In order to explore the salt tolerance mechanism of Bacillus cereus LBR-4 with salinity of 14%NaCl,differential proteomic analysis of the whole protein of LBR-4 strain expressed under 14%NaCl high salinity condition a...In order to explore the salt tolerance mechanism of Bacillus cereus LBR-4 with salinity of 14%NaCl,differential proteomic analysis of the whole protein of LBR-4 strain expressed under 14%NaCl high salinity condition and normalculture condition(1%NaCl)was studied by two-dimensional electrophoresis and mass spectrometry.The isoelectric point of most detected proteins was between pH 4-7 and the molecular weight distribution was 10-70 ku.Compared with the normal culture condition,the expression level of 118 protein spots in the whole protein expression map changed significantly(accounting for 25.2%of the total protein spots).The expression level of 78 protein spots increased significantly,including 22 new protein spots that appeared under high salt stress.The expression levels of 40 protein spots decreased significantly,including 18 protein spots that disappeared under high salt stress.By mass spectrometry,six distinct differentially expressed protein spotswere dihydroxy acid dehydratase,cell division protein FtsZ,iron sulfur cluster synthesis protein SufD,unknown carboxylase YngE,hypothetical acetaldehyde dehydrogenase DhaS and phenylalanine acid tRNA ligase alpha subunit.It was speculated that under high salt stress,the cells had protective measures and the secretion of intracellular compatible solutes increased.The iron and sulfur clusters involved in various physiological reactions also activated the stressful suf synthesis pathway,and therate of cell division and reproduction was also slowed down and ensured the normal progress of physiological reactions inthe cells.展开更多
Background:Salinity is a major abiotic stress to global agriculture which hampers crop growth and development,and eventually reduces yield.Transgenic technology is an e ective and e cient approach to improve crop salt...Background:Salinity is a major abiotic stress to global agriculture which hampers crop growth and development,and eventually reduces yield.Transgenic technology is an e ective and e cient approach to improve crop salt tolerance but depending on the availability of e ective genes.We previously isolated Salt Tolerance5(ThST5)from the halophyte Thellungiella halophila,an ortholog of Arabidopsis SPT4-2 which encodes a transcription elongation factor.However,SPT4-2-confered salt tolerance has not been evaluated in crops yet.Here we report the evaluation of Th ST5-conferred salt tolerance in cotton(Gossypium hirsutum L.).Results:The ThST5 overexpression transgenic cotton plants displayed enhanced tolerance to salt stress during seed germination and seedling stage compared with wild type.Particularly,the transgenic plants showed improved salinity tolerance as well as yield under saline field conditions.Comparative transcriptomic analysis showed that ThST5 improved salt tolerance of transgenic cotton mainly by maintaining ion homeostasis.In addition,ThST5 also orchestrated the expression of genes encoding antioxidants and salt-responsive transcription factors.Conclusion:Our results demonstrate that ThST5 is a promising candidate to improve salt tolerance in cotton.展开更多
Background: Gossypium hirsutum(upland cotton) is one of the principal fiber crops in the world. Cotton yield is highly affected by abiotic stresses, among which salt stress is considered as a major problem around the ...Background: Gossypium hirsutum(upland cotton) is one of the principal fiber crops in the world. Cotton yield is highly affected by abiotic stresses, among which salt stress is considered as a major problem around the globe. Transgenic approach is efficient to improve cotton salt tolerance but depending on the availability of salt tolerance genes.Results: In this study we evaluated salt tolerance candidate gene ST7 from Thellungiella halophila, encoding a homolog of Arabidopsis aluminum-induced protein, in cotton. Our results showed that ThST7 overexpression in cotton improved germination under NaCl stress as well as seedling growth. Our field trials also showed that ThST7 transgenic cotton lines produced higher yield under salt stress conditions. The improved salt tolerance of the transgenic cotton lines was partially contributed by enhanced antioxidation as shown by diaminobenzidine(DAB) and nitrotetrazolium blue chloride(NBT) staining. Moreover, transcriptomic analysis of ThST7 overexpression lines showed a significant upregulation of the genes involved in ion homeostasis and antioxidation, consistent with the salt tolerance phenotype of the transgenic cotton.Conclusions: Our results demonstrate that ThST7 has the ability to improve salt tolerance in cotton. The ThST7 transgenic cotton may be used in cotton breeding for salt tolerance cultivars.展开更多
