The content of flavones of the seabuckthorn ( Hippophae rhamnoides L. )root bark,branch bark and leaves were determined using UV vis spectrophotometry.In the meantime,the scavenging hydroxyl free radical(OH ·)and...The content of flavones of the seabuckthorn ( Hippophae rhamnoides L. )root bark,branch bark and leaves were determined using UV vis spectrophotometry.In the meantime,the scavenging hydroxyl free radical(OH ·)and superoxide anion free radical(O 2 ·- )of the extracts of seabuckthorn root bark,branch bark and leaves were measured through Fenton reaction and pyrogallol autoxidation method.The results showed that the seabuckthorn root bark,branch bark and leaves had a strong capacity on scavenging free radical.The scavenging rates of hydroxyl free radical were 69.2%,62.2% and 74.1%,respectively.The inhibition rates of superoxide anion free radical were 10.5%,12.3% and 33.3%,respectively.Finally the antioxidant mechanism of seabuckthorn flavones were tentatively explored.展开更多
The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 ad...The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 adducts have an excellent efficiency in eliminating superoxide radical generated from auto oxidation of pyrogallol. At a mass concentration level of 150 μg/mL for β alanine C 60 adducts, the radical scavenging efficiency is approximately equivalent to one enzyme unit, in vitro experiments show that β alanine C 60 adducts can enhance the activity of mouse thymus cells and prolong their life time span. It reveals the potential use of these β alanine C 60 adducts as the novel potent free radical scavengers in biological systems.展开更多
首次实验研究了中华芦荟(Aloe vera var.chinensis(Haw)Berg.)植物对甲醛的吸收以及植物体内超氧化物歧化酶(SOD)活性的变化情况,旨在了解芦荟净化室内甲醛气体的生理机制。实验以玻璃箱模拟室内空间环境,用乙酰丙酮分光光度法测定模拟...首次实验研究了中华芦荟(Aloe vera var.chinensis(Haw)Berg.)植物对甲醛的吸收以及植物体内超氧化物歧化酶(SOD)活性的变化情况,旨在了解芦荟净化室内甲醛气体的生理机制。实验以玻璃箱模拟室内空间环境,用乙酰丙酮分光光度法测定模拟空间内甲醛的浓度,用邻苯三酚自氧化法测定SOD的活性。结果表明,有芦荟存在的条件下,模拟空间内甲醛气体浓度显著下降。随着通入甲醛量的增加,芦荟对甲醛的吸收有着较显著的增加,并且SOD酶活性的变化也越来越显著。芦荟体内SOD酶对甲醛气体的胁迫存在着一定程度的生理应激反应,芦荟对室内甲醛污染存在一定程度的净化作用。展开更多
文摘The content of flavones of the seabuckthorn ( Hippophae rhamnoides L. )root bark,branch bark and leaves were determined using UV vis spectrophotometry.In the meantime,the scavenging hydroxyl free radical(OH ·)and superoxide anion free radical(O 2 ·- )of the extracts of seabuckthorn root bark,branch bark and leaves were measured through Fenton reaction and pyrogallol autoxidation method.The results showed that the seabuckthorn root bark,branch bark and leaves had a strong capacity on scavenging free radical.The scavenging rates of hydroxyl free radical were 69.2%,62.2% and 74.1%,respectively.The inhibition rates of superoxide anion free radical were 10.5%,12.3% and 33.3%,respectively.Finally the antioxidant mechanism of seabuckthorn flavones were tentatively explored.
文摘The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 adducts have an excellent efficiency in eliminating superoxide radical generated from auto oxidation of pyrogallol. At a mass concentration level of 150 μg/mL for β alanine C 60 adducts, the radical scavenging efficiency is approximately equivalent to one enzyme unit, in vitro experiments show that β alanine C 60 adducts can enhance the activity of mouse thymus cells and prolong their life time span. It reveals the potential use of these β alanine C 60 adducts as the novel potent free radical scavengers in biological systems.
文摘首次实验研究了中华芦荟(Aloe vera var.chinensis(Haw)Berg.)植物对甲醛的吸收以及植物体内超氧化物歧化酶(SOD)活性的变化情况,旨在了解芦荟净化室内甲醛气体的生理机制。实验以玻璃箱模拟室内空间环境,用乙酰丙酮分光光度法测定模拟空间内甲醛的浓度,用邻苯三酚自氧化法测定SOD的活性。结果表明,有芦荟存在的条件下,模拟空间内甲醛气体浓度显著下降。随着通入甲醛量的增加,芦荟对甲醛的吸收有着较显著的增加,并且SOD酶活性的变化也越来越显著。芦荟体内SOD酶对甲醛气体的胁迫存在着一定程度的生理应激反应,芦荟对室内甲醛污染存在一定程度的净化作用。