Drug delivery systems(DDS)have recently emerged as a promising approach for the unique advantages of drug protection and targeted delivery.However,the access of nanoparticles/drugs to the central nervous system(CNS)re...Drug delivery systems(DDS)have recently emerged as a promising approach for the unique advantages of drug protection and targeted delivery.However,the access of nanoparticles/drugs to the central nervous system(CNS)remains a challenge mainly due to the obstruction from brain barriers.Immune cells infiltrating the CNS in the pathological state have inspired the development of strategies for CNS foundation drug delivery.Herein,we outline the three major brain barriers in the CNS and the mechanisms by which immune cells migrate across the blood–brain barrier.We subsequently review biomimetic strategies utilizing immune cell-based nanoparticles for the delivery of nanoparticles/drugs to the CNS,as well as recent progress in rationally engineering immune cell-based DDS for CNS diseases.Finally,we discuss the challenges and opportunities of immune cell-based DDS in CNS diseases to promote their clinical development.展开更多
In this paper,the security problem for the multi-access edge computing(MEC)network is researched,and an intelligent immunity-based security defense system is proposed to identify the unauthorized mobile users and to p...In this paper,the security problem for the multi-access edge computing(MEC)network is researched,and an intelligent immunity-based security defense system is proposed to identify the unauthorized mobile users and to protect the security of whole system.In the proposed security defense system,the security is protected by the intelligent immunity through three functions,identification function,learning function,and regulation function,respectively.Meanwhile,a three process-based intelligent algorithm is proposed for the intelligent immunity system.Numerical simulations are given to prove the effeteness of the proposed approach.展开更多
Current worm detection methods are unable to detect multi-vector polymorphic worms effectively. Based on negative selection mechanism of the immune system, a local network worm detection system that detects worms was ...Current worm detection methods are unable to detect multi-vector polymorphic worms effectively. Based on negative selection mechanism of the immune system, a local network worm detection system that detects worms was proposed. Normal network service requests were represented by self-strings, and the detection system used self-strings to monitor the network for anomaly. According to the properties of worm propagation, a control center correlated the anomalies detected in the form of binary trees to ensure the accuracy of worm detection. Experiments show the system to be effective in detecting the traditional as well as multi-vector polymorphic worms.展开更多
Photothermal and photodynamic therapies(PTT/PDT)hold promise for localized tumor treatment,yet their full potential is hampered by limitations such as the hypoxic tumor microenvironment and inadequate systemic immune ...Photothermal and photodynamic therapies(PTT/PDT)hold promise for localized tumor treatment,yet their full potential is hampered by limitations such as the hypoxic tumor microenvironment and inadequate systemic immune activation.Addressing these challenges,we present a novel near-infrared(NIR)-triggered RNS nanoreactor(PBNO-Ce6)to amplify the photodynamic and photothermal therapy efficacy against triple-negative breast cancer(TNBC).The designed PBNOCe6 combines sodium nitroprusside-doped Prussian Blue nanoparticles with Chlorin e6 to enable on-site RNS production through NIR-induced concurrent NO release and ROS generation.This not only enhances tumor cell eradication but also potentiates local and systemic antitumor immune responses,protecting mice from tumor rechallenge.Our in vivo evaluations revealed that treatment with PBNO-Ce6 leads to a remarkable 2.7-fold increase in cytotoxic T lymphocytes and a 62%decrease in regulatory T cells in comparison to the control PB-Ce6(Prussian Blue nanoparticles loaded with Chlorin e6),marking a substantial improvement over traditional PTT/PDT.As such,the PBNO-Ce6 nanoreactor represents a transformative approach for improving outcomes in TNBC and potentially other malignancies affected by similar barriers.展开更多
Objective Venous thromboembolism is a highly prevalent condition after polytrauma,and recognized as an important factor contributing to poor prognosis.The aim of this study was to investigate the risk factors for lowe...Objective Venous thromboembolism is a highly prevalent condition after polytrauma,and recognized as an important factor contributing to poor prognosis.The aim of this study was to investigate the risk factors for lower extremity deep venous thrombosis(LEDVT)in a severely traumatized population and to evaluate their predictive value for LEDVT.Methods This was a retrospective,single-center observational study.All subjects were severely traumatized patients who were admitted to the Traumatic Intensive Care Unit from January 2021 to May 2024.Based on Doppler ultrasound findings of both lower extremities from the time of injury to 30 days post-injury,patients who developed LEDVT were enrolled in the LEDVT group,and those who did not develop LEDVT were enrolled in the NLEDVT group.Demographic,clinical,and laboratory data were collected upon admission.Multivariable logistic regression analysis was performed to identify risk factors for LEDVT.Receiver operating characteristic(ROC)curve was used to evaluate the overall fit of the final model.Results There were 56 patients enrolled in the LEDVT group and 81 patients in the NLEDVT group.Age,Aggregate Index of Systemic Inflammation(AISI),Systemic Inflammation Response Index(SIRI),ICU length of stay,and albumin were identified as independent risk factors for LEDVT(all P<0.05).The area under their ROC curves were 0.604,0.657,0.694,0.668,and 0.405,respectively.Combined model for early clinical prediction of LEDVT in severely traumatized patients by age,SIRI,AISI,and albumin resulted in an area under the ROC curve of 0.805(95%CI:0.73-0.88,SE=0.037).Conclusion The combination of age,SIRI,AISI,and albumin has a predictive value for LEDVT in severely traumatized patients.展开更多
