluate the degree of flow field changes.Secondly,a series of computational fluid dynamics(CFD)simulations were performed to obtain the drag coefficient and pressure coefficient during dynamic plugging.And the mathematical model of drag coefficient and pressure coefficient with the surface structure of the PIT were established respectively.Then,a modified particle swarm optimization(PSO)was applied to predict the optimal value of the surface structure of the PIT.Finally,an experimental rig was built to verify the effectiveness of the optimization.The results showed that the improved method could reduce the flow field vibration by 49.56%.This study provides a reference for the design of the PIT surface structure for flow field vibration technology.展开更多
This paper introduces a design of high-precision high-voltage fiber-optic analog sig-nal isolation converter based on the technology of Voltage-to-Frequency (V/F) and Frequency-to-Voltage (F/V) conversion. It describe...This paper introduces a design of high-precision high-voltage fiber-optic analog sig-nal isolation converter based on the technology of Voltage-to-Frequency (V/F) and Frequency-to-Voltage (F/V) conversion. It describes the principle, system configuration and hardware design.展开更多
In considering the theory of structural dynamic optimization design, a design method of the structural style of ship composite brace with rigid vibration isolation mass was studied. Two kinds of structural dynamic opt...In considering the theory of structural dynamic optimization design, a design method of the structural style of ship composite brace with rigid vibration isolation mass was studied. Two kinds of structural dynamic optimization formulations minimizing the vibration acceleration of the non-pressure hull on the restraining condition of the gross weight of the ship cabin were established: 1) dynamic optimization of the sectional dimensions of the rigid vibration isolation mass in the composite brace; 2) dynamic optimization of the arranging position of the rigid vibration isolation mass. Through the optimization results, sectional dimensions and the arranging position of the rigid vibration isolation mass with better performance in reducing vibration were gained, and some reference was provided for practical engineering designs as well as enrichment of the design method of a novel ship vibration-isolation brace.展开更多
We present a study on the single event transient （SET） induced by a pulsed laser in different silicon-germanium （SiGe） heterojunction bipolar transistors （HBTs） with the structure of local oxidation of silicon ...We present a study on the single event transient （SET） induced by a pulsed laser in different silicon-germanium （SiGe） heterojunction bipolar transistors （HBTs） with the structure of local oxidation of silicon （LOCOS） and deep trench isolation （DTI）. The experimental results are discussed in detail and it is demonstrated that a SiGe HBT with the structure of LOCOS is more sensitive than the DTI SiGe HBT in the SET. Because of the limitation of the DTI structure, the charge collection of diffusion in the DTI SiGe HBT is less than that of the LOCOS SiGe HBT. The SET sensitive area of the LOCOS SiGe HBT is located in the eollector-substrate （C/S） junction, while the sensitive area of the DTI SiGe HBT is located near to the collector electrodes.展开更多
基金Project supported by Guangdong Basic and Applied Basic Research Foundation(Grant Nos.2022A1515010967 and 2023A1515012821)the National Natural Science Foundation of China(Grant Nos.12002272 and 12272293)Opening Project of Applied Mechanics and Structure Safety Key Laboratory of Sichuan Province(Grant No.SZDKF-202101)。
文摘Due to technical limitations,existing vibration isolation and energy harvesting(VIEH)devices have poor performance at low frequency.This paper proposes a new multilink-spring mechanism(MLSM)that can be used to solve this problem.The VIEH performance of the MLSM under harmonic excitation and Gaussian white noise was analyzed.It was found that the MLSM has good vibration isolation performance for low-frequency isolation and the frequency band can be widened by adjusting parameters to achieve a higher energy harvesting power.By comparison with two special cases,the results show that the MLSM is basically the same as the other two oscillators in terms of vibration isolation but has better energy harvesting performance under multistable characteristics.The MLSM is expected to reduce the impact of vibration on high-precision sensitive equipment in some special sites such as subways and mines,and at the same time supply power to structural health monitoring devices.
