Objective Pulmonary arterial hypertension(PAH)is mainly characterized by pulmonary artery obstruction,which is diagnosed by a mean pulmonary artery pressure≥25 mm Hg at rest,and excluding other known causes of pulmon...Objective Pulmonary arterial hypertension(PAH)is mainly characterized by pulmonary artery obstruction,which is diagnosed by a mean pulmonary artery pressure≥25 mm Hg at rest,and excluding other known causes of pulmonary hypertension.To identify genetic mutations and help make a precise diagnosis,we performed genetic testing in 191 probands with invasively confirmed PAH and tried to analyze the genotype-phenotype correlation.展开更多
Background The reversibility of pulmonary arterial hypertension(PAH)in congenital heart disease(CHD)is of great importance for the operability of CHD.Proteomics analysis found that transgelin was significantly upregul...Background The reversibility of pulmonary arterial hypertension(PAH)in congenital heart disease(CHD)is of great importance for the operability of CHD.Proteomics analysis found that transgelin was significantly upregulated in the lung tissue of CHD-PAH patients,especially in the irreversible group.However,how exactly it participated in CHD-PAH development is unknown.展开更多
Background and Objective Pulmonary hypertension (PH) is a fatal disease resulting from various causes. Studies from abroad have shown iron deficiency (ID) is closely associated with disease progression.This associatio...Background and Objective Pulmonary hypertension (PH) is a fatal disease resulting from various causes. Studies from abroad have shown iron deficiency (ID) is closely associated with disease progression.This association is common in pulmonary arterial hypertension (PAH)that belongs to World Health Organization group 1 PH. However, ID prevalence in Chinese patients suffering from PH is unclear so far.展开更多
OBJECTIVE Nowadays,with the living habits and diet structure changes,the morbidity and mortality of hypertension has been rising yearly,which induced by adverse lifestyles,excessive alcohol and poor diet.However,the a...OBJECTIVE Nowadays,with the living habits and diet structure changes,the morbidity and mortality of hypertension has been rising yearly,which induced by adverse lifestyles,excessive alcohol and poor diet.However,the animal models of hypertension resulted from adverse lifestyles have rarely been reported.It is essential to set up some more systematic models to study the characters of hypertension and evaluate the efficacy of traditional Chinese medicine(TCM).METHODS To build the rat model of human lifehabitual hypertension,SD rats had free access to alcohol with high-sucrose-fat diet(AHSFD),alcohol with high-fat diet(AHFD),high-sucrose-fat diet(HSFD),high-fat diet(HFD)and alcohol etc.Various of indicators were detected systematically to explore the features of hypertension models which included blood pressure(BP),liver and kidney functions,the lipid profiles and indexes quantifying TCM symptoms.Then we choose one of the stable and sustained animal models,studying the effects of RPA.E(which extracted from a TCM)on modulating the level of hypertension and the preliminary mechanism in this abstract.RESULTS ①The BP level,serum TC,TG,LDL-C of AHSFD-induced and AHFD-induced rats increased significantly,while the HDL-C reduced in the 4th week.② The BP level HSFD-induced rats increased significantly in the 6th week,serum TC,TG increased in 10 th week.③ The BP level of alcohol-induced rats increased significantly in the 9th week,serum TC,TG increased in 24 th week.④ The level of serum ALT,AST,UA and Cr of all model rats increased significantly after 12 weeks.Meanwhile the microcirculation increased significantly after8 weeks.⑤ After 4-week administration,RPA.E could significantly reduce BP of AHFD-induced hypertensive rats,and could reduce the serum TC,LDL-C,HDL-C,ALT,AST level after 8-week.CONCLUSION The rats induced by AHSFD,AHFD,HSFD and alcohol can raise BP obviously with underlying hepatic and kidney damage,the lipid profiles disorder and TCM symptoms change,while the AH(S)FD-induced hypertension models are earlier and more stable.RPA.E could mildly reduce BP level,and improve the lipid profiles disorders,hepatic damage,which reflects the characteristics of TCM on antihypertensive effects.The above models have their own characteristics,can be used to study the causes and pathogenesis of hypertension complicating metabolic disorder and the related treatment drug screening.展开更多
Objective Studies of the association of hypertension with long-term exposure to particulate matter less than 2.5μm in diameter(PM2.5)have shown contradictory results and the magnitude of the association is still uncl...Objective Studies of the association of hypertension with long-term exposure to particulate matter less than 2.5μm in diameter(PM2.5)have shown contradictory results and the magnitude of the association is still unclear.Hence,we conducted a meta-analysis of cohort studies to assess the effect of long-term exposure to PM2.5 on incident hypertension.展开更多
