Effects of five incubation temperatures (25℃ ,28℃ ,31℃ ,35℃ and 37℃ for 24hours) and four incubation time periods (0.4,16 and 24 hours at 35℃) on isolated microspore culture of Chinese cabbage were studied. The ...Effects of five incubation temperatures (25℃ ,28℃ ,31℃ ,35℃ and 37℃ for 24hours) and four incubation time periods (0.4,16 and 24 hours at 35℃) on isolated microspore culture of Chinese cabbage were studied. The results showed that cultured microspores of Chinese cabbage developed into embryos at all the incubation temperatues from 28℃ to 37℃ ,but the best response to high temperature occured at 35℃. Among the four kinds of time periods, the highest yield of embryos was obtained at the 24h treatment. Therefore, the isolated microspore culture of Chinese cabbege ran be efficiently carried out at 35℃ for 24 hours.展开更多
A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD mar...A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660, were 7.7 cM and 8.38 cM apart from the TuMV resistant gene, respectively. The two RAPD fragments were converted to SCAR markers. SCAR markers were confined in germplasm.展开更多
“8407”is a disease resistant germplasm of Chinese cabbage which is highly resis-tant to turnip mosaic virus (TuMV),selected by the Vegetable Institute,Hebei Academy of Agri-cultural and Forestry Sciences.In 1985 and...“8407”is a disease resistant germplasm of Chinese cabbage which is highly resis-tant to turnip mosaic virus (TuMV),selected by the Vegetable Institute,Hebei Academy of Agri-cultural and Forestry Sciences.In 1985 and 1989,it was approved and accepted as one of the na-tional disease resistant germplasm of Chinese cabbage by the National Expert Group.The identifi-cation in 1989 showed that“8407”was highly resistant or immune to 19 TuMV strains from 10provinces/cities across the country.Therefore,it was regarded as the best germplasm both verti-cally and horizontally resistant to TuMV of Chinese cabbage.This germplasm was selected fromChang Paodan (Inner Mongolia)of Qingmaye system after 6 generations of successive self cross.Using the disease resistant germplasm of“8407”,we developed new multi-resistant varieties of“8361”and“8612”in 1983 and 1986 respectively,which was highly resistant to TuMV and downymildew and soft rot.These varieties have been extended to an areas of over 20000 ha.展开更多
Nutritional accumulation mainly occurres from growth stage 2 to stage 3(lotus-setting stage to early heading stage, 48 - 72 days after transplanting)in Chinese cabbage. The uptake ratio of N and K is nearly l:l,and th...Nutritional accumulation mainly occurres from growth stage 2 to stage 3(lotus-setting stage to early heading stage, 48 - 72 days after transplanting)in Chinese cabbage. The uptake ratio of N and K is nearly l:l,and their uptake amounts are obviously higher than that of P. Balanced fertilization .especially the suitable combination of N and K,is important for the production of high yield and high quality.展开更多
This paper describes using NIRS to determine CP,NDF,RS,VC in Chinese cabbage (Brassica campestris L. ssp pekinensis). Two hundred and forty-two samples of Chinese cabbage were collected from the main producing areas i...This paper describes using NIRS to determine CP,NDF,RS,VC in Chinese cabbage (Brassica campestris L. ssp pekinensis). Two hundred and forty-two samples of Chinese cabbage were collected from the main producing areas in China for calibration and prediction of the four constituents above. The results of calibration showed that the multiple correlation coefficients between NIRS and chemical methods were 0. 9958,0. 9852,0. 9821 and 0. 9814,and the standard errors were 0. 398,0. 134,0. 098 and 0. 51 ,respectively. The correlation coefficients of prediction were 0. 994,0. 978,0. 974 and 0. 981 ,and standard errors were 0. 353,0. 127, 0. 108 and 0. 548,respectively. These results indicate that NIRS can be used to determine the four constituents of Chinese cabbage as accurately as chemical methods.展开更多