基金Supported by Heilongjiang Province National Science Foundation(LH2020C007)。
文摘In order to explore the salt tolerance mechanism of Bacillus cereus LBR-4 with salinity of 14%NaCl,differential proteomic analysis of the whole protein of LBR-4 strain expressed under 14%NaCl high salinity condition and normalculture condition(1%NaCl)was studied by two-dimensional electrophoresis and mass spectrometry.The isoelectric point of most detected proteins was between pH 4-7 and the molecular weight distribution was 10-70 ku.Compared with the normal culture condition,the expression level of 118 protein spots in the whole protein expression map changed significantly(accounting for 25.2%of the total protein spots).The expression level of 78 protein spots increased significantly,including 22 new protein spots that appeared under high salt stress.The expression levels of 40 protein spots decreased significantly,including 18 protein spots that disappeared under high salt stress.By mass spectrometry,six distinct differentially expressed protein spotswere dihydroxy acid dehydratase,cell division protein FtsZ,iron sulfur cluster synthesis protein SufD,unknown carboxylase YngE,hypothetical acetaldehyde dehydrogenase DhaS and phenylalanine acid tRNA ligase alpha subunit.It was speculated that under high salt stress,the cells had protective measures and the secretion of intracellular compatible solutes increased.The iron and sulfur clusters involved in various physiological reactions also activated the stressful suf synthesis pathway,and therate of cell division and reproduction was also slowed down and ensured the normal progress of physiological reactions inthe cells.
基金supported by grants from the Ministry of Science and Technol-ogy of China(Grant No.2016ZX08005004-003).
文摘Background:Salinity is a major abiotic stress to global agriculture which hampers crop growth and development,and eventually reduces yield.Transgenic technology is an e ective and e cient approach to improve crop salt tolerance but depending on the availability of e ective genes.We previously isolated Salt Tolerance5(ThST5)from the halophyte Thellungiella halophila,an ortholog of Arabidopsis SPT4-2 which encodes a transcription elongation factor.However,SPT4-2-confered salt tolerance has not been evaluated in crops yet.Here we report the evaluation of Th ST5-conferred salt tolerance in cotton(Gossypium hirsutum L.).Results:The ThST5 overexpression transgenic cotton plants displayed enhanced tolerance to salt stress during seed germination and seedling stage compared with wild type.Particularly,the transgenic plants showed improved salinity tolerance as well as yield under saline field conditions.Comparative transcriptomic analysis showed that ThST5 improved salt tolerance of transgenic cotton mainly by maintaining ion homeostasis.In addition,ThST5 also orchestrated the expression of genes encoding antioxidants and salt-responsive transcription factors.Conclusion:Our results demonstrate that ThST5 is a promising candidate to improve salt tolerance in cotton.
基金supported by grants from Ministry of Science and Technology of China(Grant No.2016ZX08005004-003).
文摘Background: Gossypium hirsutum(upland cotton) is one of the principal fiber crops in the world. Cotton yield is highly affected by abiotic stresses, among which salt stress is considered as a major problem around the globe. Transgenic approach is efficient to improve cotton salt tolerance but depending on the availability of salt tolerance genes.Results: In this study we evaluated salt tolerance candidate gene ST7 from Thellungiella halophila, encoding a homolog of Arabidopsis aluminum-induced protein, in cotton. Our results showed that ThST7 overexpression in cotton improved germination under NaCl stress as well as seedling growth. Our field trials also showed that ThST7 transgenic cotton lines produced higher yield under salt stress conditions. The improved salt tolerance of the transgenic cotton lines was partially contributed by enhanced antioxidation as shown by diaminobenzidine(DAB) and nitrotetrazolium blue chloride(NBT) staining. Moreover, transcriptomic analysis of ThST7 overexpression lines showed a significant upregulation of the genes involved in ion homeostasis and antioxidation, consistent with the salt tolerance phenotype of the transgenic cotton.Conclusions: Our results demonstrate that ThST7 has the ability to improve salt tolerance in cotton. The ThST7 transgenic cotton may be used in cotton breeding for salt tolerance cultivars.
