Effect of hydropuinone (HQ) on rumen urease acivity was studied. Hydroquinone at concentration of 0.01 mg·L -1 ,1 mg·L -1 and 10 mg·L -1 inhibited urease of intact rumen microbes in vitro by...Effect of hydropuinone (HQ) on rumen urease acivity was studied. Hydroquinone at concentration of 0.01 mg·L -1 ,1 mg·L -1 and 10 mg·L -1 inhibited urease of intact rumen microbes in vitro by 25%, 34%, 55% and 64% respectively. In the present of low concentration of βmercaptoethanol,rumen urease could be solubilized and partially purified. The Km for the enzyme was 2×10 -3 mol·L -1 with Vmax of 319.144 μmoles/mg/min.The kinetics of inhibition with partially purified rumen urease was investigated.The result showed that the inhibuitory effect was not eliminated by increasing urea concentration indicating a noncompetitive in nature with inhibition constant 1.2×10 -5 mol·L -1 .Hydropuinone at a concentration that produced 64% urease inhibition did not affect ruminal total dehydrogenase, proteolytic enzyme( P >0.05)but increased cellulase activity by 28%( P <0.05)in vitro.These results demonstrated that hydropuinone was a specific inhibutor of rumen urease and could delay urea hydrolysis effectively without negative effect.The inhibitor appeared to offer the potential to improve nitrogen utilization by ruminants fed diets containing urea.展开更多
Two Co(Ⅱ)and Ni(Ⅱ)complexes were synthesized by synergistic coordination of 3,3-diphenylpropionic acid(HDPA)and 2,2′-bipyridylamine(PAm).The structures of complexes[Co(DPA)_(2)(PAm)]·2H_(2)O(1)and[Ni(DPA)_(2)(...Two Co(Ⅱ)and Ni(Ⅱ)complexes were synthesized by synergistic coordination of 3,3-diphenylpropionic acid(HDPA)and 2,2′-bipyridylamine(PAm).The structures of complexes[Co(DPA)_(2)(PAm)]·2H_(2)O(1)and[Ni(DPA)_(2)(PAm)]·2H_(2)O(2)were determined by single-crystal X-ray diffraction,IR spectroscopy,and powder X-ray diffraction.Hirshfeld surface analysis provided quantitative insights into the intermolecular interactions within the complexes,while molecular docking studies elucidated their binding modes and affinities toward urease.Furthermore,the biological activities of both complexes were systematically evaluated through a range of assays,including DNA binding,urease inhibition,antibacterial activity,and in vitro cytotoxicity against cancer cells.Both complexes exhibited binding affinity for DNA and displayed notable urease inhibitory activity.Under in vitro conditions,both complexes showed appreciable cytotoxicity toward HepG2 cells with efficacy comparable to clinically used platinumbased anticancer agents.CCDC:2479943,1;2479944,2.展开更多
文摘Effect of hydropuinone (HQ) on rumen urease acivity was studied. Hydroquinone at concentration of 0.01 mg·L -1 ,1 mg·L -1 and 10 mg·L -1 inhibited urease of intact rumen microbes in vitro by 25%, 34%, 55% and 64% respectively. In the present of low concentration of βmercaptoethanol,rumen urease could be solubilized and partially purified. The Km for the enzyme was 2×10 -3 mol·L -1 with Vmax of 319.144 μmoles/mg/min.The kinetics of inhibition with partially purified rumen urease was investigated.The result showed that the inhibuitory effect was not eliminated by increasing urea concentration indicating a noncompetitive in nature with inhibition constant 1.2×10 -5 mol·L -1 .Hydropuinone at a concentration that produced 64% urease inhibition did not affect ruminal total dehydrogenase, proteolytic enzyme( P >0.05)but increased cellulase activity by 28%( P <0.05)in vitro.These results demonstrated that hydropuinone was a specific inhibutor of rumen urease and could delay urea hydrolysis effectively without negative effect.The inhibitor appeared to offer the potential to improve nitrogen utilization by ruminants fed diets containing urea.
文摘Two Co(Ⅱ)and Ni(Ⅱ)complexes were synthesized by synergistic coordination of 3,3-diphenylpropionic acid(HDPA)and 2,2′-bipyridylamine(PAm).The structures of complexes[Co(DPA)_(2)(PAm)]·2H_(2)O(1)and[Ni(DPA)_(2)(PAm)]·2H_(2)O(2)were determined by single-crystal X-ray diffraction,IR spectroscopy,and powder X-ray diffraction.Hirshfeld surface analysis provided quantitative insights into the intermolecular interactions within the complexes,while molecular docking studies elucidated their binding modes and affinities toward urease.Furthermore,the biological activities of both complexes were systematically evaluated through a range of assays,including DNA binding,urease inhibition,antibacterial activity,and in vitro cytotoxicity against cancer cells.Both complexes exhibited binding affinity for DNA and displayed notable urease inhibitory activity.Under in vitro conditions,both complexes showed appreciable cytotoxicity toward HepG2 cells with efficacy comparable to clinically used platinumbased anticancer agents.CCDC:2479943,1;2479944,2.
