Currently,research on N^(6)-methyladenine(m^(6)A)is extensive in the field of oncology,while studies involving m^(6)A and skin diseases remain relatively limited.Based on existing reports,we searched PubMed and Web of...Currently,research on N^(6)-methyladenine(m^(6)A)is extensive in the field of oncology,while studies involving m^(6)A and skin diseases remain relatively limited.Based on existing reports,we searched PubMed and Web of Science for literature related to m^(6)A and dermatological conditions.Analysis of citation counts and journal impact factors revealed a significant upward trend in the volume of m^(6)A-related research.Term frequency analysis of titles and abstracts indicated that studies mainly focus on skin tumors and inflammatory or immune-related skin diseases,particularly melanoma,psoriasis,and skin development.Transcriptomic data from the Gene Expression Omnibus(GEO)were analyzed,revealing differential expression of m^(6)A-related genes in 4 types of skin tumors(including squamous cell carcinoma and basal cell carcinoma)as well as in inflammatory skin diseases such as psoriasis and atopic dermatitis,and potential mechanisms of action were also explored.Findings suggest that m^(6)A modifications exhibit heterogeneity between neoplastic and nonneoplastic skin diseases.However,the regulatory mechanisms of m^(6)A dynamic modifications on key genes involved in dermatological disorders remain unclear and warrant further investigation.展开更多
Background The bromodomain(BRD) proteins play a pivotal role in regulating gene expression by recognizing acetylated lysine residues and acting as chromatin-associated post-translational modification-inducing proteins...Background The bromodomain(BRD) proteins play a pivotal role in regulating gene expression by recognizing acetylated lysine residues and acting as chromatin-associated post-translational modification-inducing proteins. Although BRD proteins have been extensively studied in mammals, they have also been characterized in plants like Arabidopsis thaliana and Oryza sativa, where they regulate stress-responsive genes related to drought, salinity, and cold. However, their roles in cotton species remain unexplored.Results In this genome-wide comparative analysis, 145 BRD genes were identified in the tetraploid species(Gossypium hirsutum and G. barbadense), compared with 82 BRD genes in their diploid progenitors(G. arboreum and G. raimondii), indicating that polyploidization significantly influenced BRD gene evolution. Gene duplication analysis revealed 78.85% of duplications were segmental and 21.15% were tandem among 104 in-paralogous gene pairs, contributing to BRD gene expansion. Gene structure, motif, and domain analyses demonstrated that most genes were intron-less and conserved throughout evolution. Syntenic analysis revealed a greater number of orthologous gene pairs in the Dt sub-genome than in the At sub-genome. The abundance of regulatory, hormonal, and defense-related cis-regulatory elements in the promoter region suggests that BRD genes play a role in both biotic and abiotic stress responses. Protein-protein interaction analysis indicated that global transcription factor group E(GTE) transcription factors regulate BRD genes. Expression analysis revealed that BRD genes are predominantly involved in ovule development, with some genes displaying specific expression patterns under heat, cold, and salt stress. Furthermore, qRT-PCR analysis demonstrated significant differential expression of BRD genes between the tolerant and sensitive genotype, underscoring their potential role in mediating drought and salinity stress responses.Conclusions This study provides valuable insights into the evolution of BRD genes across species and their roles in abiotic stress tolerance, highlighting their potential in breeding programs to develop drought and salinity tolerant cotton varieties.展开更多
Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some ...Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer(LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1 A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1 A and TERT genes with relatively high sensitivity(80.0 %) and specificity(88.0 %) of LC detection in tumor DNA.展开更多
The mechanism of health effects caused by organohalogen pollutants, e.g., toxins from electronic waste(e-waste), is poorly understood. We supposed that micro RNAs(mi RNAs), an important post-transcriptional regulator,...The mechanism of health effects caused by organohalogen pollutants, e.g., toxins from electronic waste(e-waste), is poorly understood. We supposed that micro RNAs(mi RNAs), an important post-transcriptional regulator, could play a role in this process. In this study, fasting peripheral blood samples were collected from residents living at an e-waste site in northern China and a nearby reference population. Concentrations of e-waste related organohalogen pollutants in plasma from the exposure group were higher than the corresponding measurement in the reference group. Correspondingly, sixty mi RNAs in plasma showed > 2-fold change between the two groups in microarray analysis. Among them, mi R-125a-5p was confirmed to be upregulated by q RT-PCR and its validated targets were enriched in responses to xenobiotics and cancer related pathways. Furthermore, significant positive correlations were found between levels of mi R-125a-5p in plasma and reactive oxygen species(ROS) in polymorphonuclear neutrophil leukocytes(P < 0.05). These evidences suggested oxidative stress might be an intermediate between e-waste related POPs exposure and alteration of plasma mi RNA.展开更多