Vaccinations are essential for preventing and treating disease,especially cancer nanovaccines,which have gained considerable interest recently for their strong anti-tumor immune capabilities.Vaccines can prompt the im...Vaccinations are essential for preventing and treating disease,especially cancer nanovaccines,which have gained considerable interest recently for their strong anti-tumor immune capabilities.Vaccines can prompt the immune system to generate antibodies and activate various immune cells,leading to a response against tumor tissues and reducing the negative effects and recurrence risks of traditional chemotherapy and surgery.To enhance the flexibility and targeting of vaccines,nanovaccines utilize nanotechnology to encapsulate or carry antigens at the nanoscale level,enabling more controlled and precise drug delivery to enhance immune responses.Cancer nanovaccines function by encapsulating tumor-specific antigens or tumor-associated antigens within nanomaterials.The small size of these nanomaterials allows for precise targeting of T cells,dendritic cells,or cancer cells,thereby eliciting a more potent anti-tumor response.In this paper,we focus on the classification of carriers for cancer nanovaccines,the roles of different target cells,and clinically tested cancer nanovaccines,discussing strategies for effectively inducing cytotoxic T lymphocytes responses and optimizing antigen presentation,while also looking ahead to the translational challenges of moving from animal experiments to clinical trials.展开更多
Over one-third of 700,000 military personnel who served in the first Gulf War(GW)suffer from an assortment of symptoms,including cognitive and memory problems,musculoskeletal pain,gastrointestinal discomfort,fatigue,a...Over one-third of 700,000 military personnel who served in the first Gulf War(GW)suffer from an assortment of symptoms,including cognitive and memory problems,musculoskeletal pain,gastrointestinal discomfort,fatigue,and respiratory issues[1,2].The precise etiology of Gulf War illness(GWI)is unclear.However,epidemiological and preclinical studies imply that exposures to the prophylactic drug pyridostigmine bromide,insecticides,pesticides,smoke from oil well fires,and interaction between these exposures and war-related stress underlie this illness[2].As per the Kansas case definition,GWI is a chronic multi-symptom illness displaying one moderately severe and/or multiple symptoms of any severity in at least 3 of 6 symptom domains(fatigue,pain,neurological/cognitive/mood,skin,gastrointestinal,respiratory)[1,2].While the exact pathophysiological changes underlying GWI have not been identified,alterations in immune regulation and dysregulation of the redox balance have been observed in GWI,resulting in chronic systemic inflammation and neuroinflammation[2].展开更多
The gut microbiota, the largest symbiotic ecosystem with the host, has been shown to play important roles in maintaining intestinal homeostasis. Dysbiosis of the gut microbiome is caused by the imbalance between the c...The gut microbiota, the largest symbiotic ecosystem with the host, has been shown to play important roles in maintaining intestinal homeostasis. Dysbiosis of the gut microbiome is caused by the imbalance between the commensal and pathogenic microbiomes. The commensal microbiome regulates the maturation of the mucosal immune system, while the pathogenic microbiome causes immunity dysfunction, resulting in disease development.The gut mucosal immune system, which consists of lymph nodes, lamina propria and epithelial cells, constitutes a protective barrier for the integrity of the intestinal tract. The composition of the gut microbiota is under the surveillance of the normal mucosal immune system. Inflammation, which is caused by abnormal immune responses,influences the balance of the gut microbiome, resulting in intestinal diseases. In this review, we briefly outlined the interaction between the gut microbiota and the immune system and provided a reference for future studies.展开更多
In order to eliminate false alarms,issued by gas sensors in coal mining,caused by Electromagnetic Interference(EMI),both computer simulation and field measurements were introduced to analyze the underground EMI distri...In order to eliminate false alarms,issued by gas sensors in coal mining,caused by Electromagnetic Interference(EMI),both computer simulation and field measurements were introduced to analyze the underground EMI distribution.A simplified model of a sensor with metal enclosure was established and the effects of shielding properties about the enclosure aperture were studied.Because the haulage motor is the moving EMI source,varying with time,the onsite flameproof measuring instruments cannot accomplish synchronous measurements of electromagnetic field vectors.To simplify the field measurements,two sensors,one with a lead and the other without a lead,were chosen to conduct the contrasting measurements.The EMI current caused by the perforation lead was comparatively strong and therefore nickel zinc ferrite beads were used to cut off the EMI propagation paths.The peak value of the interference current was reduced by 20%-70% with the beads.After switching on the sensor power,the sen-sors still occasionally gave false alarms when the switch of nearby large-scale electric equipment was operated.A complex EMI filter was used and the EMI attenuated markedly.The running results demonstrated that false alarms had been eliminated.We con-clude that the improved shielding and filtering are highly significant in enhancing the immunity of the gas sensor.展开更多
A monitoring system is an important guarantee of safety in a production mine. However, not all monitoring substations pass the electrical fast transient/burst （EFT/B） immunity test and the explosion-proof test simul...A monitoring system is an important guarantee of safety in a production mine. However, not all monitoring substations pass the electrical fast transient/burst （EFT/B） immunity test and the explosion-proof test simultaneously. To enhance the immunity, the values of capacitance and inductance should be increased, which are actually limited by coal mine explosion-proof standards. Hence, for the first time, an active filter was applied in an electromagnetic interference （EMI） output filter. As a result, the interference within 30 MHz clearly weakened, but the frequency spectrum had a wide range. An EMI input filter and ferrite beads were adopted to restrain higher frequency interference. An output interference spectrogram of the substation was obtained with an analyzer. The results indicate that the improved complex filtering markedly help to control interference. With the support of improved complex filtering and other enhancing immunity means about I/O ports, the substation managed to pass both the EFT/B immunity test and the explosion-proof test synchronously. We conclude that improved complex filtering is of vital importance in enhancing the electromagnetic compatibilitv （EMC） of the coal mine monitoring system.展开更多