Aim The aim of this investigation was to evaluate gender differences in development of renovascular hy- pertension. Methods The experiments were performed with 10-week-old Sprague-Dawley rats which were prepared by th...Aim The aim of this investigation was to evaluate gender differences in development of renovascular hy- pertension. Methods The experiments were performed with 10-week-old Sprague-Dawley rats which were prepared by the narrow side of the renal artery to development renovascular hypertension （2K1C）. After 32 weeks of regular feeding, rats were instrumented to determine blood pressure in anesthesia state. The blood was collecting for determi- nation of Anglland ET-1 by using ELISA kit. The thoracic aorta were isolated and snap frozen in liquid nitrogen. Ap- plications Western Blot method to detect tissue protein expression of angiotensin-converting enzyme （ACE1, ACE2）, and Angllreceptor（AT1 R, AT2 R）. Results SBP and DBP were higher in 2K1 C-females than in 2K1 C-males [ SBP： （ 172 ± 19. 2） vs （ 157 ± 16. 4） mmHg , P 〈0. 01. DBP： 138 ± 15.4 vs 128 ± 15.5, P 〈0. 05, n =20]. Plasma AngII were higher in 2K1C-males [ （ 11.2 ±2. 87） vs （9.60 ±3.51 ） ng · L^-1, P 〈0. 05] , and ET-1 were higher in 2K1C- females （38.77 ± 17.99 vs 33.76 ± 17.50 ng · L^-1, P 〈0. 05） . In thoracic aorta tissue, the protein levels of ACE1 and ACE2 were increased in 2K1C-females （ACE1 ： 1. 204 ＋0. 258 vsl. 182 ±0. 209, ACE2： 2. 431 ±0. 214 vs 1. 528±0.130, P 〈0.01）, AT1R were increased in 2K1C-males （0.955 ±0.201 vs 0.841 ±0.176, P 〈0.05）, ATzR were increased in 2K1C-females （2. 464±0. 142 vs 1. 431±0. 062, P 〈0. 01）. In kidney tissue, the protein levels of ACE1 were increased in 2K1C-females （2. 023 ＋0. 144 vs 1. 079±0. 103, P 〈0. 01）, ACE2 were increased in 2K1C- males （2. 496 ±0. 314 vs 1. 706±0. 214, P 〈0. 05）, AT2R and ATzR were increased in 2K1C-males （AT2R：0. 867 ±0. 092 vs O. 685±0. 176, P 〈0. 05, ATzR： 1. 413 ± 0. 233 vs 1. 146 ±0. 146, P 〈0. 05）. In heart tissue, the pro- tein levels of ACE1 were increased in 2K1 C-females （1. 122 ± 0. 177 vs O. 793± 0. 125, P 〈 0. 05） , ACE2 were no difference （0. 682 ±0. 108 vs O. 654 ±0. 103）, AT2R were increased in 2K1C-females （1. 478 ± 0. 235 vs 1. 223 ± 0. 193, P 〈0. 05）, ATzR were increased in 2K1C-males （ 1. 075 ± 0. 170 vs O. 924 ±0. 146, P 〈0. 05）. Conclu- sions The severity of hypertension was greater in 2K1 C-females than in 2K1 C-males. It is likely that the effect of fe- males on renovascular hypertension was depending on promoting the role of Ang II by increasing the release of ET-1,increasing the protein expression levels of ACE1 in thoracic aorta, kidney, and heart tissue, and increasing the pro- tein expression level of AT1 receptors in heart tissue.展开更多
Background The purpose of this study was to evaluate the effects of percutaneous transluminal renalr stenting (PTRS) on hypertension and renal function in patients with atherosclerotic renovascular disease.Methods A t...Background The purpose of this study was to evaluate the effects of percutaneous transluminal renalr stenting (PTRS) on hypertension and renal function in patients with atherosclerotic renovascular disease.Methods A total of 147 stents were deployed in 147 lesions of 135 consecutive patients for poorly controlled hypertension or preservation of renal function. Clinical follow-up of the effect of the procedure on renal function, blood pressure control, number of antihypertensive medications, and survival was performed in 128 (95%) patients after 22±14 months. Angiographic follow-up were performed in 70% of the patients at 7.24-5.6 months after PTRS. Results The immediate technical success was 100%. At 22±14 months, systolic and diastolic blood pressures significantly decreased (from 172±23 to 159±20 mm Hg and from 93±16 to 85±13 mm Hg, respectively; P<0.05). The number of antihypertensive medications was reduced on average by 0.74 (from 2.6±1.8 to 1.9±1.7, P<0.01). Among the 49 patients whose renal function was impaired initially (Serum creatinine concentration (SCC) >130 μmol/L), SCC was improved in 25%, became stabilized in 48% and continued to deteriorate in 27%. When SCC was <130 μtmol/L, 97% of the patients remained stabilized, while only 2 patients, SCC deteriorated by 22 months. The cumulative probability of survival was 96% (129/135) at 22 months, with 3 deaths related to end-stage renal disease. The in-stentrestenosis rate was 7.4% (7/95) at a mean follow up of 7.2±5.6 months.Conclusions In patients with atherosclerotic renal-artery stenosis, PTRS could beneficially affect blood pressure control and may improve or prevent further deterioration of renal function.展开更多