miRNA can regulate development and milk yield of the mammary gland through epigenetic mechanism, miRNA can directly and indirectly modulate the activity of the epigenetic machinery, target genes through post-inhibitio...miRNA can regulate development and milk yield of the mammary gland through epigenetic mechanism, miRNA can directly and indirectly modulate the activity of the epigenetic machinery, target genes through post-inhibition of translation initiation, mediate miRNA decay, target genes and inhibit the positive regulation, regulate tone modification, and regulate DNA methylation of target genes. Here we reviewed the role of miRNAs in mammary gland development and lactation. Researching miRNA in mammary gland development and lactation process, and understanding the response of the epigenetic mechanisms to external stimuli will be an important necessity to devise new technologies for maximizing their activity and milk production in the dairy cow.展开更多
Introduction Post-translational modifications of core histones have emerged as a critical player in dynamical regulation of gene expression and accurate chromatin structures<sup>[1-2]</sup>.In recent years...Introduction Post-translational modifications of core histones have emerged as a critical player in dynamical regulation of gene expression and accurate chromatin structures<sup>[1-2]</sup>.In recent years it has been demonstrated that,histone lysine methylation is particularly prominent as one of the most important epigenetic modifications during cell cycles,development and differentiation,and in response to external stimuli,e.g.exogenous growth factors and mechanical stimulation.This epigenetic modification may also be an early event that regulates the gene expression dur-展开更多
Biophysical factors can regulate many aspects of cell functions,including proliferation,migration and differentiation.In general,biophysical factors activate a myriad of signaling events;however,whether there is a com...Biophysical factors can regulate many aspects of cell functions,including proliferation,migration and differentiation.In general,biophysical factors activate a myriad of signaling events;however,whether there is a common paradigm for various mechnotransduction processes is not clear.Here we use cell reprogramming as a model to address this issue.Previous studies have shown that biochemical factors can help reprogram somatic cells into pluripotent stem cells,but the role of biophysical factors during this process remains unknown.We show,for the first time,that biophysical cues,in the form of micropatterned surfaces,can replace the effects of small molecule epigenetic modifiers and significantly improve the reprogramming efficiency.展开更多
OBJECTIVE To detect the underlying mechanism of time window for estrogen(E2)replacement treating cognitive decline.METHODS E2 begun 1 week after the ovariectomy(OVXST)or 3 months after the ovariectomy(OVXLT).Learning ...OBJECTIVE To detect the underlying mechanism of time window for estrogen(E2)replacement treating cognitive decline.METHODS E2 begun 1 week after the ovariectomy(OVXST)or 3 months after the ovariectomy(OVXLT).Learning and memory ability were examined by trace fear memory test and inhibitory avoidance test.LTP and LTD were detected by MED64.High throughput gene expression sequencing and microRNA(miR NA)sequencing were used to detecte the differently expressed genes between OVXSTand OVXLTafter estrogen treatment.RESULTS Subcutaneous injection of E2 improved fear memory formation in both 1 week after ovariectomy(OVXST)mice or 3 months after ovariectomy(OVXLT)mice.However,for fear memory extinction,facilitated by E2 in OVXSTmice,but impaired by E2 in OVXLTmice.Further researches showed in medial prefrontal cortex(mPFC),estrogen facilitates LTD in OVXSTmice but impairs LTD in OVXLTmice.Results of highthroughput sequencings of mR NA and miRNA in mPFC from sham,OVXSTmice,E2 treated OVXST mice,OVXLTmice,and E2 treated OVXLTmice indicated decreased miR-221-5 p expression in OVXLTmice compared with OVXSTmice.In OVXLT mice,miR-221-5 p could be further reduced by E2 treatment.Additionally,miR-221-5 p targeted neuralized E3 ubiquitin protein ligase 1 a/b(Neurl1 a/b)m RNA.Decreased miR-221-5 p will promotes cannabinoid receptor 1(CB1)ubiquitination through up-regulating Neurl1 a/b protein levels in E2 treated OVXLTmice,which disrupted the retrograde endocanabinoids system.Replenishing miR-221-5 p or treating with CB1 agonist rescued the fear extinction impairment in E2 treated OVXLTmice.CONCLUSION These results uncovered a epigenetic change after long term E2 responsible for failure of E2 improving cognitive performance in OVXLTmice,moreover miR-221-5 p and CB1 agonist as potential targets for prolonging the time window for E2 replacement therapy.展开更多