Obesity-induced insulin resistance is the hallmark of metabolic syndrome,and chronic,low-grade tissue inflammation links obesity to insulin resistance through the activation of tissue-infiltrating immune cells.Current...Obesity-induced insulin resistance is the hallmark of metabolic syndrome,and chronic,low-grade tissue inflammation links obesity to insulin resistance through the activation of tissue-infiltrating immune cells.Current therapeutic approaches lack efficacy and immunomodulatory capacity.Thus,a new therapeutic approach is needed to prevent chronic inflammation and alleviate insulin resistance.Here,we synthesized a tetrahedral framework nucleic acid(tFNA)nanoparticle that carried resveratrol(RSV)to inhibit tissue inflammation and improve insulin sensitivity in obese mice.The prepared nanoparticles,namely tFNAs-RSV,possessed the characteristics of simple synthesis,stable properties,good water solubility,and superior biocompatibility.The tFNA-based delivery ameliorated the lability of RSV and enhanced its therapeutic efficacy.In high-fat diet(HFD)-fed mice,the administration of tFNAs-RSV ameliorated insulin resistance by alleviating inflammation status.tFNAs-RSV could reverse M1 phenotype macrophages in tissues to M2 phenotype macrophages.As for adaptive immunity,the prepared nanoparticles could repress the activation of Th1 and Th17 and promote Th2 and Treg,leading to the alleviation of insulin resistance.Furthermore,this study is the first to demonstrate that tFNAs,a nucleic acid material,possess immunomodulatory capacity.Collectively,our findings demonstrate that tFNAs-RSV alleviate insulin resistance and ameliorate inflammation in HFD mice,suggesting that nucleic acid materials or nucleic acid-based delivery systems may be a potential agent for the treatment of insulin resistance and obesity-related metabolic diseases.展开更多
Sepsis and related syndromes are the major cause of multiple organ failure and death in patients with critical illnesses. Neuroendocrine dysfunction has long been thought to be an important event in sepsis. In clinic,...Sepsis and related syndromes are the major cause of multiple organ failure and death in patients with critical illnesses. Neuroendocrine dysfunction has long been thought to be an important event in sepsis. In clinic, optimal management of the hormones could alleviate severe complications in sepsis. In this article, we review the dysfunction ofneuroendocrine system as well as autonomic nervous system in sepsis, and summarize the respective therapy strategies.展开更多
BACKGROUND:Periplaneta americana extract is recognized to have a positive effect on gastrointestinal mucosa.This study aimed to investigate the effects of periplaneta americana extract on immune function,nutrition sta...BACKGROUND:Periplaneta americana extract is recognized to have a positive effect on gastrointestinal mucosa.This study aimed to investigate the effects of periplaneta americana extract on immune function,nutrition status and gastrointestinal complications of early enteral nutrition patients with systemic inflammatory response syndrome(SIRS).METHODS:Patients with SIRS were randomly divided into two groups:treatment and control groups.All patients in the two groups received conventional therapy including enteral nutrition,but periplaneta americana extract,an additional Chinese medicine,was given to the patients in the treatment group.At the beginning of treatment(0 day)and 1,3,and 7 days after treatment,the levels of immunoglobulin(Ig A),total lymphocyte count(TLC),total protein(TP)and prealbumin(PA)were respectively tested in patients'venous blood.The incidences of bloating,diarrhea,aspiration pneumonia and high blood sugar at 7 days after treatment were recorded.The mortality of the patients in 28 days was recorded.RESULTS:At 3 and 7 days after treatment,the levels of Ig A and TLC in the treatment group were higher than those in the control group(P<0.05).At 7 days after treatment,the levels of TP and PA in the treatment group were higher than those in the control group(P<0.05).The incidences of bloating and diarrhea in the treatment group were lower than those in the control group,the differences were significant(P<0.05).The mortality of treatment group was lower than that of the control group(P>0.05).CONCLUSION:Periplaneta americana extract could reduce gastrointestinal complications and improve immune function and nutritional status in patients with systemic inflammatory response syndrome.展开更多
This paper focuses on investigating immunological principles in designing a multi-agent security architecture for intrusion detection and response in wireless mesh networks.In this approach,the immunity-based agents m...This paper focuses on investigating immunological principles in designing a multi-agent security architecture for intrusion detection and response in wireless mesh networks.In this approach,the immunity-based agents monitor the situation in the network.These agents can take appropriate actions according to the underlying security policies.Specifically,their activities are coordinated in a hierarchical fashion while sensing,communicating,determining and generating responses.Such an agent can learn about and adapt to its environment dynamically and can detect both known and unknown intrusions.The proposed intrusion detection architecture is designed to be flexible,extendible,and adaptable so that it can perform real-time monitoring.This paper provides the conceptual view and a general framework of the proposed system.In the end,the architecture is illustrated by an example and by simulation to show it can prevent attacks efficiently.展开更多
A clone selection algorithm for computer immune system is presented. Clone selection principles in biological immune system are applied to the domain of computer virus detection. Based on the negative selection algori...A clone selection algorithm for computer immune system is presented. Clone selection principles in biological immune system are applied to the domain of computer virus detection. Based on the negative selection algorithm proposed by Stephanie Forrest, combining mutation operator in genetic algorithms and niching strategy in biology is adopted, the number of detectors is decreased effectively and the ability on self-nonself discrimination is improved. Simulation experiment shows that the algorithm is simple, practical and is adapted to the discrimination for long files.展开更多
Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet.These valuable organisms are more than food,they fit in perfectly as a novel market group known as nutra-mycoceuticals.Immune-b...Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet.These valuable organisms are more than food,they fit in perfectly as a novel market group known as nutra-mycoceuticals.Immune-balancing mushroom dietary fibers and secondary metabolites such as polyphenols are the main focus of the healthcare industry.Wellness and cosmetic companies are increasingly using mushroom extracts rich in these ingredients.This review considers the basic molecular immunomodulatory mechanisms of action of the most commonly used mushroom dietary fibers,β-glucans.The literature data on their bioavailability,metabolic transformations,preclinical and human clinical research,and safety are discussed.Immunomodulatory mechanisms of polyphenol ingredients are also considered.These molecules present great potential in the design of the new immunity balancer formulations according to their widespread structural diversity.Finally,we draw attention to the perspectives of modern trends in mushroom nutraceutical and cosmeceutical formulations to strengthen and balance immunity.展开更多
In order to solve the problem that me traditional signature-based detection technology cannot effectively detect unknown malware, we propose in this study a smartphone malware detection model （SP-MDM） based on artif...In order to solve the problem that me traditional signature-based detection technology cannot effectively detect unknown malware, we propose in this study a smartphone malware detection model （SP-MDM） based on artificial immune system, in which static malware analysis and dynamic malware analysis techniques are combined, and antigens are generated by encoding the characteristics extracted from the malware. Based on negative selection algorithm, the mature detectors are generated. By introducing clonal selection algorithm, the detectors with higher affinity are selected to undergo a proliferation and somatic hyper-mutation process, so that more excellent detector offspring can be generated. Experimental result shows that the detection model has a higher detection rate for unknown smartphone malware, and better detection performance can be achieved by increasing the clone generation.展开更多
The effects of dietary zinc on the immune organs and cellular immunity were investigated in the rats of zinc-deficient(ZD), zinc-excessive(ZE), paired feeding(PF) and ad libitus(AL) groups. The findings were as follow...The effects of dietary zinc on the immune organs and cellular immunity were investigated in the rats of zinc-deficient(ZD), zinc-excessive(ZE), paired feeding(PF) and ad libitus(AL) groups. The findings were as follows: (1) The weight of the thymus and spleen and the ratio with the body weight, the size of the cells,the production of thymic peptides and thymin activity were significantly lower in ZD and ZE groups than in PF and AL groups. (2) The total number of the cells in G_0/G_1 phase of T-and B-cells and bone marrow cells were significantly higher in ZD and ZF groups than in PF and AL groups while that of the cells in S and G_2/M phases were significantly lower in ZD and ZE groups than in PF and AL groups. (3) The cellular DNA, RNA and protein content in the thymus, spleen and bone marrow were significantly lower in ZD and ZE groups than in PF and AL groups.(4) The ￣3H-TdR incorporation rate of splenic T-and B-cells , IL-2 activity, plasma level of TNF and nitrogen oxide(NO) were significantly lower in ZD group than in PF group. (5) The leucocyte count of the perpheral blood,the percentage of lymphocytes, the bacteriocidal activity of phagocytes, and the phagocytic rate and index of abdominal phagocytes were significantly lower in ZD and ZE groups than in PF and AL groups. These findings indicate that an adequate amount of dietary zinc is essential for the development of immune organs and the promotion of cellular immunity while deficiency or excess of zinc is able to damage the immune function.展开更多
This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici str...This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici strains with antibacterial activity(P.acidilactici CCFM28 and CCFM18)were obtained based on the inhibition-zone assay.The produced components were identified as bacteriocins through protease treatment,pH adjustment and hydrogen peroxide treatment.Bacteriocin-producing and non-bacteriocin-producing P.acidilactici strains(P.acidilactici CCFM28,CCFM18 and NT17-3)caused significant changes in serum immune factors and intestinal flora of normal mice.After 14 days of intervention,the relative abundance of Firmicutes was significantly decreased,but that of Proteobacteria was significantly increased at the phylum level.At the genus level,the administration of three P.acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus.Furthermore,there were also different regulations on some probiotic strains,such as Bifidobacterium,Coprococcus and Akkermansia,which were closely related to the antibacterial ability of the bacteriocin and the type of strain.The results indicated that the intervention of different P.acidilactici strains could differently change the structure of intestinal flora in normal mice,which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.展开更多
We investigate the stochastic responses of a tumor–immune system competition model with environmental noise and periodic treatment. Firstly, a mathematical model describing the interaction between tumor cells and imm...We investigate the stochastic responses of a tumor–immune system competition model with environmental noise and periodic treatment. Firstly, a mathematical model describing the interaction between tumor cells and immune system under external fluctuations and periodic treatment is established based on the stochastic differential equation. Then, sufficient conditions for extinction and persistence of the tumor cells are derived by constructing Lyapunov functions and Ito＇s formula. Finally, numerical simulations are introduced to illustrate and verify the results. The results of this work provide the theoretical basis for designing more effective and precise therapeutic strategies to eliminate cancer cells, especially for combining the immunotherapy and the traditional tools.展开更多
基金supported by the National Natural Science Foundation of China(82204634,82174047,81622051)the Zhejiang Provincial Natural Science Foundation of China(LQ22H280010)the Foundation of Zhejiang Chinese Medical University(2021ZR03).
