OBJECTIVE Vanadium is a necessary trace element in the human body and has a certain role in the treatment and prevention of diabetes.Organic vanadium is more easily absorbed and less toxic than inorganic vanadium.Thus...OBJECTIVE Vanadium is a necessary trace element in the human body and has a certain role in the treatment and prevention of diabetes.Organic vanadium is more easily absorbed and less toxic than inorganic vanadium.Thus,in this study,vanadium complex was synthesized from genistein which had good hypoglycemic activity and inor⁃ganic vanadium element,and its hypoglycemic activity and acute oral toxicity were studied.METHODS The vanadium genistein complex was synthesized by chelating vanadium with genistein in methanol and its structure was determined by LC-MS,atomic absorption spectroscopy,UV-visible spectroscopy,elemental and thermodynamic analysis.The antidiabetic activity of the complex was assessed in db/db mice and C57 mice by daily oral gavage for 4 weeks.These db/db mice were divided into test groups〔high(30 mg·kg^-1),medium(20 mg·kg^-1)and low(10 mg·kg^-1)dose group〕,model group,genistein group,inorganic vanadium group,each group of 8;8 C57 mice was for normal control group.The acute toxicity test was carried out on KM mice with this complex by a maximum limit dose method.Randomly 20 healthy KM mice were divided into negative control group and test group,each group of 10,male and female half.RESULTS The molecular structure of this complex was inferred as a complex(VL2)formed by two ligands and one vanadium element.It was found that its hypoglycemic activity was better than that of genistein and inorganic vanadium.The hypoglycemic activity of the high dose group was better than that of the medium dose group and low dose group.The complex can signifi⁃cantly improve the body mass of db/db mice,fasting blood glucose,random blood glucose,liver/body,kidney/body,and the performance of oral glucose tolerance test and insulin tolerance test in db/db mice.The morphology of liver,kidney,pancreas and skeletal muscle also had obvious improvement and repair.Effect on serum index of db/db mice showed that,total cholesterol,triglyceride,low-density lipoprotein cholesterol had no significant improvement compared with the model group,but high-density lipoprotein cholesterol of complex group had significant improvement compared with the model group.In addition,this complex did not produce any hazardous symptoms or deaths in acute oral toxicity test.CONCLUSION complex has good hypoglycemic activity in vivo,and did not have the potential toxicity.This would provide an important reference for the development of functional hypoglycemic foods.展开更多
A series of batch experiments were conducted in 125 mL serum bottles to assess the toxicity of different concentrations of ammonia nitrogen to the specific methanogenic activity of anaerobic granular sludge from upflo...A series of batch experiments were conducted in 125 mL serum bottles to assess the toxicity of different concentrations of ammonia nitrogen to the specific methanogenic activity of anaerobic granular sludge from upflow anaerobic sludge bed（UASB） and expanded granular sludge bed（EGSB） reactors. The effects of pH value and temperature on toxicity of ammonia nitrogen to anaerobes were investigated. The results show that the specific methanogenic activity of anaerobic granular sludge suffers inhibition from ammonia nitrogen, the concentrations of ammonia nitrogen that produce 50 % inhibition of specific methanogenic activity for sludge from UASB and EGSB reactor are 2.35 and 2.75 g/L, respectively. Hydrogen utilizing methanogens suffers less inhibition from ammonia mtrogen than that of acetate utilizing methanogens. Hydrogen-producing acetogens that utilize propionate and butyrate as substrates suffer serious inhibition from ammonia nitrogen. The toxicity of ammonia nitrogen to anaerobic granular sludge enhances when pH value and temperature increase. Anaerobic granular sludge can bear higher concentrations of ammonia nitrogen after being acclimated by ammonia nitrogen for 7 d.展开更多