Objective To analyze the clinical features and effects of target therapy of post splenectomy pulmonary hypertension, and improve the diagnosis and treatment of the disease.Methods Clinical data of 18 patients with pos...Objective To analyze the clinical features and effects of target therapy of post splenectomy pulmonary hypertension, and improve the diagnosis and treatment of the disease.Methods Clinical data of 18 patients with post splenectomy pulmonary hypertension admitted to our hospital from October 2006 to March 2017 were systematically reviewed.展开更多
OBJECTIVE The current therapeutic approaches have a limited effect on the dysregulated pulmonary vascular remodeling,which is characteristic of pulmonary arterial hypertension(PAH).In this study we exam-ined whether s...OBJECTIVE The current therapeutic approaches have a limited effect on the dysregulated pulmonary vascular remodeling,which is characteristic of pulmonary arterial hypertension(PAH).In this study we exam-ined whether salvianolic acid A(SAA)extracted from the traditional Chinese medicine′Dan Shen′attenuated vascular remodeling in a PAH rat model,and elucidated the underlying mechanisms.METHODS PAH was induced in rats by injecting a single dose of monocrotaline(MCT 60 mg·kg-1,sc).The rats were orally treated with either SAA(0.3,1,3 mg·kg-1·d-1)or a positive control bosentan(30 mg·kg-1·d-1)for 4 weeks.Echocardiography and hemodynamic measurements were performed on d 28.Then the hearts and lungs were harvested,the organ indices and pulmonary artery wall thickness were calculated,and biochemical and histochemical analysis were conducted.The levels of apoptotic and signaling proteins in the lungs were measured using immunoblotting.RESULTS Treatment with SAA or bosentan effectively ameliorated MCTinduced pulmonary artery remodeling,pulmonary hemodynamic abnormalities and the subsequent increases of right ventricular systolic pressure(RVSP).Furthermore,the treatments significantly attenuated MCT-induced hypertrophic damage of myocardium,parenchymal injury and collagen deposition in the lungs.Moreover,the treatments attenuated MCT-induced apoptosis and fibrosis in the lungs.The treatments partially restored MCT-induced reductions of bone morphogenetic protein typeⅡreceptor(BMPRⅡ)and phosphorylated Smad1/5 in the lungs.CONCLUSION SAA ameliorates the pulmonary arterial remodeling in MCT-induced PAH rats most likely via activating the BMPRⅡ-Smad pathway and inhibiting apoptosis.Thus,SAA may have therapeutic potential for the patients at high risk of PAH.展开更多
Objective To investigate the risk factors associated with combined post-capillary and pre-capillary pulmonary hypertension(Cpc-PH)and the prognostic difference between isolated post-capillary pulmonary hypertension(Ip...Objective To investigate the risk factors associated with combined post-capillary and pre-capillary pulmonary hypertension(Cpc-PH)and the prognostic difference between isolated post-capillary pulmonary hypertension(Ipc-PH)and Cpc-PH in pulmonary hypertension due to left heart failure.Methods From October 2012 to November 2016,patients with pulmonary hypertension due to heart failure with preserved ejection fraction(PH-HFpEF)and pulmonary hypertension due to heart failure with reduced ejection fraction(PH-HFrEF)were prospectively enrolled from 11 centers all over the country.展开更多
Objective Hypoxemia is one of the main factors of pulmonary hypertension,and it can also be a complication of pulmonary hypertension,and it is a risk factor for many cardiovascular diseases,which increases the adverse...Objective Hypoxemia is one of the main factors of pulmonary hypertension,and it can also be a complication of pulmonary hypertension,and it is a risk factor for many cardiovascular diseases,which increases the adverse prognosis of patients.At present,there are some controversies about the diagnosis and treatment of sleep apnea disorder in patients with pulmonary hypertension.展开更多
Objective Hypertension is the most common chronic non-communicable disease responsible for the greatest disease burden in the world,as well as the most important public health issue in China.However no previous study ...Objective Hypertension is the most common chronic non-communicable disease responsible for the greatest disease burden in the world,as well as the most important public health issue in China.However no previous study on the awareness,treatment,and control of hypertension enrolled patients from every province in southwest China,only a few relatively small studies indicated the high prevalence and poor control of hypertension in this area.展开更多
Background and Purpose Recent studies found endothelialto-mesenchymal transition(EndoMT)played an important role in the pathogenesis of pulmonary arterial hypertension.Our pilot research demonstrated the existence of ...Background and Purpose Recent studies found endothelialto-mesenchymal transition(EndoMT)played an important role in the pathogenesis of pulmonary arterial hypertension.Our pilot research demonstrated the existence of Warburg effect in the lung tissue of idiopathic pulmonary arterial hypertension patients.However the relationships and the underlying mechanisms between EndoMT and Warburg effect have not been elucidated.Therefore,the purpose of this study is to determine whether metabolic reprogramming happens in EndoMT cells.We also want to investigate whether sodium dichloroacetate(DCA),a metabolic modulator,could prevent EndoMT by inhibiting Warburg effect.展开更多