文摘Drug delivery systems(DDS)have recently emerged as a promising approach for the unique advantages of drug protection and targeted delivery.However,the access of nanoparticles/drugs to the central nervous system(CNS)remains a challenge mainly due to the obstruction from brain barriers.Immune cells infiltrating the CNS in the pathological state have inspired the development of strategies for CNS foundation drug delivery.Herein,we outline the three major brain barriers in the CNS and the mechanisms by which immune cells migrate across the blood–brain barrier.We subsequently review biomimetic strategies utilizing immune cell-based nanoparticles for the delivery of nanoparticles/drugs to the CNS,as well as recent progress in rationally engineering immune cell-based DDS for CNS diseases.Finally,we discuss the challenges and opportunities of immune cell-based DDS in CNS diseases to promote their clinical development.
基金This work was supported by National Natural Science Foundation of China(No.61971026)the Fundamental Research Funds for the Central Universities(No.FRF-TP-18-008A3).
文摘In this paper,the security problem for the multi-access edge computing(MEC)network is researched,and an intelligent immunity-based security defense system is proposed to identify the unauthorized mobile users and to protect the security of whole system.In the proposed security defense system,the security is protected by the intelligent immunity through three functions,identification function,learning function,and regulation function,respectively.Meanwhile,a three process-based intelligent algorithm is proposed for the intelligent immunity system.Numerical simulations are given to prove the effeteness of the proposed approach.
文摘Current worm detection methods are unable to detect multi-vector polymorphic worms effectively. Based on negative selection mechanism of the immune system, a local network worm detection system that detects worms was proposed. Normal network service requests were represented by self-strings, and the detection system used self-strings to monitor the network for anomaly. According to the properties of worm propagation, a control center correlated the anomalies detected in the form of binary trees to ensure the accuracy of worm detection. Experiments show the system to be effective in detecting the traditional as well as multi-vector polymorphic worms.
基金the financial support from the National Natural Science Foundation of China (No. 82372019, 82022034, 82173327)Jiangsu Province Natural Science Foundation of China (BK20200032)Double First Class Foundation of China Pharmaceutical University(CPUQNJC22_03)
文摘Photothermal and photodynamic therapies(PTT/PDT)hold promise for localized tumor treatment,yet their full potential is hampered by limitations such as the hypoxic tumor microenvironment and inadequate systemic immune activation.Addressing these challenges,we present a novel near-infrared(NIR)-triggered RNS nanoreactor(PBNO-Ce6)to amplify the photodynamic and photothermal therapy efficacy against triple-negative breast cancer(TNBC).The designed PBNOCe6 combines sodium nitroprusside-doped Prussian Blue nanoparticles with Chlorin e6 to enable on-site RNS production through NIR-induced concurrent NO release and ROS generation.This not only enhances tumor cell eradication but also potentiates local and systemic antitumor immune responses,protecting mice from tumor rechallenge.Our in vivo evaluations revealed that treatment with PBNO-Ce6 leads to a remarkable 2.7-fold increase in cytotoxic T lymphocytes and a 62%decrease in regulatory T cells in comparison to the control PB-Ce6(Prussian Blue nanoparticles loaded with Chlorin e6),marking a substantial improvement over traditional PTT/PDT.As such,the PBNO-Ce6 nanoreactor represents a transformative approach for improving outcomes in TNBC and potentially other malignancies affected by similar barriers.
基金Basic Research Spe-cial Project of Suzhou Science and Technology Bureau(SSD2024050).
文摘Objective Venous thromboembolism is a highly prevalent condition after polytrauma,and recognized as an important factor contributing to poor prognosis.The aim of this study was to investigate the risk factors for lower extremity deep venous thrombosis(LEDVT)in a severely traumatized population and to evaluate their predictive value for LEDVT.Methods This was a retrospective,single-center observational study.All subjects were severely traumatized patients who were admitted to the Traumatic Intensive Care Unit from January 2021 to May 2024.Based on Doppler ultrasound findings of both lower extremities from the time of injury to 30 days post-injury,patients who developed LEDVT were enrolled in the LEDVT group,and those who did not develop LEDVT were enrolled in the NLEDVT group.Demographic,clinical,and laboratory data were collected upon admission.Multivariable logistic regression analysis was performed to identify risk factors for LEDVT.Receiver operating characteristic(ROC)curve was used to evaluate the overall fit of the final model.Results There were 56 patients enrolled in the LEDVT group and 81 patients in the NLEDVT group.Age,Aggregate Index of Systemic Inflammation(AISI),Systemic Inflammation Response Index(SIRI),ICU length of stay,and albumin were identified as independent risk factors for LEDVT(all P<0.05).The area under their ROC curves were 0.604,0.657,0.694,0.668,and 0.405,respectively.Combined model for early clinical prediction of LEDVT in severely traumatized patients by age,SIRI,AISI,and albumin resulted in an area under the ROC curve of 0.805(95%CI:0.73-0.88,SE=0.037).Conclusion The combination of age,SIRI,AISI,and albumin has a predictive value for LEDVT in severely traumatized patients.
