This paper reported the identification of the primary structure of two peptides using nanoelectrospray tandem mass spectrometry(Nano-ESI MS/MS).Firstly,the relative molecular mass of two peptides were determined in ES...This paper reported the identification of the primary structure of two peptides using nanoelectrospray tandem mass spectrometry(Nano-ESI MS/MS).Firstly,the relative molecular mass of two peptides were determined in ESI-TOF MS mode.Then fragmentation ions were obtained by selecting [M+2H]2+ ion using tandem mass spectrometry(MS/MS).Finally,the primary structure of triptorelin is determined to be E’HWSYWLRPG’,of which the N-terminal is pyroglutamic acid (E’) and the C-termianl is glycinamide (G’).The primary structure of unknown peptide was identified to be T’VSP VWLPPSVY by sequence docking method,of which the N-terminal occurs threonine phosphorylation (T’) and the foruth proline is added with sodium ion (P).The data suggested that electrospray Tandem mass spectrometry technique have obvious advantage in analyzing the full sequence of modified or unknown peptides.展开更多
文摘This paper reported the identification of the primary structure of two peptides using nanoelectrospray tandem mass spectrometry(Nano-ESI MS/MS).Firstly,the relative molecular mass of two peptides were determined in ESI-TOF MS mode.Then fragmentation ions were obtained by selecting [M+2H]2+ ion using tandem mass spectrometry(MS/MS).Finally,the primary structure of triptorelin is determined to be E’HWSYWLRPG’,of which the N-terminal is pyroglutamic acid (E’) and the C-termianl is glycinamide (G’).The primary structure of unknown peptide was identified to be T’VSP VWLPPSVY by sequence docking method,of which the N-terminal occurs threonine phosphorylation (T’) and the foruth proline is added with sodium ion (P).The data suggested that electrospray Tandem mass spectrometry technique have obvious advantage in analyzing the full sequence of modified or unknown peptides.
