Heparin-induced thrombocytopenia(HIT) is a relatively infrequent complication of heparin administration. HIT can cause devastating thrombosis, making it one of the most serious adverse drug reactions encountered in cl...Heparin-induced thrombocytopenia(HIT) is a relatively infrequent complication of heparin administration. HIT can cause devastating thrombosis, making it one of the most serious adverse drug reactions encountered in clinical practice. We successfully treated a case of severe HIT presenting with thrombosis and life-threatening bleeding complications with intravenous immunoglobulin(IVIG), platelet transfusion and oral anticoagulant Rivaroxaban. In this case, we considered that IVIG played the most important role by preventing further thrombosis, increasing the platelet count, and ensuring the efficacy of Rivaroxaban. We therefore suggest that IVIG might be the optimal treatment for patients with this urgent condition.展开更多
Application of polymerase chain reaction (PCR) to the hypervariable segment of the immunoglobulin heavy chain (IgH) gene allows detection of minimal residual disease (MRD) at a level of one leukemic cell in 103 104 no...Application of polymerase chain reaction (PCR) to the hypervariable segment of the immunoglobulin heavy chain (IgH) gene allows detection of minimal residual disease (MRD) at a level of one leukemic cell in 103 104 normal marrow cells. We used seminested PCR to amplify the DNA fragment of the complementarity-determining region-Ⅲ of the IgH gene from leukemic cell specimens of patients with leukemia. There was IgH gene rearrangement in 25 of 34 ( 74% ) acute lymphohlastic leukemia (ALL) patients, 3 of 4 (75%) chronic lymphocytic leukemia (CLL) patients. Five of 33 (15%) acute myeloid leukemia (AML) patients and 0 of 9 (0%) chronic myeloid leukemia (CML) patients. ALL PCR positive cases were confirmed by Southern blot analysis. Our results indicated that (1) the seminested PCR technique was found to have a higher sensitivity and less false-negative results than onestage PCR; (2) IgH gene rearrangement may not be Iimited to lymphoid leukemia of B cell lineage. In some patients,the leukemic transforming event may involve stem cells capable of both B cell and myeloid differentiation, or AML cells may differentiate along different lineages with the predominant appearance of one or the other subclone in the course of the disease. The mechanism needs to be further investigated.展开更多
An immunocytochemical study was performed in 6 peripheral nerve specimens from 6 cases of polymyositis. The results revealed that depositions of IgG, IgM, IgA and C3 were found in the epineurium, perineurium and the w...An immunocytochemical study was performed in 6 peripheral nerve specimens from 6 cases of polymyositis. The results revealed that depositions of IgG, IgM, IgA and C3 were found in the epineurium, perineurium and the walls of capillaries. These findings demonstrated that depositions of immunoglobulins and the complement-mediated immunoreaction may play an important role in pathogenesis of polymyositis with peripheral nerve involvements.展开更多
文摘Heparin-induced thrombocytopenia(HIT) is a relatively infrequent complication of heparin administration. HIT can cause devastating thrombosis, making it one of the most serious adverse drug reactions encountered in clinical practice. We successfully treated a case of severe HIT presenting with thrombosis and life-threatening bleeding complications with intravenous immunoglobulin(IVIG), platelet transfusion and oral anticoagulant Rivaroxaban. In this case, we considered that IVIG played the most important role by preventing further thrombosis, increasing the platelet count, and ensuring the efficacy of Rivaroxaban. We therefore suggest that IVIG might be the optimal treatment for patients with this urgent condition.
文摘Application of polymerase chain reaction (PCR) to the hypervariable segment of the immunoglobulin heavy chain (IgH) gene allows detection of minimal residual disease (MRD) at a level of one leukemic cell in 103 104 normal marrow cells. We used seminested PCR to amplify the DNA fragment of the complementarity-determining region-Ⅲ of the IgH gene from leukemic cell specimens of patients with leukemia. There was IgH gene rearrangement in 25 of 34 ( 74% ) acute lymphohlastic leukemia (ALL) patients, 3 of 4 (75%) chronic lymphocytic leukemia (CLL) patients. Five of 33 (15%) acute myeloid leukemia (AML) patients and 0 of 9 (0%) chronic myeloid leukemia (CML) patients. ALL PCR positive cases were confirmed by Southern blot analysis. Our results indicated that (1) the seminested PCR technique was found to have a higher sensitivity and less false-negative results than onestage PCR; (2) IgH gene rearrangement may not be Iimited to lymphoid leukemia of B cell lineage. In some patients,the leukemic transforming event may involve stem cells capable of both B cell and myeloid differentiation, or AML cells may differentiate along different lineages with the predominant appearance of one or the other subclone in the course of the disease. The mechanism needs to be further investigated.
文摘An immunocytochemical study was performed in 6 peripheral nerve specimens from 6 cases of polymyositis. The results revealed that depositions of IgG, IgM, IgA and C3 were found in the epineurium, perineurium and the walls of capillaries. These findings demonstrated that depositions of immunoglobulins and the complement-mediated immunoreaction may play an important role in pathogenesis of polymyositis with peripheral nerve involvements.