基金financially supported by Excellent Young Science Fund for National Natural Science Foundation of China(82022033)Sichuan Science and Technology Program(2024NSFJQ0048)+3 种基金National Natural Science Foundation of China(81902422)Jiangsu Natural Science Foundation(No.BK20231245)Program of Jiangsu Commission of Health(No.M2020024)Program of Yangzhou Commission of Health(No.2023-2-01,2024-2-08).
文摘Vaccinations are essential for preventing and treating disease,especially cancer nanovaccines,which have gained considerable interest recently for their strong anti-tumor immune capabilities.Vaccines can prompt the immune system to generate antibodies and activate various immune cells,leading to a response against tumor tissues and reducing the negative effects and recurrence risks of traditional chemotherapy and surgery.To enhance the flexibility and targeting of vaccines,nanovaccines utilize nanotechnology to encapsulate or carry antigens at the nanoscale level,enabling more controlled and precise drug delivery to enhance immune responses.Cancer nanovaccines function by encapsulating tumor-specific antigens or tumor-associated antigens within nanomaterials.The small size of these nanomaterials allows for precise targeting of T cells,dendritic cells,or cancer cells,thereby eliciting a more potent anti-tumor response.In this paper,we focus on the classification of carriers for cancer nanovaccines,the roles of different target cells,and clinically tested cancer nanovaccines,discussing strategies for effectively inducing cytotoxic T lymphocytes responses and optimizing antigen presentation,while also looking ahead to the translational challenges of moving from animal experiments to clinical trials.
基金Department of Defense(W81XWH-17-1-0447 and W81XWH1910548 to AKS)Texas A&M University School of Medicine。
文摘Over one-third of 700,000 military personnel who served in the first Gulf War(GW)suffer from an assortment of symptoms,including cognitive and memory problems,musculoskeletal pain,gastrointestinal discomfort,fatigue,and respiratory issues[1,2].The precise etiology of Gulf War illness(GWI)is unclear.However,epidemiological and preclinical studies imply that exposures to the prophylactic drug pyridostigmine bromide,insecticides,pesticides,smoke from oil well fires,and interaction between these exposures and war-related stress underlie this illness[2].As per the Kansas case definition,GWI is a chronic multi-symptom illness displaying one moderately severe and/or multiple symptoms of any severity in at least 3 of 6 symptom domains(fatigue,pain,neurological/cognitive/mood,skin,gastrointestinal,respiratory)[1,2].While the exact pathophysiological changes underlying GWI have not been identified,alterations in immune regulation and dysregulation of the redox balance have been observed in GWI,resulting in chronic systemic inflammation and neuroinflammation[2].
基金"PUMC"Fellow award from Peking Union Medical Collage(PUMC)CAMS Initiative for Innovative Medicine(2016-I2M-1-006)
文摘The gut microbiota, the largest symbiotic ecosystem with the host, has been shown to play important roles in maintaining intestinal homeostasis. Dysbiosis of the gut microbiome is caused by the imbalance between the commensal and pathogenic microbiomes. The commensal microbiome regulates the maturation of the mucosal immune system, while the pathogenic microbiome causes immunity dysfunction, resulting in disease development.The gut mucosal immune system, which consists of lymph nodes, lamina propria and epithelial cells, constitutes a protective barrier for the integrity of the intestinal tract. The composition of the gut microbiota is under the surveillance of the normal mucosal immune system. Inflammation, which is caused by abnormal immune responses,influences the balance of the gut microbiome, resulting in intestinal diseases. In this review, we briefly outlined the interaction between the gut microbiota and the immune system and provided a reference for future studies.
基金Project 50674093 supported by the National Natural Science Foundation of China
文摘In order to eliminate false alarms,issued by gas sensors in coal mining,caused by Electromagnetic Interference(EMI),both computer simulation and field measurements were introduced to analyze the underground EMI distribution.A simplified model of a sensor with metal enclosure was established and the effects of shielding properties about the enclosure aperture were studied.Because the haulage motor is the moving EMI source,varying with time,the onsite flameproof measuring instruments cannot accomplish synchronous measurements of electromagnetic field vectors.To simplify the field measurements,two sensors,one with a lead and the other without a lead,were chosen to conduct the contrasting measurements.The EMI current caused by the perforation lead was comparatively strong and therefore nickel zinc ferrite beads were used to cut off the EMI propagation paths.The peak value of the interference current was reduced by 20%-70% with the beads.After switching on the sensor power,the sen-sors still occasionally gave false alarms when the switch of nearby large-scale electric equipment was operated.A complex EMI filter was used and the EMI attenuated markedly.The running results demonstrated that false alarms had been eliminated.We con-clude that the improved shielding and filtering are highly significant in enhancing the immunity of the gas sensor.
基金Project 50674093 supported by the National Natural Science Foundation of China
文摘A monitoring system is an important guarantee of safety in a production mine. However, not all monitoring substations pass the electrical fast transient/burst （EFT/B） immunity test and the explosion-proof test simultaneously. To enhance the immunity, the values of capacitance and inductance should be increased, which are actually limited by coal mine explosion-proof standards. Hence, for the first time, an active filter was applied in an electromagnetic interference （EMI） output filter. As a result, the interference within 30 MHz clearly weakened, but the frequency spectrum had a wide range. An EMI input filter and ferrite beads were adopted to restrain higher frequency interference. An output interference spectrogram of the substation was obtained with an analyzer. The results indicate that the improved complex filtering markedly help to control interference. With the support of improved complex filtering and other enhancing immunity means about I/O ports, the substation managed to pass both the EFT/B immunity test and the explosion-proof test synchronously. We conclude that improved complex filtering is of vital importance in enhancing the electromagnetic compatibilitv （EMC） of the coal mine monitoring system.