Objective To summarize the clinical characteristics and the effect of pulmonary endarterectomy(PEA)in CTEPH patients with unilateral main pulmonary artery occlusion.Methods Of 160 CTEPH patients operated between Janua...Objective To summarize the clinical characteristics and the effect of pulmonary endarterectomy(PEA)in CTEPH patients with unilateral main pulmonary artery occlusion.Methods Of 160 CTEPH patients operated between January2004 and March 2018 at our center,13(8.1%)had complete main pulmonary artery occlusion.Patients were included if the ventilation/perfusion(V/Q)scan revealed nonperfusion of an entire lung and the pathological examination showed chronic thromboembolic.展开更多
Background The goal of this study was to explore the significance of left ventricular(LV)ejection/filling parameters in assessing severity and prognosis of pulmonary arterial hypertension(PAH).Methods Newly diagnosed ...Background The goal of this study was to explore the significance of left ventricular(LV)ejection/filling parameters in assessing severity and prognosis of pulmonary arterial hypertension(PAH).Methods Newly diagnosed PAH patients were recruited between July 2011 and December 2013 prospectively.Cardiopulmonary hemodynamics derived from right heart catheterization,and LV ejection/filling parameters derived from CMR were collected.Follow-up data were collected periodically using a specifically-designed network database until April 30,2016.展开更多
Objective To investigate the associations of handgrip strength with hypertension among Chinese Han population.Methods A total of 11, 151 subjects were recruited between October to November in 2015, using a multi-stage...Objective To investigate the associations of handgrip strength with hypertension among Chinese Han population.Methods A total of 11, 151 subjects were recruited between October to November in 2015, using a multi-stage sampling method in 13 townships of Jurong city, Jiangsu Province, south of China.展开更多
Objective Individuals with pulmonary hypertension(PH)have reduced exercise capacity and quality of life.Exercise-based rehabilitation may have beneficial effects to improve the exercise capacity and quality of life in...Objective Individuals with pulmonary hypertension(PH)have reduced exercise capacity and quality of life.Exercise-based rehabilitation may have beneficial effects to improve the exercise capacity and quality of life in patients with PH.We aim to assess the efficacy and safety of exercised-based rehabilitation for people eith PH by performing a randomized controlled trails(RCTs)meta-analysis of exercised-based rehabilitation for people with PH by performing a randomized controlled trails meta-analysis.展开更多
OBJECTIVE To explore the role of calpain in in pulmonary vascular remodeling in hypoxia induced pulmonary hypertension and the underlying mechanism.METHODS Sprague-Dawley rats were randomly divided into hypoxia group ...OBJECTIVE To explore the role of calpain in in pulmonary vascular remodeling in hypoxia induced pulmonary hypertension and the underlying mechanism.METHODS Sprague-Dawley rats were randomly divided into hypoxia group and normoxia control group.Right ventricular systolic pressure(RVSP)and mean pulmonary artery pressure(m PAP)were monitored by the method of right external jugular vein cannula.Right ventricular hypertrophy index was expressed as the ratio of right ventricular weight to left ventricular weight(left ventricle plus septum weight).Level of calpain-1,calpain-2and calpain-4 m RNA in pulmonary artery trunk were determined by real-time PCR.Expression of calpain-1,calpain-2 and calpain-4 protein was determined by Western Blot.Primary rat pulmonary arterial smooth muscle cells(PASMCs)were divided into 4 groups:normoxia control group,normoxia+MDL28170 group,hypoxia group and hypoxia+MDL28170 group.Cell proliferation was detected by MTS and flow cytometry.Level of Ki-67 and PCNA m RNA were determined by real-time PCR.RESULTS RVSP,m PAP and right ventricular remodeling index were significantly higher in the hypoxia group than those in the normoxia group.In the hypoxia group,pulmonary vascular remodeling occurred,and the expression of calpain-1,calpain-2 and calpain-4 m RNA and protein expression was increased in the pulmonary artery.MDL28170 significantly inhibited hypoxia-induced proliferation of PASMCs accompanied with decreased Ki-67and PCNA m RNA expression.CONCLUSION Calpain mediated vascular remodeling via promoting proliferation of PASMCs in hypoxia induced pulmonary hypertension.展开更多