基金Project supported by the National Key Research and Development Program of China(Grant Nos.2022YFF0712800 and 2019YFA0308700)。
文摘Optical isolators,the photonic analogs of electronic diodes,are essential for ensuring the unidirectional flow of light in optical systems,thereby mitigating the destabilizing effects of back reflections.Thin-film lithium niobate(TFLN),hailed as“the silicon of photonics,”has emerged as a pivotal material in the realm of chip-scale nonlinear optics,propelling the demand for compact optical isolators.We report a breakthrough in the development of a fully passive,integrated optical isolator on the TFLN platform,leveraging the Kerr effect to achieve an impressive 10.3 dB of isolation with a minimal insertion loss of 1.87 dB.Further theoretical simulations have demonstrated that our design,when applied to a microring resonator with a Q factor of 5×10^(6),can achieve 20 dB of isolation with an input power of merely 8 mW.This advancement underscores the immense potential of lithium niobate-based Kerr-effect isolators in propelling the integration and application of high-performance on-chip lasers,heralding a new era in integrated photonics.
基金This study was supported by Key Project of Harbin City (2005AA6CN074)
文摘A modified guanidinium isothiocyanate method was used to extract total RNA from two forest insect species Clostera anastomosis and Saperda populnea. The integrity of RNA was demonstrated by the methods of gel electrophoresis and cDNA analysis. Typical A260/ A280 absorbance ratio of the total RNA was in range of 1.8 to 2.0. The size of double strand cDNAs obtained by RT-PCR was more than 2 kb, which indicated that intact mRNA was obtained. The fragments of β-actin and chitinase gene from the RNA of C. anastomosis were obtained by RT-PCR, which indicated that the RNA could be used for other molecular operation. By this procedure, RNAs could be extracted and analyzed by electrophoresis from at least 8 samples within 4 hours. These results showed that this method was time- and cost-saving and effective.
文摘The relation of mass, stiffness and rate of damping is obtained by using the mechanical analysis of the obstructive vibration system of two dimensions for the design of the obstructive vibration system of more freedom and the micro vibration test bed. The result of stimulational experiment indicates that the isolation of vibration of this system is satisfactory. The design method of vibration can be used as the reference to ultra precision machine tool, super micro orientation machanism and so on.
基金supported by the National Key Basic Research Program (2010CB732201) from the Ministry of Science and Technology of Chinathe State Key Laboratory Open Foundation of Pulp and Paper Engineering of China (201225)
文摘A direct synthesis of methyl levulinate from cellulose alcoholysis in methanol medium under mild condition(180 210 C)catalyzed by extremely low concentration sulfuric acid(0.01 mol/L)and the product isolation were developed in this study.Effects of different process variables towards the catalytic performance were performed as a function of reaction time.The results indicated that sulfuric acid concentration,temperature and initial cellulose concentration had significant effects on the synthesis of methyl levulinate.An optimized yield of around 50%was achieved at 210 C for 120 min with sulfuric acid concentration of 0.01 mol/L and initial cellulose concentration below 100 g/L.The resulting product mixture was isolated by a distillation technique that combines an atmospheric distillation with a vacuum distillation where n-dodecane was added to help distill the heavy fraction.The light fraction including mainly methanol could be reused as the reaction medium without any substantial change in the yield of methyl levulinate.The chemical composition and structural of lower heavy fraction were characterized by GC/MS,FTIR,1H-NMR and13C-NMR techniques.Methyl levulinate was found to be a major ingredient of lower heavy fraction with the content over 96%.This pathway is efficient,environmentally benign and economical for the production of pure levulinate esters from cellulose.
