Cancer metastasis is the leading cause of death in cancer patients worldwide and one of the major challenges in treating cancer.Circulating tumor cells(CTCs)play a pivotal role in cancer metastasis.However,the content...Cancer metastasis is the leading cause of death in cancer patients worldwide and one of the major challenges in treating cancer.Circulating tumor cells(CTCs)play a pivotal role in cancer metastasis.However,the content of CTCs in peripheral blood is minimal,so the detection of CTCs in real samples is extremely challenging.Therefore,efficient enrichment and early detection of CTCs are essential to achieve timely diagnosis of diseases.In this work,we constructed an innovative and sensitive single-nanoparticle collision electrochemistry(SNCE)biosensor for the detection of MCF-7 cells(human breast cancer cells)by immunomagnetic separation technique and liposome signal amplification strategy.Liposomes embedded with platinum nanoparticles(Pt NPs)were used as signal probes,and homemade gold ultramicroelectrodes(Au UME)were used as the working electrodes.The effective collision between Pt NPs and UME would produce distinguishable step-type current.MCF-7 cells were accurately quantified according to the relationship between cell concentration and collision frequency(the number of step-type currents generated per unit time),realizing highly sensitive and specific detection of MCF-7 cells.The SNCE biosensor has a linear range of 10 cells·mL^(-1)to 10^(5) cells·mL^(-1)with a detection limit as low as 5 cells·mL^(-1).In addition,the successful detection of MCF-7 cells in complex samples showed that the SNCE biosensors have great potential for patient sample detection.展开更多
磁性氧化铁纳米粒子(magnetic iron oxide nanoparticles,MIONs)已被广泛应用于靶向药物转运、磁共振成像(MRI)、癌细胞分离和富集及肿瘤靶向治疗等生物医学领域。随着MIONs在生物医学领域应用的不断深入,对MIONs在更低的毒性、更稳定...磁性氧化铁纳米粒子(magnetic iron oxide nanoparticles,MIONs)已被广泛应用于靶向药物转运、磁共振成像(MRI)、癌细胞分离和富集及肿瘤靶向治疗等生物医学领域。随着MIONs在生物医学领域应用的不断深入,对MIONs在更低的毒性、更稳定的胶体分散性、更好的生物相容性及更高的磁响应性等方面的性能也提出了更具体的要求。因此,除了材料合成本身需不断改进外,选择合适的修饰材料对MIONs进行表面改性和修饰是至关重要的。本文对MIONs表面修饰的方法和表面修饰的材料及其作用进行了综述,并着重阐述了MIONs在循环肿瘤细胞(circulating tumor cells,CTCs)分离中应用的研究进展。展开更多
基金supported by the National Natural Science Foundation of China(Nos.22274037,22376055 and 21904032)the Natural Science Foundation of Hubei Province(2022CFB383)。
文摘Cancer metastasis is the leading cause of death in cancer patients worldwide and one of the major challenges in treating cancer.Circulating tumor cells(CTCs)play a pivotal role in cancer metastasis.However,the content of CTCs in peripheral blood is minimal,so the detection of CTCs in real samples is extremely challenging.Therefore,efficient enrichment and early detection of CTCs are essential to achieve timely diagnosis of diseases.In this work,we constructed an innovative and sensitive single-nanoparticle collision electrochemistry(SNCE)biosensor for the detection of MCF-7 cells(human breast cancer cells)by immunomagnetic separation technique and liposome signal amplification strategy.Liposomes embedded with platinum nanoparticles(Pt NPs)were used as signal probes,and homemade gold ultramicroelectrodes(Au UME)were used as the working electrodes.The effective collision between Pt NPs and UME would produce distinguishable step-type current.MCF-7 cells were accurately quantified according to the relationship between cell concentration and collision frequency(the number of step-type currents generated per unit time),realizing highly sensitive and specific detection of MCF-7 cells.The SNCE biosensor has a linear range of 10 cells·mL^(-1)to 10^(5) cells·mL^(-1)with a detection limit as low as 5 cells·mL^(-1).In addition,the successful detection of MCF-7 cells in complex samples showed that the SNCE biosensors have great potential for patient sample detection.
