In view of the influence by Fe3+,the reaction between paclitaxel and bovine serum albumin(BSA) in aqueous solution was studied by fluorescence spectroscopy and ultraviolet spectra.The binding constant K and the bindin...In view of the influence by Fe3+,the reaction between paclitaxel and bovine serum albumin(BSA) in aqueous solution was studied by fluorescence spectroscopy and ultraviolet spectra.The binding constant K and the binding sites n were measured according to suggested model. The binding distance r and transfer efficiency E between paclitaxel-iron(Ⅲ) and BSA were also obtained according to Fōrster theory of non-radiative energy transfer.The results indicated that there is a stronger binding force between paclitaxel-iron(Ⅲ) and BSA.展开更多
纳米银与牛血清白蛋白(BSA)均匀混合形成纳米银-BSA体系,运用荧光光谱,紫外吸收光谱,同步荧光光谱研究了注射用头孢美唑钠(Cefmetazole Sodium for Injection,CS)与BSA及纳米银-BSA体系的相互作用。头孢美唑钠对BSA具有荧光猝灭作用,其...纳米银与牛血清白蛋白(BSA)均匀混合形成纳米银-BSA体系,运用荧光光谱,紫外吸收光谱,同步荧光光谱研究了注射用头孢美唑钠(Cefmetazole Sodium for Injection,CS)与BSA及纳米银-BSA体系的相互作用。头孢美唑钠对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭,求出了猝灭常数,结合常数及结合位点数。在295K和302K时用热力学方程处理实验数据,求得了热力学参数△H、△G、和△S,头孢美唑钠与BSA之间的作用力主要为氢键和范德华力。头孢美唑钠对BSA中色氨酸和酪氨酸残基均有影响。纳米银的存在不改变头孢美唑钠对BSA的猝灭方式,但猝灭常数、结合常数、热力学参数及作用力均发生变化,在短时间内,纳米银的加入对BSA构象未见明显影响。展开更多
文摘In view of the influence by Fe3+,the reaction between paclitaxel and bovine serum albumin(BSA) in aqueous solution was studied by fluorescence spectroscopy and ultraviolet spectra.The binding constant K and the binding sites n were measured according to suggested model. The binding distance r and transfer efficiency E between paclitaxel-iron(Ⅲ) and BSA were also obtained according to Fōrster theory of non-radiative energy transfer.The results indicated that there is a stronger binding force between paclitaxel-iron(Ⅲ) and BSA.
文摘纳米银与牛血清白蛋白(BSA)均匀混合形成纳米银-BSA体系,运用荧光光谱,紫外吸收光谱,同步荧光光谱研究了注射用头孢美唑钠(Cefmetazole Sodium for Injection,CS)与BSA及纳米银-BSA体系的相互作用。头孢美唑钠对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭,求出了猝灭常数,结合常数及结合位点数。在295K和302K时用热力学方程处理实验数据,求得了热力学参数△H、△G、和△S,头孢美唑钠与BSA之间的作用力主要为氢键和范德华力。头孢美唑钠对BSA中色氨酸和酪氨酸残基均有影响。纳米银的存在不改变头孢美唑钠对BSA的猝灭方式,但猝灭常数、结合常数、热力学参数及作用力均发生变化,在短时间内,纳米银的加入对BSA构象未见明显影响。