The transcriptome-wide gene expression was compared between susceptible and resistant Chinese cabbage cultivars to identify genes that contributed to clubroot resistance. A higher number of differentially expressed ge...The transcriptome-wide gene expression was compared between susceptible and resistant Chinese cabbage cultivars to identify genes that contributed to clubroot resistance. A higher number of differentially expressed genes were detected in susceptible cultivars than in resistant cultivars. Fifty-six genes involved in cell wall modification, hormone signaling, root marphogenesis, nematodes response and cell proliferation were uniquely expressed in the resistant cultivars. Among them, 27 genes were involved in cell wall modification and hormone signaling, indicating that genes in these two types might play a vital role in the defense response to pathogen infection.展开更多
The ultraviolet-visible light spectrophotometer method was adopted to determine thioglucoside in cabbage with the seeds.Individual plant of cabbage was used as test materials,palladium chloride as complexing agent and...The ultraviolet-visible light spectrophotometer method was adopted to determine thioglucoside in cabbage with the seeds.Individual plant of cabbage was used as test materials,palladium chloride as complexing agent and sodium cellulose glycolate as dispersing agent.The results showed that palladiumd thioglucoside method could be taken as a quick,easy and precise quantitative analysis method to determine thioglucoside in cabbage.展开更多
Isolation of high-quality mitochondrial DNA（mtDNA） is an important premise for researching molecular mechanisms in cytoplasmic male sterility of cabbage（Brassica oleracea L.var.capitata）. An efficient protocol for...Isolation of high-quality mitochondrial DNA（mtDNA） is an important premise for researching molecular mechanisms in cytoplasmic male sterility of cabbage（Brassica oleracea L.var.capitata）. An efficient protocol for separation and purification of mitochondria and extraction of mitochondrial DNA（mtDNA） from etiolated tissues of cabbage was developed. We took a method combined mannitol density gradient with differential centrifugation, selected appropriate rotational speed, extended DNase I treating time and changed mitochondria cracking condition. The results showed that the extracted mitochondria in this protocol had complete structure, appeared to ellipsoid and had not been contaminated with other impurities under the Jannus Green B staining. The isolated mitochondrial DNA had high purity and yield through detecting the optical density, nuclear specific primer PCR and agarose gel electrophoresis. The results indicated that mitochondrial DNA extracted by this protocol had high quality and enabled to be used in futher genetic studies.展开更多
The endogenous polyamine levels were tested at every developmental stage in different ecotypes of the Chinese cabbage. The results showed that polyamincs (PAs) were decreased in the process of flower bud initiation an...The endogenous polyamine levels were tested at every developmental stage in different ecotypes of the Chinese cabbage. The results showed that polyamincs (PAs) were decreased in the process of flower bud initiation and fornfation, but the floral stem formation and elongation were accompied by PAs increase. The level of Spm was related to the bolting date of Chinese cabbage. During the inducing of flower bud initiation and elongation of floral stem, and the changes of Spm were reversed between early bolting date and late bolting cultivars, but they have the same requirment for Spd for the starting of flower bud initiation and the starting of bolting. The level of Spd changed little during entire developmental stage for early bolting cultivars, but a lot for late bolting cultivars. The time that the highest level of Put appeared is related to the bolting date of Chinese cabbage.展开更多
To explore the effects of the two chelating agents on the availability of calcium and phosphorus in black soil of vegetable fields and the growth of cabbage(Brassica pekinensis Rupr.)seedlings,humic acid(F)and ethylen...To explore the effects of the two chelating agents on the availability of calcium and phosphorus in black soil of vegetable fields and the growth of cabbage(Brassica pekinensis Rupr.)seedlings,humic acid(F)and ethylene diamine tetraacetic acid disodium salt(EDTA)were used as experiment materials,and two factors and three levels completely randomized experiment of the nine treatments was set.The experiments were carried out by soil incubation experiment and pot experiment to investigate the effects of the two chelating agents with different