Dongnong50 was overexpressing TaDREB3, a stress-tolerance gene, and transgenic progenies with TaDREB3 showing stable heredity characters were obtained in this study. The seeds of T4 generation were sowed on saline lan...Dongnong50 was overexpressing TaDREB3, a stress-tolerance gene, and transgenic progenies with TaDREB3 showing stable heredity characters were obtained in this study. The seeds of T4 generation were sowed on saline land of which salt concentration was 0.19% and pH was 8.43, and agronomic traits including growth period, plant height, numbers of main nodes, branching numbers, pods per plant, numbers of seeds per plant, and weight of 100-seed were collected and comparing to control plants Dongnong50 were made. The impacts of TaDREB3 gene on agronomic traits of soybeans under high concentration of salt were discussed. The results showed that the growth period was not altered in transgenic line with TaDREB3 under high concentration of saline and alkali, the extremely significant or significant increase in the numbers of main nodes, pods per plant and in branching numbers and other agronomic traits in transgenic lines in comparing with non-transgenic lines was an indicator of their resistance to saline-alkali stress展开更多
The wild cotton cultivars and species have abundant genetic polymorphisms,and they possess lots of excellent genes,such as drought re sistance,insect resistance,fine and strong fiber,and so on.So cotton breeding exper...The wild cotton cultivars and species have abundant genetic polymorphisms,and they possess lots of excellent genes,such as drought re sistance,insect resistance,fine and strong fiber,and so on.So cotton breeding experts have widely carried out inter specific hybridization and obtained more than 20 type s of a dvanced generation hybrids with good quality and sta ble traits between wild resources and upland cotton.We called these upland cotton lines as introgression line s of interspecific hybridization.But they have not been completely exploited,especially lacked of theoretical research in the past.展开更多
Background Gossypium hirsutum undergoes rapid clonal propagation to regenerate a mature plant through tissue culture.However,the correlation between cotton leaf regeneration,callus induction,and regeneration ability w...Background Gossypium hirsutum undergoes rapid clonal propagation to regenerate a mature plant through tissue culture.However,the correlation between cotton leaf regeneration,callus induction,and regeneration ability was still obscure.In this research,cotton leaf regeneration level for 21 accessions in the field(new leaves)was observed after the first harvest,and a comparison between field regeneration level and callus induction with its regeneration capacity(new shoots and roots)for the same 21 accessions was carried out.Agronomic traits,including plant height,leaf area,fresh leaf weight,dry leaf weight,the number of flowers and bolls,and biochemical(proline content)and physiological(chlorophyll and carotenoid content)traits during the flowering stage of 21 upland cotton accessions,were investigated.Result A significant correlation between physiological parameters and callus induction was discovered.Callus induction and regeneration capacity of roots and shoots for hypocotyl,cotyledons,and shoot tip tissues were used to validate field leaf regeneration level after the first harvest.CCRI 24 showed significant leaf regeneration in the field and callus induction capacity through callus induction and regeneration.Conclusion We found a substantial relationship between field regeneration capability and callus induction with its regeneration capacity for the hypocotyl,cotyledons,and shoot tip.The results showed that ZS061,Lumian 378,Jimian 863,and ZS065 have the highest moisture retention capacity,while CCRI 24,Liaoyang Duomaomian,and Beizhe Gongshemian have the lowest moisture retention capacity.CCRI 24 has the highest leaf regeneration capacity in the field,while Beizhe Gongshemian has the lowest leaf regeneration capacity.All our result provides a clue for checking the regeneration capacity through leaf regeneration level in the field.展开更多
Seeding rate is an important management practice for soybean production.Chinese and U.S.soybean growers use different seeding rates,and breeders in the two countries have developed cultivars adapted to respective plan...Seeding rate is an important management practice for soybean production.Chinese and U.S.soybean growers use different seeding rates,and breeders in the two countries have developed cultivars adapted to respective plant densities.The objective of this study was to compare the effect of plant density on cultivars recently released in different breeding programs,using four cultivars developed in Liaoning,China and four in Ohio,USA.We used 3 plant density treatments(7.5,15.0,22.5 x 104 plants/hm2) and assessed yield and agronomic traits from 2004 to 2006 in Liaoning.There was no significant effect of plant density on yield for either group of the cultivars.The average yield of Ohio cultivars was higher than that of Liaoning cultivars,and there was no significant interaction between plant density and cultivar for all the assessed traits.The plant height of Liaoning cultivars was significantly higher than that of Ohio cultivars,and there was a significant effect of plant density on plant height.The average branch number of Ohio cultivars was larger than that of Liaoning cultivars;higher plant density reduced the branch number per plant greatly.Plant density had a signifi-cant effect on the node number and internode length,Liaoning cultivars generally had longer internode length.Plant density had a significant effect on seed yield:stem ratio,as the plant density increased the seed yield:stem ratio decreased for both groups of cultivars.However,100-seed weight was not affected by plant density.展开更多