基金supported by the National Natural Science Foundation of China (Grant No. 51575528)the Scientific Research Foundation of the Education Ministry for Returned Chinese Scholars (China)+1 种基金the State Key Laboratory for Coal Resources and Safe Mining, China University of Mining and Technology (No. SKLCRSM10KFB04)the Science Foundation of China University of Petroleum, Beijing (No. YXQN-2014-02)
文摘A remote-control tether-less isolation tool is a mechanical device that is normally used in pipelines to block the flow at a given position by transforming a blocking module. In this study, the interactions between the fluid and the plug module of the isolation tool were investigated. Simulations of the plug process and particle image velocimetry measurements were performed to study the flow characteristics. Numerical solutions for the continuity, momentum, and energy equations were obtained by using commercial software based on finite-volume techniques. Box–Behnken design was applied, and response surface methodology（RSM）-based CFD simulation analysis was conducted. The dynamic model in the plug process was built by RSM and used to evaluate the influences of the main mechanical parameters on the pressure during the plug process. The diameter of the isolation tool and the diameter of the plug module have strong influences on the process, and the length of the isolation tool has only a little influence on the plug process.
基金supported by the Jiangsu Natural Science Foundation of China(Grant No.BK2012477)the Science Research Foundation of Nanjing Institute of Technology(CKJA201505,JCYJ201618)
文摘Adopting a soft site model built on soft interlayer soil foundation,a shaking table test for soft interlayer soil-isolated structure interaction is conducted to investigate the seismic response of isolated structure on soft site,and analyze its isolation effect.Test results show that the test can reflect the earthquake response characteristics of isolated structure on soft site.It is on soft site that the dynamic characteristics of isolated structure,acceleration magnification factor(AMF)of isolated structure and isolation efficiency of the isolation layer differ from those on rigid foundation with an soil-structure interaction(SSI)effect,represented by the reduction in fundamental vibration frequency of isolated structure and the increase of damping ratio with changes of the SSI effect.SSI can either increase or decrease AMF of isolated structure on soft site,depending on the characteristics of earthquake motion input.Furthermore,the isolation efficiency of isolation layer on soft site is decreased with the SSI effect,which is related to the peak ground acceleration(PGA)and the characteristics of earthquake motion input.
基金Supported by the National Natural Science Foundation of China (Grant No. 30271093)
文摘Total RNA was isolated from shoots regenerated in vitro of Populus suaveolens by the modified method of CTAB, and two clear bands of rRNA (28S and 18S) were observed in agarose electrophoresis. In addition, the values of OD260/OD280 and OD260/OD230 of extracted RNA were 2.12 and 2.23 respectively. The results show that RNA is little decomposed and the purity of RNA is high. Moreover, RNA isolated by the modified method of CTAB reagent had been successfully used for reverse transcription of P. suaveolens cDNAs and ideal special band was observed.
基金the key project of National Natural Science Foundation of China （30430430） the HI-TECH Research and Development Program of China （863 Program, 2006AA10Z131）+1 种基金 a Foundation for the Author of National Excellent Doctoral Dissertation of PR China （200152） the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institution of MOE, PRC （2002-323）.
文摘We used callus of Populus euphratica Olive to isolate protoplasts, and IT fluxes across plasma membrane were investigated. The concentration of enzymes for protoplast isolation, e.g. cellulase, pectolyase, macerozyme, hemicellulase, and sorbitol content, incubation time were systemically studied. High yield and viability of protoplast was achieved after 6-8 hours incubation of P. euphratica callus in enzyme solution containing 1.5% （w：v） cellulase R-10, 0.1% （w：v） pectolyase Y-23, 0.2% （w：v） macerozyme R-10, 0.05% （w：v） hemicellulase and 0.75M）.80 mol·L^-1 sorbitol. Non-invasively ion selective microelectrode technique was used to access proton fluxes in the absence and presence of NaCl （20 mmol.L-1）. Salt-induced transient net IT effiux was observed in the plasma membrane ofP. euphratica cells. The shift of IT flux response to NaC1 shock and the relevance to salt tolerance were discussed.
文摘This paper is devoted to develop an expert system to manage the fault isolation and maintenance knowledge of the engine indication and crew alerting system (EICAS). The object oriented programming (OOP) technique and the microsoft foundation class (MFC) are applied to set up a frame decision tree (FDT) which incorporates the expert system′s knowledge base, inference engine and user interface. Once a fault symptom indicated by the EICAS is input, by inferring step by step, the expert system can locate it in the engine and provide some homologous constructive maintenance advice.