基金National Key R&D Program of China(2019YFA0110600)National Natural Science Foundation of China(81970916,81671031)the LU JIAXI International team program supported by the K.C.Wong Education Foundation and CAS and the Youth Innovation Promotion Association of CAS(Grant No.2016236).
文摘Obesity-induced insulin resistance is the hallmark of metabolic syndrome,and chronic,low-grade tissue inflammation links obesity to insulin resistance through the activation of tissue-infiltrating immune cells.Current therapeutic approaches lack efficacy and immunomodulatory capacity.Thus,a new therapeutic approach is needed to prevent chronic inflammation and alleviate insulin resistance.Here,we synthesized a tetrahedral framework nucleic acid(tFNA)nanoparticle that carried resveratrol(RSV)to inhibit tissue inflammation and improve insulin sensitivity in obese mice.The prepared nanoparticles,namely tFNAs-RSV,possessed the characteristics of simple synthesis,stable properties,good water solubility,and superior biocompatibility.The tFNA-based delivery ameliorated the lability of RSV and enhanced its therapeutic efficacy.In high-fat diet(HFD)-fed mice,the administration of tFNAs-RSV ameliorated insulin resistance by alleviating inflammation status.tFNAs-RSV could reverse M1 phenotype macrophages in tissues to M2 phenotype macrophages.As for adaptive immunity,the prepared nanoparticles could repress the activation of Th1 and Th17 and promote Th2 and Treg,leading to the alleviation of insulin resistance.Furthermore,this study is the first to demonstrate that tFNAs,a nucleic acid material,possess immunomodulatory capacity.Collectively,our findings demonstrate that tFNAs-RSV alleviate insulin resistance and ameliorate inflammation in HFD mice,suggesting that nucleic acid materials or nucleic acid-based delivery systems may be a potential agent for the treatment of insulin resistance and obesity-related metabolic diseases.
基金This study was supported by grants from the National Basic Research Program of China （No. 2005CB522602） and the National Natural Science Foundation of China （No. 30973120, No. 30672178, No. 30872683）.
文摘Sepsis and related syndromes are the major cause of multiple organ failure and death in patients with critical illnesses. Neuroendocrine dysfunction has long been thought to be an important event in sepsis. In clinic, optimal management of the hormones could alleviate severe complications in sepsis. In this article, we review the dysfunction ofneuroendocrine system as well as autonomic nervous system in sepsis, and summarize the respective therapy strategies.
基金supported by Tangshan City Science and Technology Development Project(No.15130219a)
文摘BACKGROUND:Periplaneta americana extract is recognized to have a positive effect on gastrointestinal mucosa.This study aimed to investigate the effects of periplaneta americana extract on immune function,nutrition status and gastrointestinal complications of early enteral nutrition patients with systemic inflammatory response syndrome(SIRS).METHODS:Patients with SIRS were randomly divided into two groups:treatment and control groups.All patients in the two groups received conventional therapy including enteral nutrition,but periplaneta americana extract,an additional Chinese medicine,was given to the patients in the treatment group.At the beginning of treatment(0 day)and 1,3,and 7 days after treatment,the levels of immunoglobulin(Ig A),total lymphocyte count(TLC),total protein(TP)and prealbumin(PA)were respectively tested in patients'venous blood.The incidences of bloating,diarrhea,aspiration pneumonia and high blood sugar at 7 days after treatment were recorded.The mortality of the patients in 28 days was recorded.RESULTS:At 3 and 7 days after treatment,the levels of Ig A and TLC in the treatment group were higher than those in the control group(P<0.05).At 7 days after treatment,the levels of TP and PA in the treatment group were higher than those in the control group(P<0.05).The incidences of bloating and diarrhea in the treatment group were lower than those in the control group,the differences were significant(P<0.05).The mortality of treatment group was lower than that of the control group(P>0.05).CONCLUSION:Periplaneta americana extract could reduce gastrointestinal complications and improve immune function and nutritional status in patients with systemic inflammatory response syndrome.
基金supported by National Natural Science Foundation of China under Grant No.60932003National High Technical Research and Development Program of China(863 program) Grant No.2007AA01Z452,No.2009AA01Z118+1 种基金Shanghai Municipal Natural Science Foundation under Grant No.09ZR1414900National Undergraduate Innovative Test Program under Grant No.091024812
文摘This paper focuses on investigating immunological principles in designing a multi-agent security architecture for intrusion detection and response in wireless mesh networks.In this approach,the immunity-based agents monitor the situation in the network.These agents can take appropriate actions according to the underlying security policies.Specifically,their activities are coordinated in a hierarchical fashion while sensing,communicating,determining and generating responses.Such an agent can learn about and adapt to its environment dynamically and can detect both known and unknown intrusions.The proposed intrusion detection architecture is designed to be flexible,extendible,and adaptable so that it can perform real-time monitoring.This paper provides the conceptual view and a general framework of the proposed system.In the end,the architecture is illustrated by an example and by simulation to show it can prevent attacks efficiently.
文摘A clone selection algorithm for computer immune system is presented. Clone selection principles in biological immune system are applied to the domain of computer virus detection. Based on the negative selection algorithm proposed by Stephanie Forrest, combining mutation operator in genetic algorithms and niching strategy in biology is adopted, the number of detectors is decreased effectively and the ability on self-nonself discrimination is improved. Simulation experiment shows that the algorithm is simple, practical and is adapted to the discrimination for long files.