基金Supported by Project of Education Department of Heilongjiang Province (11551048)
文摘Dongnong50 was overexpressing TaDREB3, a stress-tolerance gene, and transgenic progenies with TaDREB3 showing stable heredity characters were obtained in this study. The seeds of T4 generation were sowed on saline land of which salt concentration was 0.19% and pH was 8.43, and agronomic traits including growth period, plant height, numbers of main nodes, branching numbers, pods per plant, numbers of seeds per plant, and weight of 100-seed were collected and comparing to control plants Dongnong50 were made. The impacts of TaDREB3 gene on agronomic traits of soybeans under high concentration of salt were discussed. The results showed that the growth period was not altered in transgenic line with TaDREB3 under high concentration of saline and alkali, the extremely significant or significant increase in the numbers of main nodes, pods per plant and in branching numbers and other agronomic traits in transgenic lines in comparing with non-transgenic lines was an indicator of their resistance to saline-alkali stress
文摘The wild cotton cultivars and species have abundant genetic polymorphisms,and they possess lots of excellent genes,such as drought re sistance,insect resistance,fine and strong fiber,and so on.So cotton breeding experts have widely carried out inter specific hybridization and obtained more than 20 type s of a dvanced generation hybrids with good quality and sta ble traits between wild resources and upland cotton.We called these upland cotton lines as introgression line s of interspecific hybridization.But they have not been completely exploited,especially lacked of theoretical research in the past.
基金supported by Ministry of Agriculture and Rural Affairs(19221957).
文摘Background Gossypium hirsutum undergoes rapid clonal propagation to regenerate a mature plant through tissue culture.However,the correlation between cotton leaf regeneration,callus induction,and regeneration ability was still obscure.In this research,cotton leaf regeneration level for 21 accessions in the field(new leaves)was observed after the first harvest,and a comparison between field regeneration level and callus induction with its regeneration capacity(new shoots and roots)for the same 21 accessions was carried out.Agronomic traits,including plant height,leaf area,fresh leaf weight,dry leaf weight,the number of flowers and bolls,and biochemical(proline content)and physiological(chlorophyll and carotenoid content)traits during the flowering stage of 21 upland cotton accessions,were investigated.Result A significant correlation between physiological parameters and callus induction was discovered.Callus induction and regeneration capacity of roots and shoots for hypocotyl,cotyledons,and shoot tip tissues were used to validate field leaf regeneration level after the first harvest.CCRI 24 showed significant leaf regeneration in the field and callus induction capacity through callus induction and regeneration.Conclusion We found a substantial relationship between field regeneration capability and callus induction with its regeneration capacity for the hypocotyl,cotyledons,and shoot tip.The results showed that ZS061,Lumian 378,Jimian 863,and ZS065 have the highest moisture retention capacity,while CCRI 24,Liaoyang Duomaomian,and Beizhe Gongshemian have the lowest moisture retention capacity.CCRI 24 has the highest leaf regeneration capacity in the field,while Beizhe Gongshemian has the lowest leaf regeneration capacity.All our result provides a clue for checking the regeneration capacity through leaf regeneration level in the field.
文摘Seeding rate is an important management practice for soybean production.Chinese and U.S.soybean growers use different seeding rates,and breeders in the two countries have developed cultivars adapted to respective plant densities.The objective of this study was to compare the effect of plant density on cultivars recently released in different breeding programs,using four cultivars developed in Liaoning,China and four in Ohio,USA.We used 3 plant density treatments(7.5,15.0,22.5 x 104 plants/hm2) and assessed yield and agronomic traits from 2004 to 2006 in Liaoning.There was no significant effect of plant density on yield for either group of the cultivars.The average yield of Ohio cultivars was higher than that of Liaoning cultivars,and there was no significant interaction between plant density and cultivar for all the assessed traits.The plant height of Liaoning cultivars was significantly higher than that of Ohio cultivars,and there was a significant effect of plant density on plant height.The average branch number of Ohio cultivars was larger than that of Liaoning cultivars;higher plant density reduced the branch number per plant greatly.Plant density had a signifi-cant effect on the node number and internode length,Liaoning cultivars generally had longer internode length.Plant density had a significant effect on seed yield:stem ratio,as the plant density increased the seed yield:stem ratio decreased for both groups of cultivars.However,100-seed weight was not affected by plant density.
