A model of high altitude cerebral hypoxia was made by two weeks' exposure to simulated high altitude of 5000 min 48 Wistar rats.The levels of Na+ -K+ -ATPase and Na+,K+ and water contents in the brain cortex were ...A model of high altitude cerebral hypoxia was made by two weeks' exposure to simulated high altitude of 5000 min 48 Wistar rats.The levels of Na+ -K+ -ATPase and Na+,K+ and water contents in the brain cortex were measured. The changes of neuronal ultrastructure were revealed with transmission electron microscope (TEM)examination. The results showed that the activity of Na+ -K+ -ATPase in the cerebral cortex reduced significantly (P<0. 01).The contents of Na+ and water in the cerebral cortex increased evidently (P<0.01).The damage of cortical neurons and neuronal swelling were very obvious under TEM.The experimental findings suggest that the depression of Na+-K+-ATPase activity and metabolic disorders of Na+ and K+ in the brain tissue might be an important factor for the occurrence and development of high altitude cerebral edema.展开更多
Somatasensory evoked potentials (SEP), sciatic nerve conduction velocity (CV), sciatic nerve Na+-K+-ATPase activity, and lipid peroxide content (LPO) were determined in 8 rats with chronic renal failure induced with 5...Somatasensory evoked potentials (SEP), sciatic nerve conduction velocity (CV), sciatic nerve Na+-K+-ATPase activity, and lipid peroxide content (LPO) were determined in 8 rats with chronic renal failure induced with 5/6 nephrectomy. Meanwhile, the histological changes were also oserved. Ten rats wtih sham operation served as the control. It was found that in the group with chronic renal failure, the mean peak latency of SEP was significantly prolonged and CV slower than those of the control. In the sciatic nerve tissue, Na+-K+-ATPase activity was loyer and LPO content higher in the tested group than in the control. No significant histological changes of the sciatic nerve were found under optical microscope. These findings suggest that the decrease of nerve conductivity after chronic renal failure might result from the insufficient activity of Na+-K+-ATPase and enhanced generation of free oxygen radicals.展开更多
文摘A model of high altitude cerebral hypoxia was made by two weeks' exposure to simulated high altitude of 5000 min 48 Wistar rats.The levels of Na+ -K+ -ATPase and Na+,K+ and water contents in the brain cortex were measured. The changes of neuronal ultrastructure were revealed with transmission electron microscope (TEM)examination. The results showed that the activity of Na+ -K+ -ATPase in the cerebral cortex reduced significantly (P<0. 01).The contents of Na+ and water in the cerebral cortex increased evidently (P<0.01).The damage of cortical neurons and neuronal swelling were very obvious under TEM.The experimental findings suggest that the depression of Na+-K+-ATPase activity and metabolic disorders of Na+ and K+ in the brain tissue might be an important factor for the occurrence and development of high altitude cerebral edema.
文摘Somatasensory evoked potentials (SEP), sciatic nerve conduction velocity (CV), sciatic nerve Na+-K+-ATPase activity, and lipid peroxide content (LPO) were determined in 8 rats with chronic renal failure induced with 5/6 nephrectomy. Meanwhile, the histological changes were also oserved. Ten rats wtih sham operation served as the control. It was found that in the group with chronic renal failure, the mean peak latency of SEP was significantly prolonged and CV slower than those of the control. In the sciatic nerve tissue, Na+-K+-ATPase activity was loyer and LPO content higher in the tested group than in the control. No significant histological changes of the sciatic nerve were found under optical microscope. These findings suggest that the decrease of nerve conductivity after chronic renal failure might result from the insufficient activity of Na+-K+-ATPase and enhanced generation of free oxygen radicals.