Aim Platinum based combination regimens are first-line treatment option in treatment of NSCLC but the clinical utility has been limited because of their toxicities. Many reports indicated that patients with tumors can...Aim Platinum based combination regimens are first-line treatment option in treatment of NSCLC but the clinical utility has been limited because of their toxicities. Many reports indicated that patients with tumors can ben- efit from adjuvant chemotherapy drugs. The aim of this study was to confirm adjuvant chemotherapy of active protein P23 with docetaxel plus cisplatin （DP） against non-small cell lung cancer （NSCLC） by evaluating synergistic anti- tumor activity and attenuated effect. Methods In vivo SPC-A-1 xenograft model was established to evaluate antitu- mor activity and toxicity of P23 along or combination with DP. Evaluation indexes include the relative tumor prolif- eration rate, tumor growth inhibition rate, body weight, food consumption, hematological and biochemical analysis. Results P23 treatment showed inhibited tumor growth and increased tumor inhibition of DP treatment. No signifi- cant toxicity was found in P23-treated mice, but significant toxicity was found in DP-treated mice. P23 combination with DP could reduce toxicity of DP treatment by improving body weight and food consumption, and increasing the number of WBC and PLT, decreasing the level of ALT, AST and BUN. Conclusion P23 combined with DP treat- ment has additive effect which contributes to enhance tumor growth inhibition of DP treatment and attenuated effect which contributes to reduce toxicity of DP treatment. These findings indicate potential benefit for use of P23 adju- vant chemotherapy for NSCLC treatment.展开更多
林木腐烂病是苹果树、梨树和杨树等林木枝干的重要真菌性病害。为了筛选出对苹果树腐烂病菌Valsa mali var.mali、梨树腐烂病菌V.mali var.pyri和杨树腐烂病菌V.sordida等3种不同寄主腐烂病菌都能有效防控的杀菌剂,本研究开展室内毒力...林木腐烂病是苹果树、梨树和杨树等林木枝干的重要真菌性病害。为了筛选出对苹果树腐烂病菌Valsa mali var.mali、梨树腐烂病菌V.mali var.pyri和杨树腐烂病菌V.sordida等3种不同寄主腐烂病菌都能有效防控的杀菌剂,本研究开展室内毒力试验比较了7种杀菌剂对3种腐烂病病原菌菌丝生长和分生孢子萌发的抑制效果,并进一步通过田间活性测定试验比较7种杀菌剂对梨树腐烂病病斑扩展和分生孢子发生的防治效果,同时测定了增效剂8.6%聚乙二醇(PEG)对7种杀菌剂的增效作用。毒力测定结果表明,苯醚甲环唑、戊唑醇、吡唑醚菌酯和丙唑·多菌灵对3种腐烂病病原菌菌丝生长和分生孢子萌发的抑制作用较强,其中EC_(50)平均值最低的是苯醚甲环唑,而戊唑醇的MIC平均值最低,在0.33 mg/L浓度下对3种腐烂病病原菌的菌丝生长和分生孢子萌发抑制率均达到100%。田间试验结果表明,45%苯醚甲环唑SC、43%戊唑醇SC和35%丙唑·多菌灵SE对梨树腐烂病病斑扩展和分生孢子萌发的防治效果突出,其中45%苯醚甲环唑SC 30.00 mg/L对病斑扩展防治效果达到82.23%,孢子萌发抑制效果达到85.96%,田间防治效果最好。10%丙硫唑SC+8.6%PEG处理组对病斑扩展防治效果提高了15.39百分点,达到73.46%,分生孢子萌发抑制率提高了23.75百分点,达到83.06%,增效作用显著。本研究为苹果树、梨树和杨树等3种寄主腐烂病的化学防控提供了科学依据。展开更多
文摘OBJECTIVE Vanadium is a necessary trace element in the human body and has a certain role in the treatment and prevention of diabetes.Organic vanadium is more easily absorbed and less toxic than inorganic vanadium.Thus,in this study,vanadium complex was synthesized from genistein which had good hypoglycemic activity and inor⁃ganic vanadium element,and its hypoglycemic activity and acute oral toxicity were studied.METHODS The vanadium genistein complex was synthesized by chelating vanadium with genistein in methanol and its structure was determined by LC-MS,atomic absorption spectroscopy,UV-visible spectroscopy,elemental and thermodynamic analysis.The antidiabetic activity of the complex was assessed in db/db mice and C57 mice by daily oral gavage for 4 weeks.These db/db mice were divided into test groups〔high(30 mg·kg^-1),medium(20 mg·kg^-1)and low(10 mg·kg^-1)dose group〕,model group,genistein group,inorganic vanadium group,each group of 8;8 C57 mice was for normal control group.The acute toxicity test was carried out on KM mice with this complex by a maximum limit dose method.Randomly 20 healthy KM mice were divided into negative control group and test group,each group of 10,male and female half.RESULTS The molecular structure