基金supported by the Opening Project of Science and Technology on Reliability Physics and Application Technology of Electronic Component Laboratory,China(Grant No.ZHD201205)the National Natural Science Foundation of China(Grant No.61106103)
文摘we investigate the effects of 60^Co γ-ray irradiation on the 130 nm partially-depleted silicon-on-isolator （PDSOI） input/output （I/O） n-MOSFETs. A shallow trench isolation （STI） parasitic transistor is responsible for the observed hump in the back-gate transfer characteristic curve. The STI parasitic transistor, in which the trench oxide acts as the gate oxide, is sensitive to the radiation, and it introduces a new way to characterize the total ionizing dose （TID） responses in the STI oxide. A radiation enhanced drain induced barrier lower （DIBL） effect is observed in the STI parasitic transistor. It is manifested as the drain bias dependence of the radiation-induced off-state leakage and the increase of the DIBL parameter in the STI parasitic transistor after irradiation. Increasing the doping concentration in the whole body region or just near the STI sidewall can increase the threshold voltage of the STI parasitic transistor, and further reduce the radiation-induced off-state leakage. Moreover, we find that the radiation-induced trapped charge in the buried oxide leads to an obvious front-gate threshold voltage shift through the coupling effect. The high doping concentration in the body can effectively suppress the radiation-induced coupling effect.
基金supported by the National Natural Science Foundation of China(No.81670309)。
文摘Background Atrial fibrillation(AF)is a generally acknowledged turning-point of the natural history of hypertrophic cardiomyopathy(HCM);however,data from the cryoballoon ablation(CBA)for AF in HCM patients are relatively scarce.The study aimed to evaluate the efficacy and safety of CBA in HCM patients with AF.Methods We retrospectively analyzed HCM patients among 1253 patients with symptomatic AF who underwent CBA for pulmonary vein isolation in a single center.The study analyzed the AF recurrence and assessed the CBA indexes,including nadir temperature,time-to-isolation,CBA failure,pulmonary vein potentials(PVPs),and redo procedure.Results A total of 108 patients were included(mean age:59.0±6.9 years),27 patients(25%)had HCM,with the median follow-up duration of 25.5 months.The one-year AF-free rates were 79.0%vs.63.0%(non-HCM vs.HCM),while the two-year AF-free rates were 77.8%vs.55.1%[hazard ratio(HR)=2.758,log-rank P=0.024].Patients with persistent AF had poor AF-free rates compared to those with paroxysmal AF(P<0.001).The CBA failure was the most common in the right inferior pulmonary veins,which had the lowest PVPs.Multivariate Cox regression analysis indicated that HCM and persistent AF were risk factors for AF recurrence(HR=2.74,95%CI:1.29–5.79,P=0.008;and HR=3.97,95%CI:1.85–8.54,P<0.001,respectively).Conclusions The CBA can be effectively and safely used to treat HCM patients with symptomatic AF.The freedom from AF for HCM patients after CBA is relatively low compared to that for non-HCM patients.
基金Supported by the International Cooperation Program under Grant No.2007DFR80340the National Natural Science Foundation of China under Grant No.50779007
文摘Rigid blocking masses are located in the typical base structure of a power cabin based on the impedance mismatch principle.By combining the acoustic-structural coupling method and statistical energy analysis,the full-band vibration and sound radiation reduction effect of vibration isolation masses located in a base structure was researched.The influence of the blocking mass’ cross-section size and shape parameters and the layout location of the base isolation performance was discussed.Furthermore,the effectiveness of rigid vibration isolation design of the base structure was validated.The results show that the medium and high frequency vibration and sound radiation of a power cabin are effectively reduced by a blocking mass.Concerning weight increment and section requirement,suitably increasing the blocking mass size and section height and reducing section width can result in an efficiency-cost ratio.