Objective To investigate the prevalence of hypertension overweight/obesity and the combined effect on the incidence of cardiovascular disease(CVD).Methods The study population(aged from 35 to 64)were selected from 9 r...Objective To investigate the prevalence of hypertension overweight/obesity and the combined effect on the incidence of cardiovascular disease(CVD).Methods The study population(aged from 35 to 64)were selected from 9 regions of China by cluster sampling method.The baseline was conducted in 2010,and the follow-up survey was done in 2017.Participants with 24≤BMI28 kg/m^2 was defined as overweight,BMI≥28 kg/m^2 was defined as obesity.展开更多
Background and Objective Pulmonary arterial hypertension(PAH)is a pathophysiological disorder characterized by increased pulmonary arterial pressure and pulmonary vascular remodeling,its mechanism remains unclear.Tran...Background and Objective Pulmonary arterial hypertension(PAH)is a pathophysiological disorder characterized by increased pulmonary arterial pressure and pulmonary vascular remodeling,its mechanism remains unclear.Transforming growth factor-β(TGF-β)family plays a pivotal role in pulmonary arterial hypertension.SB525334 is a selective inhibitor of TGF-βreceptor 1,this study aimed to investigate the effects of SB525334 on the alleviation of monocrotalineinduced pulmonary hypertension.展开更多
文摘Objective Pulmonary arterial hypertension(PAH)is mainly characterized by pulmonary artery obstruction,which is diagnosed by a mean pulmonary artery pressure≥25 mm Hg at rest,and excluding other known causes of pulmonary hypertension.To identify genetic mutations and help make a precise diagnosis,we performed genetic testing in 191 probands with invasively confirmed PAH and tried to analyze the genotype-phenotype correlation.
文摘Background The reversibility of pulmonary arterial hypertension(PAH)in congenital heart disease(CHD)is of great importance for the operability of CHD.Proteomics analysis found that transgelin was significantly upregulated in the lung tissue of CHD-PAH patients,especially in the irreversible group.However,how exactly it participated in CHD-PAH development is unknown.
文摘Background and Objective Pulmonary hypertension (PH) is a fatal disease resulting from various causes. Studies from abroad have shown iron deficiency (ID) is closely associated with disease progression.This association is common in pulmonary arterial hypertension (PAH)that belongs to World Health Organization group 1 PH. However, ID prevalence in Chinese patients suffering from PH is unclear so far.
基金The project supported by National Natural Science Foundation of China(81274123,81374003)National Major Scientific and Technological Specialized Project for the Significant Formulation of New Drugs,China(2011ZX09101-002-07,2009ZX09502-016)
文摘OBJECTIVE Nowadays,with the living habits and diet structure changes,the morbidity and mortality of hypertension has been rising yearly,which induced by adverse lifestyles,excessive alcohol and poor diet.However,the animal models of hypertension resulted from adverse lifestyles have rarely been reported.It is essential to set up some more systematic models to study the characters of hypertension and evaluate the efficacy of traditional Chinese medicine(TCM).METHODS To build the rat model of human lifehabitual hypertension,SD rats had free access to alcohol with high-sucrose-fat diet(AHSFD),alcohol with high-fat diet(AHFD),high-sucrose-fat diet(HSFD),high-fat diet(HFD)and alcohol etc.Various of indicators were detected systematically to explore the features of hypertension models which included blood pressure(BP),liver and kidney functions,the lipid profiles and indexes quantifying TCM symptoms.Then we choose one of the stable and sustained animal models,studying the effects of RPA.E(which extracted from a TCM)on modulating the level of hypertension and the preliminary mechanism in this abstract.RESULTS ①The BP level,serum TC,TG,LDL-C of AHSFD-induced and AHFD-induced rats increased significantly,while the HDL-C reduced in the 4th week.② The BP level HSFD-induced rats increased significantly in the 6th week,serum TC,TG increased in 10 th week.③ The BP level of alcohol-induced rats increased significantly in the 9th week,serum TC,TG increased in 24 th week.④ The level of serum ALT,AST,UA and Cr of all model rats increased significantly after 12 weeks.Meanwhile the microcirculation increased significantly after8 weeks.⑤ After 4-week administration,RPA.E could significantly reduce BP of AHFD-induced hypertensive rats,and could reduce the serum TC,LDL-C,HDL-C,ALT,AST level after 8-week.CONCLUSION The rats induced by AHSFD,AHFD,HSFD and alcohol can raise BP obviously with underlying hepatic and kidney damage,the lipid profiles disorder and TCM symptoms change,while the AH(S)FD-induced hypertension models are earlier and more stable.RPA.E could mildly reduce BP level,and improve the lipid profiles disorders,hepatic damage,which reflects the characteristics of TCM on antihypertensive effects.The above models have their own characteristics,can be used to study the causes and pathogenesis of hypertension complicating metabolic disorder and the related treatment drug screening.