Precision therapy in the field of oncology is rapidly developing. Numerous somatic genetic markers in eg tyrosine kinase receptors or transcription factors have been identified to be indicative for the treatment with ...Precision therapy in the field of oncology is rapidly developing. Numerous somatic genetic markers in eg tyrosine kinase receptors or transcription factors have been identified to be indicative for the treatment with anti-cancer drugs. In contrast, only some recommendations have been developed considering hereditary variants in drug metabolizing enzymes such as TPMT, DYPD or UGT1A1. Although a huge knowledge has been gained on the association of drug transporters variants such as ABCB1 or ABCG2 and clinical outcome, the overall data is inconsistent and the predictability of the related phenotype is low. However, there is increasing evidence that individual phenotypic differences may result not only from genetics, but also from epigenetic alterations such as histone-acetylation or DNA-methylation. Moreover,interactions with non-coding RNAs contribute to protein expression and may modulate drug action. Currently intriguing developments of novel therapeutic approaches through epigenetic drugs are emerging. The overall complexity of epigenetics in drug action is so far only little understood. Of significant importance are the consequences of mi RNA interaction for drug resistance in cancer by regulating target genes and efflux transporters. Further intriguing findings address DNAmethylation as modifier of transporter function and its consequences in cancer development and treatment. The progress of science may lead to the discovery of rare, but functionally relevant SNPs and a better understanding of multiple genomic, epigenomic as well as phenotypic factors, contributing to drug response in malignancies.展开更多
Epigenetic Methods in Neuroscience Research(ISBN:978-1-4939-2753-1,e Book ISBN:978-1-4939-2754-8)was published by Springer in 2016.The editor of this book is Nina N.Karpova,Neuroscience Center,University of Helsinki.T...Epigenetic Methods in Neuroscience Research(ISBN:978-1-4939-2753-1,e Book ISBN:978-1-4939-2754-8)was published by Springer in 2016.The editor of this book is Nina N.Karpova,Neuroscience Center,University of Helsinki.This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system.Epigenetic guides readers through methods for the analyses of chromatin remodeling,transposable elements,展开更多
Epilepsy Towards the Next Decade(ISBN:978-3-319-12282-3,eBook ISBN:978-3-319-12283-0)was published by Springer International Publishing in 2015.The editor of this book is Pasquale Striano,Pediatric Neurology and Muscu...Epilepsy Towards the Next Decade(ISBN:978-3-319-12282-3,eBook ISBN:978-3-319-12283-0)was published by Springer International Publishing in 2015.The editor of this book is Pasquale Striano,Pediatric Neurology and Muscular Diseases Unit,University of Genoa.This volume is a comprehensive collection of the most recent knowledge on the biological bases of various kinds of展开更多
Neuropsychiatric disorders arise from complex interactions between genetic and environmental factors.DNA methylation,a reversible and environmentally responsive epigenetic regulatory mechanism,serves as a crucial brid...Neuropsychiatric disorders arise from complex interactions between genetic and environmental factors.DNA methylation,a reversible and environmentally responsive epigenetic regulatory mechanism,serves as a crucial bridge linking environmental exposure,gene expression regulation,and neurobehavioral outcomes.During long-duration deep-space missions,astronauts face multiple stressors-including microgravity,cosmic radiation,circadian rhythm disruption,and social isolation,which can induce alterations in DNA methylation and increase the risk of neuropsychiatric disorders.Genome-wide DNA methylation research can be divided into 3 major methodological stages:Study design,sample preparation and detection,and data analysis,each of which can be applied to astronaut neuropsychiatric health monitoring.Systematic comparison of the Illumina MethylationEPIC array and whole-genome bisulfite sequencing reveals their complementary strengths in terms of genomic coverage,resolution,cost,and application scenarios:the array method is cost-effective and suitable for large-scale population studies and longitudinal monitoring,whereas sequencing provides higher resolution and coverage and is more suitable for constructing detailed methylation maps and characterizing individual variation.Furthermore,emerging technologies such as single-cell methylation sequencing,nanopore long-read sequencing,and machine-learning-based multi-omics integration are expected to greatly enhance the precision and interpretability of epigenetic studies.These methodological advances provide key support for establishing DNAmethylation-based monitoring systems for neuropsychiatric risk in astronauts and lay an epigenetic foundation for safeguarding neuropsychiatric health during future long-term deep-space missions.展开更多
基金supported by the National Natural Science Foundation,China(82103704).
