The binding constant( K b) between fluorescein isothiocyanate labeled bovine serum albumin(FITC BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis(AC...The binding constant( K b) between fluorescein isothiocyanate labeled bovine serum albumin(FITC BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis(ACE) with laser induced fluorescence detection(LIF). Free FITC BSA and the complexes were separated by capillary electrophoresis in 7 min after incubation. The running buffer was 100 mmol/L HEPES at pH 7 4, and separation voltage was 30 kV. The K b was determined to be 4 7×10 8 L/mol using Scatchard plotting method. The influences of incubation time and electrophoresis conditions on determination were also studied.展开更多
采用压力驱动的亲和毛细管电泳技术(P-ACE)分别研究了生理酸度条件下(p H 7.4)3种黄酮类化合物龙血素A、龙血素B和剑叶龙血素C与人血清白蛋白(HSA)之间的相互作用。利用蛋白淌度变化和药物浓度的关系,计算得出上述三者与HSA的结合常数K...采用压力驱动的亲和毛细管电泳技术(P-ACE)分别研究了生理酸度条件下(p H 7.4)3种黄酮类化合物龙血素A、龙血素B和剑叶龙血素C与人血清白蛋白(HSA)之间的相互作用。利用蛋白淌度变化和药物浓度的关系,计算得出上述三者与HSA的结合常数Ka分别为0.414×105,0.252×105,1.816×105L/mol。结果表明,P-ACE可作为研究药物与蛋白相互作用的简便可行方法。展开更多
文摘The binding constant( K b) between fluorescein isothiocyanate labeled bovine serum albumin(FITC BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis(ACE) with laser induced fluorescence detection(LIF). Free FITC BSA and the complexes were separated by capillary electrophoresis in 7 min after incubation. The running buffer was 100 mmol/L HEPES at pH 7 4, and separation voltage was 30 kV. The K b was determined to be 4 7×10 8 L/mol using Scatchard plotting method. The influences of incubation time and electrophoresis conditions on determination were also studied.
文摘采用压力驱动的亲和毛细管电泳技术(P-ACE)分别研究了生理酸度条件下(p H 7.4)3种黄酮类化合物龙血素A、龙血素B和剑叶龙血素C与人血清白蛋白(HSA)之间的相互作用。利用蛋白淌度变化和药物浓度的关系,计算得出上述三者与HSA的结合常数Ka分别为0.414×105,0.252×105,1.816×105L/mol。结果表明,P-ACE可作为研究药物与蛋白相互作用的简便可行方法。