文摘Objective Studies of the association of hypertension with long-term exposure to particulate matter less than 2.5μm in diameter(PM2.5)have shown contradictory results and the magnitude of the association is still unclear.Hence,we conducted a meta-analysis of cohort studies to assess the effect of long-term exposure to PM2.5 on incident hypertension.
文摘Aim The aim of this investigation was to evaluate gender differences in development of renovascular hy- pertension. Methods The experiments were performed with 10-week-old Sprague-Dawley rats which were prepared by the narrow side of the renal artery to development renovascular hypertension （2K1C）. After 32 weeks of regular feeding, rats were instrumented to determine blood pressure in anesthesia state. The blood was collecting for determi- nation of Anglland ET-1 by using ELISA kit. The thoracic aorta were isolated and snap frozen in liquid nitrogen. Ap- plications Western Blot method to detect tissue protein expression of angiotensin-converting enzyme （ACE1, ACE2）, and Angllreceptor（AT1 R, AT2 R）. Results SBP and DBP were higher in 2K1 C-females than in 2K1 C-males [ SBP： （ 172 ± 19. 2） vs （ 157 ± 16. 4） mmHg , P 〈0. 01. DBP： 138 ± 15.4 vs 128 ± 15.5, P 〈0. 05, n =20]. Plasma AngII were higher in 2K1C-males [ （ 11.2 ±2. 87） vs （9.60 ±3.51 ） ng · L^-1, P 〈0. 05] , and ET-1 were higher in 2K1C- females （38.77 ± 17.99 vs 33.76 ± 17.50 ng · L^-1, P 〈0. 05） . In thoracic aorta tissue, the protein levels of ACE1 and ACE2 were increased in 2K1C-females （ACE1 ： 1. 204 ＋0. 258 vsl. 182 ±0. 209, ACE2： 2. 431 ±0. 214 vs 1. 528±0.130, P 〈0.01）, AT1R were increased in 2K1C-males （0.955 ±0.201 vs 0.841 ±0.176, P 〈0.05）, ATzR were increased in 2K1C-females （2. 464±0. 142 vs 1. 431±0. 062, P 〈0. 01）. In kidney tissue, the protein levels of ACE1 were increased in 2K1C-females （2. 023 ＋0. 144 vs 1. 079±0. 103, P 〈0. 01）, ACE2 were increased in 2K1C- males （2. 496 ±0. 314 vs 1. 706±0. 214, P 〈0. 05）, AT2R and ATzR were increased in 2K1C-males （AT2R：0. 867 ±0. 092 vs O. 685±0. 176, P 〈0. 05, ATzR： 1. 413 ± 0. 233 vs 1. 146 ±0. 146, P 〈0. 05）. In heart tissue, the pro- tein levels of ACE1 were increased in 2K1 C-females （1. 122 ± 0. 177 vs O. 793± 0. 125, P 〈 0. 05） , ACE2 were no difference （0. 682 ±0. 108 vs O. 654 ±0. 103）, AT2R were increased in 2K1C-females （1. 478 ± 0. 235 vs 1. 223 ± 0. 193, P 〈0. 05）, ATzR were increased in 2K1C-males （ 1. 075 ± 0. 170 vs O. 924 ±0. 146, P 〈0. 05）. Conclu- sions The severity of hypertension was greater in 2K1 C-females than in 2K1 C-males. It is likely that the effect of fe- males on renovascular hypertension was depending on promoting the role of Ang II by increasing the release of ET-1,increasing the protein expression levels of ACE1 in thoracic aorta, kidney, and heart tissue, and increasing the pro- tein expression level of AT1 receptors in heart tissue.