文摘Currently,research on N^(6)-methyladenine(m^(6)A)is extensive in the field of oncology,while studies involving m^(6)A and skin diseases remain relatively limited.Based on existing reports,we searched PubMed and Web of Science for literature related to m^(6)A and dermatological conditions.Analysis of citation counts and journal impact factors revealed a significant upward trend in the volume of m^(6)A-related research.Term frequency analysis of titles and abstracts indicated that studies mainly focus on skin tumors and inflammatory or immune-related skin diseases,particularly melanoma,psoriasis,and skin development.Transcriptomic data from the Gene Expression Omnibus(GEO)were analyzed,revealing differential expression of m^(6)A-related genes in 4 types of skin tumors(including squamous cell carcinoma and basal cell carcinoma)as well as in inflammatory skin diseases such as psoriasis and atopic dermatitis,and potential mechanisms of action were also explored.Findings suggest that m^(6)A modifications exhibit heterogeneity between neoplastic and nonneoplastic skin diseases.However,the regulatory mechanisms of m^(6)A dynamic modifications on key genes involved in dermatological disorders remain unclear and warrant further investigation.
文摘Background The bromodomain(BRD) proteins play a pivotal role in regulating gene expression by recognizing acetylated lysine residues and acting as chromatin-associated post-translational modification-inducing proteins. Although BRD proteins have been extensively studied in mammals, they have also been characterized in plants like Arabidopsis thaliana and Oryza sativa, where they regulate stress-responsive genes related to drought, salinity, and cold. However, their roles in cotton species remain unexplored.Results In this genome-wide comparative analysis, 145 BRD genes were identified in the tetraploid species(Gossypium hirsutum and G. barbadense), compared with 82 BRD genes in their diploid progenitors(G. arboreum and G. raimondii), indicating that polyploidization significantly influenced BRD gene evolution. Gene duplication analysis revealed 78.85% of duplications were segmental and 21.15% were tandem among 104 in-paralogous gene pairs, contributing to BRD gene expansion. Gene structure, motif, and domain analyses demonstrated that most genes were intron-less and conserved throughout evolution. Syntenic analysis revealed a greater number of orthologous gene pairs in the Dt sub-genome than in the At sub-genome. The abundance of regulatory, hormonal, and defense-related cis-regulatory elements in the promoter region suggests that BRD genes play a role in both biotic and abiotic stress responses. Protein-protein interaction analysis indicated that global transcription factor group E(GTE) transcription factors regulate BRD genes. Expression analysis revealed that BRD genes are predominantly involved in ovule development, with some genes displaying specific expression patterns under heat, cold, and salt stress. Furthermore, qRT-PCR analysis demonstrated significant differential expression of BRD genes between the tolerant and sensitive genotype, underscoring their potential role in mediating drought and salinity stress responses.Conclusions This study provides valuable insights into the evolution of BRD genes across species and their roles in abiotic stress tolerance, highlighting their potential in breeding programs to develop drought and salinity tolerant cotton varieties.
基金supported by the Skolkovo Foundation(Under agreement NO.G102/16 06.12.2016г.)
文摘Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer(LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1 A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1 A and TERT genes with relatively high sensitivity(80.0 %) and specificity(88.0 %) of LC detection in tumor DNA.
