Biosensors, which are the products of the biotechnology industry, are among the key projects of the 7th, 8th, and 9th Fiveyear Plans of China Science ＆ Technology Developing Programs, respectively, and they play an i...Biosensors, which are the products of the biotechnology industry, are among the key projects of the 7th, 8th, and 9th Fiveyear Plans of China Science ＆ Technology Developing Programs, respectively, and they play an important role in developing and reforming traditional biotechnology. SBA series biosensor analyzer, as the only one commercial biosensor in China, has attracted lots of attention in the process of information gathering and measurement for biological industry with the development of technology and society. In this paper, we presented an overview of the most important contributions dealing with the monitoring of the biochemical analytes in fermentation processes using SBA series biosensor analyzers in China. Future trends of the biosensor analyzer in China were also mentioned in the last section.展开更多
This paper theoretically studies the continuous immobilized cell (IMC) fer mentation system. A kinetic model of IMC is established, and the relational expressions between production rate, substrate concentration, biom...This paper theoretically studies the continuous immobilized cell (IMC) fer mentation system. A kinetic model of IMC is established, and the relational expressions between production rate, substrate concentration, biomass concentration and dilution rate in the IMC continuous stirred tank reactor (CSTR) are derived. These equations and some numerical calculations show that as compared with the free cell system the IMC system has many advantages: high production rate, steady operation, and being independent of the dilution rote. They also indicate that the diffusion of substrate is a constraint to the production of metablite.展开更多
采用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)技术、16 S rRNA和内在转录间隔区(ITS)高通量测序技术,探究了安化红茶的深发酵和浅发酵工艺过程中红茶的香气特征成分和微生物多样性。结果表明,在不同发酵工艺的安化红茶中共检测...采用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)技术、16 S rRNA和内在转录间隔区(ITS)高通量测序技术,探究了安化红茶的深发酵和浅发酵工艺过程中红茶的香气特征成分和微生物多样性。结果表明,在不同发酵工艺的安化红茶中共检测出82种挥发性成分,主要为醇类、酯类和醛类,占比约为85%;挥发性成分总量随着加工过程的进行呈现先增加后降低的趋势。以气味活度值(OAV)>1且变量投影重要性(VIP)>1为依据,在浅发酵与深发酵工艺的安化红茶中筛选出柠檬醛、芳樟醇、苯乙醇、苯乙醛、水杨酸甲酯、β-月桂烯等14种关键差异挥发性成分。安化红茶的优势细菌为鞘氨醇单胞菌属(Sphingomonas)、甲基杆菌属(Methylobacterium)、橙黄单胞菌属(Aureimonas),优势真菌为枝孢属(Cladosporium)、链格孢属(Alternaria)、Setophoma。橙黄单胞菌属、肠杆菌属(Enterobacter)、曲霉属(Aspergillus)、Pseudopithomyces与不同发酵过程显著相关。研究结果为安化红茶科学加工、风味解析提供理论参考与依据。展开更多
基金Supported by the Postdoctoral Innovation Fund of Shandong Province(201303032)the Independent Innovation Projects of Shandong Province(2012CX20505)the National 863 High Technology Project of the Ministry of Science and Technology of China(2012AA021201)
文摘Biosensors, which are the products of the biotechnology industry, are among the key projects of the 7th, 8th, and 9th Fiveyear Plans of China Science ＆ Technology Developing Programs, respectively, and they play an important role in developing and reforming traditional biotechnology. SBA series biosensor analyzer, as the only one commercial biosensor in China, has attracted lots of attention in the process of information gathering and measurement for biological industry with the development of technology and society. In this paper, we presented an overview of the most important contributions dealing with the monitoring of the biochemical analytes in fermentation processes using SBA series biosensor analyzers in China. Future trends of the biosensor analyzer in China were also mentioned in the last section.
文摘This paper theoretically studies the continuous immobilized cell (IMC) fer mentation system. A kinetic model of IMC is established, and the relational expressions between production rate, substrate concentration, biomass concentration and dilution rate in the IMC continuous stirred tank reactor (CSTR) are derived. These equations and some numerical calculations show that as compared with the free cell system the IMC system has many advantages: high production rate, steady operation, and being independent of the dilution rote. They also indicate that the diffusion of substrate is a constraint to the production of metablite.
文摘采用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)技术、16 S rRNA和内在转录间隔区(ITS)高通量测序技术,探究了安化红茶的深发酵和浅发酵工艺过程中红茶的香气特征成分和微生物多样性。结果表明,在不同发酵工艺的安化红茶中共检测出82种挥发性成分,主要为醇类、酯类和醛类,占比约为85%;挥发性成分总量随着加工过程的进行呈现先增加后降低的趋势。以气味活度值(OAV)>1且变量投影重要性(VIP)>1为依据,在浅发酵与深发酵工艺的安化红茶中筛选出柠檬醛、芳樟醇、苯乙醇、苯乙醛、水杨酸甲酯、β-月桂烯等14种关键差异挥发性成分。安化红茶的优势细菌为鞘氨醇单胞菌属(Sphingomonas)、甲基杆菌属(Methylobacterium)、橙黄单胞菌属(Aureimonas),优势真菌为枝孢属(Cladosporium)、链格孢属(Alternaria)、Setophoma。橙黄单胞菌属、肠杆菌属(Enterobacter)、曲霉属(Aspergillus)、Pseudopithomyces与不同发酵过程显著相关。研究结果为安化红茶科学加工、风味解析提供理论参考与依据。