文摘Background The purpose of this study was to evaluate the effects of percutaneous transluminal renalr stenting (PTRS) on hypertension and renal function in patients with atherosclerotic renovascular disease.Methods A total of 147 stents were deployed in 147 lesions of 135 consecutive patients for poorly controlled hypertension or preservation of renal function. Clinical follow-up of the effect of the procedure on renal function, blood pressure control, number of antihypertensive medications, and survival was performed in 128 (95%) patients after 22±14 months. Angiographic follow-up were performed in 70% of the patients at 7.24-5.6 months after PTRS. Results The immediate technical success was 100%. At 22±14 months, systolic and diastolic blood pressures significantly decreased (from 172±23 to 159±20 mm Hg and from 93±16 to 85±13 mm Hg, respectively; P<0.05). The number of antihypertensive medications was reduced on average by 0.74 (from 2.6±1.8 to 1.9±1.7, P<0.01). Among the 49 patients whose renal function was impaired initially (Serum creatinine concentration (SCC) >130 μmol/L), SCC was improved in 25%, became stabilized in 48% and continued to deteriorate in 27%. When SCC was <130 μtmol/L, 97% of the patients remained stabilized, while only 2 patients, SCC deteriorated by 22 months. The cumulative probability of survival was 96% (129/135) at 22 months, with 3 deaths related to end-stage renal disease. The in-stentrestenosis rate was 7.4% (7/95) at a mean follow up of 7.2±5.6 months.Conclusions In patients with atherosclerotic renal-artery stenosis, PTRS could beneficially affect blood pressure control and may improve or prevent further deterioration of renal function.
文摘Objective To analyze the clinical features and effects of target therapy of post splenectomy pulmonary hypertension, and improve the diagnosis and treatment of the disease.Methods Clinical data of 18 patients with post splenectomy pulmonary hypertension admitted to our hospital from October 2006 to March 2017 were systematically reviewed.
基金Natural Science Foundation of China(81573645,81603101)the National Science and Technology Major Project(2013ZX09103001-008)
文摘OBJECTIVE The current therapeutic approaches have a limited effect on the dysregulated pulmonary vascular remodeling,which is characteristic of pulmonary arterial hypertension(PAH).In this study we exam-ined whether salvianolic acid A(SAA)extracted from the traditional Chinese medicine′Dan Shen′attenuated vascular remodeling in a PAH rat model,and elucidated the underlying mechanisms.METHODS PAH was induced in rats by injecting a single dose of monocrotaline(MCT 60 mg·kg-1,sc).The rats were orally treated with either SAA(0.3,1,3 mg·kg-1·d-1)or a positive control bosentan(30 mg·kg-1·d-1)for 4 weeks.Echocardiography and hemodynamic measurements were performed on d 28.Then the hearts and lungs were harvested,the organ indices and pulmonary artery wall thickness were calculated,and biochemical and histochemical analysis were conducted.The levels of apoptotic and signaling proteins in the lungs were measured using immunoblotting.RESULTS Treatment with SAA or bosentan effectively ameliorated MCTinduced pulmonary artery remodeling,pulmonary hemodynamic abnormalities and the subsequent increases of right ventricular systolic pressure(RVSP).Furthermore,the treatments significantly attenuated MCT-induced hypertrophic damage of myocardium,parenchymal injury and collagen deposition in the lungs.Moreover,the treatments attenuated MCT-induced apoptosis and fibrosis in the lungs.The treatments partially restored MCT-induced reductions of bone morphogenetic protein typeⅡreceptor(BMPRⅡ)and phosphorylated Smad1/5 in the lungs.CONCLUSION SAA ameliorates the pulmonary arterial remodeling in MCT-induced PAH rats most likely via activating the BMPRⅡ-Smad pathway and inhibiting apoptosis.Thus,SAA may have therapeutic potential for the patients at high risk of PAH.
文摘Objective To investigate the risk factors associated with combined post-capillary and pre-capillary pulmonary hypertension(Cpc-PH)and the prognostic difference between isolated post-capillary pulmonary hypertension(Ipc-PH)and Cpc-PH in pulmonary hypertension due to left heart failure.Methods From October 2012 to November 2016,patients with pulmonary hypertension due to heart failure with preserved ejection fraction(PH-HFpEF)and pulmonary hypertension due to heart failure with reduced ejection fraction(PH-HFrEF)were prospectively enrolled from 11 centers all over the country.
文摘Objective Hypoxemia is one of the main factors of pulmonary hypertension,and it can also be a complication of pulmonary hypertension,and it is a risk factor for many cardiovascular diseases,which increases the adverse prognosis of patients.At present,there are some controversies about the diagnosis and treatment of sleep apnea disorder in patients with pulmonary hypertension.
文摘Objective Hypertension is the most common chronic non-communicable disease responsible for the greatest disease burden in the world,as well as the most important public health issue in China.However no previous study on the awareness,treatment,and control of hypertension enrolled patients from every province in southwest China,only a few relatively small studies indicated the high prevalence and poor control of hypertension in this area.