基金National Natural Science Foundation of China(21322705,41121004,21190051,and 21177091)
文摘The mechanism of health effects caused by organohalogen pollutants, e.g., toxins from electronic waste(e-waste), is poorly understood. We supposed that micro RNAs(mi RNAs), an important post-transcriptional regulator, could play a role in this process. In this study, fasting peripheral blood samples were collected from residents living at an e-waste site in northern China and a nearby reference population. Concentrations of e-waste related organohalogen pollutants in plasma from the exposure group were higher than the corresponding measurement in the reference group. Correspondingly, sixty mi RNAs in plasma showed > 2-fold change between the two groups in microarray analysis. Among them, mi R-125a-5p was confirmed to be upregulated by q RT-PCR and its validated targets were enriched in responses to xenobiotics and cancer related pathways. Furthermore, significant positive correlations were found between levels of mi R-125a-5p in plasma and reactive oxygen species(ROS) in polymorphonuclear neutrophil leukocytes(P < 0.05). These evidences suggested oxidative stress might be an intermediate between e-waste related POPs exposure and alteration of plasma mi RNA.
基金Support by the Natural Science Foundation of China(31072103)
文摘miRNA can regulate development and milk yield of the mammary gland through epigenetic mechanism, miRNA can directly and indirectly modulate the activity of the epigenetic machinery, target genes through post-inhibition of translation initiation, mediate miRNA decay, target genes and inhibit the positive regulation, regulate tone modification, and regulate DNA methylation of target genes. Here we reviewed the role of miRNAs in mammary gland development and lactation. Researching miRNA in mammary gland development and lactation process, and understanding the response of the epigenetic mechanisms to external stimuli will be an important necessity to devise new technologies for maximizing their activity and milk production in the dairy cow.
基金supported in part by NIH HL098472 and NSF CBET0846429supported by China Scholarship Council as well
文摘Introduction Post-translational modifications of core histones have emerged as a critical player in dynamical regulation of gene expression and accurate chromatin structures<sup>[1-2]</sup>.In recent years it has been demonstrated that,histone lysine methylation is particularly prominent as one of the most important epigenetic modifications during cell cycles,development and differentiation,and in response to external stimuli,e.g.exogenous growth factors and mechanical stimulation.This epigenetic modification may also be an early event that regulates the gene expression dur-
文摘Biophysical factors can regulate many aspects of cell functions,including proliferation,migration and differentiation.In general,biophysical factors activate a myriad of signaling events;however,whether there is a common paradigm for various mechnotransduction processes is not clear.Here we use cell reprogramming as a model to address this issue.Previous studies have shown that biochemical factors can help reprogram somatic cells into pluripotent stem cells,but the role of biophysical factors during this process remains unknown.We show,for the first time,that biophysical cues,in the form of micropatterned surfaces,can replace the effects of small molecule epigenetic modifiers and significantly improve the reprogramming efficiency.
文摘OBJECTIVE To detect the underlying mechanism of time window for estrogen(E2)replacement treating cognitive decline.METHODS E2 begun 1 week after the ovariectomy(OVXST)or 3 months after the ovariectomy(OVXLT).Learning and memory ability were examined by trace fear memory test and inhibitory avoidance test.LTP and LTD were detected by MED64.High throughput gene expression sequencing and microRNA(miR NA)sequencing were used to detecte the differently expressed genes between OVXSTand OVXLTafter estrogen treatment.RESULTS Subcutaneous injection of E2 improved fear memory formation in both 1 week after ovariectomy(OVXST)mice or 3 months after ovariectomy(OVXLT)mice.However,for fear memory extinction,facilitated by E2 in OVXSTmice,but impaired by E2 in OVXLTmice.Further researches showed in medial prefrontal cortex(mPFC),estrogen facilitates LTD in OVXSTmice but impairs LTD in OVXLTmice.Results of highthroughput sequencings of mR NA and miRNA in mPFC from sham,OVXSTmice,E2 treated OVXST mice,OVXLTmice,and E2 treated OVXLTmice indicated decreased miR-221-5 p expression in OVXLTmice compared with OVXSTmice.In OVXLT mice,miR-221-5 p could be further reduced by E2 treatment.Additionally,miR-221-5 p targeted neuralized E3 ubiquitin protein ligase 1 a/b(Neurl1 a/b)m RNA.Decreased miR-221-5 p will promotes cannabinoid receptor 1(CB1)ubiquitination through up-regulating Neurl1 a/b protein levels in E2 treated OVXLTmice,which disrupted the retrograde endocanabinoids system.Replenishing miR-221-5 p or treating with CB1 agonist rescued the fear extinction impairment in E2 treated OVXLTmice.CONCLUSION These results uncovered a epigenetic change after long term E2 responsible for failure of E2 improving cognitive performance in OVXLTmice,moreover miR-221-5 p and CB1 agonist as potential targets for prolonging the time window for E2 replacement therapy.
