Sodium cocoyl glycinate(SCG),an environmentally friendly anionic amino acid surfactant,is widely used in daily chemical products as an upgraded alternative to traditional surfactants.In this study,crude Camellia oleif...Sodium cocoyl glycinate(SCG),an environmentally friendly anionic amino acid surfactant,is widely used in daily chemical products as an upgraded alternative to traditional surfactants.In this study,crude Camellia oleifera saponin(COS)was purified using AB-8 macroporous adsorption resin,and its composition and structure were analyzed.The effects of different mole fractions of COS(αCOS)on surface tension(γ),oil-water interfacial tension(IFT),emulsification,and foam properties of COS-SCG binary mixed systems were investigated in mixtures of SCG with purified COS.The stability ofγand foamability under diverse environmental conditions were also discussed.The results indicated that the COS-SCG system exhibited remarkable surface-active synergism.The minimum critical micelle concentration(cmc)of the mixed system was lower than that of SCG,and adding a small mole fraction of COS(1%-2%)induced a synergistic reduction ofγ.Specifically,the cmc andγwere 2.50×10-4 mol/L and 23.1 mN/m forαCOS=1%,respectively.The system exhibited exceptional IFT reduction capacity,achieving a minimum value of 1.42 mN/m atαCOS=10%.The mixed system reached a foaming volume(atαCOS=50%)and foam stability(atαCOS=75%)were 51.0 mL and 97.37%,respectively.Microscopic analysis further confirmed these outstanding foam properties.Moreover,the COS-SCG system displayed reducedγwith enhanced foaming volume under elevated temperatures(35-75℃)and salinity(0-20 g/L).However,acidic conditions and hard water compromised bothγstability and foamability.展开更多
OBJECTIVE To evaluate the synergistic effect of rosmarinic acid(RA)with antibiotics against Staphylococcus aureus(S.aureus)and MRSA and to identify the possible mechanism of action responsible for synergism if any.MET...OBJECTIVE To evaluate the synergistic effect of rosmarinic acid(RA)with antibiotics against Staphylococcus aureus(S.aureus)and MRSA and to identify the possible mechanism of action responsible for synergism if any.METHODS The antibacterial activity of Rosmarinic acid was studied for its zone of inhibition by agar well diffusion and MIC determination by liquid broth dilution technique against MRSA and VRSA.The synergistic effect of RA with antibiotics like amoxicillin,ofloxacin and vancomycin was evaluated by Broth checker board method and further confirmed with time kill kinetic studies.Microbial Surface Components Recognizing Adhesive Matrix Molecules(MSCRAMMs)were isolated by protein precipitation technique and its expression was studied by SDS PAGE.Further RA was evaluated for its beta lactamase inhibition property.RESULTS Rosmarinic acid exhibited antibacterial activity against S.aureus and MRSAby showing a MIC value of 0.8mg·mL-1 against S.aureus and 10mg·mL-1 against MRSA.Rosmarinic acid at 1/4X MIC value reduced the MIC of vancomycin,amoxicillin and ofloxacin by 1/4 times against S.aureus.But against MRSA,vancomycin was found to be synergistic.All the synergistic combinations have shown FIC value of 0.5.In order to measure the kinetics of the anti-bacterial activity,the bacterial growth rate with RA,antibiotics and synergistic combinations against S.aureus and MRSA was studied.It is observed that the synergistic combinations showed better time kill kinetics as compared to RA and antibiotics.Further RA could able to destruct the cell surface proteins MSCRAMMs which was studied by SDS PAGE.RA was also found to showβlactamase inhibiting property.CONCLUSION It is concluded that RA possess antibacterial activity but at a very higher concentration(in mg/mL)against S.aureus and MRSA.However it shows synergism with antibiotics and could able to reduce the MIC of antibiotics.Thus RA could be developed as an adjuvant for antibiotics against S.aureus and MRSA caused infections.Further studies are needed to identify the mechanism for its synergism with antibiotics.展开更多
Effects of -carboxyethylgermanium sesquioxide (Ge-132) on the function of antioxidative system of cow and the relationship among autioxidative elements Ge, selenium (Se), copper(Cu) and zinc(Zn) were studied for thre...Effects of -carboxyethylgermanium sesquioxide (Ge-132) on the function of antioxidative system of cow and the relationship among autioxidative elements Ge, selenium (Se), copper(Cu) and zinc(Zn) were studied for three months. 25 cow fed on the same basal diet were randomly divided into 5 groups, 5 for each group. Group was used as the control. Investigated elements were administered to the following animals, group : Ge132;Group :Ge-132 and Se; Group : Ge-132, Se and Zn; Group : Ge-132, Se,Zn and Cu. Blood samples were collected at regular intervals to examine the activities of serum GSH-Px,CuZn-SOD, and the contents of serum MDA, and Ge, Se, Zn, Cu in whole blood. The results indicated that the activities of serum GSH-Px and CuZn-SOD in group , , were significantly higher than those in group (P< 001),but the content of serum MDA significantly lower( P <001). There was a significantly positive correlation between the content of blood Ge and the activities of serum GSH-Px, Cu Zn-SOD(r=04997 and r=02502), but a negative correlation between the content of blood Ge and serum MDA(r=02996). The activity of serum GSH-Px in group was significantly higher than that in group . There was a significantly positive correlation be tween the content of blood Ge and Se. It can be concluded that Ge-132 and Se synergically played an important role in resisting peroxidation and scanvenging free radicals in cow.展开更多
