In this study,PCR product amplified with a RAPD marker A10555 which is linked to gene controlling creeping habit of ground-cover chrysanthemum（Dendranthema grandiflorum） was recovered,and subsequently subcloned and ...In this study,PCR product amplified with a RAPD marker A10555 which is linked to gene controlling creeping habit of ground-cover chrysanthemum（Dendranthema grandiflorum） was recovered,and subsequently subcloned and sequenced. A pair of 18-base specific primers was designed based on the obtained sequence.The validity of this SCAR marker was verified by screening parents and 152 individuals of the F1 progeny,and the consistence in the locus of the special band and the number of recombinants was observed between SCAR analysis and RAPD analysis. The size of this SCAR marker was 555 bp,which was designated as SCA10555. The results demonstrated that the RAPD marker was converted to SCAR marker successfully. This study provides bases for new cultivars developing,molecular markers assisted breeding and creeping habit related genes cloning of the ground-cover chrysanthemum.展开更多
文摘In this study,PCR product amplified with a RAPD marker A10555 which is linked to gene controlling creeping habit of ground-cover chrysanthemum（Dendranthema grandiflorum） was recovered,and subsequently subcloned and sequenced. A pair of 18-base specific primers was designed based on the obtained sequence.The validity of this SCAR marker was verified by screening parents and 152 individuals of the F1 progeny,and the consistence in the locus of the special band and the number of recombinants was observed between SCAR analysis and RAPD analysis. The size of this SCAR marker was 555 bp,which was designated as SCA10555. The results demonstrated that the RAPD marker was converted to SCAR marker successfully. This study provides bases for new cultivars developing,molecular markers assisted breeding and creeping habit related genes cloning of the ground-cover chrysanthemum.