基金supported by the Science Fund of the Republic of Serbia,#Grant No:7748088。
文摘Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet.These valuable organisms are more than food,they fit in perfectly as a novel market group known as nutra-mycoceuticals.Immune-balancing mushroom dietary fibers and secondary metabolites such as polyphenols are the main focus of the healthcare industry.Wellness and cosmetic companies are increasingly using mushroom extracts rich in these ingredients.This review considers the basic molecular immunomodulatory mechanisms of action of the most commonly used mushroom dietary fibers,β-glucans.The literature data on their bioavailability,metabolic transformations,preclinical and human clinical research,and safety are discussed.Immunomodulatory mechanisms of polyphenol ingredients are also considered.These molecules present great potential in the design of the new immunity balancer formulations according to their widespread structural diversity.Finally,we draw attention to the perspectives of modern trends in mushroom nutraceutical and cosmeceutical formulations to strengthen and balance immunity.
基金This work was supported in part by National Natural Science Foundation of China under Grants No.61101108，National S&T Major Program under Grants No.2011ZX03002-005-01
文摘In order to solve the problem that me traditional signature-based detection technology cannot effectively detect unknown malware, we propose in this study a smartphone malware detection model （SP-MDM） based on artificial immune system, in which static malware analysis and dynamic malware analysis techniques are combined, and antigens are generated by encoding the characteristics extracted from the malware. Based on negative selection algorithm, the mature detectors are generated. By introducing clonal selection algorithm, the detectors with higher affinity are selected to undergo a proliferation and somatic hyper-mutation process, so that more excellent detector offspring can be generated. Experimental result shows that the detection model has a higher detection rate for unknown smartphone malware, and better detection performance can be achieved by increasing the clone generation.
文摘The effects of dietary zinc on the immune organs and cellular immunity were investigated in the rats of zinc-deficient(ZD), zinc-excessive(ZE), paired feeding(PF) and ad libitus(AL) groups. The findings were as follows: (1) The weight of the thymus and spleen and the ratio with the body weight, the size of the cells,the production of thymic peptides and thymin activity were significantly lower in ZD and ZE groups than in PF and AL groups. (2) The total number of the cells in G_0/G_1 phase of T-and B-cells and bone marrow cells were significantly higher in ZD and ZF groups than in PF and AL groups while that of the cells in S and G_2/M phases were significantly lower in ZD and ZE groups than in PF and AL groups. (3) The cellular DNA, RNA and protein content in the thymus, spleen and bone marrow were significantly lower in ZD and ZE groups than in PF and AL groups.(4) The ￣3H-TdR incorporation rate of splenic T-and B-cells , IL-2 activity, plasma level of TNF and nitrogen oxide(NO) were significantly lower in ZD group than in PF group. (5) The leucocyte count of the perpheral blood,the percentage of lymphocytes, the bacteriocidal activity of phagocytes, and the phagocytic rate and index of abdominal phagocytes were significantly lower in ZD and ZE groups than in PF and AL groups. These findings indicate that an adequate amount of dietary zinc is essential for the development of immune organs and the promotion of cellular immunity while deficiency or excess of zinc is able to damage the immune function.
基金supported by the National Natural Science Foundation of China Program(32021005)the Natural Science Foundation of Jiangsu Province(BK20200084)+2 种基金Projects of Innovation and Development Pillar Program for Key Industries in Southern Xinjiang of Xinjiang Production and Construction Corps(2018DB002)National First Class Discipline Program of Food Science and Technology(JUFSTR20180102)the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice.Two P.acidilactici strains with antibacterial activity(P.acidilactici CCFM28 and CCFM18)were obtained based on the inhibition-zone assay.The produced components were identified as bacteriocins through protease treatment,pH adjustment and hydrogen peroxide treatment.Bacteriocin-producing and non-bacteriocin-producing P.acidilactici strains(P.acidilactici CCFM28,CCFM18 and NT17-3)caused significant changes in serum immune factors and intestinal flora of normal mice.After 14 days of intervention,the relative abundance of Firmicutes was significantly decreased,but that of Proteobacteria was significantly increased at the phylum level.At the genus level,the administration of three P.acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus.Furthermore,there were also different regulations on some probiotic strains,such as Bifidobacterium,Coprococcus and Akkermansia,which were closely related to the antibacterial ability of the bacteriocin and the type of strain.The results indicated that the intervention of different P.acidilactici strains could differently change the structure of intestinal flora in normal mice,which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11402157 and 11571009)Shanxi Scholarship Council of China(Grant No.2015-032)+1 种基金Technological Innovation Programs of Higher Education Institutions in Shanxi,China(Grant No.2015121)Applied Basic Research Programs of Shanxi Province,China(Grant No.2016021013)
文摘We investigate the stochastic responses of a tumor–immune system competition model with environmental noise and periodic treatment. Firstly, a mathematical model describing the interaction between tumor cells and immune system under external fluctuations and periodic treatment is established based on the stochastic differential equation. Then, sufficient conditions for extinction and persistence of the tumor cells are derived by constructing Lyapunov functions and Ito＇s formula. Finally, numerical simulations are introduced to illustrate and verify the results. The results of this work provide the theoretical basis for designing more effective and precise therapeutic strategies to eliminate cancer cells, especially for combining the immunotherapy and the traditional tools.