of this complex was inferred as a complex(VL2)formed by two ligands and one vanadium element.It was found that its hypoglycemic activity was better than that of genistein and inorganic vanadium.The hypoglycemic activity of the high dose group was better than that of the medium dose group and low dose group.The complex can signifi⁃cantly improve the body mass of db/db mice,fasting blood glucose,random blood glucose,liver/body,kidney/body,and the performance of oral glucose tolerance test and insulin tolerance test in db/db mice.The morphology of liver,kidney,pancreas and skeletal muscle also had obvious improvement and repair.Effect on serum index of db/db mice showed that,total cholesterol,triglyceride,low-density lipoprotein cholesterol had no significant improvement compared with the model group,but high-density lipoprotein cholesterol of complex group had significant improvement compared with the model group.In addition,this complex did not produce any hazardous symptoms or deaths in acute oral toxicity test.CONCLUSION complex has good hypoglycemic activity in vivo,and did not have the potential toxicity.This would provide an important reference for the development of functional hypoglycemic foods.
文摘A series of batch experiments were conducted in 125 mL serum bottles to assess the toxicity of different concentrations of ammonia nitrogen to the specific methanogenic activity of anaerobic granular sludge from upflow anaerobic sludge bed（UASB） and expanded granular sludge bed（EGSB） reactors. The effects of pH value and temperature on toxicity of ammonia nitrogen to anaerobes were investigated. The results show that the specific methanogenic activity of anaerobic granular sludge suffers inhibition from ammonia nitrogen, the concentrations of ammonia nitrogen that produce 50 % inhibition of specific methanogenic activity for sludge from UASB and EGSB reactor are 2.35 and 2.75 g/L, respectively. Hydrogen utilizing methanogens suffers less inhibition from ammonia mtrogen than that of acetate utilizing methanogens. Hydrogen-producing acetogens that utilize propionate and butyrate as substrates suffer serious inhibition from ammonia nitrogen. The toxicity of ammonia nitrogen to anaerobic granular sludge enhances when pH value and temperature increase. Anaerobic granular sludge can bear higher concentrations of ammonia nitrogen after being acclimated by ammonia nitrogen for 7 d.
文摘Aim Platinum based combination regimens are first-line treatment option in treatment of NSCLC but the clinical utility has been limited because of their toxicities. Many reports indicated that patients with tumors can ben- efit from adjuvant chemotherapy drugs. The aim of this study was to confirm adjuvant chemotherapy of active protein P23 with docetaxel plus cisplatin （DP） against non-small cell lung cancer （NSCLC） by evaluating synergistic anti- tumor activity and attenuated effect. Methods In vivo SPC-A-1 xenograft model was established to evaluate antitu- mor activity and toxicity of P23 along or combination with DP. Evaluation indexes include the relative tumor prolif- eration rate, tumor growth inhibition rate, body weight, food consumption, hematological and biochemical analysis. Results P23 treatment showed inhibited tumor growth and increased tumor inhibition of DP treatment. No signifi- cant toxicity was found in P23-treated mice, but significant toxicity was found in DP-treated mice. P23 combination with DP could reduce toxicity of DP treatment by improving body weight and food consumption, and increasing the number of WBC and PLT, decreasing the level of ALT, AST and BUN. Conclusion P23 combined with DP treat- ment has additive effect which contributes to enhance tumor growth inhibition of DP treatment and attenuated effect which contributes to reduce toxicity of DP treatment. These findings indicate potential benefit for use of P23 adju- vant chemotherapy for NSCLC treatment.