文摘Sodium cocoyl glycinate(SCG),an environmentally friendly anionic amino acid surfactant,is widely used in daily chemical products as an upgraded alternative to traditional surfactants.In this study,crude Camellia oleifera saponin(COS)was purified using AB-8 macroporous adsorption resin,and its composition and structure were analyzed.The effects of different mole fractions of COS(αCOS)on surface tension(γ),oil-water interfacial tension(IFT),emulsification,and foam properties of COS-SCG binary mixed systems were investigated in mixtures of SCG with purified COS.The stability ofγand foamability under diverse environmental conditions were also discussed.The results indicated that the COS-SCG system exhibited remarkable surface-active synergism.The minimum critical micelle concentration(cmc)of the mixed system was lower than that of SCG,and adding a small mole fraction of COS(1%-2%)induced a synergistic reduction ofγ.Specifically,the cmc andγwere 2.50×10-4 mol/L and 23.1 mN/m forαCOS=1%,respectively.The system exhibited exceptional IFT reduction capacity,achieving a minimum value of 1.42 mN/m atαCOS=10%.The mixed system reached a foaming volume(atαCOS=50%)and foam stability(atαCOS=75%)were 51.0 mL and 97.37%,respectively.Microscopic analysis further confirmed these outstanding foam properties.Moreover,the COS-SCG system displayed reducedγwith enhanced foaming volume under elevated temperatures(35-75℃)and salinity(0-20 g/L).However,acidic conditions and hard water compromised bothγstability and foamability.
基金The project supported by TEQIP PhaseⅡgrant under Anna University BIT campus,Tiruchirappalli-620024,Tamilnadu,India
文摘OBJECTIVE To evaluate the synergistic effect of rosmarinic acid(RA)with antibiotics against Staphylococcus aureus(S.aureus)and MRSA and to identify the possible mechanism of action responsible for synergism if any.METHODS The antibacterial activity of Rosmarinic acid was studied for its zone of inhibition by agar well diffusion and MIC determination by liquid broth dilution technique against MRSA and VRSA.The synergistic effect of RA with antibiotics like amoxicillin,ofloxacin and vancomycin was evaluated by Broth checker board method and further confirmed with time kill kinetic studies.Microbial Surface Components Recognizing Adhesive Matrix Molecules(MSCRAMMs)were isolated by protein precipitation technique and its expression was studied by SDS PAGE.Further RA was evaluated for its beta lactamase inhibition property.RESULTS Rosmarinic acid exhibited antibacterial activity against S.aureus and MRSAby showing a MIC value of 0.8mg·mL-1 against S.aureus and 10mg·mL-1 against MRSA.Rosmarinic acid at 1/4X MIC value reduced the MIC of vancomycin,amoxicillin and ofloxacin by 1/4 times against S.aureus.But against MRSA,vancomycin was found to be synergistic.All the synergistic combinations have shown FIC value of 0.5.In order to measure the kinetics of the anti-bacterial activity,the bacterial growth rate with RA,antibiotics and synergistic combinations against S.aureus and MRSA was studied.It is observed that the synergistic combinations showed better time kill kinetics as compared to RA and antibiotics.Further RA could able to destruct the cell surface proteins MSCRAMMs which was studied by SDS PAGE.RA was also found to showβlactamase inhibiting property.CONCLUSION It is concluded that RA possess antibacterial activity but at a very higher concentration(in mg/mL)against S.aureus and MRSA.However it shows synergism with antibiotics and could able to reduce the MIC of antibiotics.Thus RA could be developed as an adjuvant for antibiotics against S.aureus and MRSA caused infections.Further studies are needed to identify the mechanism for its synergism with antibiotics.
文摘Effects of -carboxyethylgermanium sesquioxide (Ge-132) on the function of antioxidative system of cow and the relationship among autioxidative elements Ge, selenium (Se), copper(Cu) and zinc(Zn) were studied for three months. 25 cow fed on the same basal diet were randomly divided into 5 groups, 5 for each group. Group was used as the control. Investigated elements were administered to the following animals, group : Ge132;Group :Ge-132 and Se; Group : Ge-132, Se and Zn; Group : Ge-132, Se,Zn and Cu. Blood samples were collected at regular intervals to examine the activities of serum GSH-Px,CuZn-SOD, and the contents of serum MDA, and Ge, Se, Zn, Cu in whole blood. The results indicated that the activities of serum GSH-Px and CuZn-SOD in group , , were significantly higher than those in group (P< 001),but the content of serum MDA significantly lower( P <001). There was a significantly positive correlation between the content of blood Ge and the activities of serum GSH-Px, Cu Zn-SOD(r=04997 and r=02502), but a negative correlation between the content of blood Ge and serum MDA(r=02996). The activity of serum GSH-Px in group was significantly higher than that in group . There was a significantly positive correlation be tween the content of blood Ge and Se. It can be concluded that Ge-132 and Se synergically played an important role in resisting peroxidation and scanvenging free radicals in cow.
