Although white clover has been widely studied and applied,less is known about its physiological responses to chilling stress in northern China.The effects of acclimation time of chilling stress in the two white clover...Although white clover has been widely studied and applied,less is known about its physiological responses to chilling stress in northern China.The effects of acclimation time of chilling stress in the two white clover cultivars,Rivendel and Haifa,after exposure to 4℃for 0,4,8,12,24 and 48 h were investigated.Seven physiological indexes were measured in shoots of the two cultivars.It was found that Rivendel had a strong ability to maintain the stability of cells,cope with oxidative damage and accumulate osmotic adjustment substances compared with Haifa.In addition,a comprehensive evaluation on the chilling tolerance of the two cultivars based on subordinate function value analysis showed a stronger chilling tolerance of Rivendel than Haifa.展开更多
The effects of 6-benzylaminopurine （6-BA） on several physiological indexes of Caryota mitis and C. obtusa seedlings treated at varied temperatures were conducted, the leaves of the two palm species were sprayed with...The effects of 6-benzylaminopurine （6-BA） on several physiological indexes of Caryota mitis and C. obtusa seedlings treated at varied temperatures were conducted, the leaves of the two palm species were sprayed with 0, 25, 50 and 100 mg · L1 6-BA solutions, the activities of superoxide dismutase （SOD）, peroxidase （POD）, and the contents of malondialdehyde （MDA）, proline, soluble protein, chlorophyll and chlorophyll a/b were determined under 25 ℃, 5 ℃ and 25 ℃ for 24 h, respectively. The dynamics of a series of important physiological components derived from the leaves were studied. It was indicated that all the above mentioned physiological indexes varied with different concentrations of 6-BA and temperature. The fuzzy comprehensive evaluation values for different concentrations of 6-BA at the recovery temperature 25℃ were 0.33, 0.28, 0.92 and 0.33, respectively for C. mitis, and 0.20, 0.49, 0.56 and 0.63, respectively for C. obtusa. It was concluded that leaves sprayed with different concentrations of 6-BA could affect the cold tolerance of palm seedlings. The optimal concentration of 6-BA was different for different palm species.展开更多
In order to study the alkali resistivity of VHA-c in Iris lacteal. （IrlVHA-c）, the transgenic tobacco seedlings harboring lrlVHA-c gene from Iris lactea （To）, the self-crossed progeny （T1）, and the non-transgeni...In order to study the alkali resistivity of VHA-c in Iris lacteal. （IrlVHA-c）, the transgenic tobacco seedlings harboring lrlVHA-c gene from Iris lactea （To）, the self-crossed progeny （T1）, and the non-transgenic lines of tobacco seedlings were grown in Hoagland nutrient solutions supplemented with 0, 100, 200, 300, 400 mmol.L^-1 NaHCO3. The MDA content, CAT, POD and SOD activity, electrical conductivity, chlorophyll content, soluble sugar content, proline content and polyphenol oxidase activity of the seedlings were determined. The results showed that the transgenic lines of tobacco maintained a high activity up to 200 mmol.L^-1 NaHCO3, and activity was slightly lower at 300 mmol.L^-1 NaHCOa. When the concentration of NaHCO3 was as high as 400 mmol.L^-1 the seedlings were badly hurt. In addition, the activity of To and T1 transgentic tobacoo was maintained more or less. While the non- transgenic lines of tobacco could maintain viably up to 100 mmol. L^-1 NaHCO3, and they could not survive at 400 mmol. L^-1 NaHCO3. The conclusion was drawn that the alkali resistance of the tobacoo transformed IrlVHA-c was noticeably improved.展开更多
基金Supported by the National Natural Science Foundation of China(31802120)the Natural Science Foundation of Heilongjiang Province of China(LH2021C040)Academic Backbone Fund Project of Northeast Agricultural University。
文摘Although white clover has been widely studied and applied,less is known about its physiological responses to chilling stress in northern China.The effects of acclimation time of chilling stress in the two white clover cultivars,Rivendel and Haifa,after exposure to 4℃for 0,4,8,12,24 and 48 h were investigated.Seven physiological indexes were measured in shoots of the two cultivars.It was found that Rivendel had a strong ability to maintain the stability of cells,cope with oxidative damage and accumulate osmotic adjustment substances compared with Haifa.In addition,a comprehensive evaluation on the chilling tolerance of the two cultivars based on subordinate function value analysis showed a stronger chilling tolerance of Rivendel than Haifa.
基金Supported by Fund of Xiamen Municipal Science&Technology Commision(3502Z20092019)
文摘The effects of 6-benzylaminopurine （6-BA） on several physiological indexes of Caryota mitis and C. obtusa seedlings treated at varied temperatures were conducted, the leaves of the two palm species were sprayed with 0, 25, 50 and 100 mg · L1 6-BA solutions, the activities of superoxide dismutase （SOD）, peroxidase （POD）, and the contents of malondialdehyde （MDA）, proline, soluble protein, chlorophyll and chlorophyll a/b were determined under 25 ℃, 5 ℃ and 25 ℃ for 24 h, respectively. The dynamics of a series of important physiological components derived from the leaves were studied. It was indicated that all the above mentioned physiological indexes varied with different concentrations of 6-BA and temperature. The fuzzy comprehensive evaluation values for different concentrations of 6-BA at the recovery temperature 25℃ were 0.33, 0.28, 0.92 and 0.33, respectively for C. mitis, and 0.20, 0.49, 0.56 and 0.63, respectively for C. obtusa. It was concluded that leaves sprayed with different concentrations of 6-BA could affect the cold tolerance of palm seedlings. The optimal concentration of 6-BA was different for different palm species.
基金Supported by the Post-doctoral research start-up funding of Heilongjiang Province (LBH-210144)Postdoctoral Funds Project of Heilongjiang Province (520-415037)
文摘In order to study the alkali resistivity of VHA-c in Iris lacteal. （IrlVHA-c）, the transgenic tobacco seedlings harboring lrlVHA-c gene from Iris lactea （To）, the self-crossed progeny （T1）, and the non-transgenic lines of tobacco seedlings were grown in Hoagland nutrient solutions supplemented with 0, 100, 200, 300, 400 mmol.L^-1 NaHCO3. The MDA content, CAT, POD and SOD activity, electrical conductivity, chlorophyll content, soluble sugar content, proline content and polyphenol oxidase activity of the seedlings were determined. The results showed that the transgenic lines of tobacco maintained a high activity up to 200 mmol.L^-1 NaHCO3, and activity was slightly lower at 300 mmol.L^-1 NaHCOa. When the concentration of NaHCO3 was as high as 400 mmol.L^-1 the seedlings were badly hurt. In addition, the activity of To and T1 transgentic tobacoo was maintained more or less. While the non- transgenic lines of tobacco could maintain viably up to 100 mmol. L^-1 NaHCO3, and they could not survive at 400 mmol. L^-1 NaHCO3. The conclusion was drawn that the alkali resistance of the tobacoo transformed IrlVHA-c was noticeably improved.