文摘Precision therapy in the field of oncology is rapidly developing. Numerous somatic genetic markers in eg tyrosine kinase receptors or transcription factors have been identified to be indicative for the treatment with anti-cancer drugs. In contrast, only some recommendations have been developed considering hereditary variants in drug metabolizing enzymes such as TPMT, DYPD or UGT1A1. Although a huge knowledge has been gained on the association of drug transporters variants such as ABCB1 or ABCG2 and clinical outcome, the overall data is inconsistent and the predictability of the related phenotype is low. However, there is increasing evidence that individual phenotypic differences may result not only from genetics, but also from epigenetic alterations such as histone-acetylation or DNA-methylation. Moreover,interactions with non-coding RNAs contribute to protein expression and may modulate drug action. Currently intriguing developments of novel therapeutic approaches through epigenetic drugs are emerging. The overall complexity of epigenetics in drug action is so far only little understood. Of significant importance are the consequences of mi RNA interaction for drug resistance in cancer by regulating target genes and efflux transporters. Further intriguing findings address DNAmethylation as modifier of transporter function and its consequences in cancer development and treatment. The progress of science may lead to the discovery of rare, but functionally relevant SNPs and a better understanding of multiple genomic, epigenomic as well as phenotypic factors, contributing to drug response in malignancies.
文摘Epigenetic Methods in Neuroscience Research(ISBN:978-1-4939-2753-1,e Book ISBN:978-1-4939-2754-8)was published by Springer in 2016.The editor of this book is Nina N.Karpova,Neuroscience Center,University of Helsinki.This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system.Epigenetic guides readers through methods for the analyses of chromatin remodeling,transposable elements,
文摘Epilepsy Towards the Next Decade(ISBN:978-3-319-12282-3,eBook ISBN:978-3-319-12283-0)was published by Springer International Publishing in 2015.The editor of this book is Pasquale Striano,Pediatric Neurology and Muscular Diseases Unit,University of Genoa.This volume is a comprehensive collection of the most recent knowledge on the biological bases of various kinds of
基金supported by the National Natural Science Foundation(82022024)the Graduate Independent Innovation Project of Central South University(2022ZZTS0866),China.
文摘Neuropsychiatric disorders arise from complex interactions between genetic and environmental factors.DNA methylation,a reversible and environmentally responsive epigenetic regulatory mechanism,serves as a crucial bridge linking environmental exposure,gene expression regulation,and neurobehavioral outcomes.During long-duration deep-space missions,astronauts face multiple stressors-including microgravity,cosmic radiation,circadian rhythm disruption,and social isolation,which can induce alterations in DNA methylation and increase the risk of neuropsychiatric disorders.Genome-wide DNA methylation research can be divided into 3 major methodological stages:Study design,sample preparation and detection,and data analysis,each of which can be applied to astronaut neuropsychiatric health monitoring.Systematic comparison of the Illumina MethylationEPIC array and whole-genome bisulfite sequencing reveals their complementary strengths in terms of genomic coverage,resolution,cost,and application scenarios:the array method is cost-effective and suitable for large-scale population studies and longitudinal monitoring,whereas sequencing provides higher resolution and coverage and is more suitable for constructing detailed methylation maps and characterizing individual variation.Furthermore,emerging technologies such as single-cell methylation sequencing,nanopore long-read sequencing,and machine-learning-based multi-omics integration are expected to greatly enhance the precision and interpretability of epigenetic studies.These methodological advances provide key support for establishing DNAmethylation-based monitoring systems for neuropsychiatric risk in astronauts and lay an epigenetic foundation for safeguarding neuropsychiatric health during future long-term deep-space missions.
