Manganese(Mn),an essential trace element in the human body,plays critical roles in many biological processes.Recent studies have discovered that Mn^(2+)may promote or directly activate the cGAS-STING pathway,thereby s...Manganese(Mn),an essential trace element in the human body,plays critical roles in many biological processes.Recent studies have discovered that Mn^(2+)may promote or directly activate the cGAS-STING pathway,thereby subsequently initiating the natural immune response and augmenting antitumor therapy.However,the current lack of accurate methods for Mn^(2+)determination in cells significantly limits their mechanism investigation;hence,it is urgent to establish novel tools to detect Mn^(2+)in cells.In this study,the dual-emission carbon dots were initially synthesized via the one-pot hydrothermal method employing L-aspartic acid and p-phenylenediamine as raw materials.In the presence of Mn^(2+),the emission peak centered at 350 nm exhibited significant enhancement,whereas another peak at 610 nm remained stable.Consequently,a ratiometric sensor for Mn^(2+)determination was established using the signal at 350 nm as the responsive signal and the signal at 610 nm as an internal reference.Under the optimal condition,a good linear relationship was achieved between the F350/F610 value and Mn^(2+)concentration ranging from 0.9 to 15μmol/L,with a calculated LOD of 61 nmol/L.Benefiting from the special Mn^(2+)-induced ratiometric approach,this method demonstrates outstanding sensitivity,selectivity,and stability,rendering it applicable for Mn^(2+)determination in complex biological samples,as well as Mn^(2+)imaging in MKN-45 and LO2 cells.展开更多
基金Supported by National Natural Science Foundation of China(22264023)Natural Science Foundation of Shaanxi Province(2024JC-YBQN-0150)+2 种基金Yan'an Science and Technology Bureau Project(2023-SFGG-057)Scientific Research Projects of Education Department of Shaanxi Province(22JK0614)PhD Start Fund of Yan'an University(YDBK2022-15)。
文摘Manganese(Mn),an essential trace element in the human body,plays critical roles in many biological processes.Recent studies have discovered that Mn^(2+)may promote or directly activate the cGAS-STING pathway,thereby subsequently initiating the natural immune response and augmenting antitumor therapy.However,the current lack of accurate methods for Mn^(2+)determination in cells significantly limits their mechanism investigation;hence,it is urgent to establish novel tools to detect Mn^(2+)in cells.In this study,the dual-emission carbon dots were initially synthesized via the one-pot hydrothermal method employing L-aspartic acid and p-phenylenediamine as raw materials.In the presence of Mn^(2+),the emission peak centered at 350 nm exhibited significant enhancement,whereas another peak at 610 nm remained stable.Consequently,a ratiometric sensor for Mn^(2+)determination was established using the signal at 350 nm as the responsive signal and the signal at 610 nm as an internal reference.Under the optimal condition,a good linear relationship was achieved between the F350/F610 value and Mn^(2+)concentration ranging from 0.9 to 15μmol/L,with a calculated LOD of 61 nmol/L.Benefiting from the special Mn^(2+)-induced ratiometric approach,this method demonstrates outstanding sensitivity,selectivity,and stability,rendering it applicable for Mn^(2+)determination in complex biological samples,as well as Mn^(2+)imaging in MKN-45 and LO2 cells.
