The characteristics of absorption, fluorescence, room temperature phosphorescence(RTP) spectra of meso tetrakis(4 N trimethylaminobenzyl)porphin(TAPP) and Palladium porphyrin(Pd TAPP) probe in the presence and absence...The characteristics of absorption, fluorescence, room temperature phosphorescence(RTP) spectra of meso tetrakis(4 N trimethylaminobenzyl)porphin(TAPP) and Palladium porphyrin(Pd TAPP) probe in the presence and absence of ct DNA has been studied. Pd TAPP shows a very weak RTP emission in aqueous solution and strong RTP emission in the presence of DNA. Maximum RTP was observed at pH=7, with maximum excitation and emission wavelengths at 414 nm and 684 nm, respectively. RTP decay follows a first order exponential equation, RTP lifetime (τ) of probe is (0.72±0.03) ms. The detection limit of ct DNA may reach 8.8×10 -7 mol/L. The relative standard deviation(RSD) is less than ±6.6% in the linear range.展开更多
This paper describes a new fluorescence probe technique for the determination ofDNA, which is based on a self-quenching course of high concentration of o-hydroxybenzoicacid or o-aminobenzoic acid. Studies involving ca...This paper describes a new fluorescence probe technique for the determination ofDNA, which is based on a self-quenching course of high concentration of o-hydroxybenzoicacid or o-aminobenzoic acid. Studies involving calf thymus (CT) DNA, salmon (SM) DNAand herring sperm(HS) DNA revealed that the differential value of fluorescence intensity inthe presence and absence of nucleic acid was proportional to the concentration of nucleic overthe range of about 15ng/mL^6.0μg/mL.展开更多
文摘The characteristics of absorption, fluorescence, room temperature phosphorescence(RTP) spectra of meso tetrakis(4 N trimethylaminobenzyl)porphin(TAPP) and Palladium porphyrin(Pd TAPP) probe in the presence and absence of ct DNA has been studied. Pd TAPP shows a very weak RTP emission in aqueous solution and strong RTP emission in the presence of DNA. Maximum RTP was observed at pH=7, with maximum excitation and emission wavelengths at 414 nm and 684 nm, respectively. RTP decay follows a first order exponential equation, RTP lifetime (τ) of probe is (0.72±0.03) ms. The detection limit of ct DNA may reach 8.8×10 -7 mol/L. The relative standard deviation(RSD) is less than ±6.6% in the linear range.
文摘This paper describes a new fluorescence probe technique for the determination ofDNA, which is based on a self-quenching course of high concentration of o-hydroxybenzoicacid or o-aminobenzoic acid. Studies involving calf thymus (CT) DNA, salmon (SM) DNAand herring sperm(HS) DNA revealed that the differential value of fluorescence intensity inthe presence and absence of nucleic acid was proportional to the concentration of nucleic overthe range of about 15ng/mL^6.0μg/mL.
