In the face of increasingly serious environmental pollution,the health of human lung tissues is also facing serious threats.Mogroside IIE(M2E)is the main metabolite of sweetening agents mogrosides from the anti-tussiv...In the face of increasingly serious environmental pollution,the health of human lung tissues is also facing serious threats.Mogroside IIE(M2E)is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori.The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury(ALI).A lipopolysaccharide(LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting,co-immunoprecipitation,and quantitative real time-PCR analysis.The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA(Pla2g2a)-epidermal growth factor receptor(EGFR).The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation.In addition,M2E protected ALI induced with LPS against inflammatory and damage which were significantly dependent upon the downregulation of AKT and m TOR via the inhibition of Pla2g2a-EGFR.Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury,which may represent a promising strategy to treat ALI.展开更多
1文献来源 Becker A, Crombag L, Heideman DA, et al. Retreatment with Erlotinib： Regain of TKI sensitivity following a drug holiday for patients with NSCLC who initially responded to EGFR-TKI treatment [J]. Eur J Cance...1文献来源 Becker A, Crombag L, Heideman DA, et al. Retreatment with Erlotinib： Regain of TKI sensitivity following a drug holiday for patients with NSCLC who initially responded to EGFR-TKI treatment [J]. Eur J Cancer, 2011,47（17） ：2603-2606.2展开更多
1文献来源Zhang YX,Sheng J,Kang SY,et al.Patients with exon 19 deletion were associated with longer progression-free survival compared to those with L858R mutation after first-line EGFR-TKIs for advanced non-small cell...1文献来源Zhang YX,Sheng J,Kang SY,et al.Patients with exon 19 deletion were associated with longer progression-free survival compared to those with L858R mutation after first-line EGFR-TKIs for advanced non-small cell lung cancer：A meta-analysis[J].PLo S One,2014,9（9）：e107161.2证据水平1a。展开更多
EGFR(表皮生长因子受体)信号通路在非小细胞肺癌(Non small cell lung cancer NSCLC)的发生和发展中起重要的作用,激活后可促进肿瘤细胞的增生、分化、转移、血管生成及凋亡抑制。大约80%的NSCLC存在EGFR的表达、过度表达和突变,因此EGF...EGFR(表皮生长因子受体)信号通路在非小细胞肺癌(Non small cell lung cancer NSCLC)的发生和发展中起重要的作用,激活后可促进肿瘤细胞的增生、分化、转移、血管生成及凋亡抑制。大约80%的NSCLC存在EGFR的表达、过度表达和突变,因此EGFR是治疗NSCLC的理想靶点。通过检测EGFR的表达和突变状态能预测EGFR酪氨酸激酶抑制剂(EGFR-TKI)治疗的疗效,成为指导晚期NSCLC临床靶向治疗的重要生物标志物。EGFR基因的体细胞突变(somatic mutation)研究为肺癌的个体化治疗提供有力的支持,但EGFR基因胚系突变(germline mutation)的研究却开展的较少。本文在于总结国际上关于EGFR基因18～21号外显子的胚系突变的研究。展开更多
基金the National Natural Science Foundation(81773982,82003937)Youth Academic leaders of the Qinglan Project in Jiangsu province for financial support。
文摘In the face of increasingly serious environmental pollution,the health of human lung tissues is also facing serious threats.Mogroside IIE(M2E)is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori.The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury(ALI).A lipopolysaccharide(LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting,co-immunoprecipitation,and quantitative real time-PCR analysis.The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA(Pla2g2a)-epidermal growth factor receptor(EGFR).The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation.In addition,M2E protected ALI induced with LPS against inflammatory and damage which were significantly dependent upon the downregulation of AKT and m TOR via the inhibition of Pla2g2a-EGFR.Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury,which may represent a promising strategy to treat ALI.
文摘1文献来源 Becker A, Crombag L, Heideman DA, et al. Retreatment with Erlotinib： Regain of TKI sensitivity following a drug holiday for patients with NSCLC who initially responded to EGFR-TKI treatment [J]. Eur J Cancer, 2011,47（17） ：2603-2606.2
文摘1文献来源Zhang YX,Sheng J,Kang SY,et al.Patients with exon 19 deletion were associated with longer progression-free survival compared to those with L858R mutation after first-line EGFR-TKIs for advanced non-small cell lung cancer：A meta-analysis[J].PLo S One,2014,9（9）：e107161.2证据水平1a。
文摘EGFR(表皮生长因子受体)信号通路在非小细胞肺癌(Non small cell lung cancer NSCLC)的发生和发展中起重要的作用,激活后可促进肿瘤细胞的增生、分化、转移、血管生成及凋亡抑制。大约80%的NSCLC存在EGFR的表达、过度表达和突变,因此EGFR是治疗NSCLC的理想靶点。通过检测EGFR的表达和突变状态能预测EGFR酪氨酸激酶抑制剂(EGFR-TKI)治疗的疗效,成为指导晚期NSCLC临床靶向治疗的重要生物标志物。EGFR基因的体细胞突变(somatic mutation)研究为肺癌的个体化治疗提供有力的支持,但EGFR基因胚系突变(germline mutation)的研究却开展的较少。本文在于总结国际上关于EGFR基因18～21号外显子的胚系突变的研究。