ratios and their interaction on soil and cabbage.The results showed that there were very significant differences among soil pH,soil available phosphorus content,cabbage biomass,calcium and phosphorus accumulation of cabbage under the interaction of the two chelating agents.It could improve pH of the soil and reduce the electric conductivity(EC)value of soil,when applied chelating agents of different proportions.The EC value of soil fluctuated during the incubation period.There were significant differences between pH and EC in the early incubation period,but there was no significant difference in the later stage.And there were significant differences between soil available calcium and available phosphorus content;there were significant differences between cabbage biomass and the accumulation of calcium and phosphorus in cabbage,and the data of F2E2 treatment were significantly higher than those of other treatments.In summary,under the conditions of the experiment,the available phosphorus content of black soil in vegetable fields,the biomass and accumulation of calcium and phosphorus in cabbage increased significantly in F2E2 treatment(appied 675 kg•hm-2 of lime,300 kg•hm-2 of humic acid and 150 kg•hm-2 of EDTA).展开更多
文摘Effects of five incubation temperatures (25℃ ,28℃ ,31℃ ,35℃ and 37℃ for 24hours) and four incubation time periods (0.4,16 and 24 hours at 35℃) on isolated microspore culture of Chinese cabbage were studied. The results showed that cultured microspores of Chinese cabbage developed into embryos at all the incubation temperatues from 28℃ to 37℃ ,but the best response to high temperature occured at 35℃. Among the four kinds of time periods, the highest yield of embryos was obtained at the 24h treatment. Therefore, the isolated microspore culture of Chinese cabbege ran be efficiently carried out at 35℃ for 24 hours.
文摘A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant). Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660, were 7.7 cM and 8.38 cM apart from the TuMV resistant gene, respectively. The two RAPD fragments were converted to SCAR markers. SCAR markers were confined in germplasm.
文摘“8407”is a disease resistant germplasm of Chinese cabbage which is highly resis-tant to turnip mosaic virus (TuMV),selected by the Vegetable Institute,Hebei Academy of Agri-cultural and Forestry Sciences.In 1985 and 1989,it was approved and accepted as one of the na-tional disease resistant germplasm of Chinese cabbage by the National Expert Group.The identifi-cation in 1989 showed that“8407”was highly resistant or immune to 19 TuMV strains from 10provinces/cities across the country.Therefore,it was regarded as the best germplasm both verti-cally and horizontally resistant to TuMV of Chinese cabbage.This germplasm was selected fromChang Paodan (Inner Mongolia)of Qingmaye system after 6 generations of successive self cross.Using the disease resistant germplasm of“8407”,we developed new multi-resistant varieties of“8361”and“8612”in 1983 and 1986 respectively,which was highly resistant to TuMV and downymildew and soft rot.These varieties have been extended to an areas of over 20000 ha.
文摘Nutritional accumulation mainly occurres from growth stage 2 to stage 3(lotus-setting stage to early heading stage, 48 - 72 days after transplanting)in Chinese cabbage. The uptake ratio of N and K is nearly l:l,and their uptake amounts are obviously higher than that of P. Balanced fertilization .especially the suitable combination of N and K,is important for the production of high yield and high quality.
文摘This paper describes using NIRS to determine CP,NDF,RS,VC in Chinese cabbage (Brassica campestris L. ssp pekinensis). Two hundred and forty-two samples of Chinese cabbage were collected from the main producing areas in China for calibration and prediction of the four constituents above. The results of calibration showed that the multiple correlation coefficients between NIRS and chemical methods were 0. 9958,0. 9852,0. 9821 and 0. 9814,and the standard errors were 0. 398,0. 134,0. 098 and 0. 51 ,respectively. The correlation coefficients of prediction were 0. 994,0. 978,0. 974 and 0. 981 ,and standard errors were 0. 353,0. 127, 0. 108 and 0. 548,respectively. These results indicate that NIRS can be used to determine the four constituents of Chinese cabbage as accurately as chemical methods.