文摘Background and Purpose Recent studies found endothelialto-mesenchymal transition(EndoMT)played an important role in the pathogenesis of pulmonary arterial hypertension.Our pilot research demonstrated the existence of Warburg effect in the lung tissue of idiopathic pulmonary arterial hypertension patients.However the relationships and the underlying mechanisms between EndoMT and Warburg effect have not been elucidated.Therefore,the purpose of this study is to determine whether metabolic reprogramming happens in EndoMT cells.We also want to investigate whether sodium dichloroacetate(DCA),a metabolic modulator,could prevent EndoMT by inhibiting Warburg effect.
文摘Objective To summarize the clinical characteristics and the effect of pulmonary endarterectomy(PEA)in CTEPH patients with unilateral main pulmonary artery occlusion.Methods Of 160 CTEPH patients operated between January2004 and March 2018 at our center,13(8.1%)had complete main pulmonary artery occlusion.Patients were included if the ventilation/perfusion(V/Q)scan revealed nonperfusion of an entire lung and the pathological examination showed chronic thromboembolic.
文摘Background The goal of this study was to explore the significance of left ventricular(LV)ejection/filling parameters in assessing severity and prognosis of pulmonary arterial hypertension(PAH).Methods Newly diagnosed PAH patients were recruited between July 2011 and December 2013 prospectively.Cardiopulmonary hemodynamics derived from right heart catheterization,and LV ejection/filling parameters derived from CMR were collected.Follow-up data were collected periodically using a specifically-designed network database until April 30,2016.
文摘Objective To investigate the associations of handgrip strength with hypertension among Chinese Han population.Methods A total of 11, 151 subjects were recruited between October to November in 2015, using a multi-stage sampling method in 13 townships of Jurong city, Jiangsu Province, south of China.
文摘Objective Individuals with pulmonary hypertension(PH)have reduced exercise capacity and quality of life.Exercise-based rehabilitation may have beneficial effects to improve the exercise capacity and quality of life in patients with PH.We aim to assess the efficacy and safety of exercised-based rehabilitation for people eith PH by performing a randomized controlled trails(RCTs)meta-analysis of exercised-based rehabilitation for people with PH by performing a randomized controlled trails meta-analysis.
基金The project supported by National Natural Science Foundation of China(81273512,81460010)by Natural Science Foundation of Jiangxi province(20142BAB215035)
文摘OBJECTIVE To explore the role of calpain in in pulmonary vascular remodeling in hypoxia induced pulmonary hypertension and the underlying mechanism.METHODS Sprague-Dawley rats were randomly divided into hypoxia group and normoxia control group.Right ventricular systolic pressure(RVSP)and mean pulmonary artery pressure(m PAP)were monitored by the method of right external jugular vein cannula.Right ventricular hypertrophy index was expressed as the ratio of right ventricular weight to left ventricular weight(left ventricle plus septum weight).Level of calpain-1,calpain-2and calpain-4 m RNA in pulmonary artery trunk were determined by real-time PCR.Expression of calpain-1,calpain-2 and calpain-4 protein was determined by Western Blot.Primary rat pulmonary arterial smooth muscle cells(PASMCs)were divided into 4 groups:normoxia control group,normoxia+MDL28170 group,hypoxia group and hypoxia+MDL28170 group.Cell proliferation was detected by MTS and flow cytometry.Level of Ki-67 and PCNA m RNA were determined by real-time PCR.RESULTS RVSP,m PAP and right ventricular remodeling index were significantly higher in the hypoxia group than those in the normoxia group.In the hypoxia group,pulmonary vascular remodeling occurred,and the expression of calpain-1,calpain-2 and calpain-4 m RNA and protein expression was increased in the pulmonary artery.MDL28170 significantly inhibited hypoxia-induced proliferation of PASMCs accompanied with decreased Ki-67and PCNA m RNA expression.CONCLUSION Calpain mediated vascular remodeling via promoting proliferation of PASMCs in hypoxia induced pulmonary hypertension.
文摘Objective To investigate the prevalence of hypertension overweight/obesity and the combined effect on the incidence of cardiovascular disease(CVD).Methods The study population(aged from 35 to 64)were selected from 9 regions of China by cluster sampling method.The baseline was conducted in 2010,and the follow-up survey was done in 2017.Participants with 24≤BMI28 kg/m^2 was defined as overweight,BMI≥28 kg/m^2 was defined as obesity.
文摘Background and Objective Pulmonary arterial hypertension(PAH)is a pathophysiological disorder characterized by increased pulmonary arterial pressure and pulmonary vascular remodeling,its mechanism remains unclear.Transforming growth factor-β(TGF-β)family plays a pivotal role in pulmonary arterial hypertension.SB525334 is a selective inhibitor of TGF-βreceptor 1,this study aimed to investigate the effects of SB525334 on the alleviation of monocrotalineinduced pulmonary hypertension.
