To investigate inhibitory effect of recombinant transforming growth factora-pseudomonas exotoxin fusion protein (TGFInhibitory effect of TGF_α-PE40; TP40) on neointimal proliferation following arterial injury.Methods...To investigate inhibitory effect of recombinant transforming growth factora-pseudomonas exotoxin fusion protein (TGFInhibitory effect of TGF_α-PE40; TP40) on neointimal proliferation following arterial injury.Methods: Forty-eight male rabbits fed with rich cholesterol diet were randomized into the treatment group(n= 24)and the control group (n=24). The rabbits in the treatment group were treated by local adiministration of TP40 (30 μg) 24 h after arterial injury, and the control group were treated by saline. LM and computer image analysis were used to study the rabbit arterial segments 2, 4 and & weeks after treatment. Results: Irregular thickening of the arterial intima. large amounts of smooth muscle cells within the neointima, and stenosis of the arterial cavity in the control group, and significant inhibition of intimal proliferation and no stenosis of the arterial cavity in the treatment group were observed microscopically. Computer image analysis showed that the neointimal area and the ratio of neointimal/medial area of the treatment group at 2, 4 and 8weeks after treatment were signifantly smaller than those of the control groups (P<0. 01 ). COnclusion: The results suggest that TP40 can significantly inhibit neointimal proliferation following carotid arterial injury.展开更多
为治疗产气荚膜梭菌感染引起的疾病,研究制备了产气荚膜梭菌多价高效抗毒素血清。试验采用C、D型产气荚膜梭菌标准菌株,制备了高浓度外毒素和灭活疫苗,作为免疫原多次免疫绵羊,通过间接ELISA法监测绵羊抗体水平变化,采用小鼠中和试验检...为治疗产气荚膜梭菌感染引起的疾病,研究制备了产气荚膜梭菌多价高效抗毒素血清。试验采用C、D型产气荚膜梭菌标准菌株,制备了高浓度外毒素和灭活疫苗,作为免疫原多次免疫绵羊,通过间接ELISA法监测绵羊抗体水平变化,采用小鼠中和试验检验绵羊抗毒素血清保护效果。结果表明:制备的高效价抗C、D型产气荚膜梭菌毒素血清每0.1 m L血清能中和400个C型毒素对小鼠的MLD和600个D型毒素MLD,有良好的应用前景。展开更多
文摘To investigate inhibitory effect of recombinant transforming growth factora-pseudomonas exotoxin fusion protein (TGFInhibitory effect of TGF_α-PE40; TP40) on neointimal proliferation following arterial injury.Methods: Forty-eight male rabbits fed with rich cholesterol diet were randomized into the treatment group(n= 24)and the control group (n=24). The rabbits in the treatment group were treated by local adiministration of TP40 (30 μg) 24 h after arterial injury, and the control group were treated by saline. LM and computer image analysis were used to study the rabbit arterial segments 2, 4 and & weeks after treatment. Results: Irregular thickening of the arterial intima. large amounts of smooth muscle cells within the neointima, and stenosis of the arterial cavity in the control group, and significant inhibition of intimal proliferation and no stenosis of the arterial cavity in the treatment group were observed microscopically. Computer image analysis showed that the neointimal area and the ratio of neointimal/medial area of the treatment group at 2, 4 and 8weeks after treatment were signifantly smaller than those of the control groups (P<0. 01 ). COnclusion: The results suggest that TP40 can significantly inhibit neointimal proliferation following carotid arterial injury.
文摘为治疗产气荚膜梭菌感染引起的疾病,研究制备了产气荚膜梭菌多价高效抗毒素血清。试验采用C、D型产气荚膜梭菌标准菌株,制备了高浓度外毒素和灭活疫苗,作为免疫原多次免疫绵羊,通过间接ELISA法监测绵羊抗体水平变化,采用小鼠中和试验检验绵羊抗毒素血清保护效果。结果表明:制备的高效价抗C、D型产气荚膜梭菌毒素血清每0.1 m L血清能中和400个C型毒素对小鼠的MLD和600个D型毒素MLD,有良好的应用前景。