基金Supported by the National Key Research and Development Project(2017YFD0101803)
文摘The transcriptome-wide gene expression was compared between susceptible and resistant Chinese cabbage cultivars to identify genes that contributed to clubroot resistance. A higher number of differentially expressed genes were detected in susceptible cultivars than in resistant cultivars. Fifty-six genes involved in cell wall modification, hormone signaling, root marphogenesis, nematodes response and cell proliferation were uniquely expressed in the resistant cultivars. Among them, 27 genes were involved in cell wall modification and hormone signaling, indicating that genes in these two types might play a vital role in the defense response to pathogen infection.
文摘The ultraviolet-visible light spectrophotometer method was adopted to determine thioglucoside in cabbage with the seeds.Individual plant of cabbage was used as test materials,palladium chloride as complexing agent and sodium cellulose glycolate as dispersing agent.The results showed that palladiumd thioglucoside method could be taken as a quick,easy and precise quantitative analysis method to determine thioglucoside in cabbage.
基金Supported by Funding of Utilization of Heterosis and Breeding of New Variety in Brassicaceous Vegetable(2012BAD02B01)
文摘Isolation of high-quality mitochondrial DNA（mtDNA） is an important premise for researching molecular mechanisms in cytoplasmic male sterility of cabbage（Brassica oleracea L.var.capitata）. An efficient protocol for separation and purification of mitochondria and extraction of mitochondrial DNA（mtDNA） from etiolated tissues of cabbage was developed. We took a method combined mannitol density gradient with differential centrifugation, selected appropriate rotational speed, extended DNase I treating time and changed mitochondria cracking condition. The results showed that the extracted mitochondria in this protocol had complete structure, appeared to ellipsoid and had not been contaminated with other impurities under the Jannus Green B staining. The isolated mitochondrial DNA had high purity and yield through detecting the optical density, nuclear specific primer PCR and agarose gel electrophoresis. The results indicated that mitochondrial DNA extracted by this protocol had high quality and enabled to be used in futher genetic studies.
基金The results is supported by Chinese natural science foundation
文摘The endogenous polyamine levels were tested at every developmental stage in different ecotypes of the Chinese cabbage. The results showed that polyamincs (PAs) were decreased in the process of flower bud initiation and fornfation, but the floral stem formation and elongation were accompied by PAs increase. The level of Spm was related to the bolting date of Chinese cabbage. During the inducing of flower bud initiation and elongation of floral stem, and the changes of Spm were reversed between early bolting date and late bolting cultivars, but they have the same requirment for Spd for the starting of flower bud initiation and the starting of bolting. The level of Spd changed little during entire developmental stage for early bolting cultivars, but a lot for late bolting cultivars. The time that the highest level of Put appeared is related to the bolting date of Chinese cabbage.
基金Supported by the Special Fund for Agro-scientific Research in Public Interest in China(201503119-06-01)。
文摘To explore the effects of the two chelating agents on the availability of calcium and phosphorus in black soil of vegetable fields and the growth of cabbage(Brassica pekinensis Rupr.)seedlings,humic acid(F)and ethylene diamine tetraacetic acid disodium salt(EDTA)were used as experiment materials,and two factors and three levels completely randomized experiment of the nine treatments was set.The experiments were carried out by soil incubation experiment and pot experiment to investigate the effects of the two chelating agents with different ratios and their interaction on soil and cabbage.The results showed that there were very significant differences among soil pH,soil available phosphorus content,cabbage biomass,calcium and phosphorus accumulation of cabbage under the interaction of the two chelating agents.It could improve pH of the soil and reduce the electric conductivity(EC)value of soil,when applied chelating agents of different proportions.The EC value of soil fluctuated during the incubation period.There were significant differences between pH and EC in the early incubation period,but there was no significant difference in the later stage.And there were significant differences between soil available calcium and available phosphorus content;there were significant differences between cabbage biomass and the accumulation of calcium and phosphorus in cabbage,and the data of F2E2 treatment were significantly higher than those of other treatments.In summary,under the conditions of the experiment,the available phosphorus content of black soil in vegetable fields,the biomass and accumulation of calcium and phosphorus in cabbage increased significantly in F2E2 treatment(appied 675 kg•hm-2 of lime,300 kg•hm-2 of humic acid and 150 kg•hm-2 of EDTA).