基金National Natural Science Foundation of China (No. 30170469)
文摘Objective: To isolate neural progenitors from midbrain of new born rats. Methods: Cell groups with cloning capability were obtained from midbrain of new-born rats by using serum-free and floating cell culture. Immunocytochemistry was applied to detect antigens such as BrdU, Nestin and specific markers for mature neural cells. Results: Cell groups isolated from midbrain propagated in succession and shaped 'neuro-spheres'. Nestin antigen was expressed in the course of proliferation. After differentiation, epitopes of neuron , astrocyte and oligodendrocyte were all found in the neural progenitors-derived cells, the most majority were astrocyte-epitoped cells. Conclusion: Cell groups expressing Nestin isolated from midbrain of new-born rats have properties of propagating, multi-potency differentiating and self-renewal. It is demonstrated that the cell groups are stem-like neural progenitors in the central nervous system, which are qualified for cell therapy of diseases in central nervous system.
基金financially supported by the National Natural Science Foundation of China(Grant No.51575528)。
文摘During the pipeline plugging process,both the pipeline and the pipe isolation tool(PIT)will be greatly damaged,due to the violent vibration of the flow field.In this study,it was proposed for the first time to reduce the vibration of the flow field during the plugging process by optimizing the surface structure of the PIT.Firstly,the central composite design(CCD)was used to obtain the optimization schemes,and the drag coefficient and pressure coefficient were proposed to evaluate the degree of flow field changes.Secondly,a series of computational fluid dynamics(CFD)simulations were performed to obtain the drag coefficient and pressure coefficient during dynamic plugging.And the mathematical model of drag coefficient and pressure coefficient with the surface structure of the PIT were established respectively.Then,a modified particle swarm optimization(PSO)was applied to predict the optimal value of the surface structure of the PIT.Finally,an experimental rig was built to verify the effectiveness of the optimization.The results showed that the improved method could reduce the flow field vibration by 49.56%.This study provides a reference for the design of the PIT surface structure for flow field vibration technology.
基金This work was supported by the National Meg-Science Engineering Project of the Chinese Government.
文摘This paper introduces a design of high-precision high-voltage fiber-optic analog sig-nal isolation converter based on the technology of Voltage-to-Frequency (V/F) and Frequency-to-Voltage (F/V) conversion. It describes the principle, system configuration and hardware design.
基金Supported by the Shipbuilding Industry of National Defense Science and Technology Research Projects in Advance (153010110031)
文摘In considering the theory of structural dynamic optimization design, a design method of the structural style of ship composite brace with rigid vibration isolation mass was studied. Two kinds of structural dynamic optimization formulations minimizing the vibration acceleration of the non-pressure hull on the restraining condition of the gross weight of the ship cabin were established: 1) dynamic optimization of the sectional dimensions of the rigid vibration isolation mass in the composite brace; 2) dynamic optimization of the arranging position of the rigid vibration isolation mass. Through the optimization results, sectional dimensions and the arranging position of the rigid vibration isolation mass with better performance in reducing vibration were gained, and some reference was provided for practical engineering designs as well as enrichment of the design method of a novel ship vibration-isolation brace.
基金Supported by the National Natural Science Foundation of China under Grant Nos 61274106
文摘We present a study on the single event transient （SET） induced by a pulsed laser in different silicon-germanium （SiGe） heterojunction bipolar transistors （HBTs） with the structure of local oxidation of silicon （LOCOS） and deep trench isolation （DTI）. The experimental results are discussed in detail and it is demonstrated that a SiGe HBT with the structure of LOCOS is more sensitive than the DTI SiGe HBT in the SET. Because of the limitation of the DTI structure, the charge collection of diffusion in the DTI SiGe HBT is less than that of the LOCOS SiGe HBT. The SET sensitive area of the LOCOS SiGe HBT is located in the eollector-substrate （C/S） junction, while the sensitive area of the DTI SiGe HBT is located near to the collector electrodes.
