基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生...基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生物实习带教带来了新的挑战。本文阐述了MALDI-TOF MS技术在临床微生物检验实习带教中的应用探讨,旨在充分运用MALDI-TOF MS技术结合传统鉴定技术,提高临床微生物检验实习的教学能力。展开更多
CHCA as a matrix facilitates the ionization of proteins and peptides in a matrix-assisted laser desorption/ionization(MALDI) time-of-flight(TOF) mass spectrometer. The matrix itself also ionizes and so do its adducts....CHCA as a matrix facilitates the ionization of proteins and peptides in a matrix-assisted laser desorption/ionization(MALDI) time-of-flight(TOF) mass spectrometer. The matrix itself also ionizes and so do its adducts. Matrix clusters and adducts interfere with peptide ionization and peptide mass spectrum interpretation, especially at low sample concentrations and within m/z 500-1 000 range. These matrix adducts, especially within m/z 500-1 000, are significantly reduced with addition of ammonium citrate, ammonium sulfate, ammonium benzoate, ammonium bicarbonate, ammonium citrate and potassium dihydrogen phosphate to the matrix and the samples were deposited onto the MALDI target. The reduction of matrix adducts improves peptide ionization and signal-to-noise ratio, which enhances the reliability of database searching.展开更多
文摘基质辅助激光解吸电离飞行时间质谱(Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术是临床微生物诊断中的一项革命性创新技术,给临床微生物诊断带来了极大的便利,同时也给临床微生物实习带教带来了新的挑战。本文阐述了MALDI-TOF MS技术在临床微生物检验实习带教中的应用探讨,旨在充分运用MALDI-TOF MS技术结合传统鉴定技术,提高临床微生物检验实习的教学能力。
文摘CHCA as a matrix facilitates the ionization of proteins and peptides in a matrix-assisted laser desorption/ionization(MALDI) time-of-flight(TOF) mass spectrometer. The matrix itself also ionizes and so do its adducts. Matrix clusters and adducts interfere with peptide ionization and peptide mass spectrum interpretation, especially at low sample concentrations and within m/z 500-1 000 range. These matrix adducts, especially within m/z 500-1 000, are significantly reduced with addition of ammonium citrate, ammonium sulfate, ammonium benzoate, ammonium bicarbonate, ammonium citrate and potassium dihydrogen phosphate to the matrix and the samples were deposited onto the MALDI target. The reduction of matrix adducts improves peptide ionization and signal-to-noise ratio, which enhances the reliability of database searching.